Some hematological and biochemical parameters of different goat breeds in Sultanate of Oman "A preliminary study"

Research (Published online: 30-04-2017)

15. Some hematological and biochemical parameters of different goat breeds in Sultanate of Oman "A preliminary study"

Shahab Al-Bulushi, Turke Shawaf and Afaf Al-Hasani

Veterinary World, 10(4): 461-466

ABSTRACT

Aim: In Sultanate of Oman, goats are considered as one of the most important livestock in which there are many breeds of goat such as Batinah, Jabali, Dhofari, Jabal Al-Akhdar, Sahrawi, and Sahrawi Musandam. Little hematological and biochemical information is known on Omani goat breeds; therefore, the main purpose of this study was to determine reference baseline data regarding hematological and biochemical values of different Omani goat breeds.

Materials and Methods: A total of 30 healthy animals of different Omani goat breeds (Jabali, Jabal Al-Akhdar, Sahrawi, and Sahrawi Musandam) were selected randomly from different areas in Sultanate of Oman. The blood samples were collected from the jugular vein into two tubes for blood hematology and biochemical analysis. Statistical analysis was applied by using GraphPad Prism 7 software to calculate the minimum and maximum values to determine the range, mean, standard deviation of the mean and the p value.

Results: No statistically significant variation in most hematological and biochemical parameters was found among the Omani goat breeds. The results of blood hematology revealed that the mean white blood cells (14.6±3.32 x103/μL), and the percentage of neutrophils in Omani goats (60.87±8.46%) were higher than that in most goat breeds. Higher values of red blood cells (12.8±1.28 x106/μL), hemoglobin (10.4±1.92 g/dl), hematocrit (38.29±4.06%), and lower values of mean corpuscular HGB concentration (27.05±3.5 g/dl) were observed in Omani goat breeds comparing to that in the other goat breeds. Lower values of total bilirubin (0.22±0.03 mg/dl), blood urea nitrogen (14.62±2.66 mg/dl), and cholesterol (48.58±19.05 mg/dl) were found in Omani goat breeds when compared to that of the other goat breeds.

Conclusion: The obtained results are considered as the first values to be published for the different Omani goat breeds. This study is considered as preliminary study which can be used as a reference for further studies to determine reference values for the studied breeds to aid the veterinarians in the interpretation of the laboratory data and for the selection of the appropriate treatment.

Keywords: biochemical, breeds, goat, hematological, parameters, Omani.

Exploring the potential hazard of Mycobacterium avium subspecies paratuberculosis as a cause for Crohn's disease

Research (Published online: 28-04-2017)

14. Exploring the potential hazard of Mycobacterium avium subspecies paratuberculosis as a cause for Crohn's disease

Sukumar Bharathy, Lakshmanasami Gunaseelan and Kannan Porteen

Veterinary World, 10(4): 457-460

ABSTRACT

Background: Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease (JD), or paratuberculosis in ruminants has been suspected to be implicated in the pathogenesis of Crohn's disease (CD) in humans with chronic inflammatory intestinal changes. As the hypothesis is now fast being recognized that MAP could possibly be the etiological agent of CD which is found to be excreted in milk of dairy animals subclinically or terminally ill with JD.

Aim: The present study was aimed to detect MAP in milk by polymerase chain reaction (PCR) targeting IS900 and to describe the excretion pattern of MAP in milk from asymptomatic lactating cows and does with relevance to the public health significance.

Materials and Methods: A total of 77 milk samples were collected randomly from lactating animals which include cows (45) and does (32). All the 77 milk samples were processed to identify the presence of MAP by employing the direct IS900 PCR as per the standard protocol.

Results: Out of 77 milk samples from asymptomatic lactating animals, 12 (15.58%) were showed positivity for IS900 PCR in which 5 (11.11%) were from lactating cows and 7 (21.87%) were from lactating does.

Conclusion: In our study, 15.58% of milk samples showed IS900 positivity which indicates the presence of subclinical MAP infection in lactating animals. Hence, there is a possibility for excretion of MAP through milk which can be a potential threat for CD in humans by raw milk consumption. Therefore, the prevention of MAP in the food chain need to be assured by sourcing raw products from animal herds free of MAP infection.

Keywords: Crohn's disease, excretion, food chain, IS900 polymerase chain reaction, Mycobacterium avium subspecies paratuberculosis, raw milk.

Spatial pattern of foot-and-mouth disease virus serotypes in North Central Nigeria

Research (Published online: 26-04-2017)

13. Spatial pattern of foot-and-mouth disease virus serotypes in North Central Nigeria

Yiltawe Simwal Wungak, Olayinka O. Ishola, Babasola O. Olugasa, David D. Lazarus, David O. Ehizibolo and Hussaini G. Ularamu

Veterinary World, 10(4): 450-456

ABSTRACT

Aim: This study aimed to determine the foot-and-mouth disease virus (FMDV) serotypes circulating, the prevalence of FMDV serotypes, and the spatial distribution of FMDV among sedentary and pastoral cattle herds in the North-Central Nigeria.

Materials and Methods: A cross-sectional study was undertaken, during which a total of 155 sera that tested positive for foot-and-mouth disease (FMD) 3ABC non-structural protein antibodies were selected and screened for FMD structural protein serotypes, A, O, SAT 1, and SAT 2 using a solid-phase competitive enzyme-linked immunosorbent assay (ELISA). Epithelial tissue specimens were collected during outbreak investigations which were tested for FMD using an antigen capture ELISA for serotype A, O, SAT 1, and SAT 2.

Results: An overall serotype-specific prevalence of 79.35 (95% confidence interval [CI]: 72.4-85.18) was recorded for serotype O, 65.2% (95% CI: 57.41-72.3) for serotype A, 52.9% (95% CI: 45.03-60.67) for SAT-2, and 33.55% (95% CI: 26.45-41.26) for SAT-1. Evidence of exposure to multiple FMDV serotypes showed that 12.26% of the sera samples had antibodies against four serotypes circulating, 30.97% had antibodies against three serotypes circulating, 22.58% had antibodies against two serotypes, and 17% showed exposure to only one serotype. Clinical specimens (epithelial tissue) collected during outbreak investigations showed that serotype O has the highest proportion of 50% with serotype A - 25%; SAT 2 - 20.8%; and SAT 1 - 4.1%.

Conclusion: The study detected diffuse and co-circulation of serotypes A, O, SAT1, and SAT2 within the study area, and hence the need for the appropriately matched multivalent vaccine is strongly advocated for FMD control in Nigeria.

Keywords: antibodies, endemic, foot-and-mouth disease, multiple, Nigeria, serotypes, spatial.

The importance of timely introduction of vancomycin therapy against methicillin-resistant Staphylococcus aureus (MRSA) bacteremia and severity of MRSA bacteremia at Teaching Hospital, Anuradhapura, Sri Lanka

Research (Published online: 25-04-2017)

2. The importance of timely introduction of vancomycin therapy against methicillin-resistant Staphylococcus aureus (MRSA) bacteremia and severity of MRSA bacteremia at Teaching Hospital, Anuradhapura, Sri Lanka -Jayaweera Arachchige Asela Sampath Jayaweera, Malika Karunarathne and Wikum Widuranga Kumbukgolla

International Journal of One Health, 3: 7-11

  doi: 10.14202/IJOH.2017.7-11

Abstract

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Aim: Worldwide, an estimated 2 billion healthy people carry Staphylococcus aureus (SA) and of these, up to 53 million are thought to carry methicillin-resistant SA (MRSA). MRSA bacteremia patients are more critical to manage and timely introduction of antibiotics is life-saving. The aim of the study was to elucidate the prevalence of MRSA bacteremia in different units of Teaching Hospital, Anuradhapura (THA), Sri Lanka and assess the clinical characteristics and associated mortality related to timely introduction of vancomycin therapy.

Materials and Methods: The data on MRSA bacteremia which were obtained from THA, for the period of March 2012 to December 2013 were statically analyzed emphasizing the unit-wise prevalence, severity, and comorbidity and timely introduction of vancomycin therapy.

Results: The laboratory records of total 13,260 blood cultures were analyzed. Of those, MRSA bacteremia was detected in 61 cultures (9.3%). The highest prevalence of MRSA bacteremia was observed in the nephrology unit. The survival rate of the patients when the vancomycin therapy started before 24 h of receiving the blood culture report was 94.9% and in the instances of the treatment started after 24 h of blood culture report, the survival rate decreased down to 50%. High Pitt Bacteraemia score (PBS) (p<0.05) and initiation of vancomycin therapy after 24 h following the receipt of blood culture report (p<0.05) independently affected the MRSA bacteremic patient’s 7th day mortality. Having comorbidities have not shown significant impact on 7th day mortality.

Conclusion: The start of vancomycin therapy as earlier as possible following arrival of antibacterial susceptibility test reduces the likelihood of mortality.

Keywords: MRSA bacteremia, timing of vancomycin, severity.

Molecular detection of Taenia spp. in dogs' feces in Zanjan Province, Northwest of Iran

Research (Published online: 23-04-2017)

12. Molecular detection of Taenia spp. in dogs' feces in Zanjan Province, Northwest of Iran

Mohammad Hasan Kohansal, Abbasali Nourian, Ali Haniloo and Asghar Fazaeli

Veterinary World, 10(4): 445-449

ABSTRACT

Aim: Echinococcus and Taenia spp. are important but neglected zoonotic helminths of dogs. Dogs as the most relevant definitive hosts harbor several species of Taenia and Echinococcus simultaneously in their gastrointestinal lumen which are morphologically indistinguishable. In this study, we used a multiplex polymerase chain reaction (PCR) method to identify Taeniid infections which seem to be highly distributed in the study region.

Materials and Methods: A total of 450 dog fecal samples were collected from eight different areas of Zanjan province, northwest of Iran, and examined using a flotation method followed by multiplex PCR for detection and identification of parasites' eggs.

Results: Gastrointestinal parasites were found in 86 out of 450 fecal samples (19.1%) by microscopic examination. Taeniid eggs were observed in 5.6% of samples, containing 0.45%, 3.8%, and 1.3% Echinococcus granulosus, Taenia spp., and mix infection of both E. granulosus and Taenia spp., respectively. Echinococcus multilocularis was absent in the samples.

Conclusion: A relatively low rate of E. granulosus (1.8%) was observed in this study. However, risks of this parasite should not be overlooked, and control programs need to be extended for this species and other Taeniid spp. In particular, dogs are recommended to be dewormed more frequently.

Keywords: dog, Echinococcus spp., eggs, multiplex polymerase chain reaction, Taenia spp.

Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia

Research (Published online: 22-04-2017)

11. Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia

Dwi Desmiyeni Putri, Ekowati Handharyani, Retno Damajanti Soejoedono, Agus Setiyono, Ni Luh Putu Ika Mayasari and Okti Nadia Poetri

Veterinary World, 10(4): 438-444

ABSTRACT

Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.

Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.

Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R/G-F117 on NDV virulent strains and 112G-K/R-Q-G-R-L117 on NDV avirulent strain.

Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.

Keywords: F gene, M gene, molecular pathotyping, Newcastle disease virus, vaccinated chickens, virulence.

Prospects of complete feed system in ruminant feeding: A review

Review (Published online: 21-04-2017)

10. Prospects of complete feed system in ruminant feeding: A review

Yasir Afzal Beigh, Abdul Majeed Ganai and Haidar Ali Ahmad

Veterinary World, 10(4): 424-437

ABSTRACT

Effective utilization of available feed resources is the key for economical livestock rearing. Complete feed system is one of the latest developments to exploit the potential of animal feed resources in the best possible way. The complete feed is a quantitative mixture of all dietary ingredients, blended thoroughly to prevent separation and selection, fed as a sole source of nutrients except water and is formulated in a desired proportion to meet the specific nutrient requirements. The concentrate and roughage levels may vary according to the nutrient requirement of ruminants for different production purposes. The complete feed with the use of fibrous crop residue is a noble way to increase the voluntary feed intake and thus animal's production performance. In this system of feeding, the ruminant animals have continuous free choice availability of uniform feed mixture, resulting in more uniform load on the rumen and less fluctuation in release of ammonia which supports more efficient utilization of ruminal non-protein nitrogen. Feeding complete diet stabilizes ruminal fermentation, thereby improves nutrient utilization. This feeding system allows expanded use of agro-industrial byproducts, crop residues and nonconventional feeds in ruminant ration for maximizing production and minimizing feeding cost, thus being increasingly appreciated. However, to extend the concept extensively to the field and make this technology successful and viable for farmers, more efforts are needed to be taken.

Keywords: benefits, complete feed, concept, feeding, ruminants.