Electrochemiluminescence immunoassay for progesterone by using a heterologous system in plasma bovine

11. Electrochemiluminescence immunoassay for progesterone by using a heterologous system in plasma bovine - A. Ayad, M. Iguer Ouada and H. Benbarek
Veterinary World, 7(8): 610-613

   doi: 10.14202/vetworld.2014.610-613

A. Ayad: Department of Environment and Biological Sciences, Faculty of Life and Nature Sciences, University A. Mira, 06000, Bejaia, Algeria; Laboratory of Research on Local Animal Products, Veterinary Institute, Ibn Khaldoun University, 14000, Tiaret, Algeria; hanine06@gmail.com,abdelhanine.ayad@univ-bejaia.dz

M. Iguer Ouada: Department of Environment and Biological Sciences, Faculty of Life and Nature Sciences, University A. Mira, 06000,

Bejaia, Algeria; imokrane@gmail.com

H. Benbarek: Laboratory of Research on Local Animal Products, Veterinary Institute, Ibn Khaldoun University, 14000, Tiaret, Algeria; Department of Agricultural Sciences, Faculty of Life and Nature Sciences, University M. Istambouli, 29000, Mascara, Algeria; benbarekh@yahoo.com

Received: 12-05-2014, Revised: 27-07-2014, Accepted: 02-08-2014, Published online: 21-08-2014

Corresponding author: A. Ayad, email: hanine06@gmail.com, abdelhanine.ayad@univ-bejaia.dz

---

Abstract

---

Aim: The present study describes the use of electrochemiluminescence (ECL) immunoassay method with specific kit human progesterone for measuring plasma progesterone in cattle.

Materials and Methods: Nine Holstein-Friesian females were selected and artificially inseminated (AI). Blood samples were collected from the coccygeal vessels into tubes containing EDTA at day 0 (n = 5, the control group) and day 90 (n = 4, diagnosed pregnant by rectal palpation) after AI. The day of AI was considered as day 0 for the calculation of pregnancy day. The samples were immediately centrifuged (15 min at 1500 × g), and the plasma was stored at −20°C until assay. The assay of progesterone was carried out by a method of competition immunological type in heterogeneous phase. The antibodies of capture and revelation are monoclonal specific to progesterone of human origin.

Results: The progesterone (P4) concentration in the whole female was physiological. The results of inter- and intraassay coefficients of variation were 16.6% and 6.7%, respectively. The values of accuracy and parallelism obtained were satisfactory.

Conclusion: The preliminary results show clearly that human progesterone ECL kit can be used to measure P4 in plasma bovine.

Keywords: assay, cow, electochemiluminescence, progesterone.