Sensitivity of RNA viral nucleic acid-based detection of avian influenza virus, Newcastle disease virus, and African horse sickness virus on flinders technology associates card using conventional reverse-transcription polymerase chain reaction

Research (Published online: 30-11-2022)

28. Sensitivity of RNA viral nucleic acid-based detection of avian influenza virus, Newcastle disease virus, and African horse sickness virus on flinders technology associates card using conventional reverse-transcription polymerase chain reaction

Khate Rattanamas, Machimaporn Taesuji, Usakorn Kulthonggate, Tippawan Jantafong, Thanongsak Mamom, and Sakchai Ruenphet

Veterinary World, 15(11): 2754-2759

ABSTRACT

Background and Aim: The flinders technology associates (FTA) card is a cotton-based cellulose membrane impregnated with a chaotropic agent that inactivates infectious microorganisms, lyses cellular material, and fixes DNA and/or RNA within the fiber matrix. However, little is known about the effectiveness of these cards for detecting RNA viruses in animals. This study aimed to evaluate the sensitivity of RNA virus detection using conventional reverse-transcription polymerase chain reaction (RT-PCR) on FTA cards.

Materials and Methods: A highly virulent Newcastle disease virus (NDV) and an avian influenza virus (AIV) with low pathogenicity were propagated using chicken embryonic eggs. Three days after inoculation, the allantoic fluid was harvested, stored at –80°C, and the stock virus was tested for virus titration. African horse sickness virus (AHSV) was obtained from a live attenuated vaccine that was dissolved and stored at –80°C. For sample preparation, each stock virus was 10-fold serially diluted and each dilution was inoculated onto an FTA card, followed by drying in a Class II safety cabinet. Both the stock virus and infected FTA card were genomically isolated using an extraction kit, FTA purification kit, and extraction kit with Tris-EDTA (TE) buffer. The target genome was then detected by one-step RT-PCR for NDV and AIV, and two-step RT-PCR for African horse sickness, including gel electrophoresis for the detection of specific nucleic acids.

Results: The detection limit of stock AIV was compared on FTA cards, using the FTA purification kit, and with TE buffer with an extraction kit. The corresponding results were 1.47, 1.17, and 2.18 log10 EID50, respectively, while for NDV the results were 4.13, 4.83, and 4.84 log10 ELD50. Finally, detection limit of stock AHSV and AHSV on the FTA card extracted using TE buffer with an extraction kit were 4.30 and 4.01 log10 plaque-forming units, respectively.

Conclusion: This study demonstrated that the detection limit or sensitivity of all tested RNA viruses on FTA cards did not differ when compared with those of the stock virus and in both methods for RNA isolation on FTA cards. These cards are suitable for collecting and transporting samples infected with RNA viruses, particularly AIV, NDV, and AHSV. Flinders technology associates cards also provide hazard-free samples, a reliable source of RNA for molecular characterization, and sufficient quantity for diagnostic applications based on nucleic acid-based detection.

Keywords: African horse sickness virus, avian influenza virus, flinders technology associates card, Newcastle disease virus, sensitivity.

Zoonotic diseases risk perception and infection prevention and control practices among poultry farmers in the Buea Health District, Cameroon: A one health perspective

Research (Published online: 30-11-2022)

27. Zoonotic diseases risk perception and infection prevention and control practices among poultry farmers in the Buea Health District, Cameroon: A one health perspective

Marie Ebob Agbortabot Bissong, Johnny Castro Nganjo Lyombe, Emmanuel Asongalem, Robert Bongji Ngamsha, and Nicholas Tendongfor

Veterinary World, 15(11): 2744-2753

ABSTRACT

Background and Aim: Livestock are associated with pathogenic microbes and farm workers play a significant role in the transmission of zoonotic diseases (ZDs). Lack of awareness of exposure risk among farmers may influence their farm practices thereby enhancing the spread of diseases on farms and to the community. This study was aimed at evaluating the knowledge, risk perception, and prevention and control practices of ZDs among poultry farmers to provide baseline data for establishing a "One Health" practical approach to reducing ZD transmission in poultry farms.

Materials and Methods: Using the exponential discriminative snowball technique, a community-based cross-sectional study involving poultry farmers was carried out in the Buea Health District from April to July 2021. Six feed-producing mills were used as focal points to identify and recruit farmers who also referred to other farmers. Questionnaires were used to collect data related to participants' knowledge, risk perception, and prevention and control practices of ZDs. Descriptive analyses were performed for all variables while the chi-square test and logistic regression analysis were used to determine associations at 95% confidence level.

Results: In all, 183 poultry farms and 207 workers were enrolled in the study. Despite being aware that animal diseases can be transmitted to humans, most participants showed poor knowledge (54.6%), low-risk perception (51.7%), and poor prevention/control practices (54.1%) on ZDs. The majority did not consider coming in contact with birds' body fluid (blood) or apparently healthy birds to be a risk of infection. More participants with small farms (<500 birds) had low-risk perception of ZDs than those with larger farms (>1000 birds) (p = 0.03). Furthermore, most participants reported practicing hand washing but they neither used protective devices such as gloves and face masks, and >50% would not invite veterinary professionals to their farms. There was a significant association between risk perception and knowledge (p = 0.007; CI = 1.257–4.200) as well as between risk perception and prevention/control practice (p = 0.002; CI = 1.451–4.867).

Conclusion: Poultry farm workers in Buea had poor knowledge and perception of ZD risk and this might have contributed to their poor prevention/control practices on the farms. Enhanced informal education of poultry farmers through training workshops and seminars will improve their knowledge and skills on ZD transmission risk and prevention.

Keywords: Cameroon, control practices, knowledge, poultry farmers, risk perception, Zoonotic diseases.

Association of natural resistance-associated macrophage protein 1 polymorphisms with Salmonella fecal shedding and hematological traits in pigs

Research (Published online: 29-11-2022)

26. Association of natural resistance-associated macrophage protein 1 polymorphisms with Salmonella fecal shedding and hematological traits in pigs

Nattariga Suwannawong, Uschara Thumarat, and Pitchayanipa Phongphanich

Veterinary World, 15(11): 2738-2743

ABSTRACT

Background and Aim: Natural resistance-associated macrophage protein 1 encoding gene (Nramp1) plays a role in immune response and disease resistance. This study aimed to investigate the polymorphisms of Nramp1 intron 6 concerning Salmonella shedding and hematological traits in pigs.

Materials and Methods: A total of 40 commercial pigs (three-way Large White x Landrace x Duroc cross) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and analyze the relationship between the polymorphisms of the Nramp1 gene and Salmonella fecal shedding and hematological parameters.

Results: Nramp1 was shown to be polymorphic in these pigs. The Nramp1 gene has two alleles (A and B) and two genotypes (AB and BB). The BB genotype had a higher frequency than the AB genotype. A significant relationship between the BB genotype and the number of Salmonella in feces compared to the AB genotype (p < 0.05) on 7 days post-inoculation (DPI) was revealed in the association analysis. The single-nucleotide polymorphism at intron 6 in the Nramp1 gene was linked to white and red blood cells 2 and 7 DPI (p < 0.05).

Conclusion: The Nramp1 gene was suggested by these findings to be potentially used as a molecular marker for the genetic selection of disease susceptibility in pig breeding.

Keywords: hematological trait, Nramp1, Salmonella shedding pig.

In vitro phytochemical analysis and antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi

Research (Published online: 29-11-2022)

25. In vitro phytochemical analysis and antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi

Maged A. Al-Garadi, Mohammed M. Qaid, Abdulmohsen H. Alqhtani, Anthony Pokoo-Aikins, and Saud I. Al-Mufarrej

Veterinary World, 15(11): 2725-2737

ABSTRACT

Background and Aim: Scientists are interested in identifying natural antibiotic substitutes that are effective against drug-resistant pathogenic microbes and spoilage fungi to counter pathogens and reduce the major public health problem of antibiotic residues in animal products. This study aimed to evaluate the antimicrobial activity of Rumex nervosus leaves (RNL) as a medicinal herb against four bacterial and two fungal strains using absolute ethanol, 50% ethanol, and aqueous extracts.

Materials and Methods: The antimicrobial activities of various RNL extracts against selected microbes were evaluated using the disk diffusion antibiotic susceptibility test, minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum fungicidal concentrations, and the poisoned food technique.

Results: The absolute ethanol RNL extract showed the best bacteriostatic/bactericidal activity against Salmonella Typhimurium, Escherichia coli, and Staphylococcus aureus (MIC/MBC: 0.20/0.40, 0.20/0.40, and 0.32/0.65 mg/mL, respectively). The diameter of the zone of inhibition was larger (p < 0.05) for the 100% ethanol RNL extract (8.17 mm) against Salmonella Typhimurium, the 50% ethanol-RNL extract (11.5 mm) against E. coli, and the aqueous RNL extract (14.0 mm) against S. aureus than for any other bacterial isolate. The aqueous RNL extract strongly (p < 0.0001) inhibited the mycelial growth of Aspergillus fumigatus (100%) and Aspergillus niger (81.4%) compared with the control.

Conclusion: The results of this study suggest that RNL is a promising new natural antimicrobial agent for food preservation. To date, most research on the antimicrobial properties of natural herbs has been conducted in vitro, with few exceptions in vivo and intervention-based research.

Keywords: antimicrobial activity, Aspergillus spp., Gram-negative bacteria, Gram-positive bacteria, phytochemicals, Rumex nervosus leaves.

The effects of mulberry (Morus alba Linn.) leaf supplementation on growth performance, blood parameter, and antioxidant status of broiler chickens under high stocking density

Research (Published online: 28-11-2022)

24. The effects of mulberry (Morus alba Linn.) leaf supplementation on growth performance, blood parameter, and antioxidant status of broiler chickens under high stocking density

Charinya So-In and Nuchsupha Sunthamala

Veterinary World, 15(11): 2715-2724

ABSTRACT

Background and Aim: A stocking density system in boilers is well known for increasing productivity. However, this system increases stress and affects the growth performance of broilers. Mulberry is a valuable plant with therapeutic applications in traditional medicine; moreover, it reduces free radicals and improves growth performance in broilers. This study was conducted to investigate the effects of mulberry on the blood biochemistry parameters and the antioxidant status of broilers exposed to various raising systems.

Materials and Methods: Two hundred and seventy-six 3-week-old male broilers were randomly assigned to nine categories composed of three growing systems: Semi-intensive, low stocking density, and high stocking density. Each group was fed with a control diet mixed with and without 10% mulberry leaf extract; the positive control group was provided with vitamin C. During the study, phytochemical screening of mulberry leaf extract, growth performances, hematological parameters, and antioxidant profiles were measured over the 4 weeks of the treatment.

Results: In the high stocking density group, lipid peroxidation gradually increased while antioxidant activities decreased; however, the level of lipid peroxidation was reduced, whereas catalase and superoxide dismutase activities were significantly increased. The growth performance and blood biochemistry were improved after being fed with 10% mulberry leaf extract.

Conclusion: This finding indicates that mulberry leaf extract reduced oxidative stress, activated antioxidant enzyme activities, and enhanced broilers' growth performance when raised under stress conditions.

Keywords: antioxidant, broiler chickens, growth performance, high stocking density, Morus alba, mulberry leaf.

Meta-analysis of the potential of Kazakhstani pork in the global market of meat products: Problems and prospects

Research (Published online: 28-11-2022)

23. Meta-analysis of the potential of Kazakhstani pork in the global market of meat products: Problems and prospects

Gulmira Karimzhanovna Dambaulova, Svetlana Ivanovna Lilimberg, Vladimir Anatoliyevich Madin, and Gainesh Turemuratovna Abdrakhmanova

Veterinary World, 15(11): 2705-2714

ABSTRACT

Background and Aim: Pig farming is integral in developing Kazakhstan's animal husbandry as it has shown rapid growth in profit and a high turnover. Industrial pig products have significant global demand, particularly in China and Russia. However, as pig farming is a small-scale industry with insufficient mechanization and automation in Kazakhstan, the costs have increased while the quality of Kazakhstani pig products has decreased due to the simultaneous influx of cheap imported pork into the domestic market. This study aimed to analyze the export potential of the swine industry in the Republic of Kazakhstan and assess its impact on the global pork market.

Materials and Methods: A meta-analysis of open sources was conducted for the period 1990–2020, while forecasting was extended to 2025. Statistical methods for the analysis (construction of time series with equal intervals, calculation, interpretation of average values, and growth rates) were used. Pearson correlation analysis was performed to study the dependence of the productivity of pigs on the category of the pig farming enterprise to establish the relationship between the average live weight of one head of pigs and the share of pigs in agricultural enterprises in the total number of pigs. The polynomial smoothing method was used, and a trend line was built, forecasting the number of pigs in Kazakhstan, the production and sale of pig products, along with their cost and profitability.

Results: This study briefly characterizes the developing situation of the global pork market. We identified and evaluated the development trends in Kazakh pig farming in terms of their impact on the industry's export potential. Specifically, we determined the following trends: An increase in the actual and projected number of pigs; a decrease in the natural loss of pigs during 2014–2020; the growth of existing and forecast indicators of pork sales in the domestic market; a steady decrease in the share of pigs in small households and an increase in the share of pigs in peasant farms and agricultural enterprises; an increase in the dynamics of the productivity of the pig population; and the growth of actual and predicted profitability of production and sale of pig products. We discussed the main problems that hinder the entry of Kazakhstani pork into the global market: Small-scale farming, insufficient mechanization and automation, and inadequate financial support from the state and banking structures. First, small-scale farming (deduced from the number of pigs by the farm categories) has hampered the small farms' independent manufacture and use of feed grain (i.e., barley, which is abundant in Kazakhstan), which might reduce costs. Moreover, small-scale farms struggle to comply with the waste disposal rules for pig production and environmental safety norms, which limit the export potential of Kazakhstani pork. Second, insufficient mechanization and automation in feeding and breeding (i.e., use of resource-saving technologies) result in inadequate productivity, decreased competitiveness, and failure to meet global standards. Third, insufficient financial support in the form of grants allocated to purposes other than selection and breeding limits the overall development of Kazakhstani pig farming.

Conclusion: The positive dynamics of the main development indicators of Kazakhstani pig farming efficiently assess the industry's export potential, according to the country's leading academic specialists and practitioners in the agricultural sector, especially in light of the difficult epizootic situation. This provides abundant opportunities for export supplies in the global meat market. These indicators include the number of pigs, its natural decline, and structure by farm categories. They also include the production, sale, and profitability of production of pig products and the productivity of pigs. As China and Russia currently have a massive shortage of this product in the domestic markets, they are potential importers of Kazakh pork.

Keywords: livestock size, pig farming, pig productivity, pig products, pork export, the profitability of pig farming.

Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant Staphylococcus aureus isolated from healthy rabbits

Research (Published online: 27-11-2022)

22. Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant Staphylococcus aureus isolated from healthy rabbits

Arunee Jangsangthong, Nawarat Suriyakhun, Witawat Tunyong, Thida Kong-Ngoen, Sirijan Santajit, Nitaya Indrawattana, and Shutipen Buranasinsup

Veterinary World, 15(11): 2699-2704

ABSTRACT

Background and Aim: Methicillin-resistant globally, Staphylococcus aureus (MRSA) is a major cause of disease in both humans and animals. Several studies have documented the presence of MRSA in healthy and infected animals. However, there is less information on MRSA occurrence in exotic pets, especially healthy rabbits. This study aimed to look into the antimicrobial resistance profile, hidden antimicrobial-resistant genes in isolated bacteria, and to estimate prevalence of MRSA in healthy rabbits.

Materials and Methods: Two-hundreds and eighteen samples, including 42 eyes, 44 ears, 44 oral, 44 ventral thoracic, and 44 perineal swabs, were taken from 44 healthy rabbits that visited the Prasu-Arthorn Animal Hospital, in Nakornpathom, Thailand, from January 2015 to March 2016. The traditional methods of Gram stain, mannitol fermentation, hemolysis on blood agar, catalase test, and coagulase production were used to confirm the presence of Staphylococcus aureus in all specimens. All bacterial isolates were determined by antimicrobial susceptibility test by the disk diffusion method. The polymerase chain reaction was used to identify the antimicrobial-resistant genes (blaZ, mecA, aacA-aphD, msrA, tetK, gyrA, grlA, and dfrG) in isolates of MRSA with a cefoxitin-resistant phenotype.

Results: From 218 specimens, 185 S. aureus were isolated, with the majority of these being found in the oral cavity (29.73%) and ventral thoracic area (22.7%), respectively. Forty-seven (25.41%) MRSAs were found in S. aureus isolates, with the majority of these being found in the perineum (16, 34.04%) and ventral thoracic area (13, 27.66%) specimens. Among MRSAs, 29 (61.7%) isolates were multidrug-resistant (MDR) strains. Most of MRSA isolates were resistant to penicillin (100%), followed by ceftriaxone (44.68%) and azithromycin (44.68%). In addition, these bacteria contained the most drug-resistance genes, blaZ (47.83%), followed by gyrA (36.17%) and tetK (23.4%).

Conclusion: This study revealed that MRSA could be found even in healthy rabbits. Some MRSAs strains were MDR–MRSA, which means that when an infection occurs, the available antibiotics were not effective in treating it. To prevent the spread of MDR–MRSA from pets to owners, it may be helpful to educate owners about effective prevention and hygiene measures.

Keywords: antimicrobial resistance, antimicrobial-resistant genes, healthy rabbits, methicillin-resistant Staphylococcus aureus.