Antibody immunoglobulin G1 and immunoglobulin G2a responses against some cystic fluid proteins of Cysticercus bovis in Balb/c mice

Research (Published online: 30-11-2018)

19. Antibody immunoglobulin G1 and immunoglobulin G2a responses against some cystic fluid proteins of Cysticercus bovis in Balb/c mice

I Nyoman Mantik Astawa, Ida Bagus Made Oka and I Made Dwinata

Veterinary World, 11(11): 1641-1647

ABSTRACT

Background and Aim: Immunoglobulin (Ig) G1 and IgG2a are the surrogate markers respectively for humoral and cellular immune responses of hosts against antigens including cystic fluid proteins of Cysticercus bovis. A study was conducted to investigate the IgG1 and IgG2a responses of Balb/c mice against some individual cystic fluid proteins of C. bovis in an effort to determine the roles of each protein in inducing the humoral and cellular immune responses in host.

Materials and Methods: Individual p71, p31, and p14 proteins of C. bovis were purified by separation of the proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and elution of individual proteins from the gel. Six female Balb/c mice were immunized 4 times at 10-day intervals with the crude cystic fluid proteins, and sera were collected for the measurement of IgG1 and IgG2a levels against the individual proteins. Sera samples collected before the first immunization were used as negative antibody control, sera samples collected after the fourth immunization were used as positive antibody control, and crude cystic fluid protein was used as positive antigen control.

Results: All immunized mice were immune to p71, p31, p14, and crude cystic fluid proteins of C. bovis. The crude cystic fluid proteins of C. bovis induced a higher IgG2a than IgG1 level following the first and the second immunizations but switched into a higher IgG1 than IgG2a level following the fourth immunization. Protein 71 kDa (p71) induced a higher IgG2a than IgG1 level following the fourth immunization. In contrast, p14 induced a higher IgG1 than IgG2a level following the fourth immunization. Low and balance IgG1 and IgG2a levels against p31 were observed following the first to the fourth immunizations.

Conclusion: Using IgG1 and IgG2a levels as the surrogate markers, it appears that cystic fluid antigens of C. bovis induce both humoral and cellular immune responses in Balb/c mice. The p71 appears to be a better inducer of cellular immune response, whereas p14 is a better inducer of humoral immune response of mice.

Keywords: Cystic fluid, Cysticercus bovis, immunoglobulin G1, immunoglobulin G2a, proteins.

Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Research (Published online: 29-11-2018)

18. Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Gusti Ayu Yuniati Kencana, Nyoman Suartha, I Made Kardena, Gusti Ayu Mayani Kristina Dewi, Arini Nurhandayani, Syamsidar and Kadek Karang Agustina

Veterinary World, 11(11): 1637-1640

ABSTRACT

Aim: The study was aimed to prepare and examine the potential and safety concerns of egg drop syndrome (EDS) vaccine candidate seed. The potential and safety trials of EDS Medan isolate vaccine need to be done before commercial scale of EDS vaccines are made.

Materials and Methods: The safety test of EDS candidate vaccine was tested on 4-week-old specified pathogen-free chickens in an experimentally isolated enclosure.

Results: The result of the safety test obtained 27.3 hemagglutination inhibition (HI) unit of geometric mean titer antibody post-vaccination. However, the potency test of the EDS candidate vaccine was conducted on 17-week-old laying hens. Test results of the EDS potency vaccine in layer obtained antibody titer increased in every week of blood taking with average titer of antibody: Before vaccinated was 22.9 HI unit, 1 week after vaccination was 23.7 HI unit, 2 weeks post-vaccination was 25 HI unit, and 3 weeks after vaccination was 27.3 HI units. In contrast, decreasing trend was observed in control group (unvaccinated chicken).

Conclusion: Serologically, the seed vaccine of EDS virus isolates from Medan was able to produce protective antibody titers starting in the 2nd and 3rd weeks post-vaccination.

Keywords: hemagglutination, inhibition test, Medan isolate, safety and potential test, seed of egg drop syndrome vaccine.

The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria

Research (Published online: 29-11-2018)

17. The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria

A. Lounas, K. Oumouna-Benachour, H. Medkour and M. Oumouna

Veterinary World, 11(11): 1630-1636

ABSTRACT

Background and Aim: Avian infectious bronchitis virus (IBV) frequently infects broilers and is responsible for severe economic losses to the poultry industry worldwide. It has also been associated with kidney damage in the broiler flocks. The aim of the present study is to determine the presence of IBV and its possible involvement in kidney damage of broiler chicks.

Materials and Methods: 14 clinically diseased broiler flocks from Western and Central Algeria were sampled and analyzed by hemagglutination inhibition (HI) test and reverse transcriptase-polymerase chain reaction (RT-PCR) followed by phylogenic analysis.

Results: The QX (100%) and 4/91 (60%) IBV serotypes were the most prevalent in the kidney damaged broilers regardless of vaccination status. The molecular detection of avian IBV by RT-PCR identified six samples as positive, of which only two isolates were typable by sequencing. We identified a novel IBDZ13a genotype which showed 93% sequence homology to the partial-S1 gene sequence of the IB 4/91 commercial vaccine strain. Sequencing analysis characterized this virus as a novel and divergent IB 4/91 field virus with eight amino acid substitutions that might have resulted in altered immunogenicity.

Conclusion: The isolation of a new IBV strain (IBDZ13a) from vaccinated broiler flocks may explain the failure of the vaccination programs against IBV field strains. Combination of the HI test and RT-PCR indicated that the nephropathogenic IB outbreaks in broilers are related to this novel strain.

Keywords: avian infectious bronchitis virus, broilers, kidney damages, Algeria, hemagglutination inhibition test, reverse transcriptase-polymerase chain reaction, IBDZ13a.

Effects of Artemisia herba-alba or olive leaf (Olea europaea) powder supplementation on growth performance, carcass yield, and blood biochemical parameters in broilers

Research (Published online: 26-11-2018)

16. Effects of Artemisia herba-alba or olive leaf (Olea europaea) powder supplementation on growth performance, carcass yield, and blood biochemical parameters in broilers

Asma Ait-Kaki, Mamadou Tandiang Diaw, Fikremariam Geda and Nassim Moula

Veterinary World, 11(11): 1624-1629

ABSTRACT

Aim: This study was aimed to evaluate the effects of Artemisia herba-alba (white wormwood) or olive leaf (Olea europaea) powder supplementation on growth performance, carcass yield, and serum biochemical parameters in broilers.

Materials and Methods: The study was conducted from April to May 2017 in Chemini region, Northern Algeria. A total of 60 1-day-old Ross 308 male chicks were divided into three groups consisted of 10 chicks, in each of two replications. The chicks in Group 1 were fed with a standard commercial diet (SCD); Group 2 received the same SCD with 2% supplementation of A. herba-alba powder; and Group 3 received the same SCD with 2% supplementation of O. europaea powder. Growth performance was measured with body weights every 2 weeks, daily feed intake, feed conversion ratio (FCR), and carcass yield at the end of 42 days of rearing. Blood samples were collected to analyze serum glucose, cholesterol, triglycerides, urea, and total protein levels.

Results: Results showed that, at 42 days of rearing, supplementation of O. europaea and A. herba-alba significantly increased (p<0.001) mean body weight (2230.10±26.38 g and 2117.42±26.38 g, respectively, vs. 2336.66±27.88 g in chicks of Group 1), but there was no significant difference (p≥0.05) among the three diets for FCR or percentage carcass yield. Among the serum biochemical parameters, glucose was significantly affected (p<0.01) by supplementation of olive leaf powder (1.90 g/L: Group 3), compared to the SCD (2.24 g/L: Group 1) or Artemisia powder (2.05 g/L: Group 2). Moreover, the supplementation of olive leaf powder in Group 3 broilers significantly affected (p<0.05) the serum cholesterol level (0.95 g/L), compared to the control diet (1.13 g/L). There was no significant difference (p≥0.05) for the other selected serum biochemical concentrations, namely triglycerides, urea, and total protein.

Conclusion: The supplementation of Artemisia or olive leaf powder into the diet for broilers improved body weight by about 5% or 10%, respectively, at slaughter with moderate changes in blood biochemical parameters.

Keywords: Artemisia herba-alba, broiler, growth, Olea europaea, serum parameters.

Role of transient receptor potential channels in regulating spermatozoa functions: A mini-review

Review (Published online: 23-11-2018)

15. Role of transient receptor potential channels in regulating spermatozoa functions: A mini-review

Akshay Kumar, Abhishek Kumar Mishra, Dilip Kumar Swain, Vijay Singh, Sarvajeet Yadav and Atul Saxena

Veterinary World, 11(11): 1618-1623

ABSTRACT

Flagellar navigation along the genital tract of male and female in spermatozoa is accomplished through a number of biological, physiological, biochemical, and electrophysiological alterations in spermatozoa. These alterations are highly precise, dynamic, and regulated through a number of ion channels along with their associated pathways. Beating of flagella along with intracellular metabolism of spermatozoa is associated with fluxing of Ca++ as well as release of Ca++ from different sources. Calcium fluxing through the spermatozoa is mediated through sperm-specific calcium channel and also through transient receptor potential (TRP) channels which are diversified multifamily of ion channels which are activated through a number of extracellular agents such as pH, temperature, chemicals, and pathogens. Research has shown the dynamic role of TRP channels in regulating sperm functions such as sperm chemotaxis, rheotaxis, thermotaxis, and eventually fertilization. Diversified forms of TRP and their involvement in regulation of sperm function opens new horizons of understanding of the sperm function and, in specific, issues related to infertility. This mini-review is an attempt to draw some insights into the action of TRP channels in regulating sperm fertility competence through both calcium-dependent and calcium-independent mechanisms.

Keywords: acrosome reaction, calcium, capacitation, fertilization, motility, pH, sperm, temperature, transient receptor potential.

Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients

Research (Published online: 22-11-2018)

14. Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients

Sri Rahmatul Laila, Dewi Apri Astuti, Irma Herawati Suparto, Ekowati Handharyani and Dondin Sajuthi

Veterinary World, 11(11): 1609-1617

ABSTRACT

Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys.

Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment.

Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT.

Conclusion: The IPB-1 atherogenic diet formulated with locally and readily available ingredients provides an economically and scientifically feasible monkey model to study atherosclerosis in Indonesia and Southeast Asia.

Keywords: atherogenic diet, carotid artery, cynomolgus, ultrasonography.

Association between milk urea nitrogen and first service conception in smallholder dairy farms under heat and humidity stress

Research (Published online: 22-11-2018)

13. Association between milk urea nitrogen and first service conception in smallholder dairy farms under heat and humidity stress

Suppada Kananub, John A. VanLeeuwen and Pipat Arunvipas

Veterinary World, 11(11): 1604-1608

ABSTRACT

Aim: The study was to evaluate the relationship between the first service conception (FSC) and milk urea nitrogen (MUN) in smallholder dairy farms under heat and humidity stress.

Materials and Methods: Dairy cows from 43 dairy farms giving birth between November 2014 and April 2015 (n=295) contributed to the study. All cows were sampled monthly to measure milk compositions, and we collected additional farm data from farmers through a questionnaire. The first service during the first 120 days of lactation was the outcome of interest in this study. Multivariable logistic regression determined significant associations with FSC.

Results: The overall FSC was 22% and the mean MUN concentration was 11.55 mg/dl. The final FSC model included MUN concentration, the season of breeding, and protein energy ratio (PE ratio) in the diet. The odds of FSC were reduced by approximately 10% for each mg/dl higher MUN on the day of the milk sample that was nearest to the artificial insemination (AI) day. The odds of FSC were nearly 3 times higher when the first insemination occurred in winter compared to summer first services. Taking into account the nutritional factors, the odds of FSC were nearly 70% higher with an increase in PE ratio of 10 g of crude protein/Mcal from the mean of 35.90 g.

Conclusion: This study of smallholder dairy farmers in the hot and humid climate of Thailand confirmed that season, nutritional management, and MUN concentration were associated with FSC. MUN appears to be a useful indicator to monitor the effects of diet on reproductive performance from this study.

Keywords: first service conception, milk urea nitrogen, smallholder farm.