Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio

15. Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio - J. S. Rajoriya, J. K. Prasad, S. K. Ghosh, Snehal S. Ramteke, N. C. Barik, G. K. Das and Megha Pande

Veterinary World, 7(9): 702-706

   doi: 10.14202/vetworld.2014.702-706

J. S. Rajoriya: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India; jituivri@gmail.com

J. K. Prasad: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India; jkprasad2001@yahoo.com

S. K. Ghosh: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India; subrata_kghosh@yahoo.co.in

Snehal S. Ramteke: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India; drsnehalramteke@rediffmail.com

N. C. Barik: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India; iamdrncbarik@gmail.com

G. K. Das: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India; gkdasivri@gmail.com

Megha Pande: Animal Physiology Lab, Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India; megha985@gmail.com

Received: 24-05-2014, Revised: 02-08-2014, Accepted: 06-08-2014, Published online: 20-09-2014

Corresponding author: J. S. Rajoriya, e-mail: jituivri@gmail.com

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Abstract

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Aim: The study was conducted to investigate the effect of cholesterol loaded cyclodextrin (CLC) on freezability of buffalo spermatozoa.

Materials and Methods: Murrah buffalo bull semen samples with progressive motility of 70% and greater were used. After the evaluation of motility and livability, four equal fractions of semen samples were made. Group I was kept as control and diluted with Tris, whereas Group II, III and IV were treated with CLC solution at the rate of 2.0, 3.0 and 4.0 mg/ml respectively to obtain 120 × 106 sperm/ml as final spermatozoa concentration. The aliquots of all the groups were incubated for action of CLC, followed by dilution and freezing. Evaluation at pre-freeze and post-thaw stage of progressive motility, viability and level of cholesterol and phospholipid was done.

Results: The mean cholesterol content (μg/100 × 106 spermatozoa) of Group I, II, III and IV at pre-freeze stage was 21.55±0.63, 49.56±1.38, 55.67±0.45 and 47.79±1.01 and at post-thaw stage were 13.18±0.45, 34.27±0.71, 36.21±0.48 and 33.68±0.56, respectively. At pre-freeze stage, cholesterol content was significantly (p<0.01) higher in Group III in comparison to other groups. The mean cholesterol and phospholipids content of fresh sperm was 24.14±0.58 and 51.13±0.66 μg/100 × 106 sperm cells, respectively, and C/P ratio of spermatozoa at fresh stage was 0.47±0.067.

Conclusion: CLC treatment maintains the C/P ratio and plays an important role in maintaining membrane architecture of spermatozoa. Hence, addition of CLC may be helpful in increasing freezability of buffalo spermatozoa by increasing the C/P ratio of spermatozoa.

Keywords: buffalo, cholesterol loaded cyclodextrin, cholesterol:phospholipid (C/P) ratio.