Research (Published online: 05-12-2016)
5. Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1. - S. S. Patil, A. Prajapati, D. Hemadri, K. P. Suresh, G. S. Desai, G. B. Manjunatha Reddy, B. M. Chandranaik, S. Ranganatha and H. Rahman
Veterinary World, 9(12): 1364-1369
doi: 10.14202/vetworld.2016.1364-1369
Aim: This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease.
Materials and Methods: Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software.
Results: A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain.
Conclusion: BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain.
Keywords: bovine herpesvirus 1-1, glycoprotein B, India, infectious bovine rhinotracheitis, phylogenetic analysis, sequencing, subtype 1.1.
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