Identification of non-cerebral cyst: Zoonotic Taenia multiceps in domestic goat in Bangladesh

Research (Published online: 01-10-2017)

1. Identification of non-cerebral cyst: Zoonotic Taenia multiceps in domestic goat in Bangladesh

Mohammad Omer Faruk, A. M. A. M. Zonaed Siddiki, Md. Shafiqul Islam, Azizunnesa Rekha, Sharmin Chowdhury, Md. Masuduzzaman and Mohammad Alamgir Hossain

Veterinary World, 10(10): 1156-1160

ABSTRACT

Aim: This study was performed to identify the non-cerebral Taenia multiceps cyst through molecular phylogeny of the 12S rRNA gene.

Materials and Methods: Eight cyst samples were collected from 385 examined slaughtered goats during October 2015-September 2016 from three slaughterhouses in Chittagong City Corporation. Cysts were removed from the thigh muscle, and scolices were collected for light microscopic examination and molecular identification. The DNA was extracted and analyzed by polymerase chain reaction using 12S rRNA gene primers. Cyst samples were also preserved in 10% buffered formalin for histopathological study.

Results: T. multiceps non-cerebral cyst is 2.1% prevalent in goat in this area. Under light microscopic examination, scolex was found with four suckers and a rostellum with the double crown of 32 hooks and hooklets. Molecularly, all the samples were amplified with 12S rRNA gene fragments yielded 270 base pair amplicon. Zenker's necrosis with focal to diffuse infiltration of lymphocytes and eosinophil was also found around the cyst wall in histopathological examination.

Conclusion: Although the non-cerebral form of the cysts produced by T. multiceps is genetically identical with the cerebral cyst, previously published data indicated that cerebral T. multiceps cyst is predominant in other parts of the world as well as in Bangladesh. This study showed that non-cerebral cyst is also prevalent in this country which is very important for public health concern. This study depicts an idea of non-cerebral form of zoonotic T. multiceps cyst which will be helpful in taenia cyst control and prevention.

Keywords: 12SrRNA, Bangladesh, goat, non-cerebral cyst, phylogeny, Taenia multiceps.