Molecular and morphometric changes in the small intestine during hot and cold exposure in thermally manipulated broiler chickens

Research (Published online: 15-06-2021)

15. Molecular and morphometric changes in the small intestine during hot and cold exposure in thermally manipulated broiler chickens

Khaleel Emad Khaleel, Mohammad Borhan Al-Zghoul and Khaled Musa Mohammad Saleh

Veterinary World, 14(6): 1511-1528

ABSTRACT

Background and Aim: Thermal stress (hot or cold) is one of many environmental stressors that severely affects the health of broiler chickens. One negative effect of thermal stress is the disruption of the intestinal barrier function in broiler chickens. This study aimed to evaluate the effect of thermal manipulation (TM) on the small intestine in terms of histomorphometry as well as junctional, heat-shock, and immune response gene expression during post-hatch exposure to thermal stress.

Materials and Methods: The experiment was conducted by dividing 928 fertile Ross eggs into three incubation groups: The control (C) group (incubated at 37.8°C and 56% relative humidity [RH] for the whole incubation period), the TM using low temperature TML group (incubated at 36°C and 56% RH for 18 h/day from embryonic days 7 to 16), and the TM using high temperature (TMH) group (incubated at 39°C and 65% RH for 18 h/day from embryonic days 7 to 16). On post-hatch day 21, 90 chicks were randomly selected from each incubation group and were equally subdivided into three subgroups for the post-hatch thermal stress experiment: The TN subgroup (room temperature maintained at 24°C), the heat stress (HS) subgroup (room temperature maintained at 35°C), and the cold stress (CS) subgroup (room temperature maintained at 16°C). After 1 day of thermal stress exposure (age 22 days), five birds from each subgroup were euthanized and ileum samples were collected to evaluate the transcription of the Claudin (CLDN1), CLDN-5, Occludin, Cadherin-1, heat shock factors (HSF1), HSF3, 70 kilodalton heat shock protein, 90 kilodalton heat shock protein, Interleukin 6 (IL6), IL8, toll-like receptors-2 (TLR2), and TLR4 genes by Real-Time Quantitative Reverse Transcription polymerase chain reaction analysis. Finally, after 4 and 7 days of thermal stress (age 25 and 28 days, respectively), nine chicks were euthanized, and their jejunum and ileum were collected for histomorphometric analysis.

Results: After exposure to 1 day of thermal stress, the C subgroups exposed to thermal stress (HS and CS) possessed significantly increased expression of junctional, heat-shock, and immune response genes compared to the C-TN subgroup, and similar results were observed for the TMH. In contrast, thermally stressed TMH subgroups had significantly lower expression of the studied genes compared to C subgroups exposed to thermal stress. Furthermore, no significant changes were detected between the TML subgroups exposed to thermal stress and TML-TN. Moreover, significant alterations in villus height (VH), villus surface area, crypt depth (CD), and VH to CD ratio were observed between the TML, TMH, and C subgroups exposed to CS.

Conclusion: It might be suggested that TM may have a protective impact on the small intestine histomorphometry and epithelial integrity of broilers during post-hatch exposure to thermal stress.

Keywords: broilers, heat shock proteins, immune response, junctional proteins, thermal manipulation, thermal stress.

Post-operative radiographic measures of pelvic limb alignment in dogs with medial patellar luxation after trochlear wedge recession versus trochlear block recession surgery

Research (Published online: 11-06-2021)

14. Post-operative radiographic measures of pelvic limb alignment in dogs with medial patellar luxation after trochlear wedge recession versus trochlear block recession surgery

Radka Stayova Garnoeva and Mihail Dimitrov Paskalev

Veterinary World, 14(6): 1504-1510

ABSTRACT

Background and Aim: Anatomical and mechanical femoral angles are quite different among dog breeds. Most published data are about large dog breeds, however, medial luxation of patella is more common in small breeds. Measures of pelvic limb alignment are important for outcome of patellar luxation surgery. Therefore, the aim of the present study was to compare the values of anatomical and mechanical femoral and tibial angles in dogs before, immediately after, and 1 month after surgery for correction of medial patellar luxation (MPL).

Materials and Methods: The study was conducted with 54 dogs (67 stifle joints) from four small breeds that underwent surgery by either trochlear block recession (36 stifle joints) or trochlear wedge recession (31 stifle joints) techniques.

Results: In both trochleoplasty techniques, statistically significant differences in the values of the anatomical medial proximal femoral angle (aMPFA), anatomical lateral distal femoral angle (aLDFA), and femoral varus angle (in MPL Grade II) and of aMPFA and Q-angle (in MPL Grade III) were found out.

Conclusion: After block recession surgery, more angles were positively influenced and this effect was more pronounced in patients with MPL Grade II.

Keywords: medial patellar luxation, radiography, small dog breeds, trochlear block recession, trochlear wedge recession.

Ketone bodies – causes and effects of their increased presence in cows' body fluids: A review

Review (Published online: 11-06-2021)

13. Ketone bodies – causes and effects of their increased presence in cows' body fluids: A review

Piotr Guliński

Veterinary World, 14(6): 1492-1503

ABSTRACT

Ketosis is the most common metabolic disease in high-performance dairy cows during the first 6-8 weeks of lactation. Its main symptoms include an excessive amount of so-called ketone bodies in a cow's body fluids. Ketone bodies consist of β-hydroxybutyric acid (βHBA), acetoacetic acid, and acetone. βHBA is the main component with its share of the total volume of ketone bodies in the blood of about 70%. Clinical symptoms of ketosis in cows include loss of appetite, preference for forage to concentrated feed, and acetone odor in their mouth and urine. Those symptoms are accompanied by a production drop, an increase of concurrent illness (mastitis, metritis, and displaced abomasum), and poor reproductive performance. One of the ketosis characteristic effects is an increase in the level of fat in milk (>5%), while protein levels decrease (<2.9%). In the case of subclinical ketosis (SCK), the fat–protein ratio in milk is increased to above 1.4:1. The current consensus for SCK is to consider a cutoff point of βHBA to be at least 1.2 mmol/L in blood plasma. Ketosis prevention is based on keeping perinatal cows in good condition, that is, with around 3.5 points in the five-point body condition scoring, carefully balancing food doses during the first 2 months of lactation with the correct energy–protein ratio. Glucose precursor products should be administered orally, in particular to at-risk herds. Ketosis occurs in 7-14% on average of the total number of cows in a herd. In general, data on the prevalence of SCK vary considerably, depending on their source. Moreover, the problem is mostly observed in poorly-fed animals with high milk production potential. The objectives of this review are to reveal the current situation of ketosis prevalence, the possibility of diagnosis, consequences in dairy cows and to provide some recommendations for ketosis treatment and prevention.

Keywords: dairy cattle, ketone bodies, ketosis, metabolic disorders.

The impact of lamb diets containing either barley or corn on growth performance and carcass quality

Research (Published online: 10-06-2021)

12. The impact of lamb diets containing either barley or corn on growth performance and carcass quality

M. Ata and Belal S. Obeidat

Veterinary World, 14(6): 1487-1491

ABSTRACT

Background and Aim: Grains, such as barley (BAR) and corn (CORN), are major energy sources for small ruminants. This study aimed to assess the impact of feeding either BAR or CORN-based diets on growth performance and carcass characteristics.

Materials and Methods: Awassi male lambs, average body weight of 20.4±0.49 kg, were chosen randomly. Lambs were acclimated for 10 days and assigned to one of two diets (1) BAR and (2) CORN. Feeding continued for 70 days with 60 days of data collection. Daily intake was recorded. Measurements of body weight were taken starting from day 1 then once per week throughout the study period. On day 40, six lambs were randomly chosen from each group and placed in metabolism cages to assess digestibility and N balance. Lambs were slaughtered on the last day of the study to evaluate carcass characteristics and meat quality.

Results: Neutral and acid detergent fiber and ether extract intake were greater (p≤0.05) for lambs fed the BAR diets. Nitrogen loss in feces tended to be greater (p=0.09) for the CORN diet. Eye muscle depth (mm) tended to be greater (p=0.07) for the BAR diet. Nutrient digestibility, daily weight gain, carcass characteristics, and meat quality were not different (p≥0.1) between diets.

Conclusion: The results herein demonstrate that feeding BAR grain improved nutrient intake efficiency and consistency and did not affect weight gain and carcass traits. BAR-based diets might be a useful alternative to CORN for feeding growing lambs.

Keywords: Awassi lambs, barley, carcass, corn, performance.

Effect of Majapahit (Crescentia cujete L.) fruit powder on the immune profile of Litopenaeus vannamei after infection with Vibrio spp.

Research (Published online: 10-06-2021)

11. Effect of Majapahit (Crescentia cujete L.) fruit powder on the immune profile of Litopenaeus vannamei after infection with Vibrio spp.

Sri Rahmaningsih, Riska Andriani and Hernik Pujiastutik

Veterinary World, 14(6): 1480-1486

ABSTRACT

Background and Aim: The use of bioactive compounds is a promising tool to improve shrimp health regarding vibriosis. This study aimed to determine the effect of the dietary Majapahit (Crescentia cujete L.) fruit powder on the cellular immune response of vannamei shrimp (Litopenaeus vannamei) infected with Vibrio harveyi, Vibrio alginolyticus, and Vibrio parahaemolyticus.

Materials and Methods: Twelve vannamei shrimp (aged 1 month) were randomly divided into four groups (n=3). Vannamei shrimp received experimental feed with different concentrations of Majapahit fruit powder for 20 days: Group A, 0%; Group B, 3.04%; Group C, 6.08%, and Group D, 9.12%. Subsequently, vannamei shrimp was infected with V. harveyi, V. alginolyticus, and V. parahaemolyticus. The total hemocytes, total differential hemocytes (hyaline, semi-granular, and granular cells), and phagocytic activity were assessed. Data were analyzed through analysis of variance (p<0.05) using SPSS ver. 24 for Windows.

Results: Majapahit fruit powder at a dose of 3.04% increased the number of hyaline cells of L. vannamei after infection with Vibrio spp. Supplementation of the feed formula with Majapahit fruit powder at a dose of 3.04% increased the number of semi-granular and granular cells compared with the control. Furthermore, Majapahit fruit powder at doses of 3.04% and 6.08% increased the hemocytes compared with a dose of 9.12%. The phagocytic activity of L. vannamei after infection with Vibrio spp. tends to increase after supplementation with 3.04% Majapahit fruit powder.

Conclusion: This study showed that the addition of C. cujete L. powder to the feed formula increased the cellular immune response. The most effective dose ranged from 3.04% to 6.08%.

Keywords: Crescentia cujete L., hemocytes, immune response, phagocytosis.

Allele and genotype frequencies of the SOD1 gene polymorphism associated with canine degenerative myelopathy in Belgian Malinois dogs in Greece

Research (Published online: 09-06-2021)

10. Allele and genotype frequencies of the SOD1 gene polymorphism associated with canine degenerative myelopathy in Belgian Malinois dogs in Greece

Antonia Mataragka, John Ikonomopoulos, Georgios S. Zervas, Christos D. Vamvakidis, Nikolaos Tzimotoudis, Ariadne Loukia Hager-Theodorides, Maria Gazouli and Antonios Kominakis

Veterinary World, 14(6): 1472-1479

ABSTRACT

Background and Aim: Canine degenerative myelopathy (CDM) is an adult-onset fatal disorder associated with a point mutation of the superoxide dismutase 1 (SOD1) gene (SOD1:c.118G>A). This study aimed to determine the allele and genotype frequencies of this mutation in a group of Belgian Malinois dogs in Greece.

Materials and Methods: Samples (n=72) of whole blood were collected from 72 purebred dogs of the Hellenic Armed Forces; these samples were processed for DNA isolation, polymerase chain reaction, and digestion with the restriction endonuclease AcuI. Sample testing was conducted in compliance with ISO17025 accreditation requirements.

Results: The observed relative genotype frequencies were 71% for the homozygous (GG), 25% for the heterozygous (AG), and 4% for the homozygous mutant (AA) alleles. These frequencies were close to those expected, indicating no significant departure from Hardy–Weinberg equilibrium (HWE, p=0.395). The frequency of heterozygous animals indicates that a high risk of developing CDM in forthcoming generations exists in the tested population because mating among carriers would result in 25% AA progeny. The medical record of the group of study animals indicated selection against leishmaniosis, as applied throughout generations by owners and breeders. The potential association of this selection with the HWE status of the study population was discussed.

Conclusion: The SOD1:c.118G>A mutation was common in the tested group of dogs; thus, they are suitable for a follow-up investigation on the development and progression of CDM. A case-control study on animals with evidence of sensitivity to infectious myelopathy could provide new insights into disease pathogenesis.

Keywords: degenerative myelopathy, dogs, genetic analysis, genetic polymorphism, restriction fragment length polymorphism-polymerase chain reaction, SOD1:c.118A.

Tick (Acari: Ixodidae) infestation of cuscuses from Maluku Province, Indonesia

Research (Published online: 08-06-2021)

9. Tick (Acari: Ixodidae) infestation of cuscuses from Maluku Province, Indonesia

Prasetyarti Utami, Bambang Heru Budianto and Ana Sahara

Veterinary World, 14(6): 1465-1471

ABSTRACT

Background and Aim: Cuscuses are one of the endemic Indonesian marsupials, which needs to be protected and revived in terms of the numbers and range of species. Ectoparasites of ticks (Ixodidae) are one potential obstacle to cuscus conservation. Tick infestation can cause blood loss in the host, even being a predisposing factor for infection with pathogenic organisms. This study aimed to determine the prevalence, infestation intensity, and species of ticks present on cuscuses in Maluku Province, Indonesia.

Materials and Methods: Ticks were collected from cuscuses origin of the four regions in Maluku Province, namely the island of Ambon, Seram Island, Romang Island, and Wetar Island . Cuscuses were captured at night, with ticks being collected from them from the head to the tip of the tail. The tick samples obtained from the cuscuses were preserved, identified, and counted. Cuscuses were released back into their habitat after collecting the ticks. The obtained ticks were observed using an Olympus BX51 microscope with an Olympus DP12 digital camera and prepared for examination under a scanning electron microscope (SEM). Infestation rate, infestation intensity, and morphology of the species were described.

Results: The cuscuses were found to be infested with Ixodes cordifer ticks. Cuscuses in Maluku Province had a low tick infestation rate. The range of infestation prevalence of island origin cuscuses in Maluku was between 14.28% and 16.67%. Simultaneously, I. cordifer infestation level was mildly infested based on the intensity of thick infestation ranged from 1 to 1.2 ticks per cuscus. From observation of the tick surface structure under SEM, sexual dimorphism and various specific characteristics of the ticks were identified.

Conclusion: The low infestation rate of I. cordifer ticks in cuscus was influenced by the up and down movement of the conscious activity in the tree, which allowed minimal contact with the ticks. The infestation prevalence rates on each island studied were similar. Such similarities of infestation are related to the similarity of cuscus species among Ambon, Lakor, Seram, and Romang islands, which are all included in the Phalangeridae family, and their similar habitats, behaviors, climatic conditions, and geographical areas.

Keywords: Cuscus, Ixodes, scanning electron microscope.