Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture

Research (Published online: 18-04-2018)

15. Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture

Chiquita Prahasanti, Denny Tri Wulandari and Noer Ulfa

Veterinary World, 11(4): 506-510

Aim: This study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21.

Materials and Methods: Collagen was extracted from gouramy fish scales (O. gouramy) with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. Its morphology then was analyzed with scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay was conducted to compare cells with and without fish scale collagen treatment.

Results: Collagen extracted from gouramy fish scales had no influence statistically on cultured fibroblast cells with a statistical significance (2-tailed) value of 0.754 (p>00025).

Conclusion: Collagen extracted from gouramy fish scales has high viability against BHK21 fibroblast cells.

Keywords: bone graft, collagen, gouramy fish scale, viability, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide.