Differential response of immune-related genes to peptidoglycan and lipoteichoic acid challenge in vitro

Research (Published online: 17-09-2016)

11. Differential response of immune-related genes to peptidoglycan and lipoteichoic acid challenge in vitro - Sourabh Sulabh, Bharat Bhushan, Manjit Panigrahi, Ankita Verma, Naseer Ahmad Baba and Pushpendra Kumar

Veterinary World, 9(9): 983-988

   doi: 10.14202/vetworld.2016.983-988

Abstract

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Aim: To study the effect of Staphylococcus aureus cell wall antigens, peptidoglycan (PGN) and lipoteichoic acid (LTA) challenge on immune cells present in bovine peripheral blood mononuclear cells (PBMCs).

Materials and Methods: In this study, efforts have been made to investigate the effects of three combinations (10+10, 20+20 and 30+30 μg/ml) of PGN and LTA obtained from S. aureus. These antigens were used to challenge the bovine PBMCs. After 6 h of incubation quantitative, real time-polymerase chain reaction was used to study toll-like receptor 2 (TLR-2) and major cytokine mRNA expression in bovine PBMC challenged with three different antigen blends.

Results: The results indicated that mRNA level of interferon gamma is influenced by the expression of TLR-2 gene. Tumor necrosis factor-alpha (TNF-a), interleukin 10 (IL-10), and IL-8 genes showed a maximum response at a dose of 10 μg of PGN and 10 μg of LTA challenge per ml of culture medium. The outcome also suggests that both IL-10 and IL-8 followed the expression pattern of TNF-a.

Conclusion: A dose of 10 μg of PGN and 10 μg of LTA per ml of culture medium was found to be most suitable for challenging PBMC.

Keywords: expression, lipoteichoic acid, mastitis, peptidoglycan, peripheral blood mononuclear cell.