Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle

Research (Published online: 03-02-2016)

1. Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle - Sandip Bhat, Pushpendra Kumar, Neeraj Kashyap, Bharti Deshmukh, Mahesh Shivanand Dige, Bharat Bhushan, Anuj Chauhan, Amit Kumar and Gyanendra Singh

Veterinary World, 9(2): 113-117

   doi: 10.14202/vetworld.2016.113-117

Abstract

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Aim: Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle.

Materials and Methods: A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-singlestrand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons.

Results: Three SSCP patterns and consequently two alleles namely A and B were documented in one fragment of HSP70 gene. On sequencing, one single-nucleotide polymorphism with G > T substitution was found at a position that led to a change of amino acid aspartate to tyrosine in allele A. It was found that in maintaining near normal average rectal temperature, genotype AA was superior (p≤0.01). Genotype AA, thus, was found to be most thermotolerant genotype with the highest HTC (p≤0.01).

Conclusion: The polymorphism at HSP70 is expected to be a potent determinant for heat tolerance in cattle, which may aid in selection for thermotolerance in cattle.

Keywords: cattle, heat tolerance, heat shock protein 70, polymorphism, Tharparkar.