Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source

Research (Published online: 23-02-2019)

19. Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source

Andy Noorsaman Sommeng, R. Muhammad Yusuf Arya, Mikael Januardi Ginting, Diah Kartika Pratami, Heri Hermansyah, Muhamad Sahlan and Anondho Wijanarko

Veterinary World, 12(2): 309-315

ABSTRACT

Aim: This study aimed to investigate the antiviral activity of Pterois volitans phospholipase A2 (PV-PLA2) from Indonesia to human immunodeficiency virus (HIV).

Materials and Methods: Fresh venomous fin parts of wild PV specimens were collected from Java Sea waters. Then, it washed using phosphate buffer pH 7.0 and immersed in phosphate buffer pH 7.0 0.01 m containing CaCl2 0.001 m for 24 h. The immersed fin then allowed for extraction process by sonicating for 2×8 min with 80% pulse and 20 kHz output with temperature controlling to avoid denaturation. The crude venom (CV) extracted from the fin is allowed for purification by 80% ethanol (ET) precipitation and ammonium sulfate fractionation method. The purified PV-PLA2 then analyzed using Lowry's method, Marinette's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide assay. After determining the purest and safest sample of six samples analyzed, the chosen sample then tested into simian retrovirus-2 (SRV2)-A549 culture (48×104 cells/mL at 1-4 ppm), and compared to the CV sample (1-4 ppm) and lamivudine (100 ppm). The culture then is analyzed using a quantitative real time-polymerase chain reaction to find out the copy number of SRV-2 virus in each culture.

Results: The protein's activity, concentration, and purity analysis revealed that the PV-PLA2 purified using ammonium sulfate fractionation has the highest activity (1.81 times higher than the CV at 80% fractionation) and has higher purity than the sample from ET fractionation. The testing of the sample purified using ammonium sulfate fractionation at 80% saturation level shown that it has a 97.78% inhibition level toward SRV2-A549 culture at 4 ppm. However, in comparison to lamivudine which has 99.55% inhibition level at 100 ppm, it needs much lower concentration to achieve the same result.

Conclusion: The significant inhibition of SRV2-A549 culture shown that the PV-PLA2 extracted from PV venom has the potential to become anti-HIV substances. It would be worthwhile to further evaluate the antiretroviral activity of PV-PLA2 in the in vivo studies.

Keywords: antiretrovirus, lamivudine, phospholipase A2, Pterois volitans, simian retrovirus serotype 2.

Reproductive performance of pigs raised by intensive management system in Abuja, Nigeria

Research (Published online: 22-02-2019)

18. Reproductive performance of pigs raised by intensive management system in Abuja, Nigeria

Kenneth Owoicho Abah, Joy Iyojo Itodo, Simon Azubuike Ubah and Ibrahim Shettima

Veterinary World, 12(2): 305-308

ABSTRACT

Background: Population growth led to an increase in the number of people raising pigs, resulting in increased demand for piglets/pigs for breeding and pork for consumption.

Aim: This study was carried out to determine the reproductive performance of pigs raised by the intensive management system in Abuja, Nigeria, with a view to assist farmers in ensuring improved productivity and profitability.

Materials and Methods: Using an interview-based questionnaire, data from 121 sows and 649 preweaning piglets were collected in 12 herds, from September 2017 to March 2018. Measures of reproductive and production performance assessed in this study were interfarrowing interval (IFI), number of liveborn piglets (NLB), preweaning piglet mortality (PPM), age at weaning (AAW), weaning to service interval (WSI), age at first farrowing (AFF), number of piglets weaned per litter (NPWL), and number of piglets weaned per sow per year (NPWPY).

Results: The results obtained in this study were IFI 6.2±0.84 months, NLB 7.2±1.11, PPM 31%, AAW 40.2±3.12 days, NPWL 5.3±0.73, WSI 39.4±4.59 days, AFF 9.1±0.60 months, and NPWPY 8.1±1.21. The identified causes of PPM were maternal overlay 31.34%, splay leg/hypoglycemia 22.39%, cannibalism 20.40%, starvation 14.93%, and unknown cause 10.94%.

Conclusion: The result showed that the reproductive performance of the sow (especially, NPWPY and PPM) needs to be improved on. There is a need to promote extension and herd health services by veterinarians and livestock personnel to potential and existing farmers in the area. This is more so because organized pig production in the studied area is relatively new and more people are establishing pig farms in the studied area.

Keywords: Abuja, intensive management system, reproductive performance, swine production.

Assessment of safety and quality of fermented milk of camels, cows, and goats sold and consumed in five localities of Burkina Faso

Research (Published online: 22-02-2019)

17. Assessment of safety and quality of fermented milk of camels, cows, and goats sold and consumed in five localities of Burkina Faso

Hama Cissé, Jean Ulrich Muandze-Nzambe, Namwin Siourime Somda, Adama Sawadogo, Soungalo Moustapha Drabo, Francois Tapsoba, Cheikna Zongo, Yves Traore and Aly Savadogo

Veterinary World, 12(2): 295-304

Background and Aim: Fermented milk is food produced and consumed all over the world and plays an important role in human nutrition. This work aimed to evaluate the microbiological and physicochemical quality and mineral composition of fermented milk consumed in Burkina Faso.

Materials and Methods: A total of 114 samples of fermented milk from camels, goats, and cows were purchased in the market in five localities in Burkina Faso; Bobo Dioulasso, Djibo, Dori, Gorom-Gorom, and Sebba. Microbiological and physical parameters were monitored using standards methods.

Results: Microbiological analysis of fermented milks showed high average values of 7.60±1.50×109 colony-forming unit per milliliter (CFU/ml), 5.72±3.60×107 CFU/ml, 5.53±2.00×105 CFU/ml, 1.97±0.18×103 CFU/ml, 1.98±0.25×103 CFU/ ml, and 0.10±0.09×103 CFU/ml for total microbial flora, lactic acid bacteria, yeasts and molds, Staphylococcus aureus, total coliforms, and thermotolerant coliforms, respectively. None of the samples were contaminated by Salmonella or Shigella. The average values of pH, acidity, dry matter, ash, fats, proteins, and total carbohydrates content of samples were ranged, respectively: 3.830-4.137, 1.888-2.822%, 8.271-13.004%, 0.199-0.476%, 1.210-3.863%, 2.125-3.764%, and 3.080- 5.428 % (w/w). Na/K and Ca/Mg ratio ranged from 0.104 to 0.909 and from 3.392 to 16.996, respectively. Total microbial flora, yeasts and molds, total coliforms, fats, calcium, potassium, iron, and zinc were significantly different.

Conclusion: This research contributed in the evaluation of the hygienic and nutritional qualities of local fermented milk. Results obtained in this study confirm the need to set up the training program on the sanitary condition to traditional maker's to ensure the good fermented milk with high organoleptic and nutritional qualities.

Keywords: Burkina Faso, camel, cow, fermented milk, goat, sanitary quality.

Toxocara vitulorum cuticle glycoproteins in the diagnosis of calves' toxocariasis

Research (Published online: 20-02-2019)

16. Toxocara vitulorum cuticle glycoproteins in the diagnosis of calves' toxocariasis

Eman E. El Shanawany, Soad E. Hassan, Adel A.- H. Abdel-Rahman and Eman H. Abdel-Rahman

Veterinary World, 12(2): 288-294

ABSTRACT

Aim: The current study was designed to isolate and characterize Toxocara vitulorum glycoprotein antigens and then to evaluate its potency in accurate diagnosis of toxocariasis.

Materials and Methods: T. vitulorum glycoprotein fractions were isolated using Con-A affinity chromatography. The fractions characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and immunoblot assay. Mass spectrometric analysis was used for identification of proposed structure of the N-acetylglucosamine (GlcNAc) fraction. Enzyme-linked immunosorbent assay (ELISA) was used to assess the diagnostic potential of the isolated fractions.

Results: Surface of T. vitulorum adult worm revealed two glycoprotein fractions rich in glucose (Glc) and GlcNAc. Three bands of molecular weight 212kDa, 107 kDa, and 93 kDa were detected in Glc fraction by SDS-PAGE. These bands were also detected in GlcNAc fraction with an additional band of 49 kDa. GlcNAc fraction showed more diagnostic potency of calves' toxocariasis; 79% than Glc fraction; 46.9% by indirect ELISA. The additional band of 49 kDa in GlcNAc fraction is probably responsible for its higher diagnostic potentials. Western blotting verified the immunoreactivity of the Glc and GlcNAc isolated fraction as they reacted with calves sera infected with toxocariasis. The proposed structure of GlcNAc fraction was Ser-Meth-Arg-O-methylated GlcNAc.

Conclusion: GlcNAc-rich fraction of T. vitulorum can be successfully utilized in the diagnosis of calves' toxocariasis.

Keywords: affinity chromatography, calves toxocariasis, Con A, enzyme-linked immunosorbent assay, mass spectrometric analysis, N-acetylglucosamine.

Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia

Research (Published online: 20-02-2019)

15. Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia

Michael H. Wibowo, Teridah E. Ginting and Widya Asmara

Veterinary World, 12(2): 277-287

ABSTRACT

Background and Aim: Existing data on the characteristics of infectious bronchitis virus (IBV) gathered throughout Indonesia have been recognized to indicate variants similar to globally distributed vaccine strains. Despite past and current intensive vaccination programs, IBV infections in the country's poultry industry have not been effectively controlled. Therefore, this study aimed to investigate the genotype of several isolates based on partial S1 gene sequences. In particular, the investigation is directed to focus on layer chickens in actively vaccinated farms indicating IBV symptoms.

Materials and Methods: Samples were isolated from ten different layer chicken flocks experiencing respiratory problem, drops in egg production, and a "penguin-like" stance, which were collected from commercial poultry farms in Central Java and Yogyakarta regions, Indonesia, within the periods of 2012-2018. Fragment of the S1 gene of IBV sampled from actively vaccinated commercial poultry farms was amplified using primer 5'-aca tgg taa ttt ttc aga tgg-3' (forward) and 5'-cag att gct tac aac cac c-3' (reverse) with the length of polymerase chain reaction (PCR) product at 383 bp. The sequence of samples was then compared with the sequence of reference S1 gene nucleotides of IBV from NCBI GenBank database. The amino acid analysis and multiple alignment sequence were conducted using Mega X.

Results: During necropsy, enlargement of the oviduct and swollen kidney were observed. Reverse transcription-PCR diagnosis of their 383 bp S1 gene showed that all samples were IBV positive. Phylogenetic analysis of the S1 gene discovered seven samples to be clustered as 4/91-like strains. Meanwhile, the remaining three samples were grouped in QX-like strain cluster.

Conclusion: This study is a pioneering report providing molecular evidence of pathogenic QX-like and 4/91-like strains circulating in Indonesia. Findings discovered, in this study, strongly suggested the importance of improving protections by available IBV vaccines through updated circulating strain clusters. It is critical to ensure the delivery of an effective control measurement of and vaccination protocols against IBV infections in the country's commercial poultry industry in particular and worldwide in general.

Keywords: avian infectious bronchitis virus, Indonesia, nephropathogenic, QX strain, 4/91 strain.

Stimulation of non-specific immunity, gene expression, and disease resistance in Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758), by the methanolic extract of the marine macroalga, Caulerpa scalpelliformis

Research (Published online: 16-02-2019)

14. Stimulation of non-specific immunity, gene expression, and disease resistance in Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758), by the methanolic extract of the marine macroalga, Caulerpa scalpelliformis

Omita Yengkhom, Konda Subramanian Shalini, P. A. Subramani and R. Dinakaran Michael

Veterinary World, 12(2): 271-276

Aim: The objective of the present study was to test the immunostimulating potential of marine macroalga, Caulerpa scalpelliformis, in terms of non-specific immune responses, gene expression, and disease resistance of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758).

Materials and Methods: O. niloticus was injected intraperitoneally with three different doses of methanol extract of C. scalpelliformis (CSME) (2 mg/kg, 20 mg/kg, or 200 mg/kg body weight), or MacroGardTM(commercial immunostimulant, positive control, and 20 mg/kg body weight), or distilled water (untreated control). In one set of fish, 5 days post-injection, serum lysozyme, myeloperoxidase, and antiprotease activities were assayed. 24 h after injection, gene expression was analyzed in a separate set of fish. To another set of fish, 1 week post-administration of the products, fish were challenged with lethal dose 50 (LD50) dose of a live virulent pathogen, Aeromonas hydrophila and subsequent resistance to it was noted in terms of cumulative percent mortality.

Results: CSME increased serum lysozyme, myeloperoxidase, and antiprotease activities. There was an increase in the expression of lysozyme gene in the spleen of treated fish. Mid dose of CSME caused the minimum mortality of 10% (consequent relative percentage survival = 73) which is comparable to that of the positive control.

Conclusion: CSME is considered to have the potential to be developed into an immunostimulant for finfish aquaculture.

Keywords: Aeromonas hydrophila, Caulerpa scalpelliformis, immunostimulant, macroalga, Oreochromis niloticus.

A preliminary molecular survey of Babesia divergens and first evidence of Theileria annulata in cattle from Saudi Arabia

Research (Published online: 15-02-2019)

13. A preliminary molecular survey of Babesia divergens and first evidence of Theileria annulata in cattle from Saudi Arabia

Mohamed W. Ghafar and Sayed A. M. Amer

Veterinary World, 12(2): 266-270

ABSTRACT

Background and Aim: Babesia divergens causes human babesiosis in Europe where the parasite utilizes cattle as animal reservoir and Ixodes ricinus as tick vector. Importation of infected animals and passive carriage of infected ticks through migratory birds can lead to tick/pathogen geographic expansion and emergence of diseases in naive land. Given the information that Saudi Arabia imports cattle from the European countries and that two global bird flyways pass through the country geographic coordinates, we speculate that B. divergens might be introduced into the Kingdom. Therefore, the aim of this preliminary study was to molecularly detect and characterize B. divergens and other piroplasms (including Theileria spp.) in cattle from Taif district, Kingdom of Saudi Arabia.

Materials and Methods: Blood samples from 20 cattle residing Taif district were collected, and polymerase chain reaction tested using wide and species-specific primers. Amplicons from a positive genus-wide reaction were purified, sequenced, and analyzed. Phylogenetic trees were constructed, and similarity to existing GenBank zoonotic piroplasms was also assessed.

Results: All samples were negative for B. divergens, and only one sample proved positive for Theileria annulata in a wide reaction. Phylogeny clustered our strain with T. annulata from Spanish dog and another one detected in a cow from France. BLAST analysis showed genetic distance from zoonotic piroplasms with identity ranged from 88% to 91%.

Conclusion: Although B. divergens was not detected, we are not able to rule out or affirm the existence of the pathogen in the country. On the other hand, identifying T. annulata strain with a southern European origin strongly supports our speculation that bovine zoonotic Babesia might be introduced into KSA. This study is not only the first molecular survey of B. divergens but also the first report of the molecular identity of T. annulata in Saudi Arabia. A national-wide bovine and tick surveillance are needed to further prove our speculation.

Keywords: Babesia divergens, cattle, molecular, Saudi Arabia, Theileria annulata.