Thursday, 8 June 2017
Using real-time polymerase chain reaction as an alternative rapid method for enumeration of colony count in live Brucella vaccines
Tuesday, 6 June 2017
Cytological endometritis and its agreement with ultrasound examination in postpartum beef cows
doi: 10.14202/vetworld.2017.605-609
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Article history: Received: 18-02-2017, Accepted: 13-04-2017, Published online: 07-06-2017
Corresponding author: N. Yimer
E-mail: nurdeg2006@gmail.com
Citation: Salah N, Yimer N (2017) Cytological endometritis and its agreement with ultrasound examination in postpartum beef cows, Veterinary World, 10(6): 605-609.Background: Endometritis, which is one of the most common diseases in dairy cows postpartum, causes severe economic losses, including increased open days, calving intervals, and numbers of services to achieve conception.
Aim: This study aimed to evaluate the ultrasound method and its agreement with the endometrium cytology method, which is used to diagnose cytological endometritis in beef cows. Moreover, we determined which method has higher sensitivity and specificity at 4 and 5 weeks postpartum.
Materials and Methods: The study was conducted 20-35 days postpartum. A total of 53 clinically healthy beef cows (28 Brangus and 25 Kedah-Kelantan breeds) from three beef farms were obtained. All cows were evaluated at 4 and 5 weeks postpartum, using ultrasound and cytobrush endometrial examination methods to diagnose cytological endometritis.
Results: Endometrial cytology result showed that 11.3% (6/53) and 9.4% (5/53) of the cows exhibited cytological endometritis 4 and 5 weeks postpartum, respectively. A weak-to-moderate agreement found between the diagnostic methods (k=0.29 - 0.50; p<0.01 and k=0.38 - 0.49) at 4 and 5 weeks postpartum respectively.
Conclusion: The percentage of beef cows that were positive to cytological endometritis was low (polymorphonuclear cells, =8%) at 4 and 5 weeks postpartum. Results showed that the ultrasound method is useful and practical for diagnosing endometritis 4 and 5 weeks postpartum. This method exhibited 60% sensitivity, 93.8% specificity, and a 0.50 kappa value, especially when presence of intrauterine fluids and measurement of cervix diameter used in combination.
Keywords: beef cows, cytology, endometritis, polymorphonuclear cells, ultrasound.
Characterization of hemagglutination activity of emerging Newcastle disease virus in Bangladesh
Keywords: chilling temperature, hemagglutination, incubation temperature, Newcastle disease virus, Newcastle disease virus lasota strain, very virulent Newcastle disease virus strain.
Monday, 5 June 2017
Molecular characterization of antibiotic-resistant Staphylococcus aureus from livestock (bovine and swine)
Aim: The aim of this study was to figure the prevalence, phenotypic and genotypic antibiotic resistance (AR) pattern of Staphylococcus aureus isolated from bovine and swine nares.
Sunday, 4 June 2017
Prevalence of methicillin-resistant Staphylococcus aureus skin and nasal carriage isolates from bovines and its antibiogram
isolates from bovines and its antibiogram
Staphylococcus aureus (MRSA) in cattle and buffalo and to study their antibiotic
resistance pattern.
were collected. MRSA was identified by conventional bacterial culture techniques which were
further confirmed by amplification of S. aureus-specific 16S rRNA by polymerase chain reaction
(PCR). The isolates were further analyzed for the presence of mecA gene by PCR. The
antimicrobial susceptibility profiling was performed by disc diffusion method.
and skin swab, respectively, with an overall prevalence of 31.43% MRSA among cattle. Buffalo
nasal and skin sample showed MRSA prevalence of 54.55% and 39.4%, respectively, with 46.9%
overall prevalence. PCR could detect mecA gene in 36.4% and 58% MRSA isolates from cattle
and buffalo, respectively. Antimicrobial susceptibility test found MRSA resistant to penicillin and
oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole (62%), and amoxyclav (50%).
100% sensitivity was observed against ciprofloxacin, amikacin, chloramphenicol, and gentamicin.
Three (16.7%) MRSA isolates from buffalo were found resistant to vancomycin.
emergence of VRSA in animal population which may be transmitted to the human beings
working in close contact to the animals.