Immunoglobulin subclass in experimental murine Toxocara cati infection

Research (Published online: 13-11-2017)

9. Immunoglobulin subclass in experimental murine Toxocara cati infection

Kusnoto, Setiawan Koesdarto, Sri Mumpuni and R. Heru Prasetyo

Veterinary World, 10(11): 1333-1336

ABSTRACT

Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.

Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.

Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.

Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Keywords: diagnostic kit, enzyme-linked immunosorbent assay, human, immunoglobulin G subclass, monoclonal antibody, toxocariasis, Toxocara cati.