Molecular identification and histopathological study of natural Streptococcus agalactiae infection in hybrid tilapia (Oreochromis niloticus)

Research (Published online: 24-01-2017)

16. Molecular identification and histopathological study of natural Streptococcus agalactiae infection in hybrid tilapia (Oreochromis niloticus) - Laith Abdul Razzak, Mohd Azmi Ambak, Marina Hassan, Shahreza Md. Sheriff, Musa Nadirah, Ahmad Shuhaimi Draman, Wahidah Wahab, Wan Wan Ibrahim Nurhafizah, Syafiqah Aznan Alia, Amina Jabar and Musa Najiah

Veterinary World, 10(1): 101-111

   doi: 10.14202/vetworld.2017.101-111

Abstract

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Aim: The main objective of this study was to emphasize on histopathological examinations and molecular identification of Streptococcus agalactiae isolated from natural infections in hybrid tilapia (Oreochromis niloticus) in Temerloh Pahang, Malaysia, as well as to determine the susceptibility of the pathogen strains to various currently available antimicrobial agents.

Materials and Methods: The diseased fishes were observed for variable clinical signs including fin hemorrhages, alterations in behavior associated with erratic swimming, exophthalmia, and mortality. Tissue samples from the eyes, brain, kidney, liver, and spleen were taken for bacterial isolation. Identification of S. agalactiae was screened by biochemical methods and confirmed by VITEK 2 and 16S rRNA gene sequencing. The antibiogram profiling of the isolate was tested against 18 standard antibiotics included nitrofurantoin, flumequine, florfenicol, amoxylin, doxycycline, oleandomycin, tetracycline, ampicillin, lincomycin, colistin sulfate, oxolinic acid, novobiocin, spiramycin, erythromycin, fosfomycin, neomycin, gentamycin, and polymyxin B. The histopathological analysis of eyes, brain, liver, kidney, and spleen was observed for abnormalities related to S. agalactiae infection.

Results: The suspected colonies of S. agalactiae identified by biochemical methods was observed as Gram-positive chained cocci, β-hemolytic, and non-motile. The isolate was confirmed as S. agalactiae by VITEK 2 (99% similarity), reconfirmed by 16S rRNA gene sequencing (99% similarity) and deposited in GenBank with accession no. KT869025. The isolate was observed to be resistance to neomycin and gentamicin. The most consistent gross findings were marked hemorrhages, erosions of caudal fin, and exophthalmos. Microscopic examination confirmed the presence of marked congestion and infiltration of inflammatory cell in the eye, brain, kidney, liver, and spleen. Eye samples showed damage of the lens capsule, hyperemic an d hemorrhagic choroid tissue, and retina hyperplasia accompanied with edema. Brain samples showed perivascular and pericellular edema and hemorrhages of the meninges. Kidney samples showed hemorrhage and thrombosis in the glomeruli and tubules along with atrophy in hematopoietic tissue. Liver samples showed congestion of the sinusoids and blood vessel, thrombosis of portal blood vessel, and vacuolar (fatty) degeneration of hepatocytes. Spleen samples showed large thrombus in the splenic blood vessel, multifocal hemosiderin deposition, congestion of blood vessels, and multifocal infiltration of macrophages.

Conclusion: Therefore, it can be concluded that pathological changes in tissues and organs of fish occur proportionally to the pathogen invasion, and because of their high resistance, neomycin and gentamicin utilization in the prophylaxis or treatment of S. agalactiae infection should be avoided.

Keywords: 16S rDNA, antibiotic resistance, aquaculture, histopathological examination, polymerase chain reaction, Streptococcus agalactiae.

Preparation of goat and rabbit anti-camel immunoglobulin G whole molecule labeled with horseradish peroxidase

Research (Published online: 23-01-2017)

15. Preparation of goat and rabbit anti-camel immunoglobulin G whole molecule labeled with horseradish peroxidase - Eman Hussein Abdel-Rahman, Jakeen Kamal El-Jakee, Mahmoud Essam Hatem, Nagwa Sayed Ata and Ehab Ali Fouad

Veterinary World, 10(1): 92-100

   doi: 10.14202/vetworld.2017.92-100

Abstract

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Aim: As the labeled anti-camel immunoglobulins (Igs) with enzymes for enzyme-linked immunosorbent assay (ELISA) are unavailable in the Egyptian market, the present investigation was directed for developing local labeled anti-camel IgG with horseradish peroxidase (HRP) to save hard curacy.

Materials and Methods: For purification of camel IgG whole molecule, camel sera was preliminary precipitated with 50% saturated ammonium sulfate and dialyzed against 15 mM phosphate-buffered saline pH 7.2 then concentrated. This preparation was further purified by protein A sepharose affinity column chromatography. The purity of the eluted camel IgG was tested by sodium dodecyl sulfate polyacrylamide gel electrophoresi. Anti-camel IgG was prepared by immunization of goats and rabbits separately, with purified camel IgG. The anti-camel IgG was purified by protein A sepharose affinity column chromatography. Whole molecule anti-camel IgG was conjugated with HRP using glutraldehyde based assay. Sensitivity and specificity of prepared conjugated secondary antibodies were detected using positive and negative camel serum samples reacted with different antigens in ELISA, respectively. The potency of prepared conjugated antibodies was evaluated compared with protein A HRP. The stability of the conjugate at −20°C during 1 year was assessed by ELISA.

Results: The electrophoretic profile of camel IgG showed four bands of molecular weight 63, 52, 40 and 33 kDa. The recorded sensitivity and specificity of the product are 100%. Its potency is also 100% compared to 58-75% of commercial protein A HRP. The conjugates are stable for 1 year at −20°C as proved by ELISA.

Conclusion: Collectively, this study introduces goat and rabbit anti-camel IgG whole molecules with simple, inexpensive method, with 100% sensitivity, 100% specificity and stability up to 1 year at −20°C. The important facet of the current study is saving hard curacy. Future investigations are necessary for preparation of IgG subclasses.

Keywords: anti-camel immunoglobulin G, Camelus dromedarius, conjugation, horseradish peroxidase, purification.

Social engineering of societal knowledge in livestock science: Can we be more empathetic?

Review (Published online: 23-01-2017)

14. Social engineering of societal knowledge in livestock science: Can we be more empathetic? - R. K. Ravikumar, Devesh Thakur, Hardev Choudhary, Vivek Kumar, Amol S. Kinhekar, Tushar Garg, K. Ponnusamy, G. R. Bhojne, Vasanth M. Shetty and Vipin Kumar

Veterinary World, 10(1): 86-91

   doi: 10.14202/vetworld.2017.86-91

Abstract

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Questions are raised in effective utilization of farmer’s wisdom by communities in their farming. Planners support to livelihood emphasize mostly of inputs from outside and not setting up sustainable goals. Formal institutions and planners of program are finding constraints and sceptical in wider dissemination of indigenous knowledge research system (IKRS). This is in spite of evidence that considerable number of farmer’s in livestock sector depends on IKRS. In this context, it is pertinent to showcase dissemination potential of these knowledge system(s) in larger geographical areas. The review illustrates different challenges encountered while control of livestock ailments like ectoparasite infestation through IKRS. Several times, it was opinioned to provide or share IKRS to thwart ailments in a specific region. This is interesting as it was narrated how formal system is unable to recognize farmer’s problem and challenges in integrating these sustainable practices. It has to be noted that disseminating activities seldom takes into account the experimental potential of farmers. This review paper articulates various evidences generated in enhancing diffusion thereby dissemination of IKRS. The nature of support extended by IKRS in entrepreneurial activity of smallholder farming units did not get adequate recognition. There needs to be minimum standard protocol in deriving benefit from such low-cost alternative technologies. This will enrich incremental innovation activities as per location specific need and provide scope for wider dissemination.

Keywords: health, indigenous knowledge, innovation, institution, livestock, technology.

Gastrointestinal parasitism of goats in hilly region of Meghalaya, India

Research (Published online: 19-01-2017)

13. Gastrointestinal parasitism of goats in hilly region of Meghalaya, India - Meena Das, R. Laha, A. Goswami and A. Sen

Veterinary World, 10(1): 81-85

   doi: 10.14202/vetworld.2017.81-85

Abstract

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Aim: The aim of this study was to determine the prevalence of gastrointestinal (GI) parasitic infections in goats of hilly region of Meghalaya.

Materials and Methods: A total of 834 fecal samples of goats were screened for 1 year (2014-2015) using flotation techniques.

Results: The overall prevalence of GI parasitic infections in goats was 28.65%. Season-wise highest infections were recorded during rainy season (34.92%) followed by cool (26.87%), hot (26.62%), and cold (20.39%) seasons. Helminths and protozoa infections were recorded in 63.60% and 23.02% animals, respectively. Among the helminths, Strongyle spp. (32.63%) was recorded highest followed by Trichuris spp. (12.55%), Moniezia spp. (10.04%), and Trichuris spp. (8.36%). Among protozoa, only Eimeria spp. was detected. Seven different species of Eimeria spp. were identified, viz., Eimeria christenseni, Eimeria hirci, Eimeria caprina, Eimeria jolchijevi, Eimeria ninakohlyakimovae, Eimeria arloingi, and Eimeria kocharii for the first time from Meghalaya. Maximum egg per gram and oocyst per gram of feces were recorded in the month of August (932.4) and September (674.05), respectively. Mixed infections were recorded in 13.38% samples. Coproculture of goat fecal samples revealed the presence of Haemonchus contortus (72.16%), Oesophagostomum spp. (14.41%), Strongyloides spp. (8.91%), and Trichostrongylus spp. (4.50%) larvae.

Conclusion: This study indicates that GI helminths and protozoa infections are prevalent in goats of this hilly region of Meghalaya, throughout the year and highly prevalent during rainy season.

Keywords: gastrointestinal parasites, goat, helminths, Meghalaya, protozoa.

Protective effect of rutin in comparison to silymarin against induced hepatotoxicity in rats

Research (Published online: 19-01-2017)

12. Protective effect of rutin in comparison to silymarin against induced hepatotoxicity in rats - M. Kasi Reddy, A. Gopala Reddy, B. Kala Kumar, D. Madhuri, G. Boobalan and M. Anudeep Reddy

Veterinary World, 10(1): 74-80

   doi: 10.14202/vetworld.2017.74-80

Abstract

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Aim: The aim of this study is to evaluate the hepatoprotective effect of rutin (RTN) in comparison to silymarin (SLM) against acetaminophen (APAP)-induced hepatotoxicity in rats.

Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.

Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.

Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.

Keywords: acetaminophen, hepatotoxicity, rutin, silymarin.

Influence of training and competitive sessions on peripheral β-endorphin levels in training show jumping horses

Research (Published online: 18-01-2017)

11. Influence of training and competitive sessions on peripheral β-endorphin levels in training show jumping horses - Cristina Cravana, P. Medica, G. Ragonese and E. Fazio

Veterinary World, 10(1): 67-73

   doi: 10.14202/vetworld.2017.67-73

Abstract

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Aim: To investigate the effects of training sessions on circulating β-endorphin changes in sport horses before and after competition and to ascertain whether competition would affect this response.

Materials and Methods: A total of 24 trained jumping horses were randomly assigned to one of two training groups: Group A (competing) and Group B (not competing). To determined plasma β-endorphin concentrations, two pre- and postcompetition training weeks at aerobic workout and two competitive show jumping event days at anaerobic workout were measured before, 5 and 30 min after exercise. Exercise intensity is described using lactate concentrations and heart rate. The circuit design, intensity, and duration of training sessions were the same for both groups.

Results: In Group A, one-way analysis of variance for repeated measures (RM-ANOVA) showed significant effects of exercise on β-endorphin changes (F=14.41; p<0.001), only in the post-competition training sessions, while in Group B showed no significant effects. Two-way RM-ANOVA showed, after post-competition training sessions, a significant difference between Group A and Group B (F=6.235; p=0.023), with higher β-endorphin changes in Group A, compared to Group B. During the competitive show jumping sessions, one-way RM ANOVA showed significant effects of exercise on β-endorphin changes (F=51.10; p<0.001). The statistical analysis, in Group A, showed a significant difference between postcompetition training and competitive exercise (F=6.32; p=0.024) with higher β-endorphin values in competitive sessions compared to those of post-competition training.

Conclusion: Lactate concentrations seem to be the main factors being correlated with the raise of β-endorphin during anaerobic exercise of competitive events. Exercise of low intensity, as well as that one of training sessions, does not appear to stimulate a significant increased release of β-endorphin and it may depend on the duration of the exercise program. Moreover, the responses during exercise in the course of post-competition training sessions seem to be significantly different from those the pre-competition training. These data show that the preliminary competitive stress induced additional significant changes of β-endorphin pattern. It would reflect the need of a long-lasting modulation of fatigue and pain perception related to the effect of an additional physical and mental effort for the consecutive competitive and training sessions.

Keywords: β-endorphin, competition, horse, show jumping, training.

Prevalence of gastrointestinal parasitism in small ruminants in western zone of Punjab, India

Research (Published online: 17-01-2017)

10. Prevalence of gastrointestinal parasitism in small ruminants in western zone of Punjab, India - E. Singh, P. Kaur, L. D. Singla and

M. S. Bal

Veterinary World, 10(1): 61-66

   doi: 10.14202/vetworld.2017.61-66

Abstract

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Aim: The aim of this study was to explore the prevalence of gastrointestinal parasitism in small ruminants in relation to various risk factors in the western zone of Punjab.

Materials and Methods: During the study, 603 fecal samples (391 of sheep and 212 of goats) were examined qualitatively by floatation and sedimentation techniques, and quantitatively by McMaster technique.

Results: Out of the 603 fecal (391 sheep and 212 goats) samples examined, 501 were found positive for endoparasitic infection with an overall prevalence of 83.08%, consisting of 85.16% and 79.24% in sheep and goats, respectively. Egg per gram in sheep was apparently more 1441.88±77.72 than goats 1168.57±78.31. The associated risk factors with the prevalence of gastrointestinal tract (GIT) parasites showed that females (85.97%) were significantly more susceptible than males (69.23%). Age wise the adults (>6 months) were significantly more prone to parasitic infection as compared to young ones (<6 months). Seasonal variation was recorded throughout the year and was significantly highest during monsoon (90.10%), followed by winter (83.84%) and summer (78.35%).

Conclusion: The study revealed an overall prevalence of 83.08% of GIT parasitic infections in small ruminants constituting 85.16% in sheep and 79.24% in goats in the western zone of Punjab. The most relevant risk factors for the prevalence of gastrointestinal parasitism in ruminants were sex, age, and season.

Keywords: gastrointestinal parasitism, goat, prevalence, Punjab, sheep, western zone.

Veterinary World reviewer acknowledgment 2016

Reviewer Acknowledgment (Published online: 17-01-2017)

9. Veterinary World reviewer acknowledgment 2016 - A. V. Sherasiya and Nazir

Veterinary World, 10(1): 56-60

   doi: 10.14202/vetworld.2017.56-60

Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage

Research (Published online: 13-01-2017)

8. Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage - Sharadindu Shil, R. S. Joshi, C. G. Joshi, A. K. Patel, Ravi K. Shah, Namrata Patel, Subhash J. Jakhesara, Sumana Kundu, Bhaskar Reddy, P. G. Koringa and D. N. Rank

Veterinary World, 10(1): 38-55

   doi: 10.14202/vetworld.2017.38-55

Abstract

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Aim: Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed in Bos indicus affecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene expression in between horn’s SCC tissue and its early passage primary culture using next generation sequencing was the aim of this study.

Materials and Methods: Whole transcriptome sequencing of horn’s SCC tissue and its early passage cells using Ion Torrent PGM were done. Comparative expression and analysis of different genes and pathways related to cancer and biological processes associated with malignancy, proliferating capacity, differentiation, apoptosis, senescence, adhesion, cohesion, migration, invasion, angiogenesis, and metabolic pathways were identified.

Results: Up-regulated genes in SCC of horn’s early passage cells were involved in transporter activity, catalytic activity, nucleic acid binding transcription factor activity, biogenesis, cellular processes, biological regulation and localization and the down-regulated genes mainly were involved in focal adhesion, extracellular matrix receptor interaction and spliceosome activity.

Conclusion: The experiment revealed similar transcriptomic nature of horn’s SCC tissue and its early passage cells.

Keywords: cummerbund, gene ontology, primary culture, RNA-sequencing, squamous cell carcinoma of horn, transcriptome profiling.

Incidence and virulence characteristics of Aeromonas spp. in fish

Research (Published online: 12-01-2017)

7. Incidence and virulence characteristics of Aeromonas spp. in fish - Ashraf M. Abd-El-Malek

Veterinary World, 10(1): 34-37

   doi: 10.14202/vetworld.2017.34-37

Abstract

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Aim: This study was conducted to evaluate the presence of Aeromonas spp. in raw and ready-to-eat (RTE) fish commonly consumed in Assiut city, Egypt, and to determine virulence factors due to they play a key role in their pathogenicity.

Materials and Methods: A total of 125 samples of raw and RTE fish samples were taken from different fish markets and fish restaurants in Assiut Governorate and screened for the presence of Aeromonas spp. by enrichment on tryptic soy broth then incubated at 30°C for 24 h. Plating unto the sterile Petri dishes containing Aeromonas agar base to which Aeromonas selective supplement was added. The plates were incubated at 37°C for 24 h. Presumptive Aeromonas colonies were biochemically confirmed and analyzed for pathogenicity by hemolysin production, protease, and lipase detection.

Results: The results indicated that raw fish were contaminated with Aeromonas spp. (40% in wild and 36% in cultured Nile tilapia). Regarding RTE, Aeromonas spp. could be isolated with the percentage of 16%, 28% and 20% in fried Bolti, grilled Bolti and fried Bayad, respectively. Out of 35 isolates obtained, 22 were categorized as Aeromonas hydrophila, 12 were classified as Aeromonas sobria and Aeromonas caviae were found in only one isolate. The virulence factors of Aeromonas spp. were detected and the results showed that all isolates produced of hemolysin (91.4%), protease (77.1%), and lipase enzyme (17.1%).

Conclusion: This study indicates that the presence of A. hydrophila with virulence potential in fresh and RTE fish may be a major threat to public health.

Keywords: Aeromonas hydrophila, fish, hemolysin, lipase, protease enzyme, public health.