The effect of Channa striata extract and standard eradication regimen on asymmetric dimethylarginine in Helicobacter pylori gastritis rat model

Research (Published online: 17-08-2020)

17. The effect of Channa striata extract and standard eradication regimen on asymmetric dimethylarginine in Helicobacter pylori gastritis rat model

OK Yulizal, Aznan Lelo, Syafruddin Ilyas and Raden Lia Kusumawati

Veterinary World, 13(8): 1605-1612

ABSTRACT

Background and Aim: The presence of gastric mucosa or submucosa inflammation due to Helicobacter pylori leads to histological changes. Gastric injury, pro-inflammatory factors, and oxidative stress in H. pylori infection produce asymmetric dimethylarginine (ADMA), which are a competitive inhibitor of nitric oxide synthase. Investigations were carried out aimed at finding new drugs derived from natural products for the treatment of H. pylori. Channa striata is known to have in vitro anti-inflammatory and antimicrobial properties. This study aimed to investigate the effect of C. striata extract and a standard eradication regimen on ADMA levels and histological changes in the H. pylori gastritis rat model.

Materials and Methods: Thirty-five male rats were randomly and equally divided into five groups. Group-1 was the negative control group and Groups-2 to 5 were H. pylori-infected groups. Groups-3 to 5 were administered C. striata extract, a standard eradication regimen, and a combination of standard eradication regimen and C. striata extract, respectively. Histological examination and serum ADMA levels were analyzed. The difference between groups was analyzed using the Kruskal–Wallis and one-way analysis of variance tests. The significance was p<0.05.

Results: Serum ADMA levels and severity of gastritis were higher in infected groups compared to the negative control group (p<0.05). The severity of gastritis and mean ADMA levels in the group that received a single administration of the C. striata extract was higher than the others (p<0.05). Serum ADMA levels and severity of gastritis were significantly reduced in the group that received a combination of standard eradication regimen and C. striata extract (p<0.05).

Conclusion: Single administration of C. striata extract worsens the severity of gastritis and increased serum ADMA levels in the H. pylori gastritis rat model. The administration of a combination of standard eradication regimen and C. striata extract reduces serum ADMA levels and significantly improves the severity of H. pylori gastritis rat model.

Keywords: asymmetric dimethylarginine, Channa striata, eradication regimen, Helicobacter pylori gastritis, histological changes.

Trypanocidal activity of methanol extracts of the hemolymph of Sarcophaga argyrostoma larva against Trypanosoma evansi infected mice

Research (Published online: 15-08-2020)

16. Trypanocidal activity of methanol extracts of the hemolymph of Sarcophaga argyrostoma larva against Trypanosoma evansi infected mice

Doaa S. Farghaly and Al-Shaimaa M. Sadek

Veterinary World, 13(8): 1599-1604

ABSTRACT

Background and Aim: Many natural products worldwide are used for medicinal purposes. Various insect-isolated compounds were investigated in pursuit of new therapeutic agents. This study aimed to compare the effects of methanol extract of hemolymph of Sarcophaga argyrostoma larvae with diminazene aceturate on some hematological and biochemical indices of mice infected with Trypanosoma evansi.

Materials and Methods: Sixteen albino mice were randomly divided into four groups, of four mice, which received different treatments: In Group 1 (G1), mice were infected intraperitoneally with 1×104 T. evansi and received no treatment (positive control), in Group 2 (G2), infected mice were treated with 0.5 mL/kg of diminazene aceturate, in Group 3 (G3), infected mice were treated with 0.5 mL/kg methanol extract of the hemolymph of S. argyrostoma larvae, and in Group 4 (G4), uninfected mice received 0.5 ml of distilled water (negative control). In G3, treatment was started 3 days before injecting the parasite, while for the other groups, a single dose of treatment was applied when the parasite appeared in the blood.

Results: Mice from G3 showed low parasitemia of 29×104/mm3 4 days post-infection until the infection completely disappeared on the 5th day, which was earlier than for other groups. The results showed that the numbers of red blood corpuscles (red blood cells [RBCs]) and white blood cells (WBCs) per unit volume were significantly different (p<0.05) between the four groups. The highest RBC (9.09×103 cell/ mm3) and WBC (14.30×103 cell/ mm3) counts were recorded in G3, whereas the lowest values of 6.60 and 4.60×103cell/ mm3, respectively, were recorded for G2. In addition, there were significant differences (p<0.05) between the different groups for platelet counts per unit volume, with G3 having the most (943×103 cell/ mm3) and G2 having the least (357×103 cell/ mm3). There was a significant (p<0.05) difference in the indices of biochemical activities between the extract-treated infected groups and the standard drug-treated group.

Conclusion: This study suggests that the methanol extract of the hemolymph of S. argyrostoma larva exhibits trypanocidal activity, so it may be exploited as a suitable candidate for the development of trypanocidal drugs.

Keywords: diminazene aceturate, hematology, methanol extract, Sarcophaga argyrostoma larva, trypanocidal activity, Trypanosoma evansi.

The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

Research (Published online: 15-08-2020)

15. The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

Muslim Akmal, Gholib Gholib, Mustafa Kamal Nasution, Sri Wahyuni, Rinidar Rinidar, Dian Masyitha and M. Aman Yaman

Veterinary World, 13(8): 1594-1598

ABSTRACT

Background and Aim: Testis (T) and epididymis (E) are waste from the abattoir that is rarely used. In fact, both organs contain important chemicals needed for spermatogenesis (e.g., hormones, proteins, and other molecules). Therefore, administration of a combination of testis and epididymis (CTE) extracts may activate androgen receptors (AR) and protein kinase A (PKA) molecules that play a prominent role in spermatogenesis. We, therefore, aimed at investigating the influence of the CTE extracts on the concentration of AR and PKA in male chicken.

Materials and Methods: This study used a completely randomized design with four treatment groups (K0, K1, K2, and K3) and five replications per group. K0 is a control group that received 1 mL normal saline, whereas K1, K2, and K3 are the test groups that received 1, 2, and 3 mL of CET extracts, respectively. Twenty male chickens (strain: broiler Mb 89), 3 weeks of age, weighing 500-700 g were used. We administered the injections in a 13-day period and on the 14th day; we collected and processed blood samples as serum to measure the AR and PKA concentrations using commercial chicken AR and PKA enzyme-linked immunosorbent assay kits, respectively. We performed analyses by analysis of variance using SPSS 20.0.

Results: The AR concentrations in K1, K2, and K3 groups increased by 4.26%, 10.97%, and 28.04%, respectively, compared to the K0 (control group). However, this increase was not significantly different between the groups (p>0.05). Moreover, the PKA concentrations increased by 2.97%, 2.60%, and 4.08% in K1, K2, and K3 groups, respectively, compared to the control group. However, this increase was not significantly different between the groups as well (p>0.05).

Conclusion: The CTE extracts tended to increase the AR and PKA concentrations even though it is not significant. Therefore, it needs further study when using the CTE extracts for spermatogenesis in male chicken.

Keywords: androgen receptor, chicken, epididymis and testicular extracts, protein kinase A, spermatogenesis.

Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis

Research (Published online: 14-08-2020)

14. Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis

Zuhair Bani Ismail and Sameeh M. Abutarbush

Veterinary World, 13(8): 1588-1593

ABSTRACT

Background and Aim: Mastitis is a common and economically important disease in dairy cattle. It remains one of the most common reasons for the extensive use of antimicrobials in dairy farms leading to the emergence of antimicrobial-resistant pathogens. The aim of this study was to determine the patterns of antimicrobial resistance of Escherichia coli isolates from bovine mastitis and to identify prominent antimicrobial resistance and virulence genes among isolated strains.

Materials and Methods: Antimicrobial susceptibility testing against six antibiotic groups, including tetracyclines, aminoglycosides, beta-lactams, macrolides, sulfonamides, and fluoroquinolones was performed using the disk diffusion method. PCR was performed on resistant isolates to detect resistance and virulence genes using commercially available primers.

Results: Out of 216 milk samples cultured, 14 samples yielded E. coli isolates. All isolates (100%) were resistant to ampicillin, amoxicillin, procaine penicillin, streptomycin, oxytetracycline, and sulfamethoxazole-trimethoprim. Only one isolate (7%) was sensitive to gentamicin, and all isolates (100%) were sensitive to enrofloxacin and ciprofloxacin. All isolates carried at least one resistance gene against one or more of the major antibiotic groups. All isolates carried the ereA, tetG, tetE, and tetB genes, followed by tetA (93%), ampC (86%), strA (86%), sul1 (78%), tetD (71%), tetC (57%), aadA (57%), and strB (36%). The lowest percentage of isolates carried bla1 (17%) and bla2 (12%) genes, and none of the isolates carried the qnrA gene. Most of the isolates (93%) carried the Shiga toxin 1 virulence gene, followed by complement resistance protein (79%), intimin (64%), Shiga toxin 2 (36%), cytotoxic necrotizing factor (35%), aerotaxis receptor (21%), and type 1 fimbriae (15%).

Conclusion: Results of this study indicate that the high percentages of E. coli isolate from bovine mastitis are resistant to two or more of the major antibiotic groups, irrespective of the presence or absence of relevant resistance or virulence genes.

Keywords: antimicrobial resistance, dairy cows, environmental mastitis pathogens, Escherichia coli.

Description of normal head structures of the one-humped camel (Camelus dromedarius) by magnetic resonance imaging, computed tomography, and cross-sectional anatomy

Research (Published online: 14-08-2020)

13. Description of normal head structures of the one-humped camel (Camelus dromedarius) by magnetic resonance imaging, computed tomography, and cross-sectional anatomy

Hassan Emam, Mohamed Aref, Ahmed Abdelbaset-Ismail, Ahmed Abdelaal, Shaimaa Gouda and Mohamed Gomaa

Veterinary World, 13(8): 1581-1587

ABSTRACT

Aim: This study was designed for the 1st time to describe the normal head structures of one-humped camel (Camelus dromedarius) using both magnetic resonance imaging (MRI) and computed tomography (CT) as well as cross-sectional anatomy.

Materials and Methods: Five fresh cadaver heads were collected from clinically normal camels and then subjected to T1-weighted MR and CT imaging. Afterward, these examined heads were transversely sliced to obtain seven crossing levels.

Results: The obtained structures per each crossing level were matched with their relevant sorted images of T1-weighted MRI and CT, then identified and labeled accordingly.

Conclusion: The data shown herein expand our knowledge of the normal head structures of the camel and could be used as a reference for ultimate diagnosis of the surgical affections of head using MRI and/or CT.

Keywords: camel, computed tomography, cross-leveling, head region, magnetic resonance imaging.

Seroprevalence study of peste des petits ruminants in sheep and goats in the northern region of India

Research (Published online: 13-08-2020)

12. Seroprevalence study of peste des petits ruminants in sheep and goats in the northern region of India

Vinayagamurthy Balamurugan, Bibitha Varghese, Kirubakaran Vinod Kumar, Dhanavelu Muthuchelvan, R. Dheeraj, Gurrappanaidu Govindaraj, Kuralayanapalya Puttahonnappa Suresh, Divakar Hemadri and Parimal Roy

Veterinary World, 13(8): 1573-1580

ABSTRACT

Background and Aim: Peste des petits ruminants (PPR) is a contagious, World Organization for Animal Health notifiable, economically important, transboundary morbilliviral disease of sheep and goats. Studying seroprevalence of PPR from different geographical areas under varying agro-climatic conditions may help in formulating effective and appropriate disease control strategies under the ongoing national PPR control program. The present cross-sectional study describes the prevalence of PPR virus antibodies in sheep and goats in the various epidemiological units in different states (Haryana, Himachal Pradesh [HP], Jammu and Kashmir [J&K], Punjab, Uttarakhand [UK], and Uttar Pradesh [UP]) of the northern region of India.

Materials and Methods: A total of 5843 serum samples (sheep [n=2463] and goats [n=3380]) were collected by stratified random sampling method from 322 epidemiological units in the studied region during 2017-2018 and tested for PPR virus (PPRV) antibodies by competitive ELISA.

Results: The results revealed that an overall seroprevalence of 44.05% (2574/5843) with 57.32%, 55.22%, 65.69%, 37.09%, 32.73%, and 29.35% prevalence of PPRV antibodies in small ruminants in Haryana, Punjab, UP, HP, J&K, and UK states, respectively. Further, Chi-squared test revealed an association of PPRV antibodies in goats (χ2=252.28, p<0.01) and sheep (χ2=192.12, p<0.01) across different states in the region.

Conclusion: The seroprevalence in majority of the epidemiological units (n=130) in sheep and goats in the studied region had <30%. This necessitates comprehensive, rigorous, continuous vaccination and active surveillance programs for few more years to achieve the desired 70% seroprevalence level of PPRV antibodies in population and to make the northern region of India, as PPR free zone.

Keywords: cross-sectional study, India, northern region, peste des petits ruminants, seroprevalence, sheep and goats.

Influence of antitox and Vitamin E-selenium on meat quality and safety in rabbits after 1,1-experimental dimethylhydrazine toxicosis

Research (Published online: 12-08-2020)

11. Influence of antitox and Vitamin E-selenium on meat quality and safety in rabbits after 1,1-experimental dimethylhydrazine toxicosis

Balgabay S. Maikanov, Mikhail V. Zabolotnykh, Laura T. Auteleyeva and Symbat P. Seidenova

Veterinary World, 13(8): 1567-1572

ABSTRACT

Aim: This study investigated the effects of antitox and Vitamin E-selenium on meat quality and safety in rabbits after experimental 1,1-dimethylhydrazine (1,1-DMH) toxicosis.

Materials and Methods: Experimental groups of rabbits weighing 1.5-2.5 kg each were kept in a vivarium under same controlled conditions (temperature 16-21°C, humidity 60-80%, 12/12 h light/dark cycle, noise level <85 dB) with free access to standard food containing 22.0% protein, 4.5% fat, and 4% fiber. The effect of a detoxifying mixture of antitox and Vitamin E-selenium on safety indicators (residual amount of 1,1-DMH) and quality indicators pre- and post-detoxification of the rabbits from 1,1-DMH was determined.

Results: After detoxification, the residual 1,1-DMH level decreased in all organs and tissues to <0.1 mg/kg. The nutritional value of meat increased by improving organoleptic, physical, and chemical parameters and the amino acid composition of protein.

Conclusion: The antitox+Vitamin E-selenium detoxifying mixture significantly decreases the residual 1,1-DMH level in organs and tissues of animals and increases the nutritional value of rabbit meat in subacute poisoning. The detoxifying mixture can also be used on animals grazing in territories adjacent to Proton-M launch sites for preventive and therapeutic purposes.

Keywords: 1,1-dimethylhydrazine, amino acid composition, detoxification, detoxifying mixture, meat, rabbits.