Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail

Research (Published online: 23-12-2015)

12. Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail - Diwesh Kumar Niraj, Pushpendra Kumar, Chinmoy Mishra, Raj Narayan, Tarun Kumar Bhattacharya, Kush Shrivastava, Bharat Bhushan, Ashok Kumar Tiwari, Vishesh Saxena, Nihar Ranjan Sahoo and Deepak Sharma

Veterinary World, 8(12): 1435-1443

   doi: 10.14202/vetworld.2015.1435-1443

Abstract

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Aim: An attempt has been made to study the Myxovirus resistant (Mx1) gene polymorphism in Japanese quail.

Materials and Methods: In the present, investigation four fragments viz. Fragment I of 185 bp (Exon 3 region), Fragment II of 148 bp (Exon 5 region), Fragment III of 161 bp (Exon 7 region), and Fragment IV of 176 bp (Exon 13 region) of Mx1 gene were amplified and screened for polymorphism by polymerase chain reaction-single-strand conformation polymorphism technique in 170 Japanese quail birds.

Results: Out of the four fragments, one fragment (Fragment II) was found to be polymorphic. Remaining three fragments (Fragment I, III, and IV) were found to be monomorphic which was confirmed by custom sequencing. Overall nucleotide sequence analysis of Mx1gene of Japanese quail showed 100% homology with common quail and more than 80% homology with reported sequence of chicken breeds.

Conclusion: The Mx1 gene is mostly conserved in Japanese quail. There is an urgent need of comprehensive analysis of other regions ofMx1 gene along with its possible association with the traits of economic importance in Japanese quail.

Keywords: Japanese quail, Mx1 gene, nucleotide sequencing, polymorphism, polymerase chain reaction-single-strand conformation polymorphism.

Dominant viral pathologies in the extensive and semi-intensive animal breeding and their treatment mode in ethno veterinary medicine in Benin

Research (Published online: 23-12-2015)

11. Dominant viral pathologies in the extensive and semi-intensive animal breeding and their treatment mode in ethno veterinary medicine in Benin - T. M. Kpodékon, C. A. Ogni, H. Dassou, T. J. Dougnon, C. Boko, G. B. Koutinhouin, J. S. E. Goussanou, A. Akoegninou and I. Youssao

Veterinary World, 8(12): 1424-1434

   doi: 10.14202/vetworld.2015.1424-1434

Abstract

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Aim: This study aims to identify the dominant viral animal pathologies and to list the traditional recipes used by the breeders for their treatment.

Materials and Methods: The method of data collection was based on a retrospective survey. Thus, 787 breeders and agro-breeders scattered in the eight agro-ecological areas of Benin were interviewed using semi-structured questionnaires.

Results: In total, 5 pathologies were reported by breeders. Among those pathologies, foot and mouth disease was reported by all of the breeders of the southern part of Borgou compared with the other areas (p<0.05) and treated by 25 species of medicinal plants. African swine fever was the main pathology reported (22.92%) (p<0.05) in the fishery areas which is controlled by 7 medicinal plants. Pseudorinderpest was more reported (33.78%) (p<0.05) in the cotton area of central Benin and treated by 8 medicinal plants. There is also Newcastle disease that was mostly reported in the Western Atacora and treated by 32 medicinal plants as well as fowl pox which was a more reported in the lands of the bar area and the low-pressure area about 34.48% and 36.17% proportions, respectively, and treated by eight medicinal plants.

Conclusion: The breeders in Benin possess rich ethno veterinary knowledge on medicinal plants and their uses in the treatment of livestock. A total of 57 medicinal plants have been inventoried to fight against five major viral diseases as African swine fever, pseudorinderpest and foot and mouth disease. The common plants used to treat viral disease in general were Euphorbia unispina,Euphorbia poissonii, Lannea acida, and Mangifera indica. The most harvested organs on the plants reported in this survey were the barks, the leaves, and the whole plants. To better develop our indigenous resources, it would be important to expand this ethno-pharmacological investigation to other diseases category.

Keywords: Benin, ethnoveterinary survey, medicinal plants, viral diseases.

Molecular identification of Campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being

Research (Published online: 18-12-2015)

10. Molecular identification of Campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being - Sonuwara Begum, M. Sekar, L. Gunaseelan, Monica Gawande, G. Suganya, P. Annal Selva Malar and A.Karthikeyan

Veterinary World, 8(12): 1420-1423

   doi: 10.14202/vetworld.2015.1420-1423

Abstract

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Aim: Campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species.The aim of this study was to identify Campylobacter jejuni and Campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (PCR).

Methodology: A total of 104 number of samples comprising cloacal swab from poultry (38), a rectal swab from dogs (40), and calves (26) were collected for the isolation of thermophilic Campylobacters using conventional culture method. PCR was used for identification of mapA gene for C.jejuni and ceuE for C.coli.

Results: The overall presence of Campylobacter was found to be 67(64.42%) from the samples, out of which 6 isolates belongs to C. jejuni species, were 5(18.51%) from chicken and 1(4.17%) from dog was recorded and about 17 isolates belongs to C. coli species were 9(33.33%), 6 (25%), and 1(9.09%) from chicken, dog and calves was recorded.

Conclusion: Results suggested that Campylobacter reservoirs chicken, calves and pet dogs can play a role as the source of infection to human beings and PCR can be an ideal tool for molecular confirmation at the species level.

Keywords: emerging, pathogen, thermophilic Campylobacter, zoonotic. 

Seroepidemiology of infectious bovine rhinotracheitis infection in unvaccinated cattle

Research (Published online: 18-12-2015)

9. Seroepidemiology of infectious bovine rhinotracheitis infection in unvaccinated cattle - M. Saravanajayam, K. Kumanan and A. Balasubramaniam

Veterinary World, 8(12): 1416-1419

   doi: 10.14202/vetworld.2015.1416-1419

Abstract

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Aim: The present study aimed to investigate the seroepidemiology of infectious bovine rhinotracheitis (IBR) infection in the non-vaccinated cattle population in northern part of Tamil Nadu, India.

Materials and Methods: A total of 255 sera samples were collected from cattle having the history of respiratory and reproductive disorder from cattle of different age, breeds, and sex. All the sera samples were subjected to indirect ELISA for the diagnosis of IBR antibodies.

Results: Results revealed that the seroprevalence of IBR infection among non-vaccinated cattle population was of 65.88%. No significant difference was noticed in the prevalence of IBR infection between cattle showing respiratory (63.64%) and reproductive form (70.89%) (p≥0.05). A higher prevalence was noticed in animals above 3 years of age (59.60%) and in crossbred animals (71.26%) than young and non-descript animals. This study showed the higher prevalence of IBR infection in female (67.92%) than in male (33.33%).

Conclusion: Cattle population in this part can better be protected with vaccination than leaving them unvaccinated and seromonitoring shall have to be stressed with regular attempts to isolate and characterize the causative agent for IBR.

Keywords: cattle, enzyme-linked immunosorbent assay, infectious bovine rhinotracheitis, prevalence.

Feasibility of C-arm guided closed intramedullary pinning for the stabilization of canine long bone fractures

Research (Published online: 18-12-2015)

8. Feasibility of C-arm guided closed intramedullary pinning for the stabilization of canine long bone fractures - Anupreet Kaur, Ashwani Kumar, Deepesh Kumar, Jitender Mohindroo and Narinder Singh Saini

Veterinary World, 8(12): 1410-1415

   doi: 10.14202/vetworld.2015.1410-1415

Abstract

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Aim: To evaluate the feasibility of C-arm guided closed intramedullary pinning (simple Steinmann and end threaded) techniques for the stabilization of various canine long bone fractures.

Materials and Methods: The present study was conducted on 19 dogs with long bone fractures which were stabilized using simple Steinmann (Group I; n=6) and end threaded (Group II; n=13) pinning under C-arm guidance. Signalment, history of trauma, clinical examination, and hematobiochemical findings were recorded at the time of presentation. Radiography of the affected limb was carried out in two views to determine type and site of the fracture. Treatment of all the fractures was attempted using simple Steinman and end threaded pinning under the C-arm guidance. The success and failure of the closed technique were correlated with age, site, and type of fractures.

Results: The mean body weight and age of the dogs were 18.53±2.18 kg and 21.58±5.85 months, respectively. Early presented cases at a mean day of 2.84±0.54 were included. Out of 19 cases, it was possible to place implant successfully in 10 cases (success rate 52.63%) only. The remaining 9 cases had serious intraoperative complications like a misdirection of the pin after engaging the proximal fragment (n=3), missing the proximal fragment completely, and formation of the false tract (n=6). The majority of these complications were associated with younger age and proximal or distal third oblique fractures. High success rate of C-arm guided closed pinning was observed in midshaft fractures (75%) and transverse fractures (77.78%) in dogs of more than 1 year of age (77.78%). Simple Steinmann pinning was better feasible in a closed manner with a high success rate (66.70%) but also had implant related complications. Although, C-arm guided end threaded pinning was less (46.15%) successful, slightly tedious and time-consuming but had better implant stability than that of simple intramedullary pinning.

Conclusions: From the present study, it was concluded that C-arm guided closed pinning is recommended in transverse and midshaft fractures of the long bones in dogs older than 1 year of age. Furthermore, there is need to improve traction devices for enhancing the success of C-arm guided intramedullary pinning in dogs.

Keywords: C-arm guided pinning, closed pinning, dog, end threaded pin, fracture.

Study on prevalence of ancylostomosis in dogs at Anand district, Gujarat, India

Research (Published online: 17-12-2015)

7. Study on prevalence of ancylostomosis in dogs at Anand district, Gujarat, India - Nilima N. Brahmbhatt, P. V. Patel, Jigar J. Hasnani, Suchit S. Pandya and B. P. Joshi

Veterinary World, 8(12): 1405-1409

   doi: 10.14202/vetworld.2015.1405-1409

Abstract

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Aim: This study was undertaken to derive the prevalence rate of ancylostomosis in dogs by a collection of fecal samples from Anand district.

Materials and Methods: The fecal samples were collected from the dogs brought to the Hospital of Veterinary College (Teaching Veterinary Clinical Service Complex) and the surrounding areas of Anand district. On the day of collection, fecal samples were collected and brought to the Department of Veterinary Parasitology and processed for standard qualitative examination. The sedimentation technique was used to detect the presence of Ancylostoma spp. eggs in the samples.

Result: The highest prevalence rate was observed in the month of May (36.66% fecal samples) and the lowest in the month of December (13.79% fecal samples) at Anand district.

Conclusion: It can be concluded that heavy infection is present in Anand district especially in the season of summer followed by monsoon and the least in winter.

Keywords: ancylostomosis, dog, fecal, prevalence rate, sedimentation technique.

Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia

Research (Published online: 17-12-2015)

6. Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia - Sabry A. Hassan and Mohamed Y. Shobrak

Veterinary World, 8(12): 1400-1404

   doi: 10.14202/vetworld.2015.1400-1404

Abstract

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Aim: To determine the genetic basis and types of beta-lactamase encountered among enterobacterial isolates of wild pets from the animal exhibit.
Materials and Methods: A total of 17 beta-lactamase-producing enterobacteria recovered from fecal samples of wild pet animals were analyzed for a selected beta-lactamase gene by polymerase chain reaction.
Results: Molecular analysis identified one or more β-lactamase-encoding genes in 14 enterobacterial isolates as a single or gene combination. The most frequent extended-spectrum β-lactamases types were transmission electron microscopy and CTX-M, and the most common AmpC enzymes were CMY-2 and DHA types.
Conclusions: The study is the first in Saudi Arabia, have established the presence of β-lactamase-encoding genes in the fecal isolates of wild pets.
Keywords: animal exhibit, extended-spectrum β-lactamases/AmpC beta-lactamase, fecal samples, polymerase chain reaction, Saudi Arabia.