Evaluation of antioxidant, diuretic, and wound healing effect of Tulkarm honey and its effect on kidney function in rats

Research (Published online: 25-10-2018)

21. Evaluation of antioxidant, diuretic, and wound healing effect of Tulkarm honey and its effect on kidney function in rats

Hamada Imtara, Noori Al-Waili, Meryem Bakour, Wail Al-Waili and Badiaa Lyoussi

Veterinary World, 11(10): 1491-1499

ABSTRACT

Aim: The composition and activity of honey depend on its floral origin. Honey collected from Tulkarm was evaluated for physicochemical property and antioxidant content as well as a diuretic and wound healing activity. Its effect on kidney function was evaluated and compared with furosemide.

Materials and Methods: Honey was collected in Tulkarm, Palestine, and its phenol, flavones, and flavonol content were assessed. The antioxidant activity was determined with the use of colorimetric assays, 1,1-diphenyl-2-picrylhydrazyl, ferric reducing antioxidant power, and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). Two sets of experiments were conducted. First experiment: 18 rats were used for the evaluation of diuretic activity of honey. The rats received either honey or furosemide. Renal function test, uric acid, and serum and urine electrolytes assay were performed. Second experiment: 18 male mice were used to evaluate the wound healing property of honey. Wounds were created on mice skin and treated daily with honey or Madecassol. Measurements of wounds were performed over a period of 12 days.

Results: The physical and chemical parameters of Tulkarm honey are within the limits of the European legislation and fulfilling the criteria described in the standard codex for honey. It contains antioxidant compounds and shows antioxidant activity. Oral honey increased creatinine clearance and urine volume, sodium, and chloride without causing hypokalemia or affecting blood urea, uric acid, or serum creatinine level. The diuretic activity of furosemide was associated with hypokalemia. Topical honey application enhanced wound closure when compared with the Madecassol application.

Conclusion: The study is the first to report that honey collected from Tulkarm has a considerable diuretic effect without affecting serum electrolytes or kidney function test and exhibits strong antioxidant activity and wound healing property.

Keywords: furosemide, honey, kidney, Madecassol, wounds.

An analysis of common foodborne parasitic zoonoses in slaughtered sheep and cattle in Tehran, Iran, during 2015-2018

Research (Published online: 24-10-2018)

20. An analysis of common foodborne parasitic zoonoses in slaughtered sheep and cattle in Tehran, Iran, during 2015-2018

Ali Pezeshki, Hadi Aminfar and Majid Aminzare

Veterinary World, 11(10): 1486-1490

ABSTRACT

Background and Aim: Cystic echinococcosis, Echinococcus granulosus, and liver flukes, such as Fasciola spp. and Dicrocoelium dendriticum, are important parasitic zoonoses, where they able to cause significant veterinary, medical, and economic problems. The present study was carried out to obtain the updated knowledge on the frequency of hydatidosis, fasciolosis, and dicrocoeliosis in the slaughtered sheep and cattle.

Materials and Methods: Information were collected from meat inspection records using systematically visual inspection, palpation, and incision of the visceral organs in the industrial abattoir in Tehran, the capital of Iran, between February 1, 2015, and January 31, 2018. For an analysis of the data, SPSS version 16 was applied.

Results: The hydatidosis infection in sheep and cattle was 2.48% and 2.25%, respectively. With respect to liver flukes, 0.62% and 0.25% sheep and cattle were infected by Fasciola spp., respectively; furthermore, 2.86% sheep and 0.79% cattle were positive for D. dendriticum.

Conclusion: The findings will provide considerable awareness for the future monitoring and control of these potentially important infections.

Keywords: dicrocoeliasis, fascioliasis, hydatidosis, sheep and cattle, slaughterhouse.

Nutritional potentialities of some tree leaves based on polyphenols and rumen in vitro gas production

Research (Published online: 23-10-2018)

19. Nutritional potentialities of some tree leaves based on polyphenols and rumen in vitro gas production

K. S. Giridhar, T. M. Prabhu, K. Chandrapal Singh, V. Nagabhushan, T. Thirumalesh, Y. B. Rajeshwari and B. C. Umashankar

Veterinary World, 11(10): 1479-1485

Aim: The study was conducted to evaluate eight tree leaves based on polyphenolic content and rumen in vitro incubation and gas production technique (RIVIGPT) for their nutritive potentiality.

Materials and Methods: Eight selected tree leaves, namely Sesbania grandiflora, Melia dubia, Dillenia spp., Artocarpus heterophyllus, Commiphora caudata, Moringa oleifera, Leucaena leucocephala, and Acacia auriculiformis, were selected for proximate composition, forage fiber fractions, total phenolics (TPs), non-tannin phenols (NTPs), total tannins (TTs), condensed tannins (CTs), and hydrolysable tannins (HTs); RIVIGP with and without polyethylene glycol (PEG); and in vitro dry matter digestibility (IVDMD) (modified in vitro two stage) analysis was conducted. On the basis of RIVIGPT, the in vitro digestible organic matter (IVDOM) and dry matter intake (DMI) was calculated.

Results: Crude protein (CP) content of tree leaves ranged from 9.59 to 25.81%, neutral detergent fiber (NDF) 28.16 to 53.33%, acid detergent fiber (ADF) 21.26 to 41.7%, acid detergent lignin (ADL) 3.62 to 21.98%, TP 1.83 to 17.35%, TT 0.40 to 15.47%, and CTs 0.02 to 15.26%. IVDMD (%) was ranged from 64.95 to 88.12. The mean metabolizable energy (ME) (MJ/Kg) of tree leaves estimated with and without PEG was 7.75±0.56 and 8.75±0.39, in vitro gas production at 24 h (IVGP24) (ml) 31.06±4.14 and 37.09±2.64, initial gas production (a) (ml) 0.49±0.63 and 1.33±0.72, potential gas production (D) (ml) 38.74±4.27 and 43.79±2.44, rate of gas production (k) (h-1) 0.11±0.02 and 0.11±0.013, t1/2 (ml) 9.81±2.41 and 7.42±0.80, in vitro gas production at 96 h IVGP96 (ml) 39.50±4.430 and 45.14±2.65, the predicted IVDOM (%) 55.44±4.15 and 61.98±3.03, and DMI (g/Kg W0.75) 103.1±14.76 and 104.3±10.16, respectively. The addition of PEG showed an improvement in IVGP24, IVGP96, ME, predicted IVDOM, and predicted DMI. CP was positively correlated with ME, IVGP24, IVGP96, a+b, k (r=0.749, p<0.05), IVDMD, IVDOM, and DMI (r=0.838, p<0.05) and negatively correlated with a and t1/2. NDF, ADF, and ADL contents were negatively correlated with ME (r=0.899, p<0.05), IVGP24 (r=-0.867, p<0.05), IVGP96 (r=-0.858, p<0.05), a+b (p<0.05), k (r=-0.828, p<0.05), IVDMD, IVDOM (r=-0.853, p<0.05), and DMI and positively correlated with a and t1/2. TP, TT, and CT were negatively correlated with ME, IVGP, IVGP96, a+b, k, IVDMD, IVDOM, and DMI and positively correlated with a (r=0.808, p<0.05) and t1/2. ME (MJ/Kg) was positively correlated with IVGP24 (r=0.938, p<0.05), IVGP96 (r=0.875, p<0.05), a+b (r=0.813, p<0.05), k (r=0.731, p<0.05), IVDMD, IVDOM (r=0.985, p<0.05), and DMI (r=0.727, p<0.05) and negatively correlated with a and t1/2.

Conclusion: In the present study, the potentiality of tree leaves was assessed based on CP, ADF, ADL, TP, CT, IVGP, ME, IVDMD, predicted IVDOM, and predicted DMI. Based on this, it can be concluded that S. grandiflora, M. dubia, M. Oleifera, and L. leucocephala were graded as best; A. heterophyllus and C. caudata as moderate; and Dillenia spp. and A. auriculiformis as lowest potential ruminant feed.

Keywords: chemical composition, in vitro, in vitro dry matter digestibility, in vitro digestible organic matter, metabolizable energy, polyethylene glycol, rumen in vitro incubation and gas production, ruminants, tannins, tree leaves.

Structural uterine changes in postpartum endometritis in cows

Research (Published online: 22-10-2018)

18. Structural uterine changes in postpartum endometritis in cows

S. M. Suleymanov, B. V. Usha, Yu. A. Vatnikov, E. D. Sotnikova, Eu. V. Kulikov, V. I. Parshina, M. V. Bolshakova, M. U. Lyshko and E. V. Romanova

Veterinary World, 11(10): 1473-1478

ABSTRACT

Aim: The purpose of this work was to study the dynamics of structural manifestations of acute cases of postpartum endometritis in cows.

Materials and Methods: The light and electron microscopy methods were used when studying structural changes in the endometrium in case of postpartum endometritis in seven cows. Sections of endometrial specimens for light microscopy, 5-7 μm thick, were stained with hematoxylin and eosin and also by Van Gieson's. For electron microscopy, semi-thin sections were stained with Azur-2 in combination with basic fuchsin, as well as contrasting by lead citrate and uranyl acetate.

Results: As a result of the study, we have established the following: Necrobiosis of the epithelial layer of the mucosa, cellular infiltration with shaped elements of blood in the functional layer, swelling of the cells of the uterine gland, and single microbial cells on the surface of the mucosa. We have noted edema of the stroma of the functional layer of the endometrium, swelling of the epithelial layer of the endometrial mucosa, and swelling of fibroblastic and lymphoid cells. Ultrastructural changes in endometrial cells in case of acute postpartum endometritis in cows are accompanied by the destruction of microvilli on the apical surface of the epithelium, an abundance of coccal microflora on the surface of the epithelium, necrobiosis of epithelial cells, and partial edema of the nucleus, and cytoplasm of the histiocyte.

Conclusion: We had established that acute purulent-catarrhal dystrophic processes were observed in the structural organization of the endometrium. In the depth of catarrhal mucus on the surface of the endometrium, there was an abundance of bacterial flora, with diplococci being prevalent. In ultrastructural organization of the endometrium, we observed deep dystrophic and necrobiotic processes in the parenchyma and endometrial stroma, as well as exudative processes with a change in the integrity of the microcirculatory bed. Thus, to prevent an inflammatory process from turning into a latent form, it is necessary to detect acute postpartum endometritis promptly using diagnostic methods taking into account the obtained parameters of the dynamics of structural changes in the uterine tissues.

Keywords: cows, histological, macroscopic, postpartum endometritis, subclinical endometritis, ultrastructural changes of the endometrium.

Opportunities and challenges associated with fecal progesterone metabolite analysis

Review (Published online: 20-10-2018)

17. Opportunities and challenges associated with fecal progesterone metabolite analysis

Innocent Damudu Peter, Abd Wahid Haron, Faez Firdaus Abdullah Jesse, Mokrish Ajat, Mark Hiew Wen Han, Wan Nor Fitri, Muhammad Sanusi Yahaya and Mohammed Saad M. Alamaary

Veterinary World, 11(10): 1466-1472

ABSTRACT

Conventionally, plasma or milk progesterone evaluations are used to determine the reproductive status of female animals. Collection of such samples is often associated with difficulties of animal handling and restraint. Measurable quantities of progesterone metabolites are found in feces of animals. Their concentrations are known to be well correlated to plasma progesterone levels and are, therefore, used as non-invasive samples for assessing reproductive function in a wide range of animal species. Although the analysis of fecal progesterone metabolites has been widely accepted in many laboratories, several factors are known to affect the results from this valuable analytical technique. Some of these factors include storage/ transportation media for fecal samples, type of solvent that is used for extraction of progesterone metabolites from feces, and the type and sensitivity of an assaying technique employed. Although fecal progesterone metabolites analysis is associated with some difficulties, it can effectively be used to monitor reproductive function in a wide range of animal species. This review aims to highlight the usefulness of fecal progesterone metabolite analysis as a non-invasive technique in monitoring reproductive function in animals. The article mainly focuses on the many opportunities and challenges associated with this analytical technique.

Keywords: non-invasive methods, progesterone metabolite, progesterone, reproductive cycles.

Antibiotic Susceptibility profile of Staphylococcus aureus isolated from sausages in Meknes, Morocco

Research (Published online: 19-10-2018)

16. Antibiotic Susceptibility profile of Staphylococcus aureus isolated from sausages in Meknes, Morocco

Abdelaziz Ed-Dra, Fouzia Rhazi Filali, Aziz Bouymajane, Faouzia Benhallam, Abdellah El Allaoui, Abdellah Chaiba and Filippo Giarratana

Veterinary World, 11(10): 1459-1465

ABSTRACT

Background and Aim: Staphylococcus aureus is one of the most common causes of foodborne disease worldwide, due to the consumption of food contaminated by their toxins. This study aimed to determine the prevalence and the antimicrobial resistance of S. aureus isolated from sausages in Meknes city of Morocco.

Materials and Methods: A total of 156 samples (Beef sausages, Turkey sausages, and Artisanal sausages "Merguez") were collected from different shopping sites (butchery, supermarket, street vendors, and weekly market "Souk") and used for the isolation of S. aureus. All the isolated strains were tested for their antimicrobials resistance to 16 antibiotics.

Results: Our results showed the presence of S. aureus in 63 samples (40.38%). Furthermore, the antimicrobial resistance study showed that 84.13% of isolated S. aureus were resistant to streptomycin, 76.20% to tetracycline, 42.86% to ampicillin, 41.27% to doxycycline, 38.1% to penicillin G, and 19.05% to chloramphenicol with the presence of 25 different phenotypic profiles. However, all isolated strains were sensitive to oxacillin, cefoxitin, gentamicin, and vancomycin.

Conclusion: The findings of this study revealed consumption of sausages as a potential risk of foodborne poisonings because of its contamination with the multi-resistant strains of S. aureus. Moreover, this contamination is related to the season, sampling sites and the origin of the raw material.

Keywords: antimicrobial resistance, foodborne disease, infection, sausages, Staphylococcus aureus.

Occurrence of Escherichia coli carrying Shiga toxin-producing genes in buffaloes on smallholdings in Bangladesh

Research (Published online: 19-10-2018)

15. Occurrence of Escherichia coli carrying Shiga toxin-producing genes in buffaloes on smallholdings in Bangladesh

Mukta Das Gupta, Arup Sen and Ashutosh Das

Veterinary World, 11(10): 1454-1458

ABSTRACT

Background and Aim: Shiga toxin-producing Escherichia coli (STEC) has emerged as significant foodborne pathogens. Ruminants are the primary reservoir of the zoonotic STEC. In Bangladesh, previous studies reported the presence of STEC in cattle, goat, and sheep; however, there is little information about STEC carriage by buffaloes. This study aimed to determine the occurrence of STEC in healthy (absence of clinical signs and symptoms) buffaloes on smallholdings in Bangladesh and to assess the antimicrobial resistance pattern of identified STEC isolates.

Materials and Methods: A total of 100 rectal swab samples were obtained from randomly selected buffaloes on 40 smallholdings in Chittagong Division, Bangladesh. Samples were subjected to bacteriological screening to identify E. coli. All E. coli isolates were examined for the presence of the Shiga toxin-producing genes - Shiga toxin 1 (stx1) and Shiga toxin 2 (stx2) using polymerase chain reaction. The antimicrobial susceptibility of identified STEC isolates was tested using the disk diffusion method.

Results: Results show that 71 fecal samples were positive for E. coli in bacteriological screening. The proportion of buffaloes harboring STEC isolates was 11% (11/100) (95% confidence interval [CI] 6.1-18.8], of which 7% (7/100) (95% CI 3.2- 13.9) and 4% (4/100) (95% CI 1.2-10.2) carried stx1 and stx2 genes, respectively. Antibiogram revealed that 91% (10/11), 73% (8/11), 55% (6/11), and 55% (6/11) STEC isolates were resistant to tetracycline, sulfamethoxazole-trimethoprim, erythromycin, and ampicillin, respectively. In contrast, 91% (10/11) STEC isolates were sensitive to ciprofloxacin, chloramphenicol, and gentamicin, whereas 73% (8/11) isolates were sensitive to ceftriaxone.

Conclusion: This study highlights, for the first time, a significant proportion of fecal samples from healthy buffaloes on smallholdings in Bangladesh harboring antimicrobial-resistant STEC. Transmission of antimicrobial-resistant STEC from buffaloes to humans could pose an added risk to public health in rural Bangladesh.

Keywords: antimicrobial resistance, buffalo, Shiga toxin-producing Escherichia coli.