Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail

Research (Published online: 23-12-2015)

12. Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail - Diwesh Kumar Niraj, Pushpendra Kumar, Chinmoy Mishra, Raj Narayan, Tarun Kumar Bhattacharya, Kush Shrivastava, Bharat Bhushan, Ashok Kumar Tiwari, Vishesh Saxena, Nihar Ranjan Sahoo and Deepak Sharma

Veterinary World, 8(12): 1435-1443

   doi: 10.14202/vetworld.2015.1435-1443

Abstract

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Aim: An attempt has been made to study the Myxovirus resistant (Mx1) gene polymorphism in Japanese quail.

Materials and Methods: In the present, investigation four fragments viz. Fragment I of 185 bp (Exon 3 region), Fragment II of 148 bp (Exon 5 region), Fragment III of 161 bp (Exon 7 region), and Fragment IV of 176 bp (Exon 13 region) of Mx1 gene were amplified and screened for polymorphism by polymerase chain reaction-single-strand conformation polymorphism technique in 170 Japanese quail birds.

Results: Out of the four fragments, one fragment (Fragment II) was found to be polymorphic. Remaining three fragments (Fragment I, III, and IV) were found to be monomorphic which was confirmed by custom sequencing. Overall nucleotide sequence analysis of Mx1gene of Japanese quail showed 100% homology with common quail and more than 80% homology with reported sequence of chicken breeds.

Conclusion: The Mx1 gene is mostly conserved in Japanese quail. There is an urgent need of comprehensive analysis of other regions ofMx1 gene along with its possible association with the traits of economic importance in Japanese quail.

Keywords: Japanese quail, Mx1 gene, nucleotide sequencing, polymorphism, polymerase chain reaction-single-strand conformation polymorphism.