Incidence and virulence characteristics of Aeromonas spp. in fish

Research (Published online: 12-01-2017)

7. Incidence and virulence characteristics of Aeromonas spp. in fish - Ashraf M. Abd-El-Malek

Veterinary World, 10(1): 34-37

   doi: 10.14202/vetworld.2017.34-37

Abstract

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Aim: This study was conducted to evaluate the presence of Aeromonas spp. in raw and ready-to-eat (RTE) fish commonly consumed in Assiut city, Egypt, and to determine virulence factors due to they play a key role in their pathogenicity.

Materials and Methods: A total of 125 samples of raw and RTE fish samples were taken from different fish markets and fish restaurants in Assiut Governorate and screened for the presence of Aeromonas spp. by enrichment on tryptic soy broth then incubated at 30°C for 24 h. Plating unto the sterile Petri dishes containing Aeromonas agar base to which Aeromonas selective supplement was added. The plates were incubated at 37°C for 24 h. Presumptive Aeromonas colonies were biochemically confirmed and analyzed for pathogenicity by hemolysin production, protease, and lipase detection.

Results: The results indicated that raw fish were contaminated with Aeromonas spp. (40% in wild and 36% in cultured Nile tilapia). Regarding RTE, Aeromonas spp. could be isolated with the percentage of 16%, 28% and 20% in fried Bolti, grilled Bolti and fried Bayad, respectively. Out of 35 isolates obtained, 22 were categorized as Aeromonas hydrophila, 12 were classified as Aeromonas sobria and Aeromonas caviae were found in only one isolate. The virulence factors of Aeromonas spp. were detected and the results showed that all isolates produced of hemolysin (91.4%), protease (77.1%), and lipase enzyme (17.1%).

Conclusion: This study indicates that the presence of A. hydrophila with virulence potential in fresh and RTE fish may be a major threat to public health.

Keywords: Aeromonas hydrophila, fish, hemolysin, lipase, protease enzyme, public health.

Prevalence of colibacillosis in chickens in greater Mymensingh district of Bangladesh

Research (Published online: 11-01-2017)

6. Prevalence of colibacillosis in chickens in greater Mymensingh district of Bangladesh - Md. Abdul Matin, Md. Ariful Islam and Mst. Minara Khatun

Veterinary World, 10(1): 29-33

   doi: 10.14202/vetworld.2017.29-33

Abstract

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Aim: This study was conducted for determination of the prevalence of colibacillosis in chicken in poultry farms in Mymensingh and Tangail districts. Isolation, identification, and antibiogram profile of Escherichia coli were also performed.

Materials and Methods: A total of 25 chickens manifested clinical signs of colibacillosis were collected from five different poultry farms during natural outbreaks.

Results: In broiler, the prevalence of colibacillosis was 0.84%, and in layer, prevalence was 0.80%. The prevalence of colibacillosis was 1.0% and 0.5% in 25-30 days old and 31-35 days old broiler, respectively. In case of layer birds, the prevalence was 0.6% in 40-45 days old bird and 1% in 46-50 days old bird. Identity of the E. coli isolate of chicken was confirmed by sugar fermentation, biochemical tests, and polymerase chain reaction assay. Antibiogram profile of E. coli isolate of chicken revealed that it was multidrug resistant (resistant against two antibiotics, such as ampicillin and cefalexin).

Conclusion: Data of this study suggest that colibacillosis is prevalent in the study areas which underscore the need of implementation of prevention and control measure against this disease.

Keywords: chicken, colibacillosis, multidrug resistant Escherichia coli, Mymensingh, Tangail.

M-mode echocardiographic reference values in Pantja goats

Research (Published online: 10-01-2017)

5. M-mode echocardiographic reference values in Pantja goats - Parul Singh, Narendra Singh Jadon, Deepti Bodh and Manjul Kandpal

Veterinary World, 10(1): 22-28

   doi: 10.14202/vetworld.2017.22-28

Abstract

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Aim: The aim of this study was to establish M-mode echocardiographic reference values in Pantja goats and to study the effect of gender and body weight (BW) on these parameters.

Materials and Methods: A total of 18, clinically healthy, adult Pantja goats of either sex, aged 2-4 years and weighing 10-44 kg were included in the study. Echocardiographic examination was performed in the standing unsedated animal. All measurements were made from the right parasternal long-axis left ventricular outflow tract view of the heart. The following parameters were recorded: Left ventricular internal diameter at diastole and systole, interventricular septal thickness at diastole and systole, left ventricular posterior wall (LVPW) thickness at diastole and systole, end diastolic and systolic volumes, stroke volume, fractional shortening, ejection fraction, percent systolic thickening of interventricular septum, percent systolic thickening of LVPW, cardiac output, left atrial (LA) diameter at diastole and systole, aortic (AO) root diameter at diastole and systole, LA/AO, LA posterior wall thickness at diastole and systole, left ventricular ejection time, DE amplitude, EF slope, AC interval and e-point to septal separation.

Results: This study demonstrated specific reference ranges of M-mode echocardiographic parameters and indices in healthy Pantja goats. Normal echocardiographic values obtained in Pantja goats were quite different from other goat breeds. Gender had no influence on echocardiographic parameters, while high correlations were found between most echocardiographic parameters and BW.

Conclusion: The echocardiographic values obtained in the study may serve as a reference for future studies in this breed, for cardiovascular disease diagnosis and for utilizing the goat as a model for cardiac disorders in humans.

Keywords: echocardiography, goats, heart disease, M-mode, Pantja.

Study of the dairy characters of lactating Murrah buffaloes on the basis of body parts measurements

Research (Published online: 09-01-2017)

4. Study of the dairy characters of lactating Murrah buffaloes on the basis of body parts measurements - Sandeep Dhillod, Dipankar Kar, C. S. Patil, Subhasish Sahu and Narender Singh

Veterinary World, 10(1): 17-21

   doi: 10.14202/vetworld.2017.17-21

Abstract

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Aim: The aim of the study was to correlate the milk yield of Murrah buffaloes with certain body parts measurements.

Materials and Methods: A total of 70 lactating Murrah buffaloes were selected from Buffalo Farm, Lala Lajpat Rai University of Veterinary and Animal Science, Hisar and were randomly selected in a range from first to fifth parity. Traits studied were 305 days milk yield (MY), body weight (BW), body length (BL), muzzle width (MW), height at wither (HW), abdominal girth (AG), chest girth (CG), body depth fore, body depth rear, hip bone distance (HBD), pin bone distance (PBD), skin thickness (STK), and tail length (TL). Data were collected and statically analyzed by Pearson’s correlation method.

Result: The result of this study showed that Murrah buffaloes had the average 2604.8±39.5 kg for MY, 556.1±4.9 kg for BW, and 152.2±0.8 cm for BL. This study showed that buffaloes had positive significant (p<0.05) correlation between MY and BW (0.26). Highly significant (p<0.01) correlation was observed between MY and AG (0.64), MW (0.42). Significant (p<0.01) negative correlation was observed between MY and STK (−0.79). Different body part measurements (BW, BL, HW, AG, CG, MW, TL, BD, PBD, HBD, STK) were significantly correlated with each other.

Conclusion: This study can be helpful as a selection tool to enhance and evaluate the production potential by setting standards of Murrah buffalo breed. BW, abdominal growth, muzzle thickness, and STK were found key factors while selecting a dairy Murrah buffalo.

Keywords: body parts measurements, correlation, dairy characters, regression.

Effects of different vegetable oils on rumen fermentation and conjugated linoleic acid concentration in vitro

Research (Published online: 08-01-2017)

3. Effects of different vegetable oils on rumen fermentation and conjugated linoleic acid concentration in vitro - Amitava Roy, Guru Prasad Mandal and Amlan Kumar Patra

Veterinary World, 10(1): 11-16

   doi: 10.14202/vetworld.2017.11-16

Abstract

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Aim: The objective of this study was to investigate the effect of different vegetable oils on rumen fermentation and concentrations of beneficial cis-9 trans-11 C18:2 conjugated linoleic acid (CLA) and trans-11 C18:1 fatty acid (FA) in the rumen fluid in an in vitro condition.

Materials and Methods: Six vegetable oils including sunflower, soybean, sesame, rice bran, groundnut, and mustard oils were used at three dose levels (0%, 3% and 4% of substrate dry matter [DM] basis) in three replicates for each treatment in a completely randomized design using 6 × 3 factorial arrangement. Rumen fluid for microbial culture was collected from four goats fed on a diet of concentrate mixture and berseem hay at a ratio of 60:40 on DM basis. The in vitro fermentation was performed in 100 ml conical flakes containing 50 ml of culture media and 0.5 g of substrates containing 0%, 3% and 4% vegetable oils.

Results: Oils supplementation did not affect (p>0.05) in vitro DM digestibility, and concentrations of total volatile FAs and ammonia-N. Sunflower oil and soybean oil decreased (p<0.05) protozoal numbers with increasing levels of oils. Other oils had less pronounced effect (p>0.05) on protozoal numbers. Both trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations were increased (p<0.05) by sunflower and soybean oil supplementation at 4% level with the highest concentration observed for sunflower oil. The addition of other oils did not significantly (p>0.05) increase the trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations as compared to the control. The concentrations of stearic, oleic, linoleic, and linolenic acids were not altered (p>0.05) due to the addition of any vegetable oils.

Conclusion: Supplementation of sunflower and soybean oils enhanced beneficial trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations in rumen fluid, while sesame, rice bran, groundnut, and mustard oils were ineffective in this study.

Keywords: conjugated linoleic acid, goat, rumen fluid, vaccenic acid, vegetable oil.

Molecular characterization of Rhodococcus equi isolates in equines

Research (Published online: 05-01-2017)

2. Molecular characterization of Rhodococcus equi isolates in equines - Rabyia Javed, A. K. Taku, R. K. Sharma and Gulzaar Ahmed Badroo

Veterinary World, 10(1): 6-10

   doi: 10.14202/vetworld.2017.6-10

Abstract

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Aim: The aim was to determine the occurrence of Rhodococcus equi in equines and their environment in Jammu (R.S. Pura, Katra), molecular characterization and to determine the antibiotic resistance pattern of R. equi.

Materials and Methods: A total of 96 nasopharyngeal swab samples were collected from equines. The organism was isolated on Columbia nalidixic acid agar containing 5% sheep blood as well as on sheep blood agar and was later confirmed by cultural characteristics and biochemical tests. Molecular detection of R. equi isolates was done by 16S rRNA gene amplification followed by virulence associated protein A (Vap A) gene amplification. Antibiogram was performed against five antibiotics, viz., amoxicillin, penicillin G, streptomycin, rifampicin, and methicillin.

Results: During the study, 9 R. equi isolates were identified on the basis of cultural and biochemical tests. In the polymerase chain reaction based detection, 3 among the 9 rhodococcal isolates were positive for species-specific 16S rRNA gene and revealed amplicon of 450 bp for confirmation of 16S rRNA gene. None of the sample was found positive for Vap A gene. In antibiogram, R. equi isolates were found sensitive for amoxicillin, while some isolates were also found resistant to the most conventional antibiotic penicillin G.

Conclusion: From this study, it was concluded that R. equi infection is prevalent in equines in Jammu region of India and the indiscriminate use of the antibiotics is leading toward the development of resistant strains of R. equi.

Keywords: 16S rRNA, polymerase chain reaction, Rhodococcus equi.

Identification of suitable housing system for dairy cattle in North East Zone of Tamil Nadu, India, with respect to microclimate

Research (Published online: 04-01-2017)

1. Identification of suitable housing system for dairy cattle in North East Zone of Tamil Nadu, India, with respect to microclimate - 

T. Sivakumar, P. T. Suraj, A. Yasotha and Jayashree Phukon

Veterinary World, 10(1): 1-5

   doi: 10.14202/vetworld.2017.1-5

Abstract

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Aim: To identify the suitable roofing pattern for dairy cattle in North East Zone of Tamil Nadu, India, based on micro climatic conditions.

Materials and Methods: Initially, survey was conducted to identify and categorize the major housing patterns existing in the region for further detailed investigation. In total, 30 farmers/farms consisting of five housing types with six replicates were selected. Temperature and temperature humidity index (THI) were recorded using the maximum-minimum thermometer and digital thermo-hygrometers. The study was conducted for 1 year covering four seasons namely South West monsoon (June-August), North East monsoon (September-November), cold season (December-February), and summer season (April-May). The data were statistically analyzed using statistical package SPSS 17.

Results: Animal shelters with cement sheets recorded the highest temperature (26.71±1.13°C) and THI (77.23±1.76) at 8.00 am, whereas the lowest temperature (24.83±1.17°C) and THI (74.54±1.72) were recorded in the thatched shed. There was significant difference (p<0.01) in temperature and THI at 8.00 am during South West monsoon and North East monsoon seasons between the housing types. During cold and summer seasons, there was no significant difference (p≥0.05) in the environmental variables among various shelter systems.

Conclusion: Thatched housing is found to be the suitable one with respect to the climatic variables, followed by tile roof and metal roof. The cement sheet roofed housing is found to be the most unsuitable one in the region for dairy cattle.

Keywords: dairy cattle, micro-climate, roofing pattern.