Research (Published online: 15-12-2014)
10. Cloning and sequence analysis of hsf, an outer membrane protein gene of Pasteurella multocida serotype B:2 - A. Priyadarshini, S. Kumar, S. K. Gupta, K. N. Viswas, R. K. Agarwal and V. P. Singh
Veterinary World, 7(12): 1090-1093
doi: 10.14202/vetworld.2014.1090-1093
Aim: The present study was undertaken to clone, sequence and analyze the hsf, an outer membrane protein gene of Pasteurella multocida serotype B:2
Materials and Methods: hsf gene was amplified from genomic DNA of P. multocida. Polymerase chain reaction (PCR) product was cloned in pET-32a vector and was characterized. hsf gene was sequenced, analyzed and phylogenetic tree was constructed taking sequences of other strains.
Results: Amplicon size was found to be 785 bp. Recombinant got characterized through colony PCR and restriction enzyme analysis.
Conclusion: hsf gene of P. multocida serotype B is similar to serotype A, but different from serotype D. Further work is needed to evaluate role of Hsf protein in protection studies and to study the antigenic properties of this recombinant protein as a candidate for vaccine.
Keywords: cloning, hsf gene, outer membrane protein, Pasteurella multocida, phylogenetic tree, sequencing
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