16. Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen - S. S. Ramteke, A. M. Shende,
J. S. Rajoriya, N. C. Barik, Megha Pande, S. K. Bhure, G. K. Das, S. K. Ghosh and J. K. Prasad
Veterinary World, 7(9): 707-711
doi: 10.14202/vetworld.2014.707-711
S. S. Ramteke: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;drsnehalramteke@rediffmail.com
A. M. Shende: Protein Research Laboratory, Animal Biochemistry Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; asashishivri216@gmail.com
J. S. Rajoriya: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;jituivri@gmail.com
N. C. Barik: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;iamdrncbarik@gmail.com
Megha Pande: Animal Physiology Lab, Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India; megha985@gmail.com
S. K. Bhure: Protein Research Laboratory, Animal Biochemistry Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;sdbhure@rediffmail.com
G. K. Das: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;gkdasivri@gmail.com
S. K. Ghosh: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;subrata_kghosh@yahoo.co.in
J. K. Prasad: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;jkprasad2001@yahoo.co.in
Received: 12-06-2014, Revised: 31-07-2014, Accepted: 06-08-2014, Published online: 20-09-2014
Corresponding author: S. S. Ramteke, e-mail: drsnehalramteke@rediffmail.com
Aim: The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull.
Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of ≥3+ were used for the study and categorized into three groups (Group I- Mass motility 3+, Group II- Mass motility 4+ and Group III- Mass motility 5+). Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry’s method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls.
Results: The mean values of TSPP concentrations in bull no. 605 and 790 in Group I, II and III were 30.64±0.12, 31.66±0.09, 32.53±0.19 and 28.51±0.09, 29.49±0.15, 30.45±0.17 mg/mL, respectively. The mean values of HBPs concentrations in bull no. 605 and 790 in Group I, II and III were 3.11±0.07, 3.32±0.06, 3.46±0.08 and 2.51±0.08, 2.91±0.05, 3.10±0.03 mg/mL, respectively. Both the values of TSPP and HBPs were significantly higher (p<0.01) in bull no. 605 when compared to 790 in all the three groups. 31 kDa HBP was more intensely present in bull no. 605, thus may indicate its superiority over bull no. 790 in relation to fertility potential.
Conclusion: TSPP and HBPs shows variation in concentration with respect to initial semen quality. Furthermore, presence of fertility related 31 kDa HBPs in one of the bull may be an indication of high fertility of a bull. In future, in-vivo and in-vitro correlative study on larger basis is needed for the establishment of fertility-related HBPs in semen which might establish criteria for selection of buffalo bull with high fertility potential.
Keywords: buffalo bull, heparin-binding proteins, semen, seminal plasma.
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