Evaluation of sodium lauryl sulfate for the development of cow-side mastitis screening test

Research (Published online: 31-08-2021)

40. Evaluation of sodium lauryl sulfate for the development of cow-side mastitis screening test

Nobonita Sarker Tanni, Md. Shafiul Islam, Mojahidul Kabir, Mst. Sonia Parvin, Md. Amimul Ehsan and Md. Taohidul Islam

Veterinary World, 14(8): 2290-2295

ABSTRACT

Background and Aim: Subclinical mastitis (SCM) is an economically important disease for dairy cattle worldwide; therefore, regular screening is imperative to detect SCM at an early stage so as to control it. The study was conducted to compare the test characteristics of sodium lauryl sulfate (SLS) as a test reagent to detect SCM in dairy cows.

Materials and Methods: First, 106 milk samples of dairy cows were subjected to available indirect screening tests (white side test [WST], surf field mastitis test, Leucocytest, and Immucell) considering somatic cell count (SCC) as gold standard test. Then 273 milk samples were allowed to react with different concentrations of SLS with or without sodium hydroxide (NaOH) and indicators (bromothymol blue [BTB] and bromocresol purple [BCP]).

Results: SLS with or without NaOH yielded best reaction with the milk samples similar to Leucocytest. It was observed that the reaction of milk samples with SLS added with indicators (BTB and BCP) was easier to visualize than without indicators. SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP had high sensitivity, specificity, and substantial agreement with SCC. The area under the receiver operating characteristics curve of SLS 2% with BCP and SLS 3%+NaOH 1.5% with BTB was 0.917 and 0.875, respectively.

Conclusion: It may be concluded that SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP may be the potential reagents for the development of an effective cow-side test to detect SCM, as the main ingredient SLS is considerably cheap and readily available in developing countries.

Keywords: bovine subclinical mastitis, dairy cows, screening test, sensitivity, sodium lauryl sulfate, specificity.

Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi

Research (Published online: 31-08-2021)

39. Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi

Gina Saptiani, Slamet Budi Prayitno and Sari Anggarawati

Veterinary World, 14(8): 2282-2289

ABSTRACT

Background and Aim: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black tiger shrimp (Penaeus monodon).

Materials and Methods: A total of 330 shrimps were equally assigned into three extract forms (crude extract, ethyl acetate extract, and n-butanol extract, respectively) in which three levels were provided for each extract. Negative control (without leaf extract) and positive control (with oxytetracycline at 0.05 mg/mL) were used, giving a total of 11 experimental treatments.

Results: The results showed that shrimps induced into all form of leaf extracts had significantly higher survival rates, clinical symptoms, and pathological anatomy than those negative control (C–) and positive control (C+). Total hemocyte cells, granulocytes, percentage of phagocytic, and prophenoloxidase activity were similar among leaf extract treatments (p>0.05), but those groups were significantly higher than those of C– and C+ (p<0.05).

Conclusion: n-butanol leaf extract at 300 mg/L is suggested to be the most effective treatment since it showed the highest efficacy on the parameters observed. Thus, it is possible to use the leaf extract of A. ilicifolius on-farm as a strategy to enhance bacterial disease resistance and prevent mortality.

Keywords: Acanthus ilicifolius, herbal plant extract, immunity, Penaeus monodon.

Feeding value of sorghum stover fed to tropical hair sheep as complete rations in chop, mash, pellet, and block forms

Research (Published online: 30-08-2021)

38. Feeding value of sorghum stover fed to tropical hair sheep as complete rations in chop, mash, pellet, and block forms

J. Raju, J. Narasimha, N. Nalini Kumari, T. Raghunanadan, V. Chinni Preetam, A. Ashok Kumar and P. Ravi Kanth Reddy

Veterinary World, 14(8): 2273-2281

ABSTRACT

Background and Aim: Poor palatability, low bulk density, and low nutritive value restrict the utilization of the crop residues as animal feeds. Altering the physical characteristics of feed by blending the roughage and concentrates in the form of complete feed improves the nutrient use efficiency and reduces the feed wastage, feed cost, and labor cost. The study aims to determine suitable processing methods (mash, pellet, or block forms) for efficient utilization of sorghum stover-based complete rations vis-a-vis conventional feeding methods in sheep.

Materials and Methods: The sorghum stover was incorporated in complete rations with roughage to concentrate ratio of 50:50 proportion in a growth trial of 120 days. The feed ingredients were chaffed, ground in a hammer mill, passed through expander-extruder, and compressed by feed block machine to prepare chop, mash, pellet, and block form of rations, respectively. Twenty-four male intact growing Nellore×Deccani cross lambs (3.5±0.5 months age, 14.50±0.41 kg (mean ± SD) at the start of the experiment) were divided into four experimental groups of six animals each in a complete randomized design. The experimental rations were randomly allotted to each group and evaluated for their intake, nutrient utilization, and growth performance.

Results: The sheep fed on pellet-based ration consumed a higher (p<0.05) quantity of dry matter. The digestibility coefficients of organic matter, crude protein, and neutral detergent fiber were higher (p<0.05) in processed rations (mash, pellet, or block). Further, the nitrogen balance (g/d) was higher (p<0.05) in the mash, pellet, and block form of rations, compared to chopped ration. The processing method did not influence calcium or phosphorous balance parameters, except for their urinary losses, which showed an increasing trend (p=0.07). The ram lambs fed with pelleted ration showed higher (p<0.05) weight gain than mash, block, or chaff forms. The efficiency of feed utilization in gaining one kg body weight was higher (p<0.05) in lambs fed a pelleted diet. Feeding pelletized ration was more economical to gain one kg body weight. The bulk density was highest for block-based ration followed by pellet, mash, and conventional rations, and the carrying capacity of truck was highest with the least transport cost in block-form of rations.

Conclusion: Physical processing (mash, pellet, and block) of sorghum stover-based complete rations increased the nutrient utilization and growth performance of sheep compared to conventional chopped form. Pelleting the mash with expander-extruder procedure was found to be more profitable. Nevertheless, the cost economics revealed blocks as more preferable forms for transporting the complete rations to larger distances.

Keywords: block, crop residue, digestibility, feed processing, growth, lambs, mash, pellet.

Influence of first colostrum pasteurization on serum immunoglobulin G, iron, and activity of gamma-glutamyltransferase in newborn dairy calves

Research (Published online: 29-08-2021)

37. Influence of first colostrum pasteurization on serum immunoglobulin G, iron, and activity of gamma-glutamyltransferase in newborn dairy calves

Sebastian Ganz, Klaus Failing, Abdulwahed Ahmed Hassan, Michael Bülte and Axel Wehrend

Veterinary World, 14(8): 2267-2272

ABSTRACT

Background and Aim: Colostrum pasteurization is an established procedure in dairy farms in developed countries. This practice can improve the health status of the offspring by reducing several pathogens. This study aimed to focus on the pasteurization of bovine first colostrum and its influence on certain important bioactive components.

Materials and Methods: This study was conducted in Holstein-Friesian bull calves, which were randomly divided into two groups and fed with 6 L of untreated (UT, n=10) or 6 L of heat-treated (HT, 63.5°C for 30 min, n=10) colostrum from their own dam within the first 12 h after birth. Blood samples were taken before, 24 h, and 48 h after first colostrum intake to determine the concentrations of immunoglobulin G (IgG) and iron and the activity of gamma-glutamyltransferase (GGT) in the serum.

Results: The level of IgG was not affected by pasteurization (p=0.19). However, a slower increase in GGT activity (p<0.05) and a lower serum iron concentration (p=0.04) were observed in the HT group.

Conclusion: It can be concluded that pasteurization influences the absorption of colostrum components and therefore, the passive transfer of immunity, although the level of IgG was not affected by pasteurization in this study.

Keywords: calf, first colostrum, heat treatment, immunoglobulin G, iron deficiency anemia.

The first report on internal transcribed spacer region-based characterization of microfilaria in Asian elephants (Elephas maximus) in Thailand

Research (Published online: 28-08-2021)

36. The first report on internal transcribed spacer region-based characterization of microfilaria in Asian elephants (Elephas maximus) in Thailand

Choenkwan Pabutta, Nuttapon Bangkaew, Pratthana Inthawong, Pannarai Mahadthai, Waleemas Jairak, Nantana Soda, Manakorn Sukmak and Supaphen Sripiboon

Veterinary World, 14(8): 2260-2266

ABSTRACT

Background and Aim: Filarial infections can significantly impact the health of both humans and animals. In elephants, filariasis has been associated with cutaneous dermatitis and skin nodules. However, molecular evidence for such infections is limited in Thailand. This study aimed to identify the morphological and molecular characteristics of microfilaria in captive Asian elephants in Thailand.

Materials and Methods: Whole blood collected from the ear vein of 129 captive Asian elephants was hematologically analyzed, and the blood parasites were evaluated using three standard techniques: The microcapillary test, thin blood smears, and polymerase chain reaction (PCR).

Results: Conventional PCR revealed that approximately 17% (22/129) of the sampled elephants were positive for microfilaria. Microscopy revealed that microfilariae are large, unsheathed, with extended nuclei, a short headspace, and a curved tail tapering at the end. Results of internal transcribed spacer region analysis show that the elephant microfilariae are closely related to Onchocerca spp. All of the elephants positive for microfilaria presented with neither skin lesion nor anemic signs. Microfilaria infection was not associated with age; however, microfilariae were more likely to be detected in male elephants due to differences in management systems.

Conclusion: This is the first study to provide both morphological and molecular evidence of microfilaria in Thai elephants. There is an urgent need to investigate the long-term and large-scale effects of microfilaria on the health of elephants.

Keywords: Asian elephant, genotype, internal transcribed spacer, microfilaria, Thailand.

Assessment of wound area reduction on chronic wounds in dogs with photobiomodulation therapy: A randomized controlled clinical trial

Research (Published online: 28-08-2021)

35. Assessment of wound area reduction on chronic wounds in dogs with photobiomodulation therapy: A randomized controlled clinical trial

Somphong Hoisang, Naruepon Kampa, Suvaluk Seesupa and Supranee Jitpean

Veterinary World, 14(8): 2251-2259

ABSTRACT

Background and Aim: Chronic wounds are a clinical problem and require intensive standard wound care. However, this is sometimes insufficient to promote healing. Photobiomodulation therapy (PBMT) can be used as an adjunctive therapy to improve wound healing. Various PBMT devices with different properties and parameter settings as well as different animal species can influence a variety of clinical outcomes. This study aims to assess the use of 830 nm PBMT or simultaneous superpulsed and multiple wavelengths (SPMW; 660, 875, and 905 nm) PBMT on chronic wounds in client-owned dogs.

Materials and Methods: This study included 21 client-owned dogs with chronic wounds allocated into three groups: (1) Control group (C) treated with irrigated saline and without PBMT (n=7); (2) L1 group treated with irrigated saline together with the radiation of 830 nm PBMT (n=7); and (3) L2 group treated with irrigated saline together with the radiation of simultaneous SPMW-PBMT (n=7). Wound healing was assessed on the basis of wound size reduction as a percentage of wound area every 2nd day for 15 days using image analysis software (ImageJ software®, National Institutes of Health, Rockville, Maryland, USA).

Results: A significant difference in the percentage of wound area reduction was noted between the C and PBMT groups (L1 and L2; p<0.05). The average percentages of wound area reduction at the end of the study (15 days) were 42.39±20.58, 56.98±24.82, and 61.81±27.18 in the C, L1, and L2 groups, respectively. A steady decrease in wound size was noted in both PBMT and non-PBMT groups, and coefficients were 7.77, 8.95, and 10.01 in the C, L1, and L2 groups, respectively. The percentage of wound area reduction was found to be significantly different between the PBMT and non-BPMT groups on day 7 (p<0.05).

Conclusion: Based on the results of the current study, using either 830 nm PBMT or simultaneous SPMW-PBMT can accelerate the chronic wound healing process in dogs with a significant reduction in wound area. Therefore, it can be used as an adjunctive therapy to improve wound healing in dogs with reduced treatment duration.

Keywords: canine, chronic wound, combined wavelength photobiomodulation therapy, low-intensity laser therapy, photobiomodulation therapy.

Immunomodulatory, behavioral, and nutritional response of tryptophan application on poultry

Review (Published online: 28-08-2021)

34. Immunomodulatory, behavioral, and nutritional response of tryptophan application on poultry

Nguyen Thuy Linh, Budi Guntoro and Nguyen Hoang Qui

Veterinary World, 14(8): 2244-2250

ABSTRACT

Tryptophan is an essential amino acid for all animals that was discovered through casein hydrolysis. The use of tryptophan as feed additives has been attracting the attention of many nutritionists because it cannot be synthesized enough in an animal's body. Tryptophan or precursor to the vitamin niacin in the diet is important, and its supplementation for poultry is determined to improve the amino acid balance and promote the poultry's growth performance through enhancing appetite, feed efficiency, and protein synthesis. Moreover, poultry in different growth phases, breeding, and conditions require various amounts of tryptophan. In addition, supplemented tryptophan also improves the immune response or the immunomodulatory activity of poultry to various diseases through the kynurenine pathway, especially diseases in the bursa. Furthermore, tryptophan also has a strong relationship with lysine (the ideal tryptophan/lysine ratio) in improving growth performance. However, tryptophan deficiency could affect the behavioral responses (e.g. pecking behavior and poultry stress) because tryptophan serves as a precursor for the neurotransmitter serotonin and the pineal hormone melatonin in the diet. This paper tried to summarize all information about applying tryptophan in the diets and illustrate the roles of tryptophan in the poultry industry.

Keywords: nutrition, poultry's diet, poultry's performance, tryptophan.