Evaluation of antimycobacterial activity of Curcuma xanthorrhiza ethanolic extract against Mycobacterium tuberculosis H37Rv in vitro

Research (Published online: 27-03-2018)

18. Evaluation of antimycobacterial activity of Curcuma xanthorrhiza ethanolic extract against Mycobacterium tuberculosis H37Rv in vitro

Ngadino, Setiawan, Koerniasari, Ernawati and S. A. Sudjarwo

Veterinary World, 11(3): 368-372

ABSTRACT

Aim: The aim of this study was to evaluate the antimycobacterial activity of the Curcuma xanthorrhiza ethanolic extract in vitro.

Materials and Methods: Ethanolic extract of C. xanthorrhiza was set by maceration method. The broth microdilution and disc diffusion method were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), respectively, of C. xanthorrhiza ethanol extract on strain Mycobacterium tuberculosis H37Rv.

Results: C. xanthorrhiza ethanol extract was found to have the antimycobacterial effects with a MIC value of 1600 μg/ml while MBC value of 3200 μg/ml for M. tuberculosis H37Rv.

Conclusion: From these findings , it can be concluded that C. xanthorrhiza ethanol extract have an antibacterial activity against Mycobacterium tuberculosis H37Rv in vitro and its potency elevated by increasing the C. xanthorrhiza ethanol extract concentration.

Keywords: antimycobacterial, Curcuma xanthorrhiza, minimal bactericidal concentration, minimal inhibitory concentration.