Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocidaassociated with pneumonia of goats in Chhattisgarh

Research (Published online: 24-02-2019)

22. Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocidaassociated with pneumonia of goats in Chhattisgarh

Nidhi Rawat, Varsha Rani Gilhare, Krishna Kumar Kushwaha, Deeksha Dipak Hattimare, Foziya Farzeen Khan, Rajesh Kumar Shende and Dhananjay Kumar Jolhe

Veterinary World, 12(2): 331-336

ABSTRACT

Aim: The purpose of this study was to isolate and characterize the Mannheimia haemolytica and Pasteurella multocida from blood, nasal discharge, and lung tissue of pneumonic goats.

Materials and Methods: A total of 14 goats were investigated for pneumonic pasteurellosis. Of 14 goats, nasal swabs and blood samples were collected from 10 clinically diseased animals. Moreover, lung tissue and heart blood samples were collected during necropsy of four goats died with pneumonia. All the samples were processed for the isolation of M. haemolytica and P. multocida in the laboratory. Bacterial isolates were identified by cultural and biochemical characters and 16S rRNA sequence analysis. All the isolates were subjected to susceptibility testing using commonly used antimicrobials. M. haemolytica isolates were characterized by PHSSA gene detection. P. multocida isolates were characterized by KMT1 gene detection and capsule typing.

Results: On necropsy of dead goats, the pneumonia was characterized as acute fibrinous bronchopneumonia. Bacterial culture revealed the isolation of M. haemolytica (7) and P. multocida (5) of 10 clinical cases. Moreover, M. haemolytica and P. multocida were coisolated from two of the lung tissues. Furthermore, one of the other two lung tissues showed the isolation of M. haemolytica while the other showed recovery of P. multocida. Bacterial isolates were specifically identified by the 16S rRNA sequence analysis. The isolates showed reduced susceptibility to β-lactams, aminoglycosides, and fluoroquinolones . Moreover, the PHSSA and KMT1 genes were specifically detected among M. haemolytica, and P. multocida isolates, respectively. All P. multocida isolates belonged to serogroup A.

Conclusion: The present study reported an occurrence of pneumonic pasteurellosis caused by M. haemolytica and P. multocida in a goat flock.

Keywords: bronchopneumonia, goat, Mannheimia haemolytica, Pasteurella multocida, pneumonic pasteurellosis.

Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus

Research (Published online: 23-02-2019)

21. Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus

M. Mebarki, R. Kaidi, A. Azizi and M. Basbaci

Veterinary World, 12(2): 325-330

ABSTRACT

Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse breeders. Relatively, a few studies have been conducted to determine fetal sex in mare using transrectal Doppler ultrasound. This study aimed to compare two sexing techniques, two-dimensional (2D) mode and color Doppler ultrasonography.

Materials and Methods: The study was conducted on 39 mares under field conditions. Examinations were performed using the ultrasonic model device (Medison SonoAce Pico, South Korea), equipped with real-time 3-7 MHz convex multifrequency transducer. Fetal sex diagnosis was carried out in two periods of pregnancy, early period (57-80 days of gestation) and late period (80-150 days of gestation).

Results: No difference (p=0.4) was observed between the efficiency of the 2D mode and Doppler ultrasound in sex determination with the respective frequency of 74% and 85%. The best time to sex the early fetus was between 57 and 70 days of gestation and between 90 and 120 days for the older fetus.

Conclusion: The accuracy of sex determination can be increased using Doppler mode compared to 2D mode, especially in the early period between 57 and 70 days, in male more than female sex.

Keywords: Doppler mode, fetal sex, mare, two-dimensional mode, ultrasound.

Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Research (Published online: 23-02-2019)

20. Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Nadia G. M. Ali, Ibrahim M. Aboyadak and Heba S. El-Sayed

Veterinary World, 12(2): 316-324

ABSTRACT

Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen.

Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography.

Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, and heart blood. S. epidermidis isolates were sensitive to OTC, ciprofloxacin, enrofloxacin, spiramycin, erythromycin (E), and florfenicol. B. cereus isolates were sensitive to all mentioned antibiotics except E. Based on the MIC test, all B. cereus isolates were sensitive to OTC with MIC ranging between <0.125 and 4 μg/ml and 11 S. epidermidis isolates were sensitive with MIC ranging between <0.125 and 8 μg/ml, while four isolates were resistant. Different degrees of degenerative changes were present in the hepatopancreas, posterior kidney, eye, and skin tissues of diseased fish.

Conclusion: Single intraperitoneal injection of long-acting OTC at a dose of 100 mg/kg body weight was effective in termination of S. epidermidis and B. cereus infection in white sea bream (D. sargus) broodstock.

Keywords: Bacillus cereus, histopathology, high-performance liquid chromatography, oxytetracycline, sensitivity, Staphylococcus epidermidis.

Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source

Research (Published online: 23-02-2019)

19. Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source

Andy Noorsaman Sommeng, R. Muhammad Yusuf Arya, Mikael Januardi Ginting, Diah Kartika Pratami, Heri Hermansyah, Muhamad Sahlan and Anondho Wijanarko

Veterinary World, 12(2): 309-315

ABSTRACT

Aim: This study aimed to investigate the antiviral activity of Pterois volitans phospholipase A2 (PV-PLA2) from Indonesia to human immunodeficiency virus (HIV).

Materials and Methods: Fresh venomous fin parts of wild PV specimens were collected from Java Sea waters. Then, it washed using phosphate buffer pH 7.0 and immersed in phosphate buffer pH 7.0 0.01 m containing CaCl2 0.001 m for 24 h. The immersed fin then allowed for extraction process by sonicating for 2×8 min with 80% pulse and 20 kHz output with temperature controlling to avoid denaturation. The crude venom (CV) extracted from the fin is allowed for purification by 80% ethanol (ET) precipitation and ammonium sulfate fractionation method. The purified PV-PLA2 then analyzed using Lowry's method, Marinette's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide assay. After determining the purest and safest sample of six samples analyzed, the chosen sample then tested into simian retrovirus-2 (SRV2)-A549 culture (48×104 cells/mL at 1-4 ppm), and compared to the CV sample (1-4 ppm) and lamivudine (100 ppm). The culture then is analyzed using a quantitative real time-polymerase chain reaction to find out the copy number of SRV-2 virus in each culture.

Results: The protein's activity, concentration, and purity analysis revealed that the PV-PLA2 purified using ammonium sulfate fractionation has the highest activity (1.81 times higher than the CV at 80% fractionation) and has higher purity than the sample from ET fractionation. The testing of the sample purified using ammonium sulfate fractionation at 80% saturation level shown that it has a 97.78% inhibition level toward SRV2-A549 culture at 4 ppm. However, in comparison to lamivudine which has 99.55% inhibition level at 100 ppm, it needs much lower concentration to achieve the same result.

Conclusion: The significant inhibition of SRV2-A549 culture shown that the PV-PLA2 extracted from PV venom has the potential to become anti-HIV substances. It would be worthwhile to further evaluate the antiretroviral activity of PV-PLA2 in the in vivo studies.

Keywords: antiretrovirus, lamivudine, phospholipase A2, Pterois volitans, simian retrovirus serotype 2.

Reproductive performance of pigs raised by intensive management system in Abuja, Nigeria

Research (Published online: 22-02-2019)

18. Reproductive performance of pigs raised by intensive management system in Abuja, Nigeria

Kenneth Owoicho Abah, Joy Iyojo Itodo, Simon Azubuike Ubah and Ibrahim Shettima

Veterinary World, 12(2): 305-308

ABSTRACT

Background: Population growth led to an increase in the number of people raising pigs, resulting in increased demand for piglets/pigs for breeding and pork for consumption.

Aim: This study was carried out to determine the reproductive performance of pigs raised by the intensive management system in Abuja, Nigeria, with a view to assist farmers in ensuring improved productivity and profitability.

Materials and Methods: Using an interview-based questionnaire, data from 121 sows and 649 preweaning piglets were collected in 12 herds, from September 2017 to March 2018. Measures of reproductive and production performance assessed in this study were interfarrowing interval (IFI), number of liveborn piglets (NLB), preweaning piglet mortality (PPM), age at weaning (AAW), weaning to service interval (WSI), age at first farrowing (AFF), number of piglets weaned per litter (NPWL), and number of piglets weaned per sow per year (NPWPY).

Results: The results obtained in this study were IFI 6.2±0.84 months, NLB 7.2±1.11, PPM 31%, AAW 40.2±3.12 days, NPWL 5.3±0.73, WSI 39.4±4.59 days, AFF 9.1±0.60 months, and NPWPY 8.1±1.21. The identified causes of PPM were maternal overlay 31.34%, splay leg/hypoglycemia 22.39%, cannibalism 20.40%, starvation 14.93%, and unknown cause 10.94%.

Conclusion: The result showed that the reproductive performance of the sow (especially, NPWPY and PPM) needs to be improved on. There is a need to promote extension and herd health services by veterinarians and livestock personnel to potential and existing farmers in the area. This is more so because organized pig production in the studied area is relatively new and more people are establishing pig farms in the studied area.

Keywords: Abuja, intensive management system, reproductive performance, swine production.

Assessment of safety and quality of fermented milk of camels, cows, and goats sold and consumed in five localities of Burkina Faso

Research (Published online: 22-02-2019)

17. Assessment of safety and quality of fermented milk of camels, cows, and goats sold and consumed in five localities of Burkina Faso

Hama Cissé, Jean Ulrich Muandze-Nzambe, Namwin Siourime Somda, Adama Sawadogo, Soungalo Moustapha Drabo, Francois Tapsoba, Cheikna Zongo, Yves Traore and Aly Savadogo

Veterinary World, 12(2): 295-304

Background and Aim: Fermented milk is food produced and consumed all over the world and plays an important role in human nutrition. This work aimed to evaluate the microbiological and physicochemical quality and mineral composition of fermented milk consumed in Burkina Faso.

Materials and Methods: A total of 114 samples of fermented milk from camels, goats, and cows were purchased in the market in five localities in Burkina Faso; Bobo Dioulasso, Djibo, Dori, Gorom-Gorom, and Sebba. Microbiological and physical parameters were monitored using standards methods.

Results: Microbiological analysis of fermented milks showed high average values of 7.60±1.50×109 colony-forming unit per milliliter (CFU/ml), 5.72±3.60×107 CFU/ml, 5.53±2.00×105 CFU/ml, 1.97±0.18×103 CFU/ml, 1.98±0.25×103 CFU/ ml, and 0.10±0.09×103 CFU/ml for total microbial flora, lactic acid bacteria, yeasts and molds, Staphylococcus aureus, total coliforms, and thermotolerant coliforms, respectively. None of the samples were contaminated by Salmonella or Shigella. The average values of pH, acidity, dry matter, ash, fats, proteins, and total carbohydrates content of samples were ranged, respectively: 3.830-4.137, 1.888-2.822%, 8.271-13.004%, 0.199-0.476%, 1.210-3.863%, 2.125-3.764%, and 3.080- 5.428 % (w/w). Na/K and Ca/Mg ratio ranged from 0.104 to 0.909 and from 3.392 to 16.996, respectively. Total microbial flora, yeasts and molds, total coliforms, fats, calcium, potassium, iron, and zinc were significantly different.

Conclusion: This research contributed in the evaluation of the hygienic and nutritional qualities of local fermented milk. Results obtained in this study confirm the need to set up the training program on the sanitary condition to traditional maker's to ensure the good fermented milk with high organoleptic and nutritional qualities.

Keywords: Burkina Faso, camel, cow, fermented milk, goat, sanitary quality.

Toxocara vitulorum cuticle glycoproteins in the diagnosis of calves' toxocariasis

Research (Published online: 20-02-2019)

16. Toxocara vitulorum cuticle glycoproteins in the diagnosis of calves' toxocariasis

Eman E. El Shanawany, Soad E. Hassan, Adel A.- H. Abdel-Rahman and Eman H. Abdel-Rahman

Veterinary World, 12(2): 288-294

ABSTRACT

Aim: The current study was designed to isolate and characterize Toxocara vitulorum glycoprotein antigens and then to evaluate its potency in accurate diagnosis of toxocariasis.

Materials and Methods: T. vitulorum glycoprotein fractions were isolated using Con-A affinity chromatography. The fractions characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and immunoblot assay. Mass spectrometric analysis was used for identification of proposed structure of the N-acetylglucosamine (GlcNAc) fraction. Enzyme-linked immunosorbent assay (ELISA) was used to assess the diagnostic potential of the isolated fractions.

Results: Surface of T. vitulorum adult worm revealed two glycoprotein fractions rich in glucose (Glc) and GlcNAc. Three bands of molecular weight 212kDa, 107 kDa, and 93 kDa were detected in Glc fraction by SDS-PAGE. These bands were also detected in GlcNAc fraction with an additional band of 49 kDa. GlcNAc fraction showed more diagnostic potency of calves' toxocariasis; 79% than Glc fraction; 46.9% by indirect ELISA. The additional band of 49 kDa in GlcNAc fraction is probably responsible for its higher diagnostic potentials. Western blotting verified the immunoreactivity of the Glc and GlcNAc isolated fraction as they reacted with calves sera infected with toxocariasis. The proposed structure of GlcNAc fraction was Ser-Meth-Arg-O-methylated GlcNAc.

Conclusion: GlcNAc-rich fraction of T. vitulorum can be successfully utilized in the diagnosis of calves' toxocariasis.

Keywords: affinity chromatography, calves toxocariasis, Con A, enzyme-linked immunosorbent assay, mass spectrometric analysis, N-acetylglucosamine.