Tuesday, 25 September 2018

First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows

Research (Published online: 25-09-2018)
20. First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows
Meryem Guessoum, Zehor Guechi and Mounir Adnane
Veterinary World, 11(9): 1326-1330
ABSTRACT
Aim: The present study was conducted to detect and identify Helicobacter pylori within local cow breeds in the central region of Algeria.
Materials and Methods: Two hundred (n=200) cows from three provinces of the central region of Algeria were studied, between January 2016 and September 2017. Each cow was subject to stool, milk, and blood sampling. Milk and fecal samples were used to detect and identify H. pylori using bacteriology culture method. Blood and milk samples were used to detect H. pylori immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay. Polymerase chain reaction was used to confirm the abundance of H. pylori in milk by detecting glmM gene.
Results: Out of 200 sera and 200 milk samples, 12% (24) and 4% (8/200) were positive for the H. pylori IgG antibody. glmM gene was detected in the milk of 13% of cows and was confirmed in all cows presenting IgG in milk.
Conclusion: From the present study, we concluded that the glmM gene is an important marker for detecting H. pylori in milk. Moreover, Algerian local-breed cows are a source of H. pylori and could be responsible for serious zoonosis.
Keywords: Algeria, cows, feces, Helicobacter pylori, milk, serum.

Monday, 24 September 2018

Prevalence of antibodies against visceralizing Leishmania spp. in brown rats from Grenada, West Indies

Research (Published online: 24-09-2018)
19. Prevalence of antibodies against visceralizing Leishmania spp. in brown rats from Grenada, West Indies
Alexa Rosypal von Dohlen, Nautica Cheathem, Keshaw Tiwari and Ravindra Nath Sharma
Veterinary World, 11(9): 1321-1325
ABSTRACT
Background and Aim: Leishmania spp. are known to cause disease in man and animals. Rats are considered important reservoir hosts and transmission takes place through the bite of female sand fly, Phlebotomusspp. To the best of our knowledge, there is no published information on Leishmania infection in rats in Grenada. This study was conducted to estimate the antibodies for visceralizing Leishmania spp. (VL) in rats (Rattus norvegicus) from Grenada.
Materials and Methods: A total of 146 brown rats (R. norvegicus) were trapped live from two parishes (St. George and St. David) in Grenada. Following anesthesia, blood was collected from the heart through thoracic puncture. The serum was collected after the centrifugation of blood. Serum was tested for antibodies to VL. with a commercially available immunochromatographic dipstick test which is licensed for use in animals and humans.
Results: The seroprevalence of antibodies against Leishmania spp. was found in 34 of 146 rats (23.3%; CI 95% from 16.70 to 30.99). No significant differences were found between sexes and young or adults. The prevalence between parishes (St. George and St. David) was also not significant.
Conclusion: The results show that rats (R. norvegicus) in Grenada are exposed to Leishmania spp. The rats could play an important role in the transmission of leishmaniasis to humans and other animals in Grenada.
Keywords: brown rats, Grenada, leishmaniasis, prevalence, serum antibodies.

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Research (Published online: 24-09-2018)
18. Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes
Dahliatul Qosimah, Sri Murwani, Edhy Sudjarwo and M. Arfan Lesmana
Veterinary World, 11(9): 1316-1320
ABSTRACT
Background and Aim: Newcastle disease virus (NDV) is an obligate intracellular parasite. Virus can only live on living cells. The embryonated chicken eggs (ECEs) are one of the growth media of virus that is a cheap, easy to do, and accurate for showing patterns of virus change in the host. Higher virus titers indicate the higher number of viruses and more virulent to infect host. This research aimed to investigate the effect of different level of NDV titer infection in ECEs on protein profile, embryonic length, mortality, and pathological change.
Materials and Methods: The study used a completely randomized design of six treatments and seven replications. The treatments were different level of NDV titer infection in allantoic fluid (AF) of 9-11 days ECEs, i.e., P1=20, P2=26, P3=27, P4=28, P5=29, and P6=210 hemagglutination unit (HAU). All samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Data were analyzed using one-way ANOVA with p=0.05 for length of the embryo and descriptive analysis for embryo mortality, pathology change, and protein band.
Results: The result showed that protein profile of NDV-infected ECEs of all different levels is more complex than protein profile of no NDV-infected ECEs. NDV infected of all different levels showed longer size embryo, higher mortality embryo at the first 2 days, and higher occurrence of hemorrhagic in all part of bodies of embryo than those of no NDV infected.
Conclusion: It was concluded that NDV infection of all different level decreased health conditions of chicken embryo of ECEs of 9-11 days old. Different level of NDV infection of ECEs of 9-11 days old showed no significantly different embryo profiles. However, all of the NDV-infected embryos were shorter, death on the 2nd day, and suffered more hemorrhage on all body surfaces than uninfected NDV embryos.
Keywords: embryo, Newcastle disease, pathological change, protein, titer, virus.

Saturday, 22 September 2018

Prevalence and risk factors associated with brucellosis among high-risk individuals in Lafia, Nasarawa state, Nigeria

Research (Published online: 23-09-2018)
8. Prevalence and risk factors associated with brucellosis among high-risk individuals in Lafia, Nasarawa state, Nigeria
Charity Ashe'osla Agada, Jibrin Mohammed, Anthony Ekele Joseph Okoh and Joseph Akwoba Ogugua
International Journal of One Health, 4: 45-51
ABSTRACT
Background: Brucellosis caused by Brucella species is a zoonotic disease prevalent in Nigeria. Data on this disease in humans and its risk factors in Lafia, Nasarawa State, are lacking.
Materials and Methods: This study was conducted on high-risk individuals in the area using the Rose Bengal test (RBT) and questionnaire.
Results: Out of the 160 respondents tested for seropositivity to brucellosis, 10% (16/160) prevalence was recorded with the RBT. None of the factors evaluated was found to be significantly associated with the seroprevalence of brucellosis in the area. On the other hand, final logistic regression analysis found the knowledge of brucellosis to be significantly associated with the profession (OR=11.52; 95% CI=1.47-90.46; p=0.02) and age (OR=2.6; 95% CI=1.11-4.19; p=0.023). Furthermore, practices that expose to Brucella infection were found to be significantly associated with the site (OR=4.02; 95% CI=5.29- 333.33; p=0.000) and marital status (OR=2.44; 95% CI=1.03-5.75; p=0.042).
Conclusion: Brucellosis is prevalent among occupationally exposed individuals in Lafia. There should be continued surveillance for the disease and education of high-risk individuals in the area.
Keywords: age, brucellosis, humans, Lafia, prevalence, profession.

Thursday, 20 September 2018

Ruminal impaction due to plastic materials - An increasing threat to ruminants and its impact on human health in developing countries

Review (Published online: 20-09-2018)
17. Ruminal impaction due to plastic materials - An increasing threat to ruminants and its impact on human health in developing countries
M. Priyanka and S. Dey
Veterinary World, 11(9): 1307-1315
ABSTRACT
Ruminal impaction due to plastic materials is a condition, in which indigestible plastic foreign bodies accumulate in the rumen of ruminants leading to ruminal impaction, indigestion, recurrent tympany, and many other adverse health effects. It is caused by the indiscriminate feeding of ruminants on indigestible plastic waste materials. The disease is primarily noticed in stray animals residing in urban areas of developing countries. Ingested plastic materials in the rumen slowly release the chemicals in rumen fluid, which intern enter the food chain through milk and meat products. These chemicals have a detrimental effect on human health. At present, exploratory rumenotomy is the only choice for both diagnosis and treatment of ruminal impaction due to plastic materials in ruminants. Control measures include good animal husbandry practices and proper disposal of plastic waste materials. The present review discusses in depth about the epidemiology, pathophysiology, diagnosis, treatment, prevention, and control of ruminal impaction due to plastic materials in ruminants and also highlights its impact on human health.
Keywords: developing countries, human health, plastic materials, ruminal impaction, ruminants, urban areas.

Wednesday, 19 September 2018

The prevalence and intensity of external and internal parasites in working donkeys (Equus asinus) in Egypt

Research (Published online: 19-09-2018)
16. The prevalence and intensity of external and internal parasites in working donkeys (Equus asinus) in Egypt
Marwa M. Attia, Marwa M. Khalifa and Marwa Th. Atwa
Veterinary World, 11(9): 1298-1306
ABSTRACT
Aim: This study aims to record and update the prevalence and intensity of external and internal parasites in working donkeys (Equus asinus) in Egypt during the period from January to December 2017.
Materials and Methods: A total of 120 donkeys (10 donkeys each month) were examined at Giza zoo abattoir through bimonthly visits. The examined donkeys were obtained from five governorates (Giza [20], Fayoum [40], Beni Suef [30], Monofia [20], and Assiut [10]). The animals were grouped according to age and sex.
Results: All examined donkeys were positive with at least one internal or even external parasitic species. The overall prevalence rate was 100%. A total of 11 helminths species (10 nematodes and 1 metacestode); 7 protozoal and 7 arthropod species were collected. The number of each parasite and intensity of infection with regard to age and sex was recorded.
Conclusion: All examined donkeys were infected with parasites with an overall prevalence of 100%. So, we recommended following up and continuous treatment of such diseased animal.
Keywords: arthropods, donkeys, Egypt, external parasites, helminths, internal parasites, protozoa.

Tuesday, 18 September 2018

Acute oral toxicity of pesticide combination (acephate 50% and imidacloprid 1.8% as active ingredients) in Sprague-Dawley rats

Research (Published online: 18-09-2018)
15. Acute oral toxicity of pesticide combination (acephate 50% and imidacloprid 1.8% as active ingredients) in Sprague-Dawley rats
Rajendra Palkhade, Suresh Yadav, SukhDev Mishra and Jaseer Muhamed
Veterinary World, 11(9): 1291-1297
ABSTRACT
Aim: The aim of this study was to assess the acute toxic interaction and lethal dose (LD50) of pesticide combination product (acephate 50% and imidacloprid 1.8% as active ingredients) available in the market in Sprague-Dawley female rats by oral route.
Materials and Methods: A total of 10 Sprague-Dawley female rats were divided into two groups, comprising five rats in each dose group. Both groups were identified as control and test groups, respectively. Control group received sterile water as vehicle and test group received pesticide combination (acephate 50% and imidacloprid 1.8% as active ingredients) at a dose of 0 and 2000 mg/kg body weight. As per the Organization for Economic Cooperation and Development Guideline 420, initially one animal each from both the control and test groups were dosed with 0 and 2000 mg/kg, respectively, as sighting study. Based on the results of sighting study, additionally, four animals each from both groups were dosed with the same dose to make a total of five animals in each group. Dose volume was constant as 10 mL/kg. All animals were observed daily twice for clinical signs and mortality. Body weight was recorded on day 0 and weekly thereafter during 14 days' observation period; last body weight (fasted) was recorded on day 15. All the rats of both the groups were humanely sacrificed on day 15 for gross pathology, collection of organs for histopathology, organ weighing, and morphometry. Organ weights were taken as absolute values, and relative organ weights to last fasted body weights were calculated.
Results: Pesticide combination (acephate 50% and imidacloprid 1.8% as active ingredients) treated rats showed cholinergic signs with one mortality in the test group. No significant difference was observed in body weight, relative organ weights, and organ morphometry between pesticide combination exposed and non-exposed groups. Gross pathology of the treated rats was also comparable with respect to control group. Histopathological changes in the liver, kidneys, heart, lung, adrenaline, spleen, and ovaries of test group rats were found to be comparable with control group rats.
Conclusion: The present study demonstrated the LD50 of one of the combination products available in the market having acephate 50% and imidacloprid 1.8% as active ingredients in Sprague-Dawley female rats which is >2000 mg/kg body weight. Furthermore, gross, histopathology and histoarchitectural alterations of all the vital organs of the test group were comparable to the control.
Keywords: acephate 50%, acute oral toxicity, histopathology, imidacloprid 1.8%.