Friday, 10 July 2015

Hematocrit alterations and its effects in naturally infected indigenous cattle breeds due to Trypanosoma spp. on the Adamawa Plateau - Cameroon

Research (Published online: 30-06-2015)
22.  Hematocrit alterations and its effects in naturally infected indigenous cattle breeds due to Trypanosoma spp. on the Adamawa Plateau - Cameroon  - A. Mamoudou, V. K. Payne and S. L. Sevidzem
Veterinary World, 8(6): 813-818



   doi: 10.14202/vetworld.2015.813-818

Abstract
Aim: An experimental study was carried out on 148 naturally infected indigenous cattle breeds with either single or mix infections of various species of trypanosomes. The objectives of this study were to determine the species of trypanosomes, observe their hematopathological consequences on host-related risk factors and to determine the packed cell volume (PCV) of the infected group.
Materials and Methods: The buffy coat method (BCM) which is a variant of the hematocrit centrifugation method was used for the parasitological and hematological analysis. The May Grünwald-Giemsa method was also used for the identification of different trypanosome species.
Results: The infection rate in accordance with the various trypanosomes was as follows: Trypanosoma congolense + Trypanosoma brucei (1.35%), Trypanosoma vivax + T. brucei (1.35%), T. congolense + T. vivax (8.11%), T. congolense + T. vivax + T. brucei(8.78%), T. brucei brucei (11.48%), T. vivax (20.94%), T. congolense (47.97%). The infection rate with respect to breeds showed the following results - Brahman (1.0%), Red Fulani (5.2%), White Fulani (6.5%) and Gudali (16.7%), with no statistical significant difference (p>0.05). The combined mean PCV of single as well as mix infections was not statistically significant (p>0.05). The mean PCV of males (25.64±5.08 standard deviation [SD]) which was lower than that of females (30.82±4.94 SD) was statistically significant (p<0.05). The body condition of infected animals with sex showed that a greater proportion of males with "Poor" and "Medium" conditions showed high prevalence than females with the same conditions, with a significant difference (p<0.05). However, females showed a "Good" condition than males even though it was not statistically significant (p>0.05). The PCV profile of the infected group showed that the highest proportion of infected animals had PCV of ≤31% than PCV >31%. The mean weight of the animals was (265.41±95.36 SD). A scatter-linear plot of infected buffy coat against mean PCV showed a negative parametric correlation.
Conclusion: Distinguished Trypanosoma spp. pathogenicity, emaciation and weight loss related anemia, poor body condition, sex and the response of different breeds to various trypanosomes were highly affected and are of vital importance in diagnosis and act as a contribution to future control and treatment plans in this area.
Keywords: hematocrit, buffy coat method, body condition, anemia, trypanosome, breed, cattle, Adamaoua Plateau - Cameroon.
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Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls

Research (Published online: 30-06-2015)
21.  Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls- Akhil Patel, Atul Saxena, Dilip Kumar Swain, Dushyant Yadav, Sanjay Singh Yadav, Abhishek Kumar and Anuj Kumar
Veterinary World, 8(6): 808-812



   doi: 10.14202/vetworld.2015.808-812

Abstract
Aim: This study was aimed to see the beneficial effect of butylated hydroxytoluene (BHT) as a semen additive of Hariana bull semen.
Materials and Methods: The study was carried out in Hariana bulls. Twenty-four ejaculates from two bulls were used for this study. Each ejaculate was extended with standard glycerolated egg yolk tris extender and supplemented with BHT at two concentrations as 0.5 mM (T1) and 1.0 mM (T2). After dilution, equilibration and 24 h of cryopreservation, the samples were analyzed for progressive motility, sperm viability and membrane integrity.
Results: Progressive motility, sperm viability and sperm membrane integrity were significantly (p<0.05) increased in the samples fortified with BHT as compared to the control during the process of cryopreservation and thawing. The BHT concentration of 1 mM revealed better results as compared to 0.5 mM.
Conclusion: Addition of 1.0 mM BHT was found better in cryopreservation of Hariana bull semen compared to 0.5 mM BHT and control samples. The addition of BHT has improved the sperm quality by acting as an antioxidant thereby reducing the lipid peroxidation of the sperms.
Keywords: sperm, viability, butylated hydroxytoluene, membrane integrity, antioxidant, Hariana bull.
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A comparative therapeutic management of anoestrus in buffaloes using insulin and GnRH

Research (Published online: 30-06-2015)
20.  A comparative therapeutic management of anoestrus in buffaloes using insulin and GnRH - R. D. Purkayastha, S. N. Shukla, O. P. Shrivastava and P. R. Kumar
Veterinary World, 8(6): 804-807



   doi: 10.14202/vetworld.2015.804-807

Abstract
Aim: Anoestrus is one of the most common functional disorders of the reproductive cycle in buffaloes. In spite of technical advancement, there is no single cure for the management of anoestrus. Therefore, the aim of this study was to find out the efficacy of gonadotropic releasing hormone (GnRH) and metabolic hormone for the management of true anoestrus in buffaloes.
Materials and Methods: The experimental animals were selected on the basis of history, gyneco-clinical examinations and progesterone estimation. Deworming was done with Fenbendazole and thereafter mineral mixture was given @ 50 g per animal per day for 10 days in all the selected buffaloes before the start of treatment. The selected buffaloes were randomly divided into four groups (n=25). In Group I, buffaloes were administered 20 μg of buserelin intramuscularly. Buffaloes of Group II were administered long-acting insulin @ 0.25 IU/Kg body weight subcutaneously for 5 consecutive days. In Group III, buffaloes were treated with a combination of insulin and buserelin in the above-mentioned doses whereas buffaloes of Group IV were kept as untreated control.
Results: The higher oestrus induction (64% vs. 28%) was found in Group III and differed significantly (p<0.05) as compared to control group. The conception rate (69.23% vs. 66.66%) was also found higher in Group III but did not differ significantly among the treated groups. The mean time taken for the onset of oestrus was recorded significantly shorter in insulin (8.80±0.69) and GnRH (7.60±0.92 days) alone and as compared to other (Group III, 14.43±0.83 and Group IV, 20.57±1.69 days) groups.
Conclusion: The results of this study indicated better fertility response using Insulin plus Buserelin in true anoestrus buffaloes under field conditions.
Keywords: anoestrus, buffalo, buserelin, gonadotropic releasing hormone, insulin.
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Effect of preputial washing on bacterial load and preservability of semen in Murrah buffalo bulls

Research (Published online: 28-06-2015)
19.  Effect of preputial washing on bacterial load and preservability of semen in Murrah buffalo bulls - G. S. Meena, V. S. Raina, A. K. Gupta, T. K. Mohanty, M. Bhakat, M. Abdullah and R. Bishist
Veterinary World, 8(6): 798-803



   doi: 10.14202/vetworld.2015.798-803

Abstract
Aim: To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls
Materials and Methods: A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculates from same bull with preputial washing by infusing normal saline (0.85%), KMnO4 (0.02%) and savlon (2.0%) to first, second and third bull, respectively. The microbial load and semen quality were evaluated during different hours of storage at refrigerated temperature (0, 24 and 48 h) and after thrawing of cryopreserved (at −196°C) semen.
Results: The results of preservation of semen at refrigerated temperature showed that bacterial load was markedly lower in ejaculates of bulls subjected to preputial washing. Semen preserved at refrigerator temperature and cryopreserved, the effect of washing solution was significant for individual motility (IM), non-eosiniphilic count, hypo-osmotic swelling reactivity (HOST), total plate count (TPC) and acrosome integrity. KMnO4 was found to be the best in lowering bacterial load, sperm abnormalities and in improving semen quality such as motility, non-eosinophilic count, HOST and acrosome integrity even up to 48 h of preservation and cryopreserved semen. Effect of duration of preservation and stage of cryopreservation was also significant for IM, non-eosiniphilic count, HOST, sperm abnormalities and acrosome integrity.
Conclusion: Overall the results suggested that preputial washing with KMnO4 solution improved the semen quality and reduced microbial load of Murrah buffalo bull’s semen preserved at refrigerated temperature and cryopreservation.
Keywords: preputial washing, bacterial load, semen quality, preservability, Murrah buffalo bull.
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Friday, 3 July 2015

Effect of feeding sugarcane press mud on carcass traits and meat quality characteristics of lambs

Research (Published online: 28-06-2015)
18.  Effect of feeding sugarcane press mud on carcass traits and meat quality characteristics of lambs - Ranjan Kumar, Subodh Kumar Saha and Sanjod Kumar Mendiratta
Veterinary World, 8(6): 793-797



   doi: 10.14202/vetworld.2015.793-797

Abstract
Aim: To explore the possibilities of feeding unconventional agro-industrial byproduct for livestock production. Sugarcane press-mud (SPM), is a byproduct derived from sugarcane industry, which is rich in protein as well as minerals. The effects of dietary inclusion of SPM at different levels on the carcass characteristics of lambs were evaluated.
Materials and Methods: SPM was incorporated in concentrate mixture at different levels 0% (SP0 - concentrate mixture without SPM [Control diet]), 10% (SP10 - concentrate mixture containing 10% SPM) and 20% (SP20 - concentrate mixture containing 20% SPM). The concentrate mixtures were fed along with wheat straw for 180 days. At the end of the experimental period, six lambs per group were slaughtered to evaluate carcass and meat quality characteristics.
Results: No significant difference was observed in dressing percentage on pre-slaughter weight or empty body weight basis of lambs fed different levels of SPM incorporated diets. Likewise carcass weight, carcass length, and wholesale cuts appeared to have similar values among groups. The yield of visceral organs, chemical composition, and sensory attributes were not statistically affected by inclusion of SPM in the diets except juiciness of control group meat was significantly (p<0.05) higher than treatment group (SP20).
Conclusion: The SPM can be incorporated in the diet of lambs up to the level of 20% without affecting the carcass characteristics of lambs.
Keywords: carcass characteristics, lambs, sensory attributes, sugarcane press-mud.
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Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections

Research (Published online: 24-06-2015)
17.  Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections - Eric Lim Teik Chung, Faez Firdaus Jesse Abdullah, Lawan Adamu, Ali Dhiaa Marza, Hayder Hamzah Ibrahim, Mohd Zamri-Saad, Abdul Wahid Haron, Abdul Aziz Saharee, Mohd Azmi Mohd Lila, Abdul Rahman Omar, Md Zuki Abu Bakar and Mohd Jefri Norsidin
Veterinary World, 8(6): 783-792



   doi: 10.14202/vetworld.2015.783-792

Abstract
Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes.
Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 1012 colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively.
Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastrointestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups.
Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future.
Keywords: buffalo heifers, clinico-pathology, hematology and biochemistry responses, oral route, Pasteurella multocida Type B:2, subcutaneous route.
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Effect of infrared lamps to ameliorate cold stress in Vrindavani calves

Research (Published online: 24-06-2015)
16.  Effect of infrared lamps to ameliorate cold stress in Vrindavani calves - Showkat A. Bhat, Bharat Bhushan, Sajad A. Sheikh, T. Chandrasekar, Asu Singh Godara, Pranay Bharti and K. Puhle Japheth
Veterinary World, 8(6): 777-782



   doi: 10.14202/vetworld.2015.777-782

Abstract
Aim: This study was conducted to determine the effect of infrared lamps to ameliorate cold stress in Vrindavani (Holstein Friesian × Brown Swiss × Jersey × Hariana) calves.
Materials and Methods: For the present investigation, ten newborn Vrindavani calves were randomly divided into two groups (G1 and G2) of five each. The experiment was conducted from 2nd November to 8th February when the environmental temperature was at the lowest. The calves of G1 were provided with no additional protection while the calves of G2 were protected against the cold weather by providing heat using the infrared lamps. The body weight (kg) of the calves was recorded at weekly interval. The blood samples collected within 6 h of birth and then at fortnightly interval were analyzed for packed cell volume (PCV, %), hemoglobin (Hb, g/dl). Besides, the serum biochemical parameters, viz., Total serum protein (TSP, g/l), albumin (g/l), globulin (g/l), albumin globulin ratio (A:G) and important stress parameters, viz., triiodothyronine (T3, ng/ml), thyroxine (T4, ng/ml) and cortisol (ng/ml) were also estimated.
Results: The calves of G2 showed higher body weight gain as compared to G1. The differences were found to be highly significant (p<0.01). The calves in G1 showed comparatively higher values of PCV and Hb and the differences were found to be significant (p<0.05) on 45th day for PCV and highly significant (p<0.01) on 60th day for PCV and on 45th day for Hb. The values of TSP and albumin were comparatively higher in calves of G1 as compared to G2 and the differences were highly significant (p<0.01) on 45th day for both TSP and albumin and significant (p<0.05) on 60th day for albumin. Significantly (p<0.01) higher values of cortisol and T4 were observed on 15 and 45th day in calves of G1 as compared to G2. The T3 levels were also found higher in calves of G1 than G2 and the differences were significant (p<0.05) on 15 and 30th day and highly significant (p<0.01) on 45th day of the study.
Conclusion: Based on the results, it could be concluded that the infrared lamps are efficient in providing favorable microclimate and hence can be effectively used in calf shed to protect newborn calves from adverse conditions of winter and to improve their body growth performance.
Keywords: body weight, cold stress, infrared lamps, Vrindavani calves.
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