Stimulating immunoglobulin response by intramuscular delivery of exopolysaccharides-adjuvanted mannheimiosis vaccine in goats

Research (Published online: 28-12-2022)

25. Stimulating immunoglobulin response by intramuscular delivery of exopolysaccharides-adjuvanted mannheimiosis vaccine in goats

Ghaith Hussein Mansour, Laith Abdul Razzak, A. Suvik, and Mohd Effendy Abd. Wahid

Veterinary World, 15(12): 2945-2952

ABSTRACT

Background and Aim: Pneumonic mannheimiosis (PM) is a common respiratory bacterial disease among small ruminants. Despite numerous management methods, vaccination remains a suitable strategy to combat or reduce PM in goats and sheep. Thus, a study was conducted in Malaysia to evaluate the immunogenicity of exopolysaccharide-adjuvanted Mannheimia haemolytica A2 vaccine (EPS-MHA2) under laboratory and field conditions for its potential use as an efficient vaccine against PM.

Materials and Methods: This study induced immunoglobulin (Ig) responses following intramuscular (IM) delivery of the EPS-MHA2 vaccine on 12 goats for about 7 months. Goats were divided into three groups, with three goats per group, and they were vaccinated intramuscularly as follows: Group 1 was vaccinated with an adjuvanted vaccine prepared from formalin-killed M. haemolytica serotypes A2 and EPS excipient; Group 2 was vaccinated with formalin-killed M. haemolytica seed only, whereas Group 3 was injected with phosphate-buffered saline (PBS) as the negative control. Measures of specific immunity included serum IgM, IgG, and IgA as well as bronchoalveolar lavage fluid secretory IgA and the size and number of the bronchus-associated lymphoid tissue (BALT).

Results: From the 1st day of vaccination, Groups 1 and 2 showed a significant (p < 0.05) increase in serum IgM, IgG, and IgA levels. However, the antibodies started to decline 5-week post-vaccination, indicating that the booster dose was necessary. On the second exposure to the same vaccine (booster), the level of antibodies showed a significant increase (p < 0.05), particularly IgG. All groups were challenged intratracheally by virulent MHA2 2 weeks after the decline of second antibodies on the administration of booster. All goats were euthanatized and necropsied 4-week post-challenge. The number and size of the BALT in Group 1 goats significantly increased compared with those in Group 2 and the unvaccinated control. Bacteriological parameters were evaluated, in which MHA2 was reisolated successfully from lung samples in Group 3. The IgA level produced by the group vaccinated with EPS-MHA2 was significantly (p < 0.001) higher than that the MHA2 vaccine and PBS groups. All data obtained were analyzed statistically using a one-way analysis of variance. The results indicate that IM injection of EPS-MHA2 vaccine significantly enhanced the immune response against MHA2.

Conclusion: Therefore, the addition of EPS to MHA2 (EPS-MHA2 vaccine) can effectively protect goats from lethal mannheimiosis infection. Factors such as the ideal concentration of EPS should be further studied to verify its application potential as a vaccine adjuvant, and the extraction of EPS from different microalgae species should be further investigated. This study showed a novel and exciting set of data and a vaccination system, in which the suppressive effects of mannheimiosis may be further investigated.

Keywords: adjuvant, immunoglobulin, Mannheimia Haemolytica, mannheimiosis, vaccine.

A urinary proteomic study in hypercalciuric dogs with and without calcium oxalate urolithiasis

Research (Published online: 27-12-2022)

24. A urinary proteomic study in hypercalciuric dogs with and without calcium oxalate urolithiasis

Sumonwan Chamsuwan, Chollada Buranakarl, Kris Angkanaporn, Thasinas Dissayabutra, Natthaya Chuaypen, Trairak Pisitkun, and Nuttiya Kalpongnukul

Veterinary World, 15(12): 2937-2944

ABSTRACT

Background and Aim: Hypercalciuria is an important predisposing factor commonly found in humans and dogs with calcium oxalate (CaOx) urolithiasis. Calcium oxalate crystals can induce an inflammatory reaction that subsequently produces several proteins that have an inhibitory or stimulatory effect on stone formation. This study aimed to evaluate the differences in urinary proteomic profiles between hypercalciuric CaOx stone dogs and hypercalciuric stone-free dogs (CaOx stone and control groups, respectively).

Materials and Methods: Seven dogs with hypercalciuric CaOx urolithiasis and breed-, sex-, and aged-matched controls with hypercalciuria were included in the study. Serum and urine samples were obtained from all dogs to analyze electrolytes. Urinary proteomic profiles were analyzed using liquid chromatography-mass spectrometry. Student's t-test was used to compare the differences between groups.

Results: Forty-nine urinary proteins were identified in the stone-free and CaOx stone groups, whereas 19 and 6 proteins were unique in the CaOx stone and stone-free groups, respectively. The urinary thrombomodulin level was significantly higher in the CaOx stone group (relative ratio = 1.8, p < 0.01) than in the stone-free group.

Conclusion: This study demonstrated that urinary proteomic profiles may be used as a candidate biomarker for urinary tract injury in CaOx urolithiasis in dogs.

Keywords: calcium oxalate stone, dog, hypercalciuria, urinary biomarker.

Intraocular pressure and cardiovascular effects of dexmedetomidine premedication and tiletamine-zolazepam for anesthetic induction in dogs

Research (Published online: 27-12-2022)

23. Intraocular pressure and cardiovascular effects of dexmedetomidine premedication and tiletamine-zolazepam for anesthetic induction in dogs

Pradipa Kusolphat, Tanawan Soimala, and Jutapoln Sunghan

Veterinary World, 15(12): 2929-2936

ABSTRACT

Background and Aim: The effect of anesthetic drugs on intraocular pressure (IOP) is an important concern in ophthalmic surgery. The impact of dexmedetomidine (DEX) combined with tiletamine-zolazepam on IOP is scarcely studied. This study aimed to evaluate IOP and cardiovascular effects in dogs after premedication with 5 μg/kg (DEX5) or 10 μg/kg (DEX10) of intramuscular DEX followed by intravenous tiletamine-zolazepam administration for induction of anesthesia in healthy dogs.

Materials and Methods: Eighteen dogs, American Society of Anesthesiologists I or II, without ocular abnormality were investigated. All dogs were randomly divided into the DEX5 (n = 9) and DEX10 groups (n = 9). The IOP, heart rate (HR), systolic blood pressure (SBP), oxygen saturation, and sedation scale were measured before premedication (baseline), after premedication at 5, 10, 15, and 20 min, after tiletamine-zolazepam administration, after endotracheal intubation, and post-operative.

Results: There were no significant differences between the groups at any time point. The DEX5 and DEX10 groups had significantly decreased HR values at 10 min compared with baseline. The IOP at 20 min was significantly lower compared to the baseline in the DEX10 group. Moreover, the DEX10 group showed increased IOP, HR, SBP, and sedation scale values after induction and intubation compared with 20 min, but these values did not differ significantly from baseline. All parameters of both groups did not change significantly between post-operative and baseline.

Conclusion: Intramuscular DEX (10 μg/kg) is an appropriate premedication in ophthalmic examination or surgical procedures. Moreover, it could be combined with tiletamine-zolazepam for generalized anesthesia in dogs with an ophthalmic problem, as it had no clinically significant effects on IOP or cardiovascular values.

Keywords: cardiovascular, dexmedetomidine, intraocular pressure, tiletamine-zolazepam.

Native microbiomes in danger: Could One Health help to cope with this threat to global health?

Review (Published online: 26-12-2022)

9. Native microbiomes in danger: Could One Health help to cope with this threat to global health?

Pablo Zunino

International Journal of One Health, 8(2): 178-184

ABSTRACT

Planetary health faces an emergency associated with global change. Climate change, the increase in world population and urban concentration, the hyperintensification of productive systems, and the associated changes in land use, among other factors, are generating a risky substrate for global health deterioration. The emergence of the coronavirus disease 2019 pandemic is an example of the problems that this situation can provoke. Several researchers and health professionals have addressed the role of microorganisms, particularly bacteria, in promoting global health, mainly in the past decades. However, global change has contributed to the extinction of a wide array of bacterial species and the disruption of microbial communities that support the homeostasis of humans, animals, and the environment. The need to protect the diversity and richness of native microbiomes in biotic and abiotic environments is crucial but has been frequently underestimated. The "One Health" approach, based on integrating traditionally unconnected fields such as human, animal, and environmental health, could provide a helpful framework to face this challenge. Anyway, drastic political decisions will be needed to tackle this global health crisis, in which the preservation of native microbial resources plays a critical role, even in preventing the risk of a new pandemic. This review aims to explain the importance of native microbiomes in biotic and abiotic ecosystems and the need to consider bacterial extinction as a crucial problem that could be addressed under a One Health approach.

Keywords: bacteria, global change, health, microbiome, One Health.

The prevalence and antibiotic susceptibility of Staphylococcus spp. on ocular surfaces of fighting bulls (Bos indicus) in Thailand

Research (Published online: 24-12-2022)

22. The prevalence and antibiotic susceptibility of Staphylococcus spp. on ocular surfaces of fighting bulls (Bos indicus) in Thailand

Dennapa Saeloh Sotthibandhu, Saowakon Indoung, Husna Niwasawat, Jiradchaya Chaiboon, Nattakan Sungsorn, Nu-issana Longji, Kittipol Polya, Chayanee Noosak, Stefan Schwarz, and Tanawan Soimala

Veterinary World, 15(12): 2922-2928

ABSTRACT

Background and Aim: Fighting bulls have a high risk of eye injuries, and opportunistic conjunctival bacterial flora may cause subsequent eye diseases. There is little information about the ocular health care of fighting bulls in Thailand. Thus, this study aimed to estimate the prevalence of Staphylococcus spp. from the eyes of fighting bulls and investigate their antimicrobial susceptibility.

Materials and Methods: The samples were collected from the right conjunctival sacs of 105 fighting bulls. Biochemical tests and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were used to identify bacteria to genus and species levels. Antimicrobial susceptibility was tested by agar disk diffusion.

Results: Staphylococcus spp. (36.84%, 56/152) were the most detected bacteria. The most prevalent Staphylococcus spp. was Staphylococcus chromogenes (37.50%, 21/56). The susceptibility test revealed that all isolates were susceptible to sulfamethoxazole/trimethoprim (56/56, 100%) and most were susceptible to chloramphenicol and gentamicin (54/56, 96.43%). The highest resistance rates were seen for tetracycline and doxycycline (23.21%, 13/56) followed by erythromycin (19.64%, 11/56). In addition, S. chromogenes isolates were evaluated for their ability to produce biofilms by a quantitative biofilm production assay. A total of 21 isolates exhibited biofilm production, independent of their antimicrobial susceptibility. Three multidrug-resistant isolates were found, including two Staphylococcus epidermidis isolates and a single S. chromogenes isolate.

Conclusion: As antimicrobial resistant bacteria were detected on the eye surface, veterinarians should always conduct antimicrobial susceptibility testing before using antimicrobial agents. The results from this study will help to improve the standard of eye treatment for fighting bulls in Thailand.

Keywords: antimicrobial susceptibility, conjunctival flora, fighting bulls, Staphylococcus spp.

Theileria infection in bullfighting cattle in Thailand

Research (Published online: 23-12-2022)

21. Theileria infection in bullfighting cattle in Thailand

Pirayu Rakwong, Narissara Keawchana, Ruttayaporn Ngasaman, and Ketsarin Kamyingkird

Veterinary World, 15(12): 2917-2921

ABSTRACT

Background and Aim: An apicomplexan protozoan parasite, namely, Theileria, primarily causes theileriosis in cattle worldwide. The virulence of the disease has been neglected because of it's low pathogenicity. However, the disease can have a substantial effect, depending on the virulence of the species, low host immunity, and coinfection. In Thailand, the molecular detection of Theileria infection in bullfighting cattle and its hematological alterations have not been reported. Thus, this study aimed to identify Theileria species in bullfighting cattle in Thailand.

Materials and Methods: Blood samples were collected from bullfighting cattle presented at the Prince of Songkla University Animal Hospital and were determined on the basis of hematological evaluation and DNA extraction. Molecular detection using the 18s rRNA and merozoite surface antigen genes was conducted for Theileria spp. and Theileria orientalis, respectively. In addition, bidirectional sequencing of the positive samples was performed. Hematological alterations between Theileria infected and uninfected groups were statistically evaluated.

Results: The levels of Theileria spp. and T. orientalis infection in bullfighting cattle were 44.62% (58/130) and 41.54% (54/130), respectively. Theileria orientalis, Theileria sinensis, and Theileria spp. infections were identified in bullfighting cattle samples. Hematological evaluation indicated that the red blood cell (RBC) level was significantly lower in Theileria-infected cattle.

Conclusion: This study was the first to use molecular techniques in the identification of Theileria infection in bullfighting cattle in Thailand, with nearly one-half of the study population infected. Theileria infection in bullfighting cattle altered the RBC level, resulting in anemia. Therefore, tick control measures should be promoted.

Keywords: bullfighting cattle, hematological alteration, molecular identification, Thailand, Theileria.

Common diseases of cattle in Jordan: A retrospective study (2015–2021)

Research (Published online: 23-12-2022)

20. Common diseases of cattle in Jordan: A retrospective study (2015–2021)

Myassar Alekish and Zuhair Bani Ismail

Veterinary World, 15(12): 2910-2916

ABSTRACT

Background and Aim: In many developing countries, infectious and non-infectious diseases remain a major hurdle in achieving satisfactory status related to animal health, productivity, and welfare. In Jordan, there are no comprehensive reports describing the most common diseases involving different body systems in different age groups of cattle. Therefore, this retrospective study was designed to report the frequencies of various infectious and non-infectious diseases and their distribution according to sex, age, and body system in cattle in Jordan.

Materials and Methods: Case medical records of cattle presented for clinical evaluation to the Veterinary Health Center of the Faculty of Veterinary Medicine at Jordan University of Science and Technology between January 2015 and December 2021 were used in this study. The data were categorized based on sex (female vs. male), body system involved in the disease process, nature of the disease process (infectious vs. non-infectious), and age (pre-weaning [<2 months of age], 2 months–2 years of age, and older than 2 years of age). Descriptive analysis was performed to report the frequencies, averages, and range values using Excel spreadsheets.

Results: Medical records of 513 cattle cases were used in the study. All cattle belonged to the Holstein-Friesian dairy breed. The majority of cases were female (91%). The age of animals ranged between 1 day and 8 years. According to age groups, there were 52%, 27%, and 16% of cases older than 2 years, 2 months–2 years, and pre-weaning (<2 months), respectively. Among males and females, the majority of cases were diagnosed with gastrointestinal diseases (30%), followed by udder/teat diseases (18%), reproductive and obstetrical diseases (16%), and respiratory diseases (11%). Other body systems involved in disease processes were metabolic (7%), musculoskeletal (6%), cardiovascular/circulatory (4%), multiple systems (3%), nervous (2%), ear/eye (2%), and skin (1%).

Conclusion: Results of this study provide valuable information on the most likely diagnostic list of diseases involving various body systems of different age groups in cattle in Jordan. This information could serve as a clinical guideline for field diagnosis of cattle diseases and provide an accurate estimate of the current status of cattle welfare, health, and husbandry practices in Jordan.

Keywords: animal welfare, cattle diseases, Holstein-Friesian dairy cows, socioeconomic impact of animal disease.