Research (Published online: 09-02-2015)
3. Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro - Gopal Puri, S. S. Chaudhary, V. K. Singh and A. K. Sharma
Veterinary World, 8(2): 143-146
doi: 10.14202/vetworld.2015.143-146
Aim: The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo.
Materials and Methods: Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitro culture in CO2 incubator.
Results: The average percentage of in vitro matured oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The results revealed a significant (p<0.05) increase in maturation rate of oocytes in FBS than EBS.
Conclusion: Buffalo oocytes were better in vitro matured in FBS than EBS.
Keywords: buffalo oocytes, estrus buffalo serum, fetal bovine serum, in vitro maturation.
No comments:
Post a Comment
Note: only a member of this blog may post a comment.