Isolation, culture, characterization, and osteogenic differentiation of canine endometrial mesenchymal stem cell

Research (Published online: 30-12-2017)

19. Isolation, culture, characterization, and osteogenic differentiation of canine endometrial mesenchymal stem cell

A. K. Sahoo, J. K. Das and S. Nayak

Veterinary World, 10(12): 1533-1541

ABSTRACT

Aim: In this study, the canine endometrium tissue is characterized for its stem cell properties such as adherence to tissue culture plate (plasticity), short population doubling time, serial clonal passaging, long-term culturing properties, stem cell marker expression, and multilineage differentiation potential.

Materials and Methods: The present work describes a novel isolation protocol for obtaining mesenchymal stem cells from the uterine endometrium and is compared with cells derived from umbilical cord matrix as a positive control. These cells are clonogenic, can undergo several population doublings in vitro, and can be differentiated to the osteocytes in mature mesenchymal tissues when grown in osteogenic differentiation media as detected by Alizarin Red-S staining.

Results: It is reported for the first time that the cells derived from the canine endometrium (e-multipotent stem cells [MSCs]) were able to differentiate into a heterologous cell type: Osteocytes, thus demonstrating the presence of MSCs. Thus, the endometrium may be told as a potential source of MSCs which can be used for various therapeutic purposes.

Conclusion: The endometrium can be used as a potential source of MSCs, which can be used for various therapeutic purposes.

Keywords: Alizarin Red-S staining, endometrium, mesenchymal stem cell, multipotent stem cells marker, osteogenic differentiation media.