3. Occurrence, species distribution and antimicrobial resistance of thermophilic Campylobacter isolates from farm and laboratory animals in Morogoro, Tanzania

3. Occurrence, species distribution and antimicrobial resistance of thermophilic Campylobacter isolates from farm and laboratory animals in Morogoro, Tanzania - Erick V. G. Komba, Robinson H. Mdegela, Peter L. M. Msoffe, Denis E. Matowo and Makori J. Maro

Veterinary World, 7(8): 559-565

   doi: 10.14202/vetworld.2014.559-565

Erick V. G. Komba: Department of Veterinary Medicine and Public Health, Faculty of Veterinary Medicine, Sokoine University
of Agriculture, P.O. Box 3021, Morogoro, Tanzania; babagrid@yahoo.com; ekomba@suanet.ac.tz

Robinson H. Mdegela: Department of Veterinary Medicine and Public Health, Faculty of Veterinary Medicine, Sokoine University
of Agriculture, P.O. Box 3021, Morogoro, Tanzania; rmdegela@yahoo.com

Peter L. M. Msoffe: Department of Veterinary Medicine and Public Health, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3021, Morogoro, Tanzania; Directorate of Undergraduate Studies, University of Dodoma, P. O. Box 259, Dodoma, Tanzania;makengamsoffe@yahoo.co.uk

Denis E. Matowo: Amana Regional referral Hospital, P.O. Box 25411, Dar es salaam, Tanzania; dmatowo@yahoo.com

Makori J. Maro: Ngudu District Hospital, P.O. Box 64, Ngudu/Kwimba, Mwanza, Tanzania; marojosephat@yahoo. com

Received: 09-04-2014, Revised: 17-06-2014, Accepted: 24-06-2014, Published online: 03-08-2014

Corresponding author: Erick V. G. Komba, email: babagrid@yahoo.com; ekomba@suanet.ac.tz

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Abstract

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Aim: To determine the carriage and antimicrobial resistance of Thermophilic Campylobacter species in the gastrointestinal tracts of farm and laboratory animals in Morogoro, Tanzania
Materials and Methods: Faecal samples were collected from farm (n=244) and laboratory (n=466) animals and were subjected to the Cape Town protocol for isolation of Campylobacter. Isolates were preliminarily identified based on potassium hydroxide string and hippurate hydrolysis tests. Polymerase chain reaction (PCR) was employed for confirmation of isolates. Antimicrobial resistance testing was done using disc diffusion method.
Results: Of the laboratory animals 26.7% of guinea pigs (n=30) and 1.2% of rats (n=242) were colonized with Campylobacter. Four isolates from guinea pigs were Campylobacter jejuni and the other four were C. coli. From rats, two isolates were C. jejuni and one was C. coli. In farm animals thermophilic Campylobacter were detected from 31.6% of sheep (n=57) and 60% horses (n=5). Of the isolates 12 (57%) were C. jejuni (10 from sheep and 2 from horses) and the remaining were C. coli (8 from sheep and 1 from a horse). The isolates were frequently resistant to erythromycin, norfloxacin, colistin sulphate and nalidixic acid; whereas low levels of resistance were observed for ciprofloxacin and gentamicin.
Conclusion: Our study reveals carriage of antimicrobial resistant thermophilic Campylobacter in the intestines of the study animals. This highlights possibilities in involvement of these animals in the epidemiology of Campylobacter infections. Thus, there is a need to consider these animal species when planning control measures for this zoonotic bacterium.
Keywords: campylobacteriosis, cape town protocol, farm animals, laboratory animals.

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