The relationship between ovarian hormones and mast cell distribution in the ovaries of dromedary camel (Camelus dromedaries) during the follicular wave

Research (Published online: 17-02-2023)

9. The relationship between ovarian hormones and mast cell distribution in the ovaries of dromedary camel (Camelus dromedaries) during the follicular wave

Ragab H. Mohamed, Nasra A. Yousef, Mahmoud Awad, Rasha S. Mohamed, Fatma Ali, Hassan A. Hussein, and Axel Wehrend

Veterinary World, 16(2): 309-316

ABSTRACT

Background and Aim: Mast cells (MCs) play an essential role in regulating tissue homeostasis through various non-allergic immune reactions. This study aimed to describe the salient features of MCs during different phases of the estrous cycle and evaluate the relationship between ovarian hormones and the presence of MCs in camel ovaries.

Materials and Methods: Genital tracts (n = 28) of healthy, non-pregnant camels were collected from a local slaughterhouse. The follicular wave stage was determined according to structures on the ovaries using an ultrasound device. Stages were classified as "growing" (n = 12, FØ = 0.3–0.8 cm), "mature" (n = 9, FØ = 0.9–2.2 cm), or "regression" phase (n = 7, FØ >2.5). Blood samples were collected at slaughter to determine serum estradiol-17β and progesterone levels using an immunoassay. Safranin-O, periodic acid/Schiff, alcian blue, or methylene blue stains were used to detect MCs.

Results: Follicular numbers at the growing, mature, and regression phases were determined to be 36, 14, and 7 follicles, respectively. Mast cells were widely but sparsely distributed within the ovarian tissue (9.3 MCs in the growing phase, 10.7 in the mature phase, and 7.0 in the regression phase). Typical histological features of MCs were observed in ovarian stromal tissue. Some MCs were found in the interstitial tissue, either near the follicular wall or the interstitial gland. Mast cells were present at a higher density during the mature phase than in the growing and regression phases in the ovarian matrix. A significantly reduced presence of MCs was found in the regression phase than in both the growing and mature phases (p < 0.05). A very strong positive correlation was observed between serum estradiol-17β concentrations and MC density in the ovaries (r = 0.9; p < 0.001). In addition, a strong negative correlation (r = –0.65; p = 0.03) was observed between the presence of MCs and serum progesterone concentrations.

Conclusion: These findings suggest that the follicular wave phase and the associated hormonal concentration induce changes in the number of MCs in the camel ovary.

Keywords: camel, follicular wave, immunohistochemistry, mast cell, ovarian hormones.