Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization

Research (Published online: 04-10-2019)

3. Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization

Linda Haryadi, Eddy Suprayitno, Aulanni'am Aulanni'am and Anik Martinah Hariati

Veterinary World, 12(10): 1529-1534

ABSTRACT

Background and Aim: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers' preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the Anisakis species which potent to induce an allergic reaction. However, the study about A. typica involved in the dendritic cells (DCs), T helper 1 (Th1), T helper 2 (Th2), and regulatory T cells (Tregs) is still limited. This study aimed to analyze the dynamic changed of the immune system including DCs, CD4+ T cells, and Tregs after 1 week of A. typica sensitization.

Materials and Methods: Twenty-four male Balb/C mice were randomly divided into four groups (n=6), mice treated with crude A. typica extract (CAE) 50, 75, and 100 mg/kg BW, respectively. CAE was given orally per day for a week. At the end of the experiment, the animals were sacrificed and the spleen was collected. DCs were labeled as CD11c+ interleukin-6+ (IL-6+); CD4+ T cells were distinguished as Th1 (CD4+ interferon-γ+ [IFN-γ+]) and Th2 (CD4+ IL-4+ and CD4+ IL-5+); Tregs were labeled as CD4+CD25+CD62L+. The expression of each cell was determined by flow cytometry.

Results: Our result described that CAE elicits CD11c+ IL-6+, CD4+ IFN-γ+, CD4+ IL-4+, and CD4+ IL-5+ and reduces CD4+CD25+CD62L+ significantly (p<0.05) in dose-dependent manner in mice after A. typica infection.

Conclusion: The Th1/Th2 ratio after A. typica crude extract treatment exhibits a mixed pattern rather than the classical model allergy to food antigens. Our study is expected as a basic understanding of the changes in immune response after A. typica infection.

Keywords: allergy, dendritic cells, inflammation, nematode, regulatory T cells.

Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat

Research (Published online: 04-10-2019)

2. Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat

A. N. Dapgh, A. S. Hakim, H. A. Abouelhag, A. M. Abdou and E. A. Elgabry

Veterinary World, 12(10): 1524-1528

ABSTRACT

Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are able to live everywhere and suggested to be the causative agent of great scope of acute and chronic animal infections.

Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance.

Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR).

Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates.

Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs.

Keywords: drug resistance, goat, Pseudomonas aeruginosa, sheep, virulence.

Seroprevalence and factors associated with bovine and swine toxoplasmosis in Bobo-Dioulasso, Burkina Faso

Research (Published online: 02-10-2019)

1. Seroprevalence and factors associated with bovine and swine toxoplasmosis in Bobo-Dioulasso, Burkina Faso

Dieudonne Tialla, Laibane Dieudonne Dahourou and Oubri Bassa Gbati

Veterinary World, 12(10): 1519-1523

Background and Aim: Toxoplasmosis is a worldwide zoonosis with major public health importance. To know more about this condition in Burkina Faso, this study was implemented to determine the seroprevalence of Toxoplasma gondii infection among pigs and cattle in intra-urban and peri-urban area of Bobo-Dioulasso (Burkina Faso).

Materials and Methods: Serum samples were collected from 600 cattle and 600 pigs with 300 samples from each species in intra-urban and peri-urban area of Bobo-Dioulasso. Data about age, sex, and breed of each animal were also noted. Serum samples were analyzed by indirect enzyme-linked immunosorbent assay to look for immunoglobulin G and immunoglobulin M antibodies to T. gondii.

Results: This study revealed a herd prevalence of 92.5% and 75%, respectively for porcine and bovine toxoplasmosis. At the individual level, we found a prevalence of 29% and 49.2% for cattle and pigs, respectively. For each species, we noticed a significant association between age, sex, breed husbandry system, and the presence of anti-T. gondii antibodies. The prevalence was significantly higher in female, intra-urban system, exotic breed, and animal <2 years old (p<0.05).

Conclusion: The results provided evidence for the presence of T. gondii in pigs and cattle farms around Bobo-Dioulasso. Hence, in Bobo-Dioulasso, raw or undercooked meat consumption is a risk for T. gondii infection for human. Knowledge of the prevalence of toxoplasmosis will help to target prevention efforts.

Keywords: Burkina Faso, cattle, public health, seroepidemiologic studies, swine, toxoplasmosis.

Hematological and biochemical reference values of Asian house shrews (Suncus murinus) in Bangladesh

Research (Published online: 30-09-2019)

24. Hematological and biochemical reference values of Asian house shrews (Suncus murinus) in Bangladesh

Md. Kaisar Rahman, Shariful Islam, Mizanur Rahman, Jinnat Ferdous, Sazeda Akter, Md. Mustafizur Rahaman, Mohammad Alamgir Hossain, Mohammad Mahmudu Hassan and Ariful Islam

Veterinary World, 12(9): 1514-1518

ABSTRACT

Background and Aim: Determining reference values for hematological and biochemical parameters of Asian house shrew (Suncus murinus) is important for wildlife research to protect human health in surrounding communities. This study aimed to establish the reference values for selected hematology and serum clinical chemistry analyses that may contribute to research on shrew in future.

Materials and Methods: Blood samples (n=51) were collected from shrews between July and December 2015, Bangladesh, to estimate the levels of hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), total erythrocyte count (TEC), lymphocyte, monocyte, neutrophil, eosinophil, basophil, calcium, phosphorus (P), sodium (Na), chloride (Cl), urea, glucose, total protein (TP), creatinine, and alanine transaminase (ALT).

Results: Although the values did not differ significantly among sexes, age was found to be a significant factor. Hb, PCV, TEC, glucose, and P were higher in males; eosinophil, Na, Cl, TP, and ALT were higher among females. Adults had significantly greater urea and glucose (p<0.05) while juveniles had insignificantly higher values for TLC, PCV, neutrophil, P, and TP.

Conclusion: This study provides the first reference values for this species in Bangladesh and can be used to guide wildlife research studies.

Keywords: Asian house shrew, hematology, reference interval, serum chemistry, Suncus murinus.

Molecular techniques for sex identification of captive birds

Research (Published online: 30-09-2019)

23. Molecular techniques for sex identification of captive birds

Medania Purwaningrum, Herjuno Ari Nugroho, Machmud Asvan, Karyanti Karyanti, Bertha Alviyanto, Randy Kusuma and Aris Haryanto

Veterinary World, 12(9): 1506-1513

ABSTRACT

Background and Aim: Many avian species are considered sexually monomorphic. In monomorphic bird species, especially in young birds, sex is difficult to identify based on an analysis of their external morphology. Accurate sex identification is essential for avian captive breeding and evolutionary studies. Methods with varying degrees of invasiveness such as vent sexing, laparoscopic surgery, steroid sexing, and chromosome inspection (karyotyping) are used for sex identification in monomorphic birds. This study aimed to assess the utility of a non-invasive molecular marker for gender identification in a variety of captive monomorphic birds, as a strategy for conservation.

Materials and Methods: DNA was isolated from feather samples from 52 individuals representing 16 species of 11 families indigenous to both Indonesia and elsewhere. We amplified the chromodomain helicase DNA-binding (CHD) gene using polymerase chain reaction with MP, NP, and PF primers to amplify introns with lengths that differ between the CHD-W and the CHD-Z genes, allowing sex discrimination because the W chromosome is exclusively present in females.

Results: Molecular bird sexing confirmed 33 females and 19 males with 100% accuracy. We used sequencing followed by alignment on one protected bird species (Probosciger aterrimus).

Conclusion: Sex identification may be accomplished noninvasively in birds, because males only have Z sex chromosomes, whereas females have both Z and W chromosomes. Consequently, the presence of a W-unique DNA sequence identifies an individual as female. Sexing of birds is vital for scientific research, and to increase the success rate of conservation breeding programs.

Keywords: bird, chromodomain helicase DNA-binding gene, molecular bird sexing, polymerase chain reaction, sexing.

Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure

Research (Published online: 29-09-2019)

22. Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure

Natthasit Tansakul, Jatuporn Rattanasrisomporn and Sittiruk Roytrakul

Veterinary World, 12(9): 1499-1505

ABSTRACT

Background and Aim: Unlike the already well-documented human serum proteome, there are still limitations regarding analyzing and interpreting the various physiological changes and disease states of the serum proteomes found in duck. Serum proteome in duck under the condition of mycotoxin contamination in feed has not yet been examined. This study aimed to introduce the characterization of the circulating proteomes in duck serum during exposure to aflatoxin B1 (AFB1).

Materials and Methods: Duck serum samples were collected from four experimental groups, gel-based mass spectrometry was then applied, and finally, 445 proteins were identified in pulled serum sample.

Results: Among these 445 proteins, 377 were present in at least one group from all. There were 35 proteins which were expressed when the duck was exposed to AFB1. The protein library that allows the identification of a large number of different proteins in duck serum will be enhanced by the addition of these peptide spectral data. It is noteworthy that chromodomain-helicase-DNA-binding protein 7 (CHD7) [Gallus gallus] was up-regulated in the group with the highest AFB1 contamination.

Conclusion: CHD7 protein might be somehow relative to aflatoxicosis in the duck that causes poor performance and economic loss. Moreover, other proteins present in duck serum were also added in the protein library.

Keywords: aflatoxin B1, duck, proteomics, serum.

Immune-inflammatory concept of the pathogenesis of chronic heart failure in dogs with dilated cardiomyopathy

Research (Published online: 28-09-2019)

21. Immune-inflammatory concept of the pathogenesis of chronic heart failure in dogs with dilated cardiomyopathy

Yu Vatnikov, A. Rudenko, P. Rudenko, Ev Kulikov, A. Karamyan, V. Lutsay, I. Medvedev, V. Byakhova, E. Krotova and M. Molvhanova

Veterinary World, 12(9): 1491-1498

ABSTRACT

Background: Dilated cardiomyopathy is common in dogs. This form of cardiomyopathy is the main cause of death due to heart disease in dogs. Death can occur suddenly in clinically normal animals as a result of the progression of congestive heart failure (CHF). The pathogenesis of heart failure syndrome in dogs with dilated cardiomyopathy involves activation of the neurohumoral system and immune-mediated inflammation, which leads to further progression of the condition. Heart failure syndrome in dogs with dilated cardiomyopathy is caused by the progressive loss of cardiomyocytes, apoptosis, remodeling of the left ventricle, systolic and diastolic dysfunction, arrhythmias, reduced cerebral blood flow, the involvement of other key internal organs, and intestinal dysbiosis.

Aim: This study aimed to determine the immunological and inflammatory mechanisms surrounding the development of heart failure syndrome in dogs with dilated cardiomyopathy.

Materials and Methods: The subjects of this study were dogs with a dilated form of cardiomyopathy (n=159), complicated by various functional classes of heart failure syndrome. Evaluation of myocardial remodeling, systolic function, and systemic hemodynamics was performed using EMP-860 Vet and PU-2200V ultrasound scanners according to the standard technique. Electrocardiography was performed with all dogs in right lateral recumbency using the EK1T-04 Midas electrocardiograph (50 mm/s speed and 1 mV gain = 1 cm).

Results: In some affected animals, especially in cases of compensated dilated cardiomyopathy, leukocytosis was noted. In patients with dilated cardiomyopathy complicated by heart failure syndrome of various functional classes, the number of neutrophils was significantly increased, and the number of lymphocytes was decreased by 1.9-2.1 times when compared with those in clinically normal animals. In dogs with dilated cardiomyopathy, neutrophilic leukocytosis develops with a simple regenerative shift to the left. The results of immunological studies indicate that dogs with dilated cardiomyopathy develop T lymphocytopenia as compared with clinically normal animals.

Conclusion: The central component of heart failure syndrome in dogs with dilated cardiomyopathy is the activation of the neurohumoral system and immune-mediated inflammation. The development of CHF in dogs with dilated cardiomyopathy is caused by the progressive loss of cardiomyocytes, apoptosis, remodeling of the left ventricle, systolic and diastolic dysfunction, arrhythmias, reduced cerebral blood flow, involvement of other key internal organs, and intestinal dysbiosis.

Keywords: dilated cardiomyopathy, dogs, pathogenesis, heart failure, immunity and inflammation.