Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study)

Research (Published online: 19-07-2018)

13. Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study)

Sri Wigati Mardi Mulyani, Diah Savitri Ernawati, Eha Renwi Astuti and Fedik Abdul Rantam

Veterinary World, 11(7): 965-970

ABSTRACT

Aim: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro.

Materials and Methods: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).

Results: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).

Conclusion: Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.

Keywords: bone marrow stem cells, C-X-C chemokine receptor type 4, hypoxic preconditioning, mesenchymal stem cells, stromal cells derived factor-1.

Effect of dietary supplementation of cinnamon oil and sodium butyrate on carcass characteristics and meat quality of broiler chicken

Research (Published online: 18-07-2018)

12. Effect of dietary supplementation of cinnamon oil and sodium butyrate on carcass characteristics and meat quality of broiler chicken

Govindarajan Gomathi, Subramaniam Senthilkumar, Amirthalingam Natarajan, Ramasamy Amutha and Manika Ragavan Purushothaman

Veterinary World, 11(7): 959-964

ABSTRACT

Aim: An in vivo experiment was conducted to investigate the effect of supplementation of cinnamon oil (CO) and sodium butyrate on carcass characteristics and meat quality of broiler chicken compared with the antibiotic supplementation.

Materials and Methods: A biological experiment was carried out with 216-day-old Vencobb-400 broiler chicks randomly distributed to six experimental treatments with six replicates, each replicate containing six chicks with equal numbers of male and female chicks. The experimental diets were prepared with isocaloric and isonitrogenous basis. The experimental groups, namely control (T1), control with antibiotic (T2), control with CO at 250 mg/kg and coated sodium butyrate (CSB) either at 0.09 (T3) or 0.18% (T4), and control with CO at 500 mg/kg and CSB either at 0.09 (T5) or 0.18% (T6). The trial was carried out in deep litter pen for 35 days. The carcass characteristics such as ready to cooked yield, eviscerated weight, heart, liver, gizzard, giblet, and abdominal fat percent in slaughtered birds and meat quality properties such as pH, water-holding capacity (WHC), tyrosine, shear force, cooking loss, thiobarbituric acid, sensory characteristics, and muscle cholesterol in breast muscle samples were evaluated.

Results: The carcass characteristics such as ready-to-cook yield, eviscerated weight, and weight of heart, liver, gizzard, giblet, and abdominal fat as a percent of live body weight were not influenced by supplementation of CO and CSB at the levels attempted or by antibiotic supplementation in broilers. The pH, cooking loss, shear force and WHC of meat, appearance, flavor, texture, mouth coating, juiciness and overall acceptability of meat were not influenced by the supplementation of different levels of CO and CSB or by antibiotic supplementation but decreased meat cholesterol level in broilers.

Conclusion: The results indicated that the supplementation of CO and CSB in broiler diet did not alter the carcass characteristics and meat quality parameters except meat cholesterol content in broilers.

Keywords: antibiotic, broilers, carcass characteristics, cinnamon oil, coated sodium butyrate, meat quality.

Efficacy of acetamiprid and fipronil fly baits against the housefly (Musca domestica L.) under laboratory conditions

Research (Published online: 16-07-2018)

11. Efficacy of acetamiprid and fipronil fly baits against the housefly (Musca domestica L.) under laboratory conditions

Mikhail Alekseevich Levchenko, Elena Anatol'evna Silivanova, Ruzilya Khusanovna Bikinyaeva and Galina Fedorovna Balabanova

Veterinary World, 11(7): 953-958

ABSTRACT

Background: The housefly Musca domestica L. (Diptera: Muscidae) is permanent pests in livestock facilities. High fly density in livestock and poultry farms can increase the risks of economic loss and public health. Treatment with toxic baits is one of the methods for housefly control. However, development of resistance to insecticides makes it difficult to manage of flies. Anti-resistance strategies include the use of multiple pesticides with different modes of action.

Aim: This study was conducted to estimate the efficacy of neonicotinoid acetamiprid and phenylpyrazole fipronil, applied alone or in the mixture, against adults of M. domestica and to evaluate the efficacy of fly bait formulations containing acetamiprid and fipronil under laboratory conditions.

Materials and Methods: The adult flies, M. domestica of laboratory strain, were used in laboratory bioassays. The efficacy of acetamiprid and fipronil as technical substances, when applied alone and in the mixture, against adult flies was tested by no-choice feeding bioassays. The insecticidal efficacy of bait formulations (wet powder) with acetamiprid or fipronil or their mixture was tested against flies by choice feeding bioassays. The probit analysis was used to calculate lethal concentrations of insecticides, and the χ2 test was used to analyze the interaction between fipronil and acetamiprid in the mixture.

Results: Fipronil was more toxic to adults of M. domestica than acetamiprid in laboratory tests. Lethal concentrations for 50% mortality (95% confidence interval) of flies were 0.0159% (0.0124-0.0205) of acetamiprid and 0.000119% (0.000039- 0.0002640) of fipronil. The mixture containing fipronil at concentration 0.005% and acetamiprid at concentration 0.05% had the additive effect on fly mortality.

Conclusion: The results of laboratory feeding bioassays indicate that the mixture of fipronil and acetamiprid might have a potential to use in toxic bait formulations against houseflies.

Keywords: fly bait, housefly management, insecticide interaction, insecticide mixture.

Effectiveness of platelet-rich fibrin matrix treated with silver nanoparticles in fracture healing in rabbit model

Research (Published online: 16-07-2018)

10. Effectiveness of platelet-rich fibrin matrix treated with silver nanoparticles in fracture healing in rabbit model

Serwa Ibrahim Salih, Nadia H. Al-Falahi, Ali H. Saliem and Ahmed N. Abedsalih

Veterinary World, 11(7): 944-952

ABSTRACT

Aim: The current study was conducted to evaluate the effect of platelet-rich fibrin matrix (PRFM) treated with silver nanoparticles (AgNPs) on enhancing the healing of the experimentally induced bone gap in a rabbit model.

Materials and Methods: Twenty healthy male local rabbits aged between 6 and 8 months, their weights between 1.5 and 2 kg were used in this study and divided randomly into four equal groups, under general anesthesia (1 cm), bone gap was induced in the tibia bone to create a critical bone defect and leave it without any treatment in the first group (control group). While in the second group the bone gap was filled with PRFM; in the third group, the gap was filled with 0.3 ml AgNPs; and in the fourth group, the gap was filled with PRFM treated with AgNPs.

Results: There was no infection at the operation site in all experimental animals, and the radiograph images showed periosteal and endosteal reaction; the gaps were bridged faster in the fourth group as compared with the other groups. The histological examination showed lamellar bone with haversian canal completely filled the fracture gap and contact with old bone in the fourth group as compared to other groups.

Conclusion: Using a combination of PRFM and single nucleotide polymorphisms together gave better acceleration in the bone healing process than using each one of them separately.

Keywords: bone gap, healing, platelet-rich fibrin matrix, silver nanoparticles.

Angiogenesis activity of Jatropha curcas L. latex in cream formulation on wound healing in mice

Research (Published online: 15-07-2018)

9. Angiogenesis activity of Jatropha curcas L. latex in cream formulation on wound healing in mice

Ummu Balqis, Darmawi, Cut Dahlia Iskandar and Muhammad Nur Salim

Veterinary World, 11(7): 939-943

ABSTRACT

Aim: The aim of this research was to determine the angiogenesis activity of Jatropha curcas latex in cream formulation on CD34 immune expression during wound healing phase in mice skin.

Materials and Methods: Amount of 36 2-month-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. All mice were divided into four groups, namely the base cream as control group (A), sulfadiazine 0.1% cream (B), Jatropha curcaslatex cream 10% (C), and J. curcas latex cream 15% (D). All groups were treated entire surface of wound. All experiments were performed twice a day for 10 days. Experiments were terminated on days 3, 7, and 10, respectively. The wound healing was assayed in stained histological section in immunohistochemical of the wounds. The CD34 expression was investigated under a microscope.

Results: The results showed that the cream from 10% and 15% latex J. curcas revealed moderate immune reaction to CD34 on days 3 and 7 in wound healing of mice skin.

Conclusion: We concluded that the cream from 10% and 15% latex J. curcas has potential as angiogenesis activity in wound healing of mice skin.

Keywords: angiogenesis, CD34, Jatropha curcas latex cream, wound healing.

Molecular characterization of full fusion protein (F) of Newcastle disease virus genotype VIId isolated from Egypt during 2012-2016

Research (Published online: 15-07-2018)

8. Molecular characterization of full fusion protein (F) of Newcastle disease virus genotype VIId isolated from Egypt during 2012-2016

Karim M. Selim, Abdullah Selim, Abdelsatar Arafa, Hussein A. Hussein and Ahmed A. Elsanousi

Veterinary World, 11(7): 930-938

ABSTRACT

Aim: The aim of this work was to study the sequence F gene of Newcastle disease virus (NDV) in regard to pathotyping and genotyping and to study the evolution of this NDV in Egypt.

Materials and Methods: The present study was conducted using samples from seven suspected NDV flocks of vaccinated chickens during 2012-2016 from six governorates in Egypt. The NDV was successfully isolated from pathological specimens through inoculation in specific pathogen-free embryonated chicken eggs.

Results: Pathogenicity of the NDV isolates has been estimated through intracerebral pathogenicity index and ranged from 1.66 to 1.73 which indicates the velogenic type of NDV isolates. Pathotyping and genotyping of these isolates were done through sequencing of full-length F gene. Results indicated that the seven NDV isolates showed characteristic cleavage site motif (112RRQKRF117) for the velogenic strains of NDV. Phylogenetic analysis of the F gene clustered these isolates within Group I of genotype VIId within Israeli strains NDV/IS/2015, NDV-Ch/SD883, and most of the Middle East strains. Six of seven sequenced isolates have six potential N-linked glycosylation sites. The neutralization epitope on the five antigenic sites of fusion is conserved in all Egyptian strains of this study except NDV-KFR-B7-2012 which has a substitution at D 170 N in epitope A4. In all our strains, 10 cysteine residues are recorded, except one loss of cysteine at residue 370 in both NDV-EG-35-2014 and NDV-GHB-328F-2016.

Conclusion: All viruses in this study have 52 amino acid substitutions within fusion gene in compared with Lasota strain that reveals importance for its antigenic and structural function. The present work highlights the important need to sequence F gene of NDV genotype VIId to investigate the evolution of this NDV in Egypt.

Keywords: cleavage site, fusion gene, heptad repeat domains.

Seroprevalence of Neospora caninum in local Bali dog

Research (Published online: 14-07-2018)

7. Seroprevalence of Neospora caninum in local Bali dog

I Made Dwinata, Ida Bagus Made Oka, Kadek Karang Agustina and I Made Damriyasa

Veterinary World, 11(7): 926-929

Aim: The aim of this research was to identify the seroprevalence of Neospora caninum in local Bali dogs.

Materials and Methods: A total of 147 local Bali dog serum samples have been examined for antibodies of N. caninum using enzyme-linked immunosorbent assay test.

Results: The results confirmed that 5 (3.4%) local Bali dogs have antibody for N. caninum. There were no significant differences in seroprevalence of Neospora infection in local Bali dogs between different genders, ages, and take care methods (p>0.05).

Conclusion: The results provided evidence for the presence of N. caninum infection in local Bali dogs and thus the risk to Bali cattle and dog health.

Keywords: local Bali dog, Neospora caninum, seroprevalence.