Eucalyptus viminalis leaf extract alters the productivity and blood parameters of healthy broiler chickens

Research (Published online: 16-12-2020)

13. Eucalyptus viminalis leaf extract alters the productivity and blood parameters of healthy broiler chickens

G. K. Duskaev, O. V. Kvan and Sh. G. Rakhmatullin

Veterinary World, 13(12): 2673-2680

ABSTRACT

Background and Aim: As an alternative to natural and chemically synthesized direct-acting bactericides, there has been an increase in the use of plant extracts, which possess a set of phytochemicals with potential for microbial disease control; this is due to the spectrum of secondary metabolites present in extracts, which include phenolic compounds, quinones, flavonoids, alkaloids, terpenoids, and polyacetylenes. The biologically active substances within plant extracts, which perform protective functions for plant tissues, can have ambiguous effects on the animal body. Therefore, the aim of this study was to assess the ability of gamma-octalactone, isolated from Eucalyptus viminalis extract, to inhibit various LuxI/LuxR quorum-sensing (QS) systems in bacteria, and to evaluate its effect on broiler chickens.

Materials and Methods: Phytochemical analysis of E. viminalis extract was performed. The ability of gamma-octalactone to inhibit QS was evaluated using four different LuxI/LuxR bacterial test systems. In vivo assessments were performed on one hundred and twenty 7-day-old broiler chickens (Arbor Acres cross), split into four groups of 30 chickens: 1. Control group: Basic diet (BD); 2. experimental Group I: BD + gamma-octalactone at a dosage of 0.05 ml/kg live weight/day; 3. experimental Group II: BD + gamma-octalactone at a dosage of 0.1 ml/kg live weight/day; and 4. experimental Group III: BD + gamma-octalactone at a dosage of 0.2 ml/kg live weight/day. Hematological blood parameters were assessed using an automatic hematological analyzer (URIT-2900 Vet Plus, URIT Medial Electronic Co., China) and an automatic biochemical analyzer (CS-T240, Dirui Industrial Co., Ltd., China). Statistical analyses were performed using SPSS Statistics Version 20 (IBM); averages (M), standard deviations (σ), and standard deviation errors (m) were calculated. Results with p≤0.05 were considered significant.

Results: Based on the phytochemical analysis results, libraries of compounds with putative QS inhibitory properties were compiled. Gamma-octalactone exhibited a pronounced inhibitory effect on the LuxI/LuxR QS systems, characterized by EC50 values of 0.15-0.4 mM. In the in vivo portion of this study, broiler chicken live weights increased in all experimental groups, with the most significant increase in Group III (14.0%), in relation to the control group. Blood serum from the experimental group chickens had significantly higher levels of triglycerides and uric acid (p≤0.05), in comparison to the control group chickens. With respect to blood serum enzyme activity and antioxidant status indicators, the experimental group chickens had a higher level of gamma-glutamyl transferase, an enzyme associated with amino acid metabolism, than those in the control group; this increase was especially pronounced in Group III, with 37.0% increase (p≤0.05). Superoxide dismutase and catalase levels were higher in the experimental groups than the control group, corresponding to increases of 30.4-56.2% (p≤0.05), 33.3-83.3%, and 27.9-45.5% (p≤0.05) in Groups I, II, and III (p≤0.05), respectively. Morphological blood parameters did not display significant changes due to gamma-octalactone.

Conclusion: According to the results of this in vivo study in broiler chickens, gamma-octalactone, isolated from E. viminalis leaf extract and supplied at a dosage of 0.2 ml/kg live weight/day, led to an increase in the activity of blood plasma digestive enzymes, increased live weight, and had a positive effect on lipid metabolism and antioxidant status.

Keywords: broiler chickens, Eucalyptus viminalis leaf extract, gamma-octalactone, productivity, quorum sensing.

Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

Research (Published online: 16-12-2020)

12. Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

Nalisa Khochamit, Surasak Siripornadulsil, Peerapol Sukon and Wilailak Siripornadulsil

Veterinary World, 13(12): 2663-2672

ABSTRACT

Aim: The aim of the study was to determine the potentials and effects of Bacillus subtilis and lactic acid bacteria (LAB) as probiotics on broiler growth, health, and Salmonella infection.

Materials and Methods: To evaluate the inoculum size applicable for broilers, 1-day-old broilers were orally fed fresh cultures of single strains and a B. subtilis KKU213/Pediococcus pentosaceus NP6 mixture at 108 and 1012 colony-forming unit (CFUs)/mL/chick. The body weight gain (BWG), Salmonella contamination level and total Bacillus and LAB abundances in the crop and intestine were measured. Subsequently, 1-day-old broilers were orally fed of KKU213, CH403, and Pediococcus acidilactici SH8 at 1010 CFUs/mL, followed by inulin. After 35 days, the BWG, Bacillus and LAB abundances in the cecum, blood parameters, and KKU213 colonization were assessed.

Results: The broilers fed single strains or KKU213+NP6 exhibited a higher BWG and a higher crop LAB abundance than the controls (p<0.05). Probiotic feeding decreased the intestinal Salmonella abundance and correspondingly increased the LAB abundance. The broilers fed the mixed culture (KKU213+CH403+SH8) followed by prebiotics showed lower mortality, higher blood high-density lipoprotein levels, and lower blood uric acid levels than the controls (p<0.0004). Probiotic feeding significantly increased the Bacillus and LAB counts (p<0.05). A CE330 isolate obtained from the cecum after 35 days of KKU213 feeding was closely related to B. subtilis KKU213.

Conclusion: B. subtilis KKU213 is a potent probiotic strain that can survive, colonize and reduce Salmonella infection in broilers and improve their growth and health. This strain, combined with different LAB can act synergistically in the gut and promote broiler growth.

Keywords: broiler, Bacillus subtilis, lactic acid bacteria, probiotics.

Royal Jelly and Aliskiren mutually annul their protective effects against gentamicin-induced nephrotoxicity in rats

Research (Published online: 14-12-2020)

11. Royal Jelly and Aliskiren mutually annul their protective effects against gentamicin-induced nephrotoxicity in rats

Mohd Alaraj

Veterinary World, 13(12): 2658-2662

ABSTRACT

Background and Aim: Gentamicin (GM) is one of the most effective antibiotics for severe, life-threatening Gram-negative infections. Nevertheless, its clinical use has been restrained because of its nephrotoxic potential. Royal jelly (RJ) and aliskiren (ALK) can individually prevent such toxic effects. The aim of this study was to explore the protective effects of a combination treatment of RJ and ALK on GM-mediated nephrotoxicity.

Materials and Methods: Thirty-two adult female Wistar rats were divided equally into four groups: (I) Receiving normal saline; (II) GM (100 mg/kg, intraperitoneal [i.p.] injection); (III) GM (100 mg/kg, i.p. injection) plus ALK (50 mg/kg, i.p. injection); and (IV) GM (100 mg/kg, i.p. injection) plus ALK (50 mg/kg, i.p. injection) in combination with RJ (150 mg/ kg, orally). All treatments were administered daily for 10 days. The blood levels of creatinine, urea, uric acid, albumin, and total protein were measured. Then, the animals were sacrificed, and the kidneys were taken for histopathology.

Results: Compared to normal control rats, GM-injected rats showed significantly (p<0.001) higher serum concentrations of uric acid, urea, and creatinine as well as evidently (p<0.001) lower blood levels of albumin and total protein. Moreover, GM administration was associated with significant renal histopathological changes. All these alterations were considerably (p<0.05) improved in GM-injected rats receiving ALK compared to rats receiving GM alone. However, when RJ was given in combination with ALK to GM-injected rats, it lessened the beneficial nephroprotective effects of both agents.

Conclusion: The combination treatment of RJ and ALK is not desirable for GM-induced nephrotoxicity. Further studies are crucial to accurately explore the precise mechanism of RJ antagonistic interaction with ALK.

Keywords: aliskiren, gentamicin, nephrotoxicity, royal jelly.

Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

Research (Published online: 14-12-2020)

10. Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

Berlin Pandapotan Pardede, Muhammad Agil, Yudi Yudi and Iman Supriatna

Veterinary World, 13(12): 2649-2657

ABSTRACT

Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility.

Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen-thawed semen samples from eight bulls with the same production code were analyzed and compared between the production site (artificial insemination [AI] center) and the field (BX breeding program). Total and progressive motility (PM) of sperm were determined using computer-assisted semen analysis. Plasma membrane integrity (PMI) was assessed using hypoosmotic swelling test, sperm viability using Eosin-Nigrosin staining, acrosome integrity using trypan blue-Giemsa staining, morphological abnormalities using William staining, and DNA fragmentation using toluidine blue staining. The fertility rate was determined using the conception rate (%) derived from AI data based on 502 AI services and 478 cows in the BX breeding program. A t-test was used to compare the quality of frozen-thawed semen before and after distribution. The relationship between the qualities of frozen semen after distribution in the field with fertility was analyzed using Pearson correlation.

Results: There was no significant difference (p>0.05) in the quality of frozen-thawed semen (sperm motility, PMI, viability, acrosome integrity, abnormalities, and DNA fragmentation) between the production site (AI center) and after distribution in the field (BX breeding program). The semen met the minimum standards for AI programs. Total motility (r=0.986), PM (r=0.961), sperm viability (r=0.971), PMI (r=0.986), and acrosome integrity (r=0.992) were all positively correlated (p<0.05) with fertility rate; while sperm abnormalities (r=-0.996) and sperm DNA fragmentation (r=0.975) were negatively correlated (p<0.05) with fertility rate.

Conclusion: The study showed that to achieve the maximal and optimal fertility rate in bulls in an AI program, the overall quality of frozen-thawed semen in all aspects is critical. This can be achieved if the handling during distribution and storage, as well as the various factors that may affect the quality of semen in the field, can be controlled properly.

Keywords: artificial insemination, Brahman cross, distribution, fertility rate, frozen semen quality.

Parasitic helminth infections of dogs, wolves, foxes, and golden jackals in Mazandaran Province, North of Iran

Research (Published online: 12-12-2020)

9. Parasitic helminth infections of dogs, wolves, foxes, and golden jackals in Mazandaran Province, North of Iran

Abolghasem Siyadatpanah, Abdol Sattar Pagheh, Ahmad Daryani, Shahabeddin Sarvi, Seyed Abdollah Hosseini, Roghayeh Norouzi, Larson Boundenga, Fatemeh Tabatabaie, Maria de Lourdes Pereira, Shirzad Gholami and Veeranoot Nissapatorn

Veterinary World, 13(12): 2643-2648

ABSTRACT

Background and Aim: There is a large amount of information on intestinal parasites in stray dogs and golden jackals (Canis aureus) in Mazandaran Province, Iran. However, there is little information about foxes and wolves, which have a potential role in the spread of dangerous parasitic diseases, such as echinococcosis and toxocariasis. The aim of the present study was to identify the genus or species of parasitic worms in stool samples obtained from carnivores in Mazandaran Province, Iran, from August 2017 to April 2018.

Materials and Methods: A total of 274 fecal samples were collected from carnivores, including dog, fox, wolf, and C. aureus in three areas of Mazandaran Province, Iran. All specimens were examined by centrifugal fecal flotation using a solution of Sheather's sugar to detect helminths eggs. Then, all samples were assessed using a light microscope. Data analysis was performed by SPSS version. 18 (Chicago, IL, USA).

Results: In this study, seven genera of helminths were observed, including Ancylostoma, Uncinaria, Toxocara, Dipylidium, Toxascaris, Taenia, and Spirocerca. The prevalence of helminth infections was 97.7% (127 out of 130), 56.7% (51 out of 90), 51.4% (18 out of 35), and 52.6% (10 out of 19), among dogs, C. aureus, foxes, and wolves, respectively. The highest prevalence of Ancylostoma and Toxocara infections occurred in the eastern and central areas of the province (42.1% and 35.7%, respectively).

Conclusion: Based on the results of this study, the infection with intestinal zoonotic helminths in carnivores was an important public health factor in Mazandaran. Therefore, these infections can be potentially harmful to humans and other animals.

Keywords: carnivores, environmental contamination, helminth, intestinal parasites, Iran.

Assessment of biphasic calcium phosphate 70/30 alginate scaffold on the tibia in pigs

Research (Published online: 11-12-2020)

8. Assessment of biphasic calcium phosphate 70/30 alginate scaffold on the tibia in pigs

Gunanti Soeyono, Kiagus Dahlan, Melpa Susanti Purba, Sus Dherthi Widhyari, Rr. Soesatyoratih, Thang Shi Teng, Lieonny Budiarti, Ho Kin Wai and Agatha Kosat

Veterinary World, 13(12): 2635-2642

ABSTRACT

Background and Aim: Calcium phosphate bioceramics have been used for at least a decade, and many investigations have focused on the use of hydroxyapatite (HA) derivative in the regeneration of bone defects. Biphasic calcium phosphate (BCP) is a biomaterial composed of HA and beta-tricalcium phosphate (BCP), with a structure similar to bone. The aim of the study was to determine the influence of the BCP/alginate scaffold on tissue growth, blood, the lungs, and the electrical activity of the heart during bone healing in the tibia of pig.

Materials and Methods: Three pigs were implanted with BCP/alginate scaffolds in the tibias. Pigs were acclimatized and treated with antibiotics and anthelminthic drugs 14 days before implantation. Each pig was implanted with a BCP/ alginate scaffold in the right tibia and a defect without the implant was made in the left tibia as the control. Radiographic images of the tibia were captured 0, 7, 30, and 60 days after the operation. Erythrograms, radiography of the lungs, and electrocardiogram (ECG) recordings were done 0, 30, and 60 days after the operation.

Results: Radiographic evaluations showed that the implant and peri-implant density of BCP decreased throughout the process of bone healing. The erythrogram profile indicated that a substantial amount of time (60 days) was required to adapt and return to pre-operative conditions. No significant differences in ECG recordings or pulmonary radiography were detected.

Conclusion: The BCP/alginate scaffold did not induce a faster recovery rate from the bone defect compared to the control with no implant. However, the BCP/alginate scaffold was biodegradable, bioresorbable, and non-toxic.

Keywords: biphasic calcium phosphate, bone implant, erythrogram, pig, pulmonary radiography, scaffold.

The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

Research (Published online: 11-12-2020)

7. The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

Alek Ibrahim, I Gede Suparta Budisatria, Rini Widayanti and Wayan Tunas Artama

Veterinary World, 13(12): 2625-2634

ABSTRACT

Background and Aim: Java Island is one of the islands in Indonesia which has local sheep breeds with specific characteristics and native development geography in certain regions. This study aimed to determine the genetic profiles and maternal origin of six local sheep breeds on Java Island.

Materials and Methods: This study was conducted by identifying the profiles of complete mitochondrial DNA (mtDNA) displacement loop (D-loop) region sequences on a total of 22 individual in six local sheep breeds on Java Island, including Javanese thin-tailed (JTT), Javanese Fat-Tailed (JFT), Batur (BTR), Wonosobo (WSB), Garut (GRT), and Priangan (PRG) sheep. The D-loop region was amplified using specific primers, and the polymerase chain reaction (PCR) was performed. The PCR products were purified and sequenced.

Results: The mtDNA D-loop analysis identified 21 haplotypes in the analyzed 22 animals with 123 polymorphic sites (V) consisting of 60 singleton variable sites (S) and 63 parsimony informative sites (P). Within all breeds tested, the haplotype diversity, the average number of pairwise differences (K), and nucleotide diversity (Pi) were 0.99567, 25.36364, and 0.02153, respectively. The genetic distance (D) within groups and between groups was 0.001-0.006 and 0.004-0.036, respectively. The phylogeny resulted in the presence of two haplogroups (Hap), which are 5 Hap A and 16 Hap B. All JTT, JFT, BTR, and WSB breeds were in the same cluster in Hap B, whereas GRT and PRG breeds were in clusters in both Hap A and Hap B.

Conclusion: The high genetic diversity in six local sheep breeds on Java Island suggests that they originated from different genetic sources. JTT sheep have closer genetic relationships to JFT, BTR, and WSB sheep, and they are close to European sheep, whereas GRT sheep have closer genetic relationships to PRG sheep. Both are closer to Asian sheep than to European sheep.

Keywords: control region, genetic variation, indigenous sheep, Indonesian sheep, non-coding region, phylogenetic.