Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis

Research (Published online: 28-04-2016)

15. Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis - Taruna Bhati, Prerna Nathawat, Sandeep Kumar Sharma, Rahul Yadav, Jyoti Bishnoi and Anil Kumar Kataria

Veterinary World, 9(4): 421-424

   doi: 10.14202/vetworld.2016.421-424

Abstract

---

Aim: The virulence-associated protein-A of Staphylococcus aureus, encoded by spa gene shows a variation in length in different strains. In this study, the spa gene variation in S. aureus strains was studied which were isolated from subclinical cases of bovine mastitis.

Materials and Methods: About 38 isolates of S. aureus were recovered from Holstein–Friesian (HF) crossbred (n=16) and Rathi cattle (n=22) with subclinical mastitis as per standard procedures, and these isolates were subjected to amplification of spa gene (X-region) by polymerase chain reaction and calculation of number of tandem repeats were done.

Results: Of the 16 isolates from H-F crossbred cattle, all with the exception of one isolate produced spa amplicon. Seven isolates produced amplicons of 200 bp, one produced 160 bp, and other seven produced spa amplicon of 150 bp with calculated number of 6, 5, and 4 repeats, respectively, whereas nine different types of amplicons were produced by 22 S. aureus isolates from Rathi cattle, viz., 280, 250, 240, 200, 190, 180, 170, 150, and 140 bp with 10, 8, 8, 6, 6, 6, 5, 4, and 4 repeats, respectively. One of the isolates from Rathi cattle produced two spa amplicons (150 and 190 bp).

Conclusion: A greater polymorphism was observed in the S. aureus isolates from Rathi cattle than from H-F crossbreds with subclinical mastitis.

Keywords: cattle, polymorphism, protein-A, spa gene, Staphylococcus aureus, subclinical mastitis.

In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage

Research (Published online: 28-04-2016)

14. In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage - Norisal Binti Nasai, Yusuf Abba, Faez Firdaus Jesse Abdullah, Murugaiyah Marimuthu, Abdulnasir Tijjani, Muhammad Abubakar Sadiq, Konto Mohammed, Eric Lim Teik Chung and Mohammed Ariff Bin Omar

Veterinary World, 9(4): 417-420

   doi: 10.14202/vetworld.2016.417-420

Abstract

---

Aim: Gastrointestinal helminthosis is a global problem in small ruminant production. Most parasites have developed resistance to commonly available anthelminthic compounds, and there is currently an increasing need for new compounds with more efficacies. This study evaluated the in vitro effects of ethanolic extract of Curcuma longa (EECL) as a biological nematicide against third stageHaemonchus larvae (L3) isolated from sheep.

Materials and Methods: Haemonchus L3 were cultured and harvested from the feces of naturally infected sheep. EECL was prepared and three concentrations; 50, 100, and 200 mg/mL were tested for their efficacies on Haemonchus L3. Levamisole at concentration 1.5 and 3 mg/mL were used as positive controls.

Results: EECL showed anthelmintic activity in a dose-dependent manner with 78% worm mortality within 24 h of exposure at the highest dose rate of 200 mg/mL. There was a 100% worm mortality rate after 2 h of levamisole (3 mg/mL) admisntration. However, there was a comparable larvicidal effect between when levamisole (1.5 mg/mL) and EECL (200 mg) were administered.

Conclusion: The study shows that EECL does exhibit good anthelmintic properties at 200 mg/mL which is comparable with levamisole at 1.5 mg/mL.

Keywords: Curcuma longa, ethanolic extract, Haemonchus, larvae 3, levamisole, strongyle.

Conjugation of ampicillin and enrofloxacin residues with bovine serum albumin and raising of polyclonal antibodies against them

Research (Published online: 26-04-2016)

13. Conjugation of ampicillin and enrofloxacin residues with bovine serum albumin and raising of polyclonal antibodies against them - B. Sampath Kumar, Vasili Ashok, P. Kalyani and G. Remya Nair

Veterinary World, 9(4): 410-416

   doi: 10.14202/vetworld.2016.410-416

Abstract

---

Aim: The aim of this study is to test the potency of bovine serum albumin (BSA) conjugated ampicillin (AMP) and enrofloxacin (ENR) antigens in eliciting an immune response in rats using indirect competitive enzyme-linked immunosorbent assay (icELISA).

Materials and Methods: AMP and ENR antibiotics were conjugated with BSA by carbodiimide reaction using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as a cross-linker. The successful conjugation was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Sprague-Dawley rats were immunized with the conjugates and blood samples were collected serially at 15 days time interval after first immunization plus first booster, second booster, third booster, and the fourth sampling was done 1½ month after the third booster. The antibody titres in the antisera of each antibiotic in all the four immunization cycles (ICs) were determined by an icELISA at various serum dilutions ranging from 1/100 to 1/6400.

Results: Analysis of antibiotic-BSA conjugates by sodium dodecyl sulfate polyacrylamide gel electrophoresis and coomassie blue staining revealed high molecular weight bands of 85 kDa and 74 kDa for AMP-BSA and ENR-BSA respectively when compared to 68 kDa band of BSA. Both the antibiotic conjugates elicited a good immune response in rats but comparatively the response was more with AMP-BSA conjugate than ENR-BSA conjugate. Maximum optical density 450 value of 2.577 was recorded for AMP-BSA antisera, and 1.723 was recorded for ENR-BSA antisera at 1/100th antiserum dilution in third IC.

Conclusion: AMP and ENR antibiotics proved to be good immunogens when conjugated to BSA by carbodiimide reaction with EDC as crosslinker. The polyclonal antibodies produced can be employed for detecting AMP and ENR residues in milk and urine samples.

Keywords: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, antibodies against antibiotics, conjugation, indirect competitive enzyme linked immunosorbent assay.

Maggot debridement therapy as primary tool to treat chronic wound of animals

Review (Published online: 25-04-2016)

12. Maggot debridement therapy as primary tool to treat chronic wound of animals - Vijayata Choudhary, Mukesh Choudhary, Sunanda Pandey, Vandip D. Chauhan and J. J. Hasnani

Veterinary World, 9(4): 403-409

   doi: 10.14202/vetworld.2016.403-409

Abstract

---

Maggot debridement therapy (MDT) is a safe, effective, and controlled method of healing of chronic wounds by debridement and disinfection. In this therapy live, sterile maggots of green bottle fly, Lucilia (Phaenicia) sericata are used, as they prefer necrotic tissues over healthy for feeding. Since centuries, MDT is used in human beings to treat chronic wounds. Lately, MDT came out as a potent medical aid in animals. In animals, although, this therapy is still limited and clinical studies are few. However, with the increasing antibiotic resistance and chronic wound infections in veterinary medicine, maggot therapy may even become the first line of treatment for some infections. This paper will present a brief discussion of MDT and its role in veterinary medicine that may add one more treatment method to utilize in non-healing wounds of animals and overcome the use of amputation and euthanasia. The objective of this review paper is to assemble relevant literature on maggot therapy to form a theoretical foundation from which further steps toward clinical use of maggot therapy in animals for chronic wounds can be taken.

Keywords: chronic wounds, debridement, Lucilia sericata, maggots, maggot debridement therapy.

Effect of supplemental heat on mortality rate, growth performance, and blood biochemical profiles of Ghungroo piglets in Indian sub-tropical climate

Research (Published online: 22-04-2016)

11. Effect of supplemental heat on mortality rate, growth performance, and blood biochemical profiles of Ghungroo piglets in Indian sub-tropical climate - Hemanta Nath, Mousumi Hazorika, Dipjyoti Rajkhowa, Mrinmoy Datta and Avijit Haldar

Veterinary World, 9(4): 396-402

   doi: 10.14202/vetworld.2016.396-402

Abstract

---

Aim: The present study was conducted to explore the effect of supplemental heat on mortality rate, growth performance, and blood biochemical profiles of indigenous Ghungroo piglets in sub-tropical cold and humid climatic conditions of Tripura, a state of the north eastern hill (NEH) region of India.

Materials and Methods: The experiment was conducted on 38 indigenous Ghungroo piglets from birth up to 60 days of age. Among the 38 piglets, 19 piglets were provided with supplemental heat ranging between 17.0°C and 21.1°C for the period of the first 30 days and thereafter between 24.1°C and 29.9°C for the next 30 days. The other 19 piglets were exposed to natural environmental minimum temperatures ranging between 7.2°C and 15.0°C during the first 30 days and then between 18.5°C and 25.5°C for the next 30 days.

Results: The supplemental heat resulted in 10.6% reduction of piglet mortality from the 2nd till the 7th day of age. These beneficial effects could be related with the lower (p<0.05) plasma glutamate pyruvate transaminase (GPT) and cortisol levels and higher (p<0.05) plasma alkaline phosphatase (AP) concentrations in heat supplemented group compared to control group. Plasma AP, GPT, glucose, triiodothyronine, and luteinizing hormone concentrations decreased (p<0.05) gradually with the advancement of age in both control and supplemental heat treated piglets.

Conclusion: Supplemental heat could be beneficial since it is related to a reduction of piglet mortality during the first week of life under farm management system in the sub-tropical climate of NEH region of India.

Keywords: biochemical profiles, Ghungroo piglets, growth, mortality rate, neonatal, supplemental heat.

Advances in reproductive biotechnologies

Review (Published online: 18-04-2016)

10. Advances in reproductive biotechnologies - K. K. Choudhary, K. M. Kavya, A. Jerome, and R. K. Sharma

Veterinary World, 9(4): 388-395

   doi: 10.14202/vetworld.2016.388-395

Abstract

---

In recent times, reproductive biotechnologies have emerged and started to replace the conventional techniques. It is noteworthy that for sustained livestock productivity, it is imperative to start using these techniques for facing the increasing challenges for productivity, reproduction and health with impending environment conditions. These recent biotechniques, both in male and female, have revolutionized and opened avenues for studying and manipulating the reproductive process both in vitro and in vivo in various livestock species for improving tis efficiency. This review attempts to highlight pros and cons, on the recent developments in reproductive biotechnologies, both in male and female in livestock species.

Keywords: assisted reproductive technologies, biotechnology, livestock, reproduction.

Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques

Research (Published online: 15-04-2016)

9. Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques - Gursimran Filia, Geeta Devi Leishangthem, Vishal Mahajan and Amarjit Singh

Veterinary World, 9(4): 383-387

   doi: 10.14202/vetworld.2016.383-387

Abstract

---

Aim: The aim of this study was to investigate the prevalence of bovine tuberculosis (TB) and detection of Mycobacterium bovis in cattle from an organized dairy farm.

Materials and Methods: A total of 121 animals (93 females and 28 males) of 1 year and above were studied for the prevalence of bovine TB using single intradermal comparative cervical tuberculin (SICCT) test, bovine gamma-interferon (γ-IFN) enzyme immunoassay, and polymerase chain reactions (PCRs).

Results: Out of total 121 animals, 17 (14.04%) animals were positive reactors to SICCT test while only one (0.82%) animal for γ-IFN assay. By PCR, Mycobacterium TB complex was detected in 19 (15.70%) animals out of which 4 (3.30%) animal were also positive for M. bovis.

Conclusions: Diagnosis of bovine TB can be done in early stage in live animals with multiple approaches like skin test followed by a molecular technique like PCR which showed promising results.

Keywords: bovine gamma interferon assay, bovine tuberculosis, Mycobacterium bovis, Mycobacterium tuberculosis complex, polymerase chain reaction, single intradermal comparative cervical tuberculin test.