Research (Published online: 12-10-2014)

10. Association study of genetic variants at single nucleotide polymorphism rs109231409 of mannose-binding lectins 1 gene with mastitis susceptibility in Vrindavani crossbred cattle - V. N. Muhasin Asaf, Bharat Bhushan, Manjit Panigrahi, Prashant Dewangan, Amod Kumar, Pushpendra Kumar and G. K. Gaur

Veterinary World, 7(10): 807-810

   doi: 10.14202/vetworld.2014.807-810

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Abstract

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Aim: The present study was undertaken to identify whether single nucleotide polymorphism (SNP) rs109231409 located on mannose-binding lectins 1 (MBL1) gene was associated with mastitis tolerance/susceptibility.

Materials and Methods: After grouping 100 Vrindavani crossbred cattle as mastitis positive and negative animals, they were genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphisms method. Gene and genotype frequencies of different patterns were estimated by standard procedure (POPGENE version 1.32, (University of Alberta, Canada) and statistical analysis was carried out by logistic regression methods using STATA 12 software (StataCorp LP, USA).

Results: The 588 bp fragment of MBL1 gene was amplified using PCR. PCR product was digested with ApaI restriction enzyme showed two distinct genotypes viz., GG (311 bp and 272 bp fragments) and GA (588 bp, 311 bp and 277 bp fragments). The gene, genotype frequencies, average heterozygosity, polymorphic information content and χ2 values for the locus rs109231409 was ascertained.

Conclusions: No significant association between SNP “rs109231409” with mastitis tolerance was found. Although there is a lack of association, further studies have to be undertaken in a large population in order to validate the impact of rs109231409 (g.855G >A) on mastitis tolerance.

Keywords: mannose-binding lectins 1, mastitis, polymerase chain reaction-restriction fragment length polymorphisms, single nucleotide polymorphism.