Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury

Research (Published online: 30-06-2022)

25. Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury

Fabiola de Oliveira Paes-Leme, Eliana Matias de Souza, Mariah Gois Ceregatti, Marco Túlio Gomes Campos, Patricia Donado Vaz de Melo and Adriane Pimenta da Costa-Val

Veterinary World, 15(6): 1595-1600

ABSTRACT

Background and Aim: Acute kidney injury (AKI) is associated with a grave prognosis. A clinical assessment of kidney function can be performed based on the glomerular filtration rate (GFR). Cystatin C (CysC) can indicate the GFR or kidney function and its measurement is currently performed using immunological methods such as nephelometry, immunoturbidimetry, and enzyme-linked immunosorbent assays in human medicine. However, these techniques are not specific for use in veterinary medicine. This study aimed to validate an immunoturbidimetric assay for serum CysC (sCy) in dogs, determine the sCy reference intervals for healthy dogs, evaluate sCy stability in serum samples, and compare sCy with serum creatinine (sCr) in healthy dogs and dogs with AKI.

Materials and Methods: Forty-three dogs were divided into a control group (n = 19) and an AKI group (n = 24). An immunoturbidimetric method including commercially available human CysC calibrated with canine CysC was used to evaluate canine serum samples.

Results: An average recovery of 97% was observed for canine serum samples. The reference interval for CysC in healthy dogs was 0.57–1.29 mg/L. The sCy concentration in dogs with AKI was significantly higher (2.82 ± 1.46 mg/L) than in healthy dogs (0.93 ± 0.18 mg/L). Statistical analysis confirmed a strong correlation between sCy and sCr (r = 0.94; p < 0.05) in dogs with AKI.

Conclusion: The immunoturbidimetric method of evaluating sCy yielded satisfactory results and can be used for canine samples when a species-specific calibrator is used. Furthermore, sCy is a reliable marker of renal dysfunction in dogs. It is best to store samples for sCy evaluation at temperatures between 4°C and 8°C.

Keywords: canine, immunoturbidimetry, kidney.

Seroprevalence and risk factors of bluetongue virus in sheep of Chattogram, Bangladesh

Research (Published online: 30-06-2022)

24. Seroprevalence and risk factors of bluetongue virus in sheep of Chattogram, Bangladesh

Tahura Khanam Munmun, Shariful Islam, Shafayat Zamil, Md. Ashiqur Rahman, Josefina Abedin, Abdul Ahad and Ariful Islam

Veterinary World, 15(6): 1589-1594

ABSTRACT

Background and Aim: Bluetongue (BT) is a non-contagious, infectious disease of wild and domestic ruminant animals caused by the BT virus (BTV). Bangladesh having a border with a BTV-endemic country, India and a substantial number of susceptible animals. Therefore, this study aimed to estimate BTV seroprevalence and potential risk factors.

Materials and Methods: We collected 150 serum samples from indigenous sheep from Chattogram, Bangladesh. We screened the serum samples using a competitive enzyme-linked immunosorbent assay for detecting BTV-specific immunoglobulin.

Results: We detected antibodies against BTV in 39.3% (59/150; 95% confidence interval: 31.5–47.6) of all sampled sheep. Factors like sampling site, sheep rearing location, rearing sheep with other farm species, and body condition score had a significant (p < 0.05) influence on the seroprevalence of BTV.

Conclusion: The findings show that indigenous sheep have a higher BTV seroprevalence, necessitating sustained surveillance for early diagnosis and a better understanding of virus epidemiology in Bangladesh.

Keywords: Bangladesh, bluetongue virus, risk factors, seroprevalence, sheep.

Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line

Research (Published online: 29-06-2022)

23. Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line

Ida Bagus Ngurah Swacita, I. Wayan Suardana, I. Gusti Ngurah Sudisma and Hevi Wihadmadyatami

Veterinary World, 15(6): 1583-1588

ABSTRACT

Background and Aim: Breast cancer is the most common type of cancer in women because it attacks the productive age. Preliminary studies showed that lactic acid bacteria (LAB) strain SR6 from the Bali cattle colon has the potential to act as a superior probiotic. It is also assumed that its bacteriocin structure is specific and has a strong relationship with the specificity of the ligand and its biological activity at a receptor. Therefore, this study aims to assess the use of local LAB strains, which produce bacteriocins as anticancer agents, as well as to identify the bacteria as potent producers molecularly.

Materials and Methods: The study was initiated by cultivating LAB SR6 strain from stock isolates on De Man, Rogosa, and Sharpe (Oxoid, CM 0369, England) broth media. It was then confirmed molecularly through analysis of the 16S ribosomal ribonucleic acid gene. Subsequently, its anticancer activity was tested by assessing the cytotoxic activity in T47D cell culture using the 3-(4, 5 dimetiltiazol-2-yl)-2.5-diphenyl tetrazolium bromide (Invitrogen M6494, US) method.

Results: The results showed that the LAB strain SR6 was identified molecularly as Pediococcus pentosaceus. Furthermore, it had a toxic effect on T47D cells, which was indicated by the number of deaths after treatment with the extracellular protein of the strain, especially at the 50% total cell volume level.

Conclusion: Based on the toxic effect of the strain on human T47D cells, the LAB SR6 isolate, which was identified as P. pentosaceus has the potential to be developed as a good anticancer drug against breast cancer. However, there is a need to carry out an integrated study to fully explore the suitability of bacteriocins as in vivo therapeutics against the disease completely.

Keywords: apoptosis, breast cancer, lactic acid bacteria SR6 strain, necrosis.

Health comparison between guinea pigs raised in uncontrolled and controlled environments

Research (Published online: 29-06-2022)

22. Health comparison between guinea pigs raised in uncontrolled and controlled environments

Laksmindra Fitria, Nastiti Wijayanti, Tuty Arisuryanti and Siti Isrina Oktavia Salasia

Veterinary World, 15(6): 1575-1582

ABSTRACT

Background and Aim: Guinea pigs (GPs) (Cavia porcellus) are not only kept as pets but also widely used in biological and biomedical research. At present, GPs are also used as a species for animal-assisted therapy (AAT). Consequently, assessing their health status is vital to determining their quality of life, usability for research, and prevention of spread of potential zoonotic diseases to patients using them for AAT. GPs are mainly sourced from animal markets supplied by traditional farms, where environmental factors and sanitation are not properly controlled. This study aimed to compare health status between GPs raised in uncontrolled (conventional farm) and controlled (animal facility) environments.

Materials and Methods: Sample animals were obtained from a local animal market and transported to an animal facility. After 1 week of acclimatization, the health status of the animals, including general health condition, body weight, body temperature, complete blood count, liver function (alanine aminotransferase and bilirubin), renal function (blood urea nitrogen and creatinine), and presence of ectoparasites and endoparasites, was assessed. Then, the animals were maintained in the animal facility following the standard procedure for laboratory animals. After 2 months, the animals' health status was re-examined, assessing the same parameters.

Results: Based on the evaluated parameters, GPs raised in an uncontrolled environment were found to have poorer health status than those raised in a controlled environment. There were significant differences in almost all parameters between GPs raised in controlled and uncontrolled environments. We found that the populations of two ectoparasites, Gyropus ovalis and Gliricola porcelli, and one endoparasite, Eimeria caviae>, decreased significantly following the movement of the animals from an uncontrolled to a controlled environment.

Conclusion: GPs raised in an uncontrolled environment have poor health status. However, a controlled environment with better care management can improve the health status of GPs.

Keywords: clinical biochemistry, environment, guinea pig, health status, hematology, parasites.

Using bioelectrical impedance analysis for modeling regression and predicting body fat accumulation in dogs in East Thailand

Research (Published online: 28-06-2022)

21. Using bioelectrical impedance analysis for modeling regression and predicting body fat accumulation in dogs in East Thailand

Peera Arreesrisom, Thanmaporn Phichitrasilp, Nattakarn Naimon, Supochana Charoensin, Worawut Rerkamnuaychoke, Jumrueang Panpiansil and Thitichai Jarudecha

Veterinary World, 15(6): 1566-1574

ABSTRACT

Background and Aim: Obesity in dogs leads to several health problems, such as premature death, and contributes to other diseases. Recently, body fat percentage has been considered to represent the body condition of dogs, and bioelectrical impedance analysis (BIA) is the most effective method for accurately measuring body fat in dogs. In Thailand, information on the body condition of dogs is limited, and there is no standard body fat level for Thai or mongrel dogs. This study was designed to evaluate and analyze the body fat percentage in dogs through BIA using a handheld instrument. The results of this study can help enhance the quality of life and health of dogs and aid in setting a standard body fat level for Thai or mongrel dogs.

Materials and Methods: The body fat percentage of 340 Thai and mongrel dogs in East Thailand was measured in the standing position, and the body condition score (BCS) (range, 1–5), sex, sterilization status, age, type of diet, and lifestyle were recorded. A linear regression model was developed to compare the variables and the predicted body fat percentage, and multiple linear regressions were used to analyze the factors for body fat increment.

Results: The linear regression model used to estimate the percentage of body fat (y) for each BCS (x) was y = 0.84 + 8.36x (R2 = 0.7219; p < 0.0001); the average body fat percentage was 27.52% for all studied dogs; specifically, 24.83% for the Thai Bangkaew, 26.42% for the Thai Ridgeback, and 27.65% for mongrels. The median body fat percentage was significantly higher in female than in male dogs. We found that as age increases, body fat percentage also increases; this increasing trend begins at the age of 5 years. However, increasing the level of activity and decreasing meal frequency leads to an increase in body fat percentage in neutered male dogs.

Conclusion: The average body fat percentage of dogs in East Thailand is 27.52% and this value is expected to increase when these dogs reach the age of 5 years. BIA is a valid and effective measurement tool for detecting the body fat percentage in dogs.

Keywords: bioelectric impedance analysis, body fat, dog, Thailand.

First report of multidrug-resistant Salmonella Infantis in broiler litter in Tolima, Colombia

Research (Published online: 28-06-2022)

20. First report of multidrug-resistant Salmonella Infantis in broiler litter in Tolima, Colombia

Mayra A. Bonilla-Caballero, María P. Lozano-Puentes, María A. Ospina and Maryeimy Varón-López

Veterinary World, 15(6): 1557-1565

ABSTRACT

Background and Aim: Salmonella has been identified as one of the most widely distributed zoonotic pathogens in broiler litter. Multidrug-resistant strains have been isolated from salmonellosis outbreaks, compromising the success of their treatment. This study aimed to isolate and identify Salmonella spp. serovars in healthy broiler litter in Tolima (Colombia), determine their resistance to different antimicrobials, and detect genes associated with β-lactam resistance that could be useful to control Salmonella spp. in poultry.

Materials and Methods: In total, 45 broiler litter samples were collected. Salmonella spp. was isolated and identified using selective and differential culture media and biochemical tests. Molecular confirmation of the pathogen was performed with the invA gene and serotyping by Kauffman–White scheme. Antimicrobial susceptibility to 15 antibiotics was determined by Kirby–Bauer method. In cefotaxime-resistant strains, blaCTX-M-F, blaCTX-M-1, blaCMY, and blaTEM genes were evaluated by polymerase chain reaction (PCR).

Results: In total, 817 presumptive strains were obtained from xylose lysine deoxycholate and Salmonella Shigella agars and subcultured on xylose-lysine-tergitol 4 and MacConkey agars, from which 150 strains were isolated; 29 of these strains were presumptive for Salmonella spp. after performing biochemical tests and 16 were confirmed by PCR as Salmonella Infantis (15) and Gallinarum (1). All strains were found to be multiresistant to antibiotics, showing three different profiles and isolates resistant to cefotaxime, and the blaCTX-M gene was detected.

Conclusion: This is the first study to isolate S. Infantis from broiler litter in Colombia. All isolates exhibited resistance to the evaluated antimicrobials, suggesting the misuse of antimicrobials in small- and medium-sized poultry farms. The presence of Salmonella enterica serovar Infantis is a public health problem. Thus, regular monitoring of poultry litter is recommended, as these bacteria can be transmitted to humans through animal products or contaminated environments.

Keywords: antibiotics, cefotaxime, poultry, Salmonella.

Seroprevalence and risk factors associated with bovine herpesvirus 1 in dairy herds of Colombia

Research (Published online: 27-06-2022)

19. Seroprevalence and risk factors associated with bovine herpesvirus 1 in dairy herds of Colombia

Aura Daniela Ortiz-González, H. Alexander Lopez Buitrago, Diana María Bulla-Castañeda, D. Johana Lancheros-Buitrago, Diego Jose Garcia-Corredor, Adriana Maria Díaz-Anaya, Julio Cesar Tobón-Torreglosa, Diego Ortiz-Ortega and Martín Orlando Pulido-Medellín

Veterinary World, 15(6): 1550-1556

ABSTRACT

Background and Aim: Infectious bovine rhinotracheitis (IBR) is an infectious disease widely distributed globally and is considered the main cause of various reproductive and respiratory tract diseases in cattle and buffaloes. This study aimed to estimate seroprevalence and determine risk factors associated with the presentation of IBR in the municipality of Sotaquirá, Boyacá (Colombia).

Materials and Methods: A descriptive cross-sectional study with simple random sampling was performed, and the sample size was 1,000 cattle. Blood samples were obtained by coccygeal venipuncture and processed through indirect enzyme-linked immunosorbent assay using the Synbiotics® kit (Zoetis, New Jersey, USA) with a sensitivity and specificity of 96% and 98%, respectively. Data were processed using the statistical program EpiInfo® (Centers for Disease Control and Prevention; Atlanta, Georgia).

Results: A high seroprevalence of 57.5% was established. Seroprevalence was the highest in cattle >4 years of age (65.0% apparent seroprevalence [AS]; 67% true seroprevalence [TS]) and in the Holstein breed (65.5% AS; 67.8% TS). The breed and age of the animals were significantly associated with each other. The Holstein breed, age group >4 years, uncertified semen, and fetal death were established as risk factors for IBR. In comparison, the age groups of <1 and 1–2 years and the Normande breed were established as protective factors against the bovine herpesvirus-1 virus.

Conclusion: Management factors, such as livestock from other owners and animal purchases, which affect disease presentation, are evident. The implementation and development of novel prevention and control measures for IBR at the national level are necessary.

Keywords: cattle, cattle diseases, enzyme-linked immunosorbent assay, Infectious bovine rhinotracheitis.