Research (Published online: 04-06-2017)
4. Prevalence of methicillin-resistant Staphylococcus aureus skin and nasal carriage
isolates from bovines and its antibiogram
isolates from bovines and its antibiogram
Alok Kumar, Purushottam Kaushik, Anjay, Pankaj Kumar and Manoj Kumar
Veterinary World, 10(6): 593-597
Aim: This study was conducted to determine the
prevalence of methicillin-resistant
Staphylococcus aureus (MRSA) in cattle and buffalo and to study their antibiotic
resistance pattern.
Staphylococcus aureus (MRSA) in cattle and buffalo and to study their antibiotic
resistance pattern.
Materials
and Methods: A total
of 136 samples (skin and nasal swab) from cattle and buffalo
were collected. MRSA was identified by conventional bacterial culture techniques which were
further confirmed by amplification of S. aureus-specific 16S rRNA by polymerase chain reaction
(PCR). The isolates were further analyzed for the presence of mecA gene by PCR. The
antimicrobial susceptibility profiling was performed by disc diffusion method.
were collected. MRSA was identified by conventional bacterial culture techniques which were
further confirmed by amplification of S. aureus-specific 16S rRNA by polymerase chain reaction
(PCR). The isolates were further analyzed for the presence of mecA gene by PCR. The
antimicrobial susceptibility profiling was performed by disc diffusion method.
Results: The prevalence of MRSA in the current study
was 28.57% and 34.28% in cattle nasal
and skin swab, respectively, with an overall prevalence of 31.43% MRSA among cattle. Buffalo
nasal and skin sample showed MRSA prevalence of 54.55% and 39.4%, respectively, with 46.9%
overall prevalence. PCR could detect mecA gene in 36.4% and 58% MRSA isolates from cattle
and buffalo, respectively. Antimicrobial susceptibility test found MRSA resistant to penicillin and
oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole (62%), and amoxyclav (50%).
100% sensitivity was observed against ciprofloxacin, amikacin, chloramphenicol, and gentamicin.
Three (16.7%) MRSA isolates from buffalo were found resistant to vancomycin.
and skin swab, respectively, with an overall prevalence of 31.43% MRSA among cattle. Buffalo
nasal and skin sample showed MRSA prevalence of 54.55% and 39.4%, respectively, with 46.9%
overall prevalence. PCR could detect mecA gene in 36.4% and 58% MRSA isolates from cattle
and buffalo, respectively. Antimicrobial susceptibility test found MRSA resistant to penicillin and
oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole (62%), and amoxyclav (50%).
100% sensitivity was observed against ciprofloxacin, amikacin, chloramphenicol, and gentamicin.
Three (16.7%) MRSA isolates from buffalo were found resistant to vancomycin.
Conclusion: Cattle and buffalo were identified as a
potential carrier of MRSA in Bihar (India).
The isolation of
vancomycinresistant S. aureus (VRSA) in the
current study indicates the
emergence of VRSA in animal population which may be transmitted to the human beings
working in close contact to the animals.
emergence of VRSA in animal population which may be transmitted to the human beings
working in close contact to the animals.
Keywords: antibiogram, bovine, mecA gene, methicillin
resistant Staphylococcus
aureus.
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