Tuesday, 2 September 2014

Effect of dietary supplement of sugar beet, neem leaf, linseed and coriander on growth performance and carcass trait of Vanaraja chicken

2. Effect of dietary supplement of sugar beet, neem leaf, linseed and coriander on growth performance and carcass trait of Vanaraja chickenPunita Kumari, Chandramoni, Kaushalendra Kumar and Sanjay Kumar
Veterinary World, 7(9): 639-643


   doi: 10.14202/vetworld.2014.639-643


Punita Kumari: Department of Animal Nutrition, Bihar Veterinary College, Patna - 800 014, Bihar, India; puneeta.kri@gmail.com
Chandramoni: Department of Animal Nutrition, Bihar Veterinary College, Patna - 800 014, Bihar, India; chandramoni108@rediffmail.com
Kaushalendra Kumar: Department of Animal Nutrition, Bihar Veterinary College, Patna - 800 014, Bihar, India; drkaushalbvc@gmail.com
Sanjay Kumar: Department of Animal Nutrition, Bihar Veterinary College, Patna - 800 014, Bihar, India; sanjayvet29@rediffmail.com

Received: 11-03-2014, Revised: 04-07-2014, Accepted: 10-07-2014, Published online: 02-09-2014

Corresponding author: Punita Kumari, email: puneeta.kri@gmail.com



Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens

1. Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens - A. Balasubramaniam, M. Arthanari Eswaran, P. Suresh and K. Sukumar
Veterinary World, 7(9): 635-638


   doi: 10.14202/vetworld.2014.635-638


A. Balasubramaniam: Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India;
balasubramaniam72@gmail.com
M. Arthanari Eswaran: Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India;
drarthu@gmail.com
P. Suresh: Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India;vet_suresh@yahoo.com
K. Sukumar: Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India; drksugu@gmail.com

Received: 06-05-2014, Revised: 02-07-2014, Accepted: 12-07-2014, Published online: 02-09-2014

Corresponding author: A. Balasubramaniam, email: balasubramaniam72@gmail.com


Aim: The aim was to study the occurrence of tetracycline resistance determinant tetA gene, and antibacterial resistance pattern in commensal Escherichia coli recovered from healthy non-clinical layer chickens.
Materials and Methods: Twenty-four cloacal swabs were obtained from 15 flocks in five different layer farms located at around Namakkal, which is a place of high-intensity layer chicken rearing in south India. Identification of E. coli was carried out by performing cultural and biochemical tests. Antimicrobial resistance test was carried out using disc diffusion method. The polymerase chain reaction employingtetAC forward and tetAC reverse primers were carried out to detect tetA gene conferring resistance to tetracycline.
Result: All the collected cloacal swabs yielded E. coli. Twenty-one isolates (88%) were resistant to tetracycline antibiotic in disc diffusion method. All the isolates showed resistance to more than six antibiotics, which implied existence of multidrug resistant microbiota in intestine of poultry. Only seven (29%) isolates showed the presence of tetA gene indicating the involvement of either other one or more efflux gene(s), namely tetB, tetC and tetD or ribosomal protection encoded by tetM, tetO, tetQ and tetS genes than tetA gene.
Conclusion: Based on the presence of tetA gene among tetracycline-resistant bacteria in healthy non-clinical food-producing animals such as layer chickens, it can be significant in human medicine as tetA gene could easily be spread to other bacteria. This kind of phenomenon can be extrapolated in transfer of resistance for other antibacterial essential for treating bacterial infections in human. We conclude that preventing the spread of antimicrobial resistance through direct or indirect contact, consumed food/feed and through the environment is empirical in reduction of failures while treating bacterial infections.

Keywords: antibacterial resistance, Escherichia coli, tetA gene, tetracycline.

Sunday, 31 August 2014

Pandemic influenza preparedness and response - WHO guidance document

Pandemic influenza preparedness and response

WHO guidance document

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World Health Organization

Overview


WHO previously published pandemic preparedness guidance in 1999 and a revision of that guidance in 2005. Since 2005, there have been advances in many areas of preparedness and response planning. For example, stockpiles of antiviral drugs are now a reality and a WHO guideline has been developed to attempt to stop or delay pandemic influenza at its initial emergence. There is increased understanding of past pandemics, strengthened outbreak communications, greater insight on disease spread and approaches to control, and increasingly sophisticated statistical modeling of various aspects of influenza.
Extensive practical experience has been gained from responding to outbreaks of highly pathogenic avian influenza A (H5N1) virus infection in poultry and humans, and from conducting pandemic preparedness and response exercises in many countries. There is greater understanding that pandemic preparedness requires the involvement of not only the health sector, but the whole of society. In 2007, the International Health Regulations (2005) (IHR, 2005) entered into force providing the international community with a framework to address international public health concerns.
In light of these developments, WHO decided in 2007 to update its guidance to enable countries to be better prepared for the next pandemic. Therefore this document published in April 2009 replaces the WHO Global Influenza Preparedness Plan (WHO/CDS/CSR/GIP/2005.5). This document should be used in conjunction with the WHO checklist for influenza preparedness planning published by the World Health Organization in 2005.
This guidance has been superseded by the Pandemic Influenza Risk Management Guidance:

Evaluating the relationship between human and animal influenza viruses

Evaluating the relationship between human and animal influenza viruses

Assessing the relationship among multiple variants of influenza viruses circulating in animals and people and understanding their relative evolution in these hosts over time is key to improving vaccines for people and animals and for understanding the potential risk of future interspecies transmission events.



StripeyAnne on flickR, original name : The Pig Piper of Ham-lyn©
The latest OFFLU Swine Influenza Virus (SIV) Group meeting took place in Minneapolis, USA, 19-20 March 2013. The meeting had two major outcomes.
Characterization of the relationship between human and swine influenza viruses
Data on influenza viruses circulating in pig populations worldwide has been shared, but more effort is required for its compilation and analysis. According to the OFFLU SIV group, there is increasing scientific evidence from surveillance and genetic analyses that human seasonal influenza viruses and their variants have historically been introduced to pig populations by humans and are still circulating in different swine populations worldwide. The analyses of the HA protein of influenza viruses in pigs, a protein which plays a key role in virus-host interactions and in vaccine strain selection for human and animals, showed significant diversity among strains circulating within and between geographic areas. The maintenance of such diverse viruses within pigs for long periods of time has important implications for swine influenza vaccine strain selection and assurances of efficacy, which will have to be mainly tailored to the needs of the region. These viruses also pose a risk of re-introduction into the human population.
This global initiative requires further investment for compilation, analysis, and overall evaluating of the potential for human viruses to spread into swine and conversely for swine to be a reservoir for viruses that have human pandemic potential, and should be used in risk assessment for pandemic preparedness.
Proposed new HA gene cluster naming system for designating influenza viruses in pigs
The objective of this new nomenclature system developed and proposed by the OFFLU SIV group is to create a common language to be used by human and animal health sectors to designate swine influenza viruses on a global scale. This system will allow for viruses around the world to be evaluated by a unified set of criteria and their genetic relationships understood in a common context. Such a system is also important for targeting groups of viruses to study for antigenic properties and developing effective human and animal vaccines.
The proposed system is a phylogenetic classification system, distinguishing clusters of viruses that share common genetic characteristics. Therefore, it defines the relationship between the different influenza strains, which are continually evolving. This system enables the assessment of the genetic relationships between influenza viruses circulating in swine among different geographic regions as well as between human and swine seasonal influenza viruses.
The group will continue to work with the Influenza Research Database (fludb.org) to provide the HA gene cluster names based on the new system for future web-based cluster determination tools for swine HA sequences with free public access.
The OFFLU Network
OFFLU is a global open network of expertise on animal influenza established jointly in 2005 by the World Organisation for Animal Health (OIE) and the Food and Agriculture Organization of the United Nations (FAO) to support and coordinate global efforts to prevent, detect and control important influenzas in animals. OIE/FAO reference laboratories and world leading experts from a range of disciplines in animal and human health take part in the discussions. OFFLU collaborates with the World Health Organization (WHO) on issues relating to the animal-human interface, including pandemic preparedness for early preparation of human vaccine. 
The OFFLU Swine Influenza group started in 2010 and meets on an annual basis.
Source: 
http://www.oie.int/for-the-media/press-releases/detail/article/evaluating-the-relationship-between-human-and-animal-influenza-viruses/

Electrochemiluminescence immunoassay for progesterone by using a heterologous system in plasma bovine

11Electrochemiluminescence immunoassay for progesterone by using a heterologous system in plasma bovine - A. Ayad, M. Iguer Ouada and H. Benbarek
Veterinary World, 7(8): 610-613


   doi: 10.14202/vetworld.2014.610-613


A. Ayad: Department of Environment and Biological Sciences, Faculty of Life and Nature Sciences, University A. Mira, 06000, Bejaia, Algeria; Laboratory of Research on Local Animal Products, Veterinary Institute, Ibn Khaldoun University, 14000, Tiaret, Algeria; hanine06@gmail.com,abdelhanine.ayad@univ-bejaia.dz
M. Iguer Ouada: Department of Environment and Biological Sciences, Faculty of Life and Nature Sciences, University A. Mira, 06000,
Bejaia, Algeria; imokrane@gmail.com
H. Benbarek: Laboratory of Research on Local Animal Products, Veterinary Institute, Ibn Khaldoun University, 14000, Tiaret, Algeria; Department of Agricultural Sciences, Faculty of Life and Nature Sciences, University M. Istambouli, 29000, Mascara, Algeria; benbarekh@yahoo.com

Received: 12-05-2014, Revised: 27-07-2014, Accepted: 02-08-2014, Published online: 21-08-2014

Corresponding author: A. Ayad, email: hanine06@gmail.com, abdelhanine.ayad@univ-bejaia.dz


Aim: The present study describes the use of electrochemiluminescence (ECL) immunoassay method with specific kit human progesterone for measuring plasma progesterone in cattle.
Materials and Methods: Nine Holstein-Friesian females were selected and artificially inseminated (AI). Blood samples were collected from the coccygeal vessels into tubes containing EDTA at day 0 (n = 5, the control group) and day 90 (n = 4, diagnosed pregnant by rectal palpation) after AI. The day of AI was considered as day 0 for the calculation of pregnancy day. The samples were immediately centrifuged (15 min at 1500 × g), and the plasma was stored at 20°C until assay. The assay of progesterone was carried out by a method of competition immunological type in heterogeneous phase. The antibodies of capture and revelation are monoclonal specific to progesterone of human origin.
Results: The progesterone (P4) concentration in the whole female was physiological. The results of inter- and intraassay coefficients of variation were 16.6% and 6.7%, respectively. The values of accuracy and parallelism obtained were satisfactory.
Conclusion: The preliminary results show clearly that human progesterone ECL kit can be used to measure P4 in plasma bovine.

Keywords: assay, cow, electochemiluminescence, progesterone.

Saturday, 30 August 2014

Benefit cost analysis of Rhode Island Red chicken rearing in backyard on the basis of egg production performance

10. Benefit cost analysis of Rhode Island Red chicken rearing in backyard on the basis of egg production performance - P. K. Das, P. R. Ghosh, S. Pradhan, B. Roy and D. Mazumdar
Veterinary World, 7(8): 605-609


   doi: 10.14202/vetworld.2014.605-609


P. K. Das: Department of Veterinary Physiology, West Bengal University of Animal & Fishery Sciences, 37, K. B. Sarani, Kolkata - 700 037, West Bengal, India; pkdaskol@rediffmail.com
P. R. Ghosh: Department of Veterinary Physiology, West Bengal University of Animal & Fishery Sciences, 37, K. B. Sarani, Kolkata - 700 037, West Bengal, India; drprghosh@gmail.com
S. Pradhan: Department of Veterinary Pathology, West Bengal University of Animal & Fishery Sciences, 37, K. B. Sarani, Kolkata - 700 037, West Bengal, India; saktipadapradhan@gmail.com
B. Roy: Department of Animal Nutrition, West Bengal University of Animal & Fishery Sciences, 37, K. B. Sarani, Kolkata - 700 037, West Bengal, India; barunnutrition@yahoo.co.in
D. Mazumdar: Department of Agriculture Statistics, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India; debstat@gmail.com

Received: 29-04-2014, Revised: 01-07-2014, Accepted: 07-07-2014, Published online: 16-08-2014

Corresponding author: P. K. Das, email: pkdaskol@rediffmail.com



Thursday, 28 August 2014

Multistate Outbreak of Human Salmonella Infections Linked to Live Poultry in Backyard Flocks

Source: http://www.cdc.gov/salmonella/live-poultry-05-14/index.html


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