Serotyping, antibiotic susceptibility, and virulence genes screening of Escherichia coli isolates obtained from diarrheic buffalo calves in Egyptian farms

Research (Published online: 11-07-2017)

10. Serotyping, antibiotic susceptibility, and virulence genes screening of Escherichia coli isolates obtained from diarrheic buffalo calves in Egyptian farms

Ashraf S. Hakim, Shimaa T. Omara, Sohier M. Syame and Ehab A. Fouad

Veterinary World, 10(7): 769-773

ABSTRACT

Aim: In Egypt as in many other countries, river water buffalo (Bubalus bubalis) is considered an important source of high-quality milk and meat supply. The objective of this study was to investigate serotypes, virulence genes, and antibiotic resistance determinants profiles of Escherichia coli isolated from buffalo at some places in Egypt; noticibly, this issue was not discussed in the country yet.

Materials and Methods: A number of 58 rectal samples were collected from diarrheic buffalo calves in different regions in Egypt, and bacteriological investigated for E. coli existence. The E. coli isolates were biochemically, serologicaly identified, tested for antibiotic susceptibility, and polymerase chain reaction (PCR) analyzed for the presence of antibiotic resistance determinants and virulence genes.

Results: Overall 14 isolates typed as E. coli (24.1%); 6 were belonged to serogroup O78 (10.3%), followed by O125 (4 isolates, 6.9%), then O158 (3 isolates, 5.2%) and one isolate O8 (1.7%), among them, there were 5 E. coli isolates showed a picture of hemolysis (35.7%). The isolates exhibited a high resistance to β lactams over 60%, followed by sulfa (50%) and aminoglucoside (42.8%) group, in the same time the isolates were sensitive to quinolone, trimethoprim-sulfamethoxazole, tetracycline (100%), and cephalosporine groups (71.4%). A multiplex PCR was applied to the 14 E. coli isolates revealed that all were carrying at least one gene, as 10 carried blaTEM (71.4%), 8 Sul1 (57.1%), and 6 aadB (42.8%), and 9 isolates could be considered multidrug resistant (MDR) by an incidence of 64.3%. A PCR survey was stratified for the most important E. coli virulence genes, and showed the presence of Shiga toxins in 9 isolates carried either one or the two Stx genes (64.3%), 5 isolates carried hylA gene (35.7%), and eae in 2 isolates only (14.3%), all isolates carried at least one virulence gene except two (85.7%).

Conclusion: The obtained data displayed that in Egypt, buffalo as well as other ruminants could be a potential source of MDR pathogenic E. coli variants which have a public health importance.

Keywords: antibiotic resistance determinants, buffalo, Egypt, Escherichia coli, virulence.

Effects of commonly used chemical fertilizers on development of free-living stages of Haemonchus contortus in experimentally infected pasture

Research (Published online: 10-07-2017)

9. Effects of commonly used chemical fertilizers on development of free-living stages of Haemonchus contortus in experimentally infected pasture

Tapas Kumar Roul, Mitra Rajan Panda, Bijayendranath Mohanty, Kautuk Kumar Sardar, Manaswini Dehuri, Ananta Hembram and Trilochan Mohapatra

Veterinary World, 10(7): 764-768

Abstract

Aim: The effects of N-P-K fertilizers in the form of urea, single super phosphate and muriate of potash on development of free-living stages of Haemonchus contortus were studied.

Materials and Methods: Five parasite free experimental plots of 1 mx1 m area, each of paddy leaves (15-day-old) and an equal number of Cynodon dactylon grass were infested with about 10x10⁴ eggs/ml phosphate buffer saline along with the application of the calculated amount of fertilizers solution. On the 10th day of posttreatment, the pasture was cut, processed, larvae recovered by Baermann method and counted, which was expressed as number of L₃ per kg dry matter (DM) of pasture.

Results: The average recovered population of L₃ of H. contortus per kg DM varied significantly (p<0.05) between the paddy leaves (5933.57±22.718) and Cynodon grass (4861.00±22.718). When different doses of chemical fertilizer and their impact on different pasture were analyzed for control (T-1, 0-0-0 kg/ha N-P-K), the mean L₃ recovery per kg DM of paddy (19512.7±50.80) was more than that of Cynodon grass (16540.9±50.80). Larvae recovery per kg DM for different pastures under treatment were in decreasing order as follows: T-2 of paddy (6981.33±50.80, 35.77%), T-2 of Cynodon (5545.38±50.80, 33.52%), T-3 of paddy (317378±50.80, 16.26%), and T-3 of Cynodon (2218.72±50.80, 13.41%) which showed significant difference (p<0.05) among the treatments. In T-4 (paddy) and T-5 (Cynodon), the average number of recovery of larvae was nil implying no significant variation (p>0.05).

Conclusion: This study shown that when N-P-K fertilizers administered at recommended level, significantly reduced larval translation of H. contortus minimizing pasture infectivity for the free range grazing animals.

Keywords: Haemonchus contortus, larva, N-P-K fertilizer, pasture.

Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against Haemonchus contortus in sheep

Research (Published online: 08-07-2017)

8. Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against Haemonchus contortus in sheep

Omnia M. Kandil, Khaled A. Abdelrahman, Hatem A. Shalaby, Seham H. M. Hendawy, Nadia M. T. Abu El Ezz, Somia A. Nassar and James E. Miller

Veterinary World, 10(7): 758-763

Abstract

Aim: The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of Haemonchus contortus for immunization to control sheep hemonchosis.

Materials and Methods: A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of H. contortus on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged.

Results: Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively.

Conclusion: Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis.

Keywords: antigen, Haemonchus contortus, immunization, larval, rHcp26/23, sheep.

Repair of segmental radial defect with autologous bone marrow aspirate and hydroxyapatite in rabbit radius: A clinical and radiographic evaluation

Research (Published online: 07-07-2017)

7. Repair of segmental radial defect with autologous bone marrow aspirate and hydroxyapatite in 

rabbit radius: A clinical and radiographic evaluation

Kalbaza Ahmed Yassine, Benchohra Mokhtar, Hemida Houari, Amara Karim, and Melizi Mohamed

Veterinary World, 10(7): 752-757

ABSTRACT

Aim: Finding an ideal bone substitute to treat large bone defects, delayed union and nonunions remain a challenge for orthopedic surgeons and researchers. Several studies have been conducted on bone regeneration; each has its own advantages and disadvantages. The aim of this study was to evaluate the effect of a combination of hydroxyapatite (HA) powder with autologous bone marrow (BM) aspirate on the repair of segmental radial defect in a rabbit model.

Materials and Methods: A total of 36 male and adult New Zealand rabbit with a mean weight of 2.25 kg were used in this study. Approximately, 5 mm defect was created in the mid-shaft of the radius to be filled with HA powder in the control group "HA" (n=18) and with a combination of HA powder and autologous BM aspirate in the test group "HA+BM" (n=18). Animals were observed daily for healing by inspection of the surgical site, and six rabbits of each group were sacrificed at 30, 60, and 90 post-operative days to perform a radiographic evaluation of defect site.

Results: Obtained results revealed a better and more rapid bone regeneration in the test group: Since the defect was rapidly and completely filled with mature bone tissue after 90 days.

Conclusion: Based on these findings, we could infer that adding a BM aspirate to HA is responsible of a better regeneration process leading to a complete filling of the defect.

Keywords: autologous bone marrow aspirate, bone regeneration, hydroxyapatite, rabbit, radial defect.

Noninvasive assessment of gastrointestinal parasites infection in freeranging wild herbivores and adjoining livestock of Panna Tiger Reserve, Madhya Pradesh, India

Research (Published online: 06-07-2017)

6. Noninvasive assessment of gastrointestinal parasites infection in freeranging wild herbivores and adjoining livestock of Panna Tiger Reserve, Madhya Pradesh, India

Abhay Sengar, A. B. Shrivastav, K. P. Singh and Amol Rokde

Veterinary World, 10(7): 748-751

ABSTRACT

Aim: This study was conducted to know the epidemiology of gastrointestinal parasites of free-ranging wild herbivores and adjoining livestock of Panna Tiger Reserve, Madhya Pradesh, India.

Materials and Methods: A total of 374 fecal samples from wild herbivores (Chital Axis axis - 123, Sambar Rusa unicolor - 94, Nilgai Boselaphus tragocamelus - 86, and Chinkara Gazella bennettii - 71) and 284 fecal samples of domestic herbivores (cattle - 118, buffalo - 78, and goat - 88) were collected from common grazing land and adjoining area of tiger reserve. Detailed coprological examination for the presence of parasitic eggs/oocysts by direct smear examination, standard sedimentation, and floatation techniques was performed.

Results: Fecal samples (n=374) of four different species of wild herbivores were screened. Out of which, 55.61% (n=208) were positive for parasitic infection. Among them, 13.10% (n=49) were positive for mixed parasitic infection of two or more parasite and 42.5% (n=159) were found positive for single parasitic infection. A total of 284 fecal samples of domestic animals were screened from adjoining areas of the tiger reserve. Out of which, 66.54% (n=189) were positive for parasitic infections, out of which 19.71% (n=56) were positive for mixed infection of two or more parasites, and 46.83% (n=133) were found positive for single parasitic infection.

Conclusion: Wild herbivores at Panna Tiger Reserve were exposed to parasites including some that are known to be pathogenic; majority of wild animals had mixed infection of Eimeria spp., Trichuris spp., Monieziaspp., Amphistome, Strongyloides spp., Balantidium spp., and Fasciola spp.

Keywords: free ranging wild herbivores, gastrointestinal parasites, Panna Tiger Reserve.

Anti-diarrhea activity of the aqueous root bark extract of Byrsocarpus coccineus on castor oil-induced diarrhea in Wistar rats

Research (Published online: 05-07-2017)

5. Anti-diarrhea activity of the aqueous root bark extract of Byrsocarpus coccineus on castor oil-induced diarrhea in Wistar rats

Sunday A. Ejeh, Patrick Onyeyili and Samson E. Abalaka

Veterinary World, 10(7): 743-747

doi: 10.14202/vetworld.2017.743-747

Abstract

Aim: The use of traditional medicine as an alternative source of cure for many ailments has played an important role in health care delivery in both developing and developed countries. Byrsocarpus coccineus Schum and Thonn (Connaraceae) is used in traditional medicine for treatment of various disease conditions, including diarrhea. The anti-diarrhea activity of the root bark aqueous extract of B. coccineus was investigated in this study.

Materials and Methods: Acute toxicity evaluation of the aqueous extract of B. coccineus root bark was performed in exposed rats. Diarrhea was induced in exposed rats with castor oil, and the effect of the extract on castor oil-induced gastrointestinal motility and enteropooling was consequently investigated.

Results: In the acute toxicity study, the extract caused no death in treated rats nor produced signs of delayed toxicity, even at 5000 mg/kg. The aqueous root bark extract of B. coccineus also decreased the distance travelled by activated charcoal in the gastrointestinal tract of treated rats when compared to control rats. Results of castor oil-induced enteropooling revealed slight reduction in the weight of intestinal contents of treated rats compared to control rats. There was significant (p<0.05) decrease in the frequency of defecation as well as in the number of unformed feces produced by castor oil-induced diarrhea at 100 mg/kg dose with 74.96% inhibition of defecation.

Conclusion: The study demonstrated the anti-diarrheic property of the aqueous extract of B. coccineus root bark as currently exploited in our traditional herbal therapy.

Keywords: Byrsocarpus coccineus, castor oil, diarrhea, enteropooling.

Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India

Research (Published online: 04-07-2017)

4. Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India

S. Banerjee, K. Batabyal, S. N. Joardar, D. P. Isore, S. Dey, I. Samanta, T. K. Samanta and S. Murmu

Veterinary World, 10(7): 738-742

ABSTRACT

Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of the dairy farmers, of which Pseudomonas aeruginosa is one of the causative agents. The study was aimed at a screening of suspected milk samples from different cattle farms of West Bengal for detection and confirmation of P. aeruginosa strains followed by their characterization.

Materials and Methods: Around 422 milk samples were screened from different dairy farms primarily by on-spot bromothymol blue (BTB) test and then in the lab by somatic cell counts (SCC) to finally consider 352 samples for detection of P. aeruginosa. Selective isolation and confirmation of the isolates were done using selective media, viz., cetrimide and Pseudomonas agar followed by confirmation by fluorescent technique. Molecular characterization of the strains was done by polymerase chain reaction for the presence of toxA (enterotoxin A, 352 bp) and exoS (exoenzyme S, 504 bp) genes.

Results: Approximately, 371 (87.9%) samples were positive in on-spot BTB test among which 352 (94.8%) samples revealed high SCC values (more than 3 lakh cells/ml) showing infection when screened. Among these, 23 (6.5%) samples yielded typical Pseudomonas sp. isolates out of which only 19 (5.4%) isolates were confirmed to be P. aeruginosa which showed characteristic blue-green fluorescence due to the presence of pigment pyoverdin under ultraviolet light. Out of these 19 isolates, 11 isolates were positive for toxA, 6 isolates for exoS, and 2 for both these pathogenic genes.

Conclusion: Approximately, 5.4% cases of bovine subclinical mastitis infections in South Bengal were associated with P. aeruginosa which possess pathogenic genes such as toxA (63.2%) and exoS (36.8%).

Keywords: bovines, characterization, exoS, Pseudomonas aeruginosa, subclinical mastitis, toxA.