Antimicrobial resistance of commensal Escherichia coli from food-producing animals in Russia

Research (Published online: 02-10-2020)

3. Antimicrobial resistance of commensal Escherichia coli from food-producing animals in Russia

Dmitry A. Makarov, Olga E. Ivanova, Sergey Yu. Karabanov, Maria A. Gergel and Anastasia V. Pomazkova

Veterinary World, 13(10): 2053-2061

Background and Aim: Commensal Escherichia coli is an important indicator of antimicrobial resistance (AMR) in animals and food of animal origin. Therefore, it was recommended by the World Health Organization and OIE for inclusion in resistance surveillance programs. At the same time, the data on E. coli isolates from animals in Russia are scarce. The aim of this work was to determine the current prevalence of resistance and genetic markers of non-pathogenic commensal E. coli collected from major food-producing animals (poultry, pigs, and cows) in different regions of Russia and to compare these data with data from other countries to prioritize antimicrobials for limiting their use according to the National Action Plan.

Materials and Methods: Samples (n=306) were collected from biomaterial of chicken, turkey, cows, and pigs raised on 11 farms in the European part of Russia, Siberia, and North Caucasus. Isolates (n=306) of E. coli were tested for resistance to 11 antimicrobials from ten classes using the broth microdilution method. MICs were interpreted against EUCAST microbiological and clinical breakpoints. For data analysis and statistical processing, the AMRcloud online platform was used. The data are presented in comparison with other countries.

Results: In Russia, higher levels of microbiological and clinical resistance of E. coli to critically important antimicrobials, including colistin, cefotaxime, and ciprofloxacin, were found compared to other countries, especially in poultry: About 30% of isolates from chicken were resistant to colistin, 8% to cefotaxime, and 88% to ciprofloxacin according to EUCAST ECOFFs. Only 10% of isolates from cows were resistant to cefotaxime. About 47% of isolates of E. coli from chicken had a moderate relative resistance for ampicillin and 56% for tetracycline. For most antimicrobials, isolates from cows demonstrated a lower resistance than isolates from poultry and pigs. All tested isolates from chicken, turkey, and pigs showed a simultaneous microbiological resistance to at least three classes of antimicrobials. No pan-resistant isolates were found.

Conclusion: High levels of AMR of commensal E. coli from poultry, especially for critically important drugs, are a matter of concern and should be taken into account when choosing antimicrobials to be restricted for use in animal husbandry according to the National Action Plan.

Keywords: animals, antimicrobial resistance, commensal bacteria, critically important antimicrobials, Escherichia coli, multidrug resistance.

Effect of the bovine TG5 gene polymorphism on milk- and meat-producing ability

Research (Published online: 02-10-2020)

2. Effect of the bovine TG5 gene polymorphism on milk- and meat-producing ability

I. Dolmatova, T. Sedykh, F. Valitov, R. Gizatullin, D. Khaziev and A. Kharlamov

Veterinary World, 13(10): 2046-2052

Aim: This study aimed to determine the effect of thyroglobulin (TG5) gene polymorphism on milk and meat productivity in the various cattle breeds currently bred in the Republic of Bashkortostan.

Materials and Methods: The test was performed on dairy cattle of Black-and-White, Bestuzhev, and Simmental breeds, and meat cattle of Hereford and limousine breeds. The purpose of the test was to search for associations between the polymorphic alleles of the thyroglobulin (TG5) gene and economically useful traits.

Results: All studied breeds showed a frequency predominance of the TG5C allele (from 0.56 to 0.71). A clear trend of an effect of the genotypes of the TG5 gene on milk-productivity indicators was revealed; cows with the TG5TT genotype have the highest milk yield and fat content in milk. The milk of cows of Bestuzhev and Simmental breeds that possessed this genotype was also characterized by higher protein content.

Conclusion: We identified an effect of the polymorphism of the TG5 gene in the Hereford and limousine breeds on fat metabolism intensity indicators, such as fat output and fat content, in the longissimus muscle and in the general sample of ground beef.

Keywords: alleles, dairy and meat productivity, genetic polymorphism, Hereford breed, limousine breed, thyroglobulin gene.

Cost-effective horse breeding in the Republic of Bashkortostan, Russia

Research (Published online: 01-10-2020)

1. Cost-effective horse breeding in the Republic of Bashkortostan, Russia

Almir Askarov, Alfiya Kuznetsova, Rasul Gusmanov, Aigul Askarova and Vitaliy Kovshov

Veterinary World, 13(10): 2039-2045

ABSTRACT

Background and Aim: There is a lack of reliable data in agribusiness regarding the economic efficiency of horse breeding, and this limits its further development. The purpose of this study was to create rational parameters for the development of productive horse breeding as an effective agricultural business, in particular, in relation to farms.

Materials and Methods: The methods of investigation used were induction and deduction, as well as analytical, statistical, and economic-mathematical analysis. We also used the dynamics of time series, CVP analysis, direct costing, and microeconomic analysis. Data were taken from the Russian Federation's official statistics on animal husbandry as well as closed (commercial) data of agricultural enterprises from our study region.

Results: Horse ownership in the Republic of Bashkortostan is higher than in the rest of Russia with about 9% of the total number of horses in Russia. We found that landowners need one hectare of arable land to ensure profit and that the highest economic income occurs on farms specializing in kumis production. The production of kumis under intensive farming is less profitable than with free-range horses kept in pastures. Family farms need a large amount of arable land with natural foliage to balance space and profit.

Conclusion: Successful implementation of these parameters will make it possible to turn agriculture into successful horse breeding businesses. The expected volume of agricultural production may be approximately 9-11 thousand US dollars per employee.

Keywords: break-even volume, efficiency, marginal income, productive droving horse-breeding.

The frequency of virulent genes and antimicrobial resistance patterns of diarrheagenic Escherichia coli isolated from stools of children presenting with diarrhea in a tertiary hospital in Abakaliki, Nigeria

Research (Published online: 01-10-2020)

9. The frequency of virulent genes and antimicrobial resistance patterns of diarrheagenic Escherichia coli isolated from stools of children presenting with diarrhea in a tertiary hospital in Abakaliki, Nigeria

Ebuka Elijah David, Muhammad Arfat Yameen, Ikechuku Okorie Igwenyi, Arthur Chinedu Okafor, Uket Nta Obeten, Doris Olachi Obasi, Uchenna Regina Ezeilo and Chidinma Nnenne David

International Journal of One Health, 6(2): 147-152

ABSTRACT

Aim: This study was aimed to determine the virulent genes and antibiotic resistance patterns among circulating diarrheagenic Escherichia coli (DEC) pathotypes in a tertiary care health center in east of Nigeria.

Materials and Methods: Diarrheal stool samples were obtained from 80 children under 5 years and E. coli was isolated and identified using standard biochemical and molecular methods. Multiplex polymerase chain reaction (PCR) was used to detect eight virulent genes of DEC. Disk diffusion method was used to determine the antibiotic susceptibility of DEC.

Results: DEC infection was observed in 54 (68%) children among which ial gene for enteroinvasive E. coli (EIEC) (40% [n=22]) was commonly detected followed by eltA/eltB for enterotoxigenic E. coli (ETEC) (30% [n=16]), pCVD for enteroaggregative E. coli (EAEC) (20% [n=11]), and eaeA/bfpA for typical enteropathogenic E. coli (EPEC) (10% [n=5]). The DEC isolates phenotypically exhibited resistance for ampicillin (AMP) (44 [81%]), followed by ciprofloxacin (CIP)/ levofloxacin (LEV) (28 [52%]), cefoxitin (FOX) (11 [20%]), and amoxicillin-clavulanic acid (AMC) (6 [11%]). About 60% isolates of stable toxins-ETEC were resistant to AMC, CIP, and LEV while all the labile toxin-ETEC exhibited resistance to AMP. About 60% (n=6) resistance were seen in EAEC against ampicillin, AMC, FOX, CIP, and LEV. In EIEC, all the isolates (n=22) were resistant to AMP while 50% (n=11) were resistant to both CIP and LEV. All EPEC (n=5) were resistant to AMP, FOX, CIP, and LEV.

Conclusion: High frequency of virulent ial and eltA/eltB genes for EIEC and ETEC, respectively, suggests that they are the primary etiological agents of diarrhea in children among DEC pathotypes. Resistance of DEC to more than two classes of antibiotics indicate possible emergence of multidrug resistance.

Keywords: antibiotic resistance, diarrheagenic Escherichia coli, diarrheal stool, multidrug resistance, multiplex polymerase chain reaction, polymerase chain reaction.

Hematology and serum biochemistry of free-range brown-throated sloths in two urban areas in Brazil

Research (Published online: 30-09-2020)

40. Hematology and serum biochemistry of free-range brown-throated sloths in two urban areas in Brazil

Kissia Ferreira Pereira, Ita de Oliveira e Silva, Fernanda de Fátima Rodrigues Silva, Vinicius Herold Dornelas e Silva, Carla Soraia Soares de Castro and Vanner Boere

Veterinary World, 13(9): 2033-2038

ABSTRACT

Background and Aim: The brown-throated sloth (Bradypus variegatus) is widely distributed in three biomes: The Amazon, Atlantic Forest, and Caatinga. Some subpopulations are isolated in urban areas in Brazil, usually in squares and small woods. Due to the scarcity of reference values, an investigation was carried out on the hematology and blood biochemistry of brown-throated sloths from urban areas.

Materials and Methods: Blood was collected by venipuncture in the femoral vein from 19 brown-throated sloths for hematological and biochemical analyses, living in two municipalities; Teófilo Otoni (TO) (Minas Gerais State) and Rio Tinto (RT) (Paraíba state), in the Atlantic Forest biome, Brazil. The samples were analyzed in specialized veterinary laboratories using automatic cell counters and slide fixation staining methods.

Results: The two subpopulations of brown-throated sloths had no significant differences in most hematological values, with the exception of a higher leukocyte concentration (p<0.01) in the RT sub-population. The difference in leukocyte concentration suggests an idiosyncratic effect, as the animals were healthy and the stress of the capture was acute, not chronic. The concentrations of urea, creatinine, and alkaline phosphatase (ALP) were similar (p<0.05) in brown-throated sloths from TO and RT. Likewise, the liver enzyme concentrations (ALP, alanine transaminase [ALT], and aspartate transaminase [AST]) did not differ between the two subpopulations (p<0.05).

Conclusion: Compared to another study, brown-throated sloths from TO and RT have higher plasma concentrations of ALT and ALP, suggestive of a hepatic overload. Hematological and blood biochemical findings of TO and RT can be used as clinical reference values for brown-throated sloths living in an urban environment.

Keywords: order pilosa, physiology, public square, urban fauna, wildlife.

Ocimum sanctum Linn. ethanolic extract inhibits angiogenesis in human lung adenocarcinoma (a549) cells

Research (Published online: 30-09-2020)

39. Ocimum sanctum Linn. ethanolic extract inhibits angiogenesis in human lung adenocarcinoma (a549) cells

Hevi Wihadmadyatami, Puspa Hening, Ulayatul Kustiati, Dwi Liliek Kusindarta, Teguh Triyono and Supriatno Supriatno

Veterinary World, 13(9): 2028-2032

ABSTRACT

Background and Aim: Ocimum sanctum (OS) is a herbal plant, which is easy to find and is widely used as an alternative medication. The previous studies have shown that several species of OS extract have therapeutic properties, and in some cases, antitumor properties. Furthermore, several data have shown the antiproliferative effects of OS extract in cases of breast cancer, human fibrosarcoma, and oral cancer. Lung adenocarcinoma is a major cause of male cancer worldwide; however, the effect of OS (of Indonesian origin) on the metastasis of human alveolar pulmonary adenocarcinoma A549 cells remains unclear. This study aimed to analyze the antiangiogenic effects of OS ethanolic extract in A549 lung adenocarcinoma cells.

Materials and Methods: An angiogenesis assay was performed by seeding A549 cells on extracellular matrix solution and observing tube formation using an inverted microscope. Enzyme-linked immunosorbent assay for αvβ3, matrix metalloproteinase (MMP)-2, and MMP-9 was performed by analyzing the cell lysate after a given treatment.

Results: OS ethanolic extract significantly inhibited tube formation of A549 cells and suppressed the expression of integrin αvβ3, MMP-2, and MMP-9.

Conclusion: Our findings indicate that OS ethanolic extract disrupts angiogenesis of A549 cells, which may result from the disruption of cell migration and proliferation as a consequence of downregulation of αvβ3, MMP-2, and MMP-9. Taken together, OS ethanolic extract may represent a good therapeutic candidate for the treatment of metastasis in lung adenocarcinoma. Further studies are warranted to further establish the efficacy of OS in the treatment of lung adenocarcinoma.

Keywords: A549 cells, angiogenesis, integrin αvβ3, matrix metalloproteinase, Ocimum sanctum ethanolic extract.

The prevalence and risk factors associated with Leptospira in donkeys in Ngaka Modiri Molema District, North West Province, South Africa

Research (Published online: 28-09-2020)

38. The prevalence and risk factors associated with Leptospira in donkeys in Ngaka Modiri Molema District, North West Province, South Africa

Kibambe Kiayima Daddy, Mulunda Mwanza, James Wabwire Oguttu and Lubanza Ngoma

Veterinary World, 13(9): 2020-2027

ABSTRACT

Background and Aim: Leptospirosis is one of the major emerging global economic and health problems affecting donkeys, thereby reducing their work output. Furthermore, the disease has public health importance because of its zoonotic nature. Despite the significant contribution donkeys make to the national economy, less attention is given to diseases that afflict donkeys and reduce their productivity and performance. A cross-sectional study was conducted to investigate the seroprevalence of Leptospira and identify risk factors associated with the occurrence of the disease among donkeys in the study area.

Materials and Methods: A questionnaire survey was used to collect the following data: Demographic, environmental, management, and health-related factors. Blood samples were aseptically collected from 365 randomly selected donkeys from 19 villages. The sera were tested using the microscopic agglutination test. Categorical variables were summarized and presented as proportions and their 95% confidence interval (CI). A binary logistic regression model was fitted to the data to identify risk factors associated with Leptospira seroprevalence in donkeys within the study areas.

Results: The majority of the donkeys (29.6%; n=108/365) were from Mafikeng local municipality, and the rest (19.7%; n=72/365) were from Ratlou. Just over half (58.1%; n=212/365) of the donkeys tested were female, and the remaining (41.9%; n=153/365) were males. In addition, most donkeys (42.7%; n=156/365) were between 6 and 12 years old, followed by those between 0 and 5 years (37%; n=135/365), and only 20.3% (n=74/365) were above 12 years. Out of the donkeys tested, 11.5% (95% CI: 4.86-18.14) donkeys tested positive for Leptospira antibodies. The most common serovar was Bratislava (81%; n=34/42), followed by Tarassovi (19.04%; n=8/42). While gender was positively associated with seroprevalence of the disease (Adjusted Odds Ratio [AOR]=4.88; p=0.0001), the presence of horses (AOR=0.226; p=0.002) and agricultural activities (AOR=0.093; p=0.0001) in the vicinity of the dwellings of the donkeys were negatively associated with Leptospira seropositivity in the study area.

Conclusion: Findings reported here show that donkeys in the study area are reservoirs for the predominant serovar Bratislava and the less dominant serovar Tarassovi. The gender of the donkey was a risk factor for Leptospira seroprevalence. Further studies are needed to investigate the role of agricultural activities in the vicinity of the dwellings of donkeys on the occurrence of Leptospira in the study area.

Keywords: donkeys, leptospirosis, risk factors, seroprevalence.