First report of PCR-based detection of Helicobacter species DNA in Camelus dromedarius in Egypt

Research (Published online: 17-09-2020)

21. First report of PCR-based detection of Helicobacter species DNA in Camelus dromedarius in Egypt

Ahmed Youssef, Ahmed Afifi, Ayman Hamed and Mohamed Enany

Veterinary World, 13(9): 1898-1901

ABSTRACT

Background and Aim: Helicobacter species infections have epidemiological and zoonotic impacts, and different species of Helicobacter have been implicated in infecting humans and animals. The aim of this study was to investigate Helicobacter species infections in Camelus dromedarius.

Materials and Methods: Fecal samples were collected from 32 camels from 9 camel farms located at Ismailia Governorate, Egypt. The collected samples were investigated by bacteriological isolation and conventional polymerase chain reaction (PCR) assays targeting the 16S ribosomal RNA gene.

Results: Although Helicobacter species could not be isolated from all the examined samples, Helicobacter DNA was detected in 2 (22.22%) of the 9 camel farms. Of the 32 camel fecal samples examined, 4 (12.5%) were positive for Helicobacter species as analyzed by the PCR assay.

Conclusion: To the best of our knowledge, this is the first report of PCR-based detection of Helicobacter species infections in C. dromedarius. Further epidemiological studies are required to clarify Helicobacter species infections in camels.

Keywords: Camelus dromedarius, Helicobacter, Non-pylori, polymerase chain reaction.