Effectiveness of a recombinant human follicle stimulating hormone on the ovarian follicles, peripheral progesterone, estradiol-17β, and pregnancy rate of dairy cows

Research (Published online: 05-07-2016)

4. Effectiveness of a recombinant human follicle stimulating hormone on the ovarian follicles, peripheral progesterone, estradiol-17β, and pregnancy rate of dairy cows - Mohamed Ali and Moustafa M. Zeitoun

Veterinary World, 9(7): 699-704

   doi: 10.14202/vetworld.2016.699-704

Abstract

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Aims: This study aimed at elucidating the effects of recombinant human follicle stimulating hormone (r-hFSH) on the ovarian follicular dynamics, progesterone, estradiol-17β profiles, and pregnancy of dairy cows.

Materials and Methods: Three groups (G, n=5 cows) of multiparous dairy cows were used. G1 (C) control cows were given controlled internal drug release (CIDR) and prostaglandin F2α; G2 (L) cows were given low dose (525 IU and G3 (H) cows were given high dose (1800 IU) of r-hFSH on twice daily basis at the last 3 days before CIDR removal. All cows were ultrasonically scanned for follicular growth and dynamics, and blood samples were collected every other day for two consecutive estrus cycles for the determination of estradiol-17β and progesterone.

Results: Estrus was observed in all C and L but not in H cows. Dominant follicle was bigger in L compared to C and H cows. Dominant follicle in C (16.00±2.5 mm) and L cows (17.40±2.3 mm) disappeared at 72 h after CIDR removal. However, in H cows, no ovulation has occurred during 7 days post-CIDR removal. Progesterone was not different (p>0.10) among groups, whereas estradiol-17β revealed significant (p<0.01) reduction in H (15.96±2.5 pg/ml) cows compared to C (112.26±26.1 pg/ml) and L (97.49±15.9 pg/ml) cows. Pregnancy rate was higher in L cows (60%) compared with C cows (20%). However, H cows were not artificially inseminated due to non-ovulation. Only a cow of C group has calved one calf, however, 2 of the L cows gave birth of twins and a cow gave single calf.

Conclusion: Administration of a low dose (525 IU) of r-hFSH resulted in an optimal size of dominant follicle, normal values of progesterone and estradiol-17β, and 40% twinning rate, howeverusing 1800 IU of r-hFSH, have adverse effects on ovarian follicular dynamics and hormonal profiles with non-pregnancy of dairy cows raised under hot climate.

Keywords: dairy cows, estradiol-17β, follicles, progesterone, recombinant human follicle stimulating hormone.

Diagnosis of pathological conditions of kidney by two-dimensional and three-dimensional ultrasonographic imaging in dogs

Research (Published online: 04-07-2016)

3. Diagnosis of pathological conditions of kidney by two-dimensional and three-dimensional ultrasonographic imaging in dogs - Dinesh Dehmiwal, S. M. Behl, Prem Singh, Rishi Tayal, Madan Pal and R. K. Chandolia

Veterinary World, 9(7): 693-698

   doi: 10.14202/vetworld.2016.693-698

Abstract

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Aim: The objective of the study was to obtain and compare two-dimensional (2D) and three-dimensional (3D) ultrasonographic images of the kidney in different disease conditions.

Materials and Methods: In this study, 11 clinical cases of different age groups of dogs suffering from kidney diseases were diagnosed by 2D and 3D ultrasonography at Teaching Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar. The ultrasound (US) machine used for this study was 3D US machine (Nemio-XG: Toshiba, Japan) having four-dimensional (4D) volumetric probe. The images were acquired with 3-6 MHz 2D curvilinear transducer and 4.2-6 MHz 4D volumetric curvilinear transducer.

Results: Nephritis was diagnosed in four dogs aged between 5 months and 6 years. In all the cases of nephritis diffuse increase in echogenicity of kidney, parenchyma was observed. Two dogs with end-stage kidney disease were also diagnosed. In both 2D and 3D ultrasonography, the kidney size was decreased and architectural details were also lost in them. The cases of regional renal diseases diagnosed were hydronephrosis and nephrolithiasis. Dilated renal pelvis was the common finding in all the three cases of hydronephrosis in both 2D and 3D ultrasonogram. Nephroliths were observed in one case with the history of hematuria and oliguria. The multifocal renal disease diagnosed in this study was dysplastic polycystic kidney. In 2D ultrasonogram, six anechoic cavities appeared with thin strip of renal parenchyma. In 3D ultrasonogram, the cysts appeared as black anechoic areas.

Conclusion: The result of the current study showed that the clinical conditions of kidney such as nephritis, end-stage kidney, hydronephrosis, polycystic kidney, and nephrolithiasis can be diagnosed easily using 2D and 3D ultrasonography. Visualization of renal structures was clear in 2D ultrasonography in the conditions of nephritis and end-stage kidney. However, the conditions such as hydronephrosis, polycystic kidney, and nephrolithiasis were visualized clearly in both 2D and 3D ultrasonography equally.

Keywords: hydronephrosis, hyperechoic, hypoechoic, nephritis, ultrasonography.

Evaluation of edible polymer coatings enriched with green tea extract on quality of chicken nuggets

Research (Published online: 02-07-2016)

2. Evaluation of edible polymer coatings enriched with green tea extract on quality of chicken nuggets - Prathyusha Kristam, Naga Mallika Eswarapragada, Eswara Rao Bandi and Srinivas Rao Tumati

Veterinary World, 9(7): 685-692

   doi: 10.14202/vetworld.2016.685-692

Abstract

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Aim: The present study was conducted to evaluate the physico-chemical and microbiological characteristics of chicken nuggets coated with sodium alginate (SA) coatings at refrigerated (4±1°C) and frozen (−18±1°C) storage condition at regular periodic intervals.

Materials and Methods: Chicken meat nuggets were separated into three groups: Uncoated control (C), coated with alginate coating (T₁), and coated with alginate coating incorporated with 1% green tea extract (GTE) (T₂). The nuggets were analyzed at regular intervals of 5days for refrigerated storage and 15 days for frozen storage period in terms of pH, 2-thiobarbituric acid value (TBA), peroxide value (PV), total plate count (TPC), water loss, and sensory characteristics.

Results: The results indicated that the nuggets coated with alginate-based coatings effectively reduced the spoilage as indicated by pH, TBA, and PVs. pH values of the formulations ranged from 6.15 to 6.34 at refrigerated storage temperature (4±1°C) and 6.49-6.71 at frozen storage temperature (−18±1°C). TBA value of the treatments ranged from 1.28 to 1.54 mg MDA/kg and 1.34 to 1.50 mg MDA/kg under refrigerated and frozen storage temperatures, respectively. Color, flavor, juiciness, tenderness, and overall acceptability of the nuggets differed significantly (p<0.05) with the coated nuggets. The coated nuggets were well acceptable upto 15 days at refrigerated storage temperature (4±1°C) and upto 75 days at frozen storage temperature (−18±1°C). Nuggets coated with GTE incorporated coating solution had a lower TBA-reactive substances values, PVs, and TPCs when compared to the nuggets coated with SA and the control group.

Conclusion: Study revealed that incorporation of edible coatings with antioxidants, namely, GTE at 1% level had a significant effect in reducing the fat oxidation. The samples recorded a shelf life of 15 days under refrigerated storage when compared to their controls with 10 days of storage period and 75 days under frozen storage against controls with 60 days. T₁, T₂, and T₃ formulations had higher sensory scores in comparison to the controls. Overall acceptability scores of T₁ were higher when compared to the other formulations.

Keywords: chicken nuggets, edible coatings, green tea extract, sodium alginate.

Molecular characterization and combined genotype association study of bovine cluster of differentiation 14 gene with clinical mastitis in crossbred dairy cattle

Research (Published online: 01-07-2016)

1. Molecular characterization and combined genotype association study of bovine cluster of differentiation 14 gene with clinical mastitis in crossbred dairy cattle - A. Sakthivel Selvan, I. D. Gupta, A. Verma, M. V. Chaudhari and A. Magotra

Veterinary World, 9(7): 680-684

   doi: 10.14202/vetworld.2016.680-684

Abstract

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Aim: The present study was undertaken with the objectives to characterize and to analyze combined genotypes of cluster of differentiation 14 (CD14) gene to explore its association with clinical mastitis in Karan Fries (KF) cows maintained in the National Dairy Research Institute herd, Karnal.

Materials and Methods: Genomic DNA was extracted using blood of randomly selected 94 KF lactating cattle by phenolchloroform method. After checking its quality and quantity, polymerase chain reaction (PCR) was carried out using six sets of reported gene-specific primers to amplify complete KF CD14 gene. The forward and reverse sequences for each PCR fragments were assembled to form complete sequence for the respective region of KF CD14 gene. The multiple sequence alignments of the edited sequence with the corresponding reference with reported Bos taurus sequence (EU148610.1) were performed with ClustalW software to identify single nucleotide polymorphisms (SNPs). Basic Local Alignment Search Tool analysis was performed to compare the sequence identity of KF CD14 gene with other species. The restriction fragment length polymorphism (RFLP) analysis was carried out in all KF cows usingHelicobacter pylori 188I (Hpy188I) (contig 2) and Haemophilus influenzae I (HinfI) (contig 4) restriction enzyme (RE). Cows were assigned genotypes obtained by PCRRFLP analysis, and association study was done using Chi-square (χ2) test. The genotypes of both contigs (loci) number 2 and 4 were combined with respect to each animal to construct combined genotype patterns.

Results: Two types of sequences of KF were obtained: One with 2630 bp having one insertion at 616 nucleotide (nt) position and one deletion at 1117 nt position, and the another sequence was of 2629 bp having only one deletion at 615 nt position. ClustalW, multiple alignments of KF CD14 gene sequence with B. taurus cattle sequence (EU148610.1), revealed 24 nt changes (SNPs). Cows were also screened using PCR-RFLP with Hpy188I (contig 2) and HinfI (contig 4) RE, which revealed three genotypes each that differed significantly regarding mastitis incidence. The maximum possible combination of these two loci shown nine combined genotype patterns and it was observed only eight combined genotypes out of nine: AACC, AACD, AADD, ABCD, ABDD, BBCC, BBCD, and BBDD. The combined genotype ABCC was not observed in the studied population of KF cows. Out of 94 animals, AACD combined genotype animals (10.63%) were found to be not affected with mastitis, and ABDD combined genotyped animals was observed having the highest mastitis incidence of 15.96%.

Conclusion: AACD typed cows were found to be least susceptible to mastitis incidence as compared to other combined genotypes.

Keywords: cluster of differentiation 14, combined genotypes, Helicobacter pylori 188I, Haemophilus influenzae I, mastitis, single nucleotide polymorphisms.

Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat

Research (Published online: 30-06-2016)

22. Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat - Jai Prakash Khichar, Gyan Chand Gahlot, Vijay Kumar Agrawal, Kiran, Ajay Singh Dewna, Prakash and Mohammad Ashraf

Veterinary World, 9(6): 676-679

   doi: 10.14202/vetworld.2016.676-679

Abstract

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Aim: To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.

Materials and Methods: A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer’s protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.

Results: The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.

Conclusion: Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.

Keywords: exon 3, Marwari goat, myostatin gene, polymerase chain reaction-single strand conformation polymorphism.

Genetic analysis of first lactation production traits in Kankrej cattle

Research (Published online: 29-06-2016)

21. Genetic analysis of first lactation production traits in Kankrej cattle - K. J. Ankuya, N. K. Pareek, M. P. Patel, B. S. Rathod, K. B. Prajapati and J. B. Patel

Veterinary World, 9(6): 672-675

   doi: 10.14202/vetworld.2016.672-675

Abstract

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Aim: The aim was to estimate genetic factors affecting the first lactation milk production traits in Kankrej cattle of North Gujarat.

Materials and Methods: The 475 first lactation records of Kankrej cows that were maintained at the Livestock Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, over a period of 35 years from 1980 to 2014 were studied. The least squares maximum likelihood program was used to estimate genetic parameters of first lactation traits. Heritability was estimated through paternal half-sib analysis in adjusted data.

Results: The heritability estimate for production traits was 0.40±0.17, 0.45±0.17, 0.35±0.18, and 0.20±0.14 for standard 300 days milk yield (F300Y), total lactation milk yield (FLY), wet average (FWA), and lactation length (FLL), respectively, in the first parity. All the genetic and phenotypic correlations among different production efficiency traits were high and positive. Genetic correlations between F300Y and FLY, FLL, and FWA were 0.80±0.20, 0.59±0.16, and 0.81±0.32,where as the phenotypic correlations were 0.969, 0.688, and 0.868, respectively. Genetic correlations of FLY with FLL and FWA were 0.60±0.13 and 0.79±0.20, whereas the phenotypic correlations were 0.777 and 0.817, respectively. Genetic and phenotypic correlation between FLL and FWA was 0.63±0.28 and 0.31, respectively.

Conclusion: The heritability estimate of all first parity lactation traits waslow to medium (0.20-0.45) indicated the scope for further improvement in this trait through selection as well as managemental practice. Higher genetic and phenotypic correlation between thefirst lactation milk production traits gives theidea that genetic gain due to selection for one trait also givesmorecorrelated response of selection for other traits which is economically advantageous.

Keywords: correlation, heritability, first parity, Kankrej cattle.

The detrimental effects of lead on human and animal health

Review (Published online: 27-06-2016)

20. The detrimental effects of lead on human and animal health - Mohammed Abdulrazzaq Assi, Mohd Noor Mohd Hezmee, Abd Wahid Haron, Mohd Yusof Mohd Sabri and Mohd Ali Rajion

Veterinary World, 9(6): 660-671

   doi: 10.14202/vetworld.2016.660-671

Abstract

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Lead, a chemical element in the carbon group with symbol Pb (from Latin: Plumbum, meaning “the liquid silver”) and has an atomic number 82 in the periodic table. It was the first element that was characterized by its kind of toxicity. In animal systems, lead (Pb) has been incriminated in a wide spectrum of toxic effects and it is considered one of the persistent ubiquitous heavy metals. Being exposed to this metal could lead to the change of testicular functions in human beings as well as in the wildlife. The lead poising is a real threat to the public health, especially in the developing countries. Accordingly, great efforts on the part of the occupational and public health have been taken to curb the dangers of this metal. Hematopoietic, renal, reproductive, and central nervous system are among the parts of the human body and systems that are vulnerable toward the dangers following exposure to high level of Pb. In this review, we discussed the massive harmful impact that leads acetate toxicity has on the animals and the worrying fact that this harmful toxicant can be found quite easily in the environment and abundance. Highlighting its (Pb) effects on various organs in the biological systems, its economic, as well as scientific importance, with the view to educate the public/professionals who work in this area. In this study, we focus on the current studies and research related to lead toxicity in animals and also to a certain extent toward human as well.

Keywords: antioxidant, free radical, lead poisoning, oxidative stress.