Detection of extended-spectrum β-lactamases (blaCTX-M-1 and blaTEM) in Escherichia coli, Salmonella spp., and Klebsiella pneumoniae isolated from poultry in North Eastern India

Research (Published online: 30-11-2014)

23. Detection of extended-spectrum β-lactamases (blaCTX-M-1 and blaTEM) in Escherichia coli, Salmonella spp., and Klebsiella pneumoniae isolated from poultry in North Eastern India - H. Lalzampuia, T. K. Dutta, Iadarilin Warjri and Rajesh Chandra

Veterinary World, 7(11): 1026-1031

   doi: 10.14202/vetworld.2014.1026-1031

---

Abstract

---

Aim: The present study was conducted to record the association of extended spectrum β-lactamases (ESBLs) producing enteric bacteria with diarrhea of poultry birds in Mizoram, India.

Materials and Methods: Fecal samples were collected from poultry birds with the history of diarrhea from different parts of Mizoram. Samples were processed for isolation and identification of Escherichia coli, Salmonella, and Klebsiella pneumoniae. All the isolates were subjected to antibiotic sensitivity assays. Phenotypically, ESBLs production ability was determined by double discs synergy test (DDST) method. ESBLs producing isolates were subjected to polymerase chain reaction (PCR) for detection of ESBLs genes. Plasmids were cured by acridine orange. Transfer of resistance from donor to recipient strains was done by in vitro horizontal method.

Results: A total of 134 enteric bacteria was isolated, of which 102 (76.12%), 21 (15.67%) and 11 (8.21%) were E. coli, Salmonella spp. and K. pneumoniae, respectively. By DDST 7 (5.22%) isolates (6 E. coli and 1 K. pneumoniae) were ESBLs producer. PCR analysis confirmed 5 (3.73%) (4 E. coli and 1 K. pneumoniae) isolates harboured blaCTX-M-1 gene and/or blaTEM gene. All the isolates were carrying plasmids ranging between 0.9 kb and ~30 kb. Of the 4 isolates positive for blaCTX-M-1 and/or blaTEM, 2 (1.84%) were confirmed for blaCTX-M-1 gene in their plasmid. No blaTEM gene was detected from plasmid. The resistance plasmid could not be transferred to the recipient by in vitro horizontal gene transfer method.

Conclusion: ESBLs producing enteric bacteria are circulating in poultry in North Eastern Region of India. As poultry is one of the most common food animals in this region, these organisms may enter in human population through them.

Keywords: blaCTX-M-1, blaTEM, extended spectrum β-Lactamases, North East India, poultry.

Bone marrow derived cell-seeded extracellular matrix: A novel biomaterial in the field of wound management

Research (Published online: 30-11-2014)

22. Bone marrow derived cell-seeded extracellular matrix: A novel biomaterial in the field of wound management - V. Remya, Naveen Kumar, A. K. Sharma, Dayamon D. Mathew, Mamta Negi, S. K. Maiti, Sameer Shrivastava, S. Sonal and N. P. Kurade

Veterinary World, 7(11): 1019-1025

   doi: 10.14202/vetworld.2014.1019-1025

---

Abstract

---

Aim: Extensive or irreversible damage to the skin often requires additional skin substitutes for reconstruction. Biomaterials have become critical components in the development of effective new medical therapies for wound care.

Materials and Methods: In the present study, a cell matrix construct (bone marrow-derived cells (BMdc) seeded extracellular matrix [ECM]) was used as a biological substitute for the repair of full-thickness skin wound. ECM was developed by decellularizing fish swim bladder (FSB). Goat bone marrow-derived cells (G-BMdc) were seeded over this decellularized matrix. Efficacy of this cell matrix construct in wound repair was tested by implanting it over 20 mm2 × 20 mm2 size fullthickness skin wound created over the dorsum of rat. The study was conducted in 16 clinically healthy adult rats of either sex. The animals were randomly divided into 2 equal groups of 8 animals each. In Group I, animal’s wounds were repaired with a cellular FSB matrix. In Group II, wounds were repaired with G-BMdc seeded a cellular FSB matrix. Immune response and efficacy of healing were analyzed.

Results: Quality of healing and immuno tolerance to the biological substitute was significantly better in Group II than Group I.

Conclusion: Seeding with BMdc increases the wound healing potency and modulates the immune response to a significantly negligible level. The BMdc seeded acellular FSB matrix was found to be a novel biomaterial for wound management.

Keywords: biomaterial, decellular, extra cellular matrix, wound.

The effectiveness of novel bacteriocin derived from Escherichia coli colonized in the fermented pineapple Ananas comosus (L.) Merr. against pathogenic bacteria isolated from aquaculture sites

Research (Published online: 30-11-2014)

21. The effectiveness of novel bacteriocin derived from Escherichia coli colonized in the fermented pineapple Ananas comosus (L.) Merr. against pathogenic bacteria isolated from aquaculture sites - S. W. Lee, W. Wendy, L. Montira and A. U. Siti Hazirah

Veterinary World, 7(11): 1014-1018

   doi: 10.14202/vetworld.2014.1014-1018

---

Abstract

---

Aim: The aim was to evaluate antimicrobial property of bacteriocin isolated from Escherichia coli against pathogenic bacteria from aquaculture sites.

Materials and Methods: E. coli was isolated from fermented pineapple Ananas comosus using eosin methylene blue agar. The antimicrobial activity of the isolated E. coli was screened using hole-plate diffusion method. The bacterial strain that showed the widest inhibition zone was selected and grown in tryptic soy broth, followed by partial purification of bacteriocin by using ammonium sulphate. Bacteriocin derived from the E. coli was subjected to the antimicrobial test against 55 bacteria strains namely Aeromonas hydrophila(n=10), Citrobacter freundii (n=5), Edwardsiella tarda (n=10), Flavobacterium spp. (n=10), Pseudomonas spp. (n=10), Vibrio parahaemolyticus (n=5) and Vibrio alginolyticus (n=5) by using twofold broth microdilution method to determine minimum inhibitory concentration (MIC) values of the bacteriocin against the tested bacteria.

Results: The results of the present study showed that the MIC values of the partially purified bacteriocin against present pathogenic bacteria isolates ranged from 7.81 to 31.25 ppm whereas the MIC values of kanamycin (positive control) ranged from 15.63 to 125 ppm.

Conclusion: The results of the present study showed the bacteriocin derived from E. coli can control all the present bacterial isolates indicating the huge potential of the bacteriocin as a new antimicrobial agent for aquaculture uses.

Keywords: Escherichia coli, partially purified bacteriocin, pathogenic bacteria.

Size tunable gold nanoparticle and its characterization for labeling application in animal health

Research (Published online: 27-11-2014)

20. Size tunable gold nanoparticle and its characterization for labeling application in animal health - P. R. Sahoo and Praveen Singh

Veterinary World, 7(11): 1010-1013

   doi: 10.14202/vetworld.2014.1010-1013

---

Abstract

---

Aim: The aim of the present study was to synthesize different sizes of gold nanoparticles (GNPs) and their characterization for use as a label in lateral flow assay particular for the detection of bluetongue in small ruminants.

Materials and Methods: Size controlled synthesis of GNPs was done by using different concentration of sodium citrate. In this study, five different types of GNP were synthesized by using trisodium citrate (Na3C6H5O72H2O) that reduces 20 mM concentration of gold solution (HAuCl4). These different types of GNPs were characterized in terms of morphology, size, shape and λmax by transmission electron microscopy and ultraviolet-visible spectroscopy respectively.

Results: In the present work, it was found that the size of GNP mainly depends upon the concentration of sodium citrate. By use of 0.09375%, 0.1875%, 0.375%, 0.75% and 1.5% of sodium citrate solution, GNPs were synthesized. In our study, the size of GNP was found ranging from 25 nm to 230 nm. The size was found large with less concentration of sodium citrate (i.e. with 0.09735%) and small with large concentration of sodium citrate (1.5%) and λmax was found to be 450-530 nm in all size of GNP.

Conclusions: The size of GNPs is mainly dependant on the concentration of trisodium citrate, gold salt concentration, optimum pH and temperature. The GNP synthesized by this method has been used as a label for the development of lateral flow assay against diagnosis of bluetongue disease in small ruminant.

Keywords: gold nanoparticles, size controlled synthesis, sodium citrate, tetrachloroauric acid, transmission electron microscopy.

Prevalence of Listeria monocytogenes in ready-to-eat seafood marketed in Thessaloniki (Northern Greece)

Research (Published online: 27-11-2014)

19. Prevalence of Listeria monocytogenes in ready-to-eat seafood marketed in Thessaloniki (Northern Greece) - N. Soultos, E. Iossifidou, Z. Tzikas, D. Sergelidis, Th. Lazou, G. Drakopoulos and I. Konstantelis

Veterinary World, 7(11): 1004-1009

   doi: 10.14202/vetworld.2014.1004-1009

---

Abstract

---

Aim: In the current study, a contribution to the knowledge on the prevalence and level of contamination of Listeria monocytogenes in ready-to-eat (RTE) seafood marketed in Thessaloniki (Northern Greece) was provided; the serovar identity of the L. monocytogenesisolates was also determined.

Materials and Methods: A total of 132 RTE seafood samples consisting of 74 smoked fish products, 18 salted fish products, 16 dried fish products, 9 raw marinated fish products, 10 cooked marinated cephalopods and 5 surimi crab stick products were analyzed. L. monocytogenes were isolated and enumerated based on ISO 11290-1/A1 and ISO 11290-2/A1 protocols, respectively, and identified using a multiplex polymerase chain reaction (PCR) system utilizing genus and species specific primers. For the identification of serotypes a second multiplex PCR assay was used which clusters L. monocytogenes strains into four major serogroups.

Results: Of the samples examined, 11 (8.3%) proved positive for Listeria spp. with 8 (6.1%) yielding L. monocytogenes. Only in one sample of smoked mackerel the level of L. monocytogenes exceeded the legal safety limit of 100 cfu/g set out in Commission Regulation (EC) No. 1441/2007. Serotyping showed higher percentages of isolates belonging to PCR serogroup 3:1/2b, 3b, 7 (46.7%) and serogroup 1:1/2a, 3a (40%) followed by serogroup 4:4b, 4d, 4e (13.3%).

Conclusion: This study demonstrated that L. monocytogenes can be isolated from processed RTE seafood products at retail in Thessaloniki (Northern Greece) in low concentrations. However, the presence of this human pathogen in RTE seafood should not be overlooked, but it should be considered as having significance public health implications, particularly among the persons who are at greater risk. Therefore, RTE seafood should be produced under appropriate hygienic and technological conditions since the product does not undergo any treatment before consumption.

Keywords: level, Listeria monocytogenes, Northern Greece, prevalence, ready-to-eat seafood, serotyping.

Seroepidemiological pattern of leptospirosis in bovine of South Gujarat, India

Research (Published online: 27-11-2014)

18. Seroepidemiological pattern of leptospirosis in bovine of South Gujarat, India - J. M. Patel, P. D.Vihol, M. C. Prasad, I. H. Kalyani, J. K. Raval, K. M. Patel, Sushma R. A. Thirumalesh and V. Balamurugan

Veterinary World, 7(11): 999-1003

   doi: 10.14202/vetworld.2014.999-1003

---

Abstract

---

Aim: Seroepidemiological study of leptospirosis in cattle of various South Gujarat district (Navsari, Tapi, Surat, Valsad).

Materials and Methods: Whole blood samples were collected randomly from different age groups, and breeds of cattle of either sex reared in different districts (Navsari, Surat, Tapi, Valsad) of South Gujarat. To obtain serum, whole blood was kept in slanting position in 9.0 ml plain vacutainers until serum extracted out of the whole blood. Then these 9.0 ml plain vacutainers were centrifuged at 7000 rpm for 10 min. The straw colored serum was then collected in 1.5 ml sterile cryo vials and aliquoted and stored at −20°C for microscopic agglutination test.

Results: In the present study, overall 12.81% (51/398) seroprevalence were recorded with highest seroprevalence (47.06%, 24/51) from Valsad followed by Navsari (9.14%, 18/197), Surat (6.90%, 2/29) and Tapi (5.79%, 7/121) among cattle. The seroprevalence rate of breed and sex wise did not differ significantly (p≤0.05). Maximum incidence of seropositivity was found above 4 years (16.32%, 39/239) of age group followed by animals between 1 and 4 years (9.68%, 12/124). Thus, the age was significantly influencing the seropositivity (p≤0.05). In cattle out of 398 sera screened, 51 were positive with one or more serovars. The highest seropositivity was recorded against serovar Pomona (28.89%).

Conclusions: Overall 12.81% seroprevalence of leptospirosis in apparently healthy and clinically ailing bovine of South Gujarat indicating potential zoonotic risk to farmers, labor, and animal owners.

Keywords: cattle, leptospirosis, seroepidemiology, zoonosis.

Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis

Research (Published online: 27-11-2014)

17. Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis - Gamil S. G. Zeedan, Abeer M. Abdalhamed, Eman Abdeen, Mahmoud E. Ottai and Sobhy Abdel-Shafy

Veterinary World, 7(11): 991-998

   doi: 10.14202/vetworld.2014.991-998

---

Abstract

---

Aim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and public-health point of view. The present study aimed to isolate and identify of bacteria causes mastitis in dairy cows and to evaluate the antibacterial activities of some selected medicinal plants extracts comparing antibiotics used in the treatment of mastitis in Egypt.

Materials and Methods: A total of 203 milk samples of dairy cows were collected during the period from February to June 2013 at different Governorates in Egypt. The use clinical inspection and California mastitis test examination were provided efficient diagnostic tool for detection of clinical, subclinical mastitis and apparently normal health cattle. The collected milk samples were cultured on Nutrient, Blood agar, Mannitol salt, Edward’s and MacConkey agar plates supporting the growth of various types of bacteria for their biochemical studies and isolation. The antimicrobial activity of plants extracts (Jasonia montana and Artemisia herb alba)with different solvent (ethanol, petroleum ether, chloroform and acetone)were studied in vitro against isolated bacteria from mastitis by paper desk diffusion and minimum inhibitory concentration method (MIC).

Results: The prevalence of clinical, subclinical mastitis and normal healthy animals were 34.50%, 24.7% and 40.8% respectively. The major pathogens isolated from collected milk samples were Escherichia coli (22.16%), Staphylococcus aureus (20.19%), Streptococcusspp. (13.3%), Streptococcus agalactiae (12.8%), Streptococcus dysgalactia (0.5%), Pasteurella spp. (2.45%), Klebsiella spp. (1.47%)andPseudomonas spp. (0.45%). The highest antibacterial activity of J. montana plant extracted with acetone solvent against S. agalactiae, E. coli, S. aureus, Klebsiella spp and coagulase-negative Staphylococci with zone of inhibition values ± standard deviation (SD), ranging from 4.33±0.57 to 25.6±0.60 mm. The MIC values for the extracts ranged from 0.01 to 1.56 mg/ml. when comparing antibacterial activity of A. herb alba plant extracted with acetone solvent on the same bacteria with zone of inhibition values ± SD, ranging from 00±00 to 5.6±0.60 mm. Both extracts from J. montana and A. herb alba plant extracts with petroleum ether, methanol and chloroform solvent were less antibacterial activities than acetone solvent extract.

Conclusion: The present study spot highlight on isolation and identification of mastitis pathogens that are fundamental aspects of milk quality, udder health control programs and public health and food safety issues associated with food borne pathogens. J. montana and A. herb alba plants have antibacterial effects more than antibiotics used in the treatment of mastitis. Finally, the medicinal plant extracts can be used to discover bioactive natural product in the form of antibacterial that may be serve the development of new pharmaceutical products. But still need further research necessary to identify active compounds and research to mechanism and drug interaction.

Keywords: antimicrobial agent, Artemisia herb alba, California mastitis test, Jasonia montana, minimum inhibitory concentration.