Prevalence of Campylobacter jejuni and Campylobacter coli in raw milk and some dairy products

Research (Published online: 26-10-2016)

18. Prevalence of Campylobacter jejuni and Campylobacter coli in raw milk and some dairy products - Mona A. El-Zamkan and Karima G. Abdel Hameed

Veterinary World, 9(10): 1147-1151

   doi: 10.14202/vetworld.2016.1147-1151

Abstract

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Aim: This study was accomplished to test raw milk and certain dairy products sold in local markets of Qena, Egypt, for the presence ofCampylobacter coli and Campylobacter jejuni.

Materials and Methods: A total of 150 samples of raw milk, kareish cheese, and yoghurt (50 samples each) were subjected first to enrichment in Bolton broth at 42°C for 2 days under a microaerobic condition, subsequently campylobacter blood free selective agar plates were cultured and incubated in the same condition of the broth. Based on the morphological and biochemical themes of the growing colonies, it was further classified into Campylobacter spp. The identified isolates were later affirmed by polymerase chain reaction using primers that were designed to locate hipO genes in C. jejuni and glyA in C. coli.

Results: Of the total 150 examined samples of raw milk and soft cheese samples; 37 (24.6%) samples were contaminated withCampylobacter spp. C. jejuni was dominating in this study in 20%, 14%, and 8% of the examined raw milk, kareish cheese, and yoghurt samples, respectively. No sample harbored C. coli.

Conclusion: Campylobacter spp. could be detected in 24.6% of the investigated samples. C. jejuni isolated from 14% of the total tested samples, while C. coli could not be detected from the examined samples. Campylobacter spp. is rampant in the areas of poor hygienic conditions making products made from raw milk of public health hazard.

Keywords: Campylobacter coli, Campylobacter jejuni, dairy products, multiplex polymerase chain reaction, raw milk.