Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms

Research (Published online: 01-07-2021)

1. Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms

Eduard A. Shuralev, Nail I. Khammadov, Konstantin A. Osyanin, Inna A. Elizarova, Gaysha R. Salmanova, Nikolai D. Shamaev, Sergei V. Petrov, Clare Whelan, Nikolai Yu. Saushkin, Jeanne V. Samsonova, Ilsur G. Galimzyanov, Marina A. Efimova, Kamil S. Khaertynov, Tagir Kh. Faizov, Malik N. Mukminov and Arkadiy V. Ivanov

Veterinary World, 14(7): 1718-1726

ABSTRACT

Background and Aim: Several reports described the detection of specific caprine arthritis-encephalitis virus (CAEV) antibodies in Russian goat populations, which indicates the circulation of CAEV in Russian goat farms. The aim of this study was to use a multi-target approach to testing with both serological tests and an in-house real-time (RT) molecular test to investigate the prevalence of CAEV in goats from three hobbyist farms in the Republic of Tatarstan, Russia.

Materials and Methods: We applied a multi-target approach to testing with both enzyme-linked immunosorbent assay (ELISA) and an in-house RT polymerase chain reaction test to investigate the prevalence of CAEV in goats. Animals from the three hobbyist farms were used in this study. The animals from two farms (n=13 for F1 and n=8 for F2) had clinical signs of arthritis and mastitis. In the third farm (n=15 for F3), all goats were home-bred and had no contact with imported animals.

Results: CAEV antibodies (ELISA targets TM env and gag genes) were detected in serum samples from two farms (F1 and F2), indicating seroprevalence of 87.50-92.31%. Specific CAEV antibodies were also detected in milk samples. CAEV proviral DNA was detected in 53.85-62.50%. The results from all tests performed in the third farm (F3) were negative, indicating that all tests were 100% specific.

Conclusion: The results showed that CAEV is circulating and present in small hobbyist goat farms in Russia. Serological and molecular tests could be important for programs to control and eradicate CAEV in Russia for hobbyist goat farms.

Keywords: antibodies, antigens, caprine arthritis-encephalitis virus, goat, proviral DNA.