Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells

Research (Published online: 14-07-2022)

9. Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells

Ricaurte Lopera-Vásquez, Fabián Uribe-García and Iang Rondón-Barragán

Veterinary World, 15(7): 1665-1675

ABSTRACT

Background and Aim: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cycle phase. This study aimed to elucidate the effect of estrous cycle phases (follicular and early and late luteal phases) on gene expression patterns in bovine oviduct epithelial cells (BOECs).

Materials and Methods: Oviducts were obtained from healthy slaughterhouse animals, corresponding to ipsilateral ovaries with dominant follicles or corpus luteum during early and late luteal phases. BOECs were recovered from the isthmus (IST) and ampulla (AMP), and the expression patterns of genes related to cytokinesis and mitosis mechanisms (rho-associated coiled-coil containing protein kinase and cellular communication network factor 2 [CCN2]), growth factors (insulin-like growth factor-binding protein 3, epidermal growth factor receptor [EGFR], vascular endothelial growth factor A, and EGFR), antioxidant mechanisms (glutathione peroxidase 4 [GPX4]), apoptosis (B-cell lymphoma 2), complement component (C3), energy metabolism (aldose reductase gene family 1-member b1 [AKRIB1] and solute carrier family 2), hormone receptors (estrogen receptor 1 and luteinizing hormone/choriogonadotropin receptor), and specific glycoproteins (oviductal glycoprotein 1) were analyzed.

Results: High P4 levels (late luteal phase) affected the expression of important genes related to antioxidant mechanisms (GPX4), energy metabolism (AKRIB1), growth factors (IGBP3 and EGFR), and cell growth regulation (CCN2) in the AMP. Low P4 levels (early luteal phase) affected the expression of AKR1B1, IGBP3, and CCN2. In addition, estrogen likely had an effect on OVPGP expression in the cattle oviduct.

Conclusion: Differential gene expression patterns of BOECs in the AMP during the luteal phase (antioxidant mechanisms, energy metabolism, growth factors, and immunological regulators) and in the IST during the follicular phase (glycoproteins) may influence their renewal and population proportions, modulating the oviduct environment as well as gamete and embryo physiology.

Keywords: bovine oviduct epithelial cells, estrus phase, follicular, gene expression, luteal, oviduct.

Effects of dietary betaine on body temperature indices, performance, metabolism, and hematological variables of dairy heifer calves during hot summer conditions

Research (Published online: 14-07-2022)

8. Effects of dietary betaine on body temperature indices, performance, metabolism, and hematological variables of dairy heifer calves during hot summer conditions

Mohmmad Al-Qaisi, Mohamed A. Abedal-Majed, Mohannad Abuajamieh, Mufeed Alnimer, Abdur-Rahman A. Al-Fataftah, Rabie Irshaid, Hosam Titi and Anas Abdelqader

Veterinary World, 15(7): 1657-1664

ABSTRACT

Background and Aim: Heat stress (HS) can negatively impact farm animal productivity and adversely affect animal welfare worldwide, placing a major financial burden on global livestock producers. Dietary betaine (trimethylglycine) has been known to have several biological functions, which may aid in offering beneficial effects on livestock productivity during HS conditions. However, information on the role of dietary betaine in heat-stressed dairy heifer calves is yet to be documented. Therefore, this study aimed to assess the effects of supplementing dietary betaine on body temperature indices, blood metabolites, productive performance, and complete blood count (CBC) (hematological indices) in hyperthermic dairy heifer calves.

Materials and Methods: In total, 14 Holstein heifer calves (4.0 ± 0.9 months old) were individually housed and randomly allocated to one of two dietary treatments: (1) a control diet (CON; n = 7) and (2) a control diet complemented with 21 g/d of natural betaine (BET; n = 7) top-dressed once daily. The experiment lasted for 28 d, during which all animals were subjected to natural cyclic HS conditions (26.1–39.2°C; 73.2–84.0 temperature–humidity index). Rectal temperature (RT) and respiration rate (RR) were measured twice daily (0700 and 1500 h), whereas dry matter intake (DMI) was measured once daily (0800 h). In addition, blood samples (collected from the jugular vein) were analyzed for metabolites and CBC on days 7, 14, 21, and 28.

Results: Relative to CON, BET supplementation was able to decrease RT on day 23 of the experiment (p = 0.04). Alternatively, RR was similar between the dietary treatments (p = 0.73). Feeding BET did not affect DMI compared with CON during HS conditions (p = 0.48). Furthermore, compared with CON, BET supplementation did not change leukocytes, neutrophils, lymphocytes, and hematocrit levels during HS conditions (p ≥ 0.17). However, a post hoc analysis indicated that hematocrit levels were decreased in BET-fed calves on day 7 of the study compared with CON calves during HS conditions (p = 0.05). Moreover, circulating glucose, albumin, and triglycerides were found to be similar between dietary treatments (p ≥ 0.55).

Conclusion: BET supplementation slightly reduced RT and circulating hematocrit but did not affect other metrics in this HS experiment. More research into the effects of different doses of dietary BET on dairy heifer calves is needed.

Keywords: betaine, dairy calves, heat stress.

Identification of cellulolytic lactic acid bacteria from the intestines of laying hens given AKBISprob based on 16S ribosomal ribonucleic acid gene analysis

Research (Published online: 14-07-2022)

7. Identification of cellulolytic lactic acid bacteria from the intestines of laying hens given AKBISprob based on 16S ribosomal ribonucleic acid gene analysis

Nurliana Nurliana, Baharuddin Halim Siregar, Wahyu Eka Sari, Teuku Zahrial Helmi and Sugito Sugito

Veterinary World, 15(7): 1650-1656

ABSTRACT

Background and Aim: Supplementation of AKBISprob (developed in a previous study) in feed can improve production efficiency and poultry health, especially laying hens. In addition, it can also increase cellulolytic lactic acid bacteria (LAB) in chicken intestines, but these bacteria are still unknown; thus, they need to be identified. This study aimed to identify cellulolytic LAB in the intestines of laying hens administered AKBISprob based on 16S ribosomal ribonucleic acid (16S rRNA) gene analysis.

Materials and Methods: The samples used in this study were 13 LAB isolates from the intestines of laying hens that were given AKBISprob 4%. Cellulolytic LAB DNA was isolated and 16S rRNA gene was amplified by polymerase chain reaction, followed by sequencing, bioinformatics analysis, and phylogenetic tree construction.

Results: From 10 cellulolytic LAB isolates with a clear zone of >6 mm, four were selected and their DNA was amplified with BaCF and UniB primers ∼1500 bp DNA fragments. Of these, the P31H62 isolate was genetically close to Enterococcus hirae strain 1-1X-16 with 92.90% maximum identity, the P33S52 isolate had homology with Enterococcus mundtii strain ZU 26 with 96.76% maximum identity, and the P33S62 isolate was closely related to E. hirae strain SJ3 with 72.96% maximum identity. The phylogenetic tree revealed that the cellulolytic LAB isolates P31H62 and P33S52 were in one cluster closely related to the genus Enterococcus.

Conclusion: This study suggests that the isolates P31H62, P33S62, and P33S52 from the intestines of laying hens administered 4% AKBISprob are cellulolytic LAB belonging to the genus Enterococcus.

Keywords: 16S ribosomal ribonucleic acid, AKBISprob, Enterococcus, phylogenetic tree, polymerase chain reaction.

Assessment of selected molecular factors and 17-β estradiol dosage in response to Toxoplasma gondii infection in swine

Research (Published online: 13-07-2022)

6. Assessment of selected molecular factors and 17-β estradiol dosage in response to Toxoplasma gondii infection in swine

Annamaria Castello, Esterina Fazio, Tiziana Alfonzetti, Renato Paolo Giunta, Antonio Salvaggio, Alida Maria Ferlazzo, Cristina Cravana, Giuseppe Bruschetta, Pietro Medica and Anna Maria Fausta Marino

Veterinary World, 15(7): 1641-1649

ABSTRACT

Background and Aim: Toxoplasma gondii is a global zoonotic parasite infecting virtually all warm-blooded species, although a species-specific variability is evident referring to symptoms frame. Both the success of T. gondii and the outcome of infection depend on a delicate balance between host cellular pathways and the evasion or modulation strategies elicited by the parasite. The hormonal and molecular mechanisms involved in this delicate host-parasite balance are still unclear, especially when considering intermediate host species other than mouse. This study aimed to assess any correlation between T. gondii infection and selected molecular and hormonal factors involved in responses to infection in susceptible species such as swine. Moreover, blood counts and hematochemical assays (glucose, total cholesterol, and triglycerides dosage) were performed to evaluate the overall health condition of animals.

Materials and Methods: Toxoplasmosis was diagnosed by enzyme-linked immunosorbent assay for antibodies determination and real-time polymerase chain reaction (RT-PCR) for T. gondii DNA detection. Target genes coding for key factors of cell responses to T. gondii infection were selected, and their transcription was assessed in various tissues by quantitative RT-PCR. 17-β estradiol concentrations were assessed by fluorimetric enzyme-linked immunoassay and the AIA- 360 automated immunoassay analyzer. Blood count and hematochemical analyses were performed by a blood cell counter and a spectrophotometer, respectively.

Results: The present research highlighted significant differences among infected and uninfected swine (control group) for both transcription profiles of some of the molecular factors considered and 17-β estradiol concentrations. Referring to the assessed hematological and biochemical parameters, no statistically significant differences were observed in infected swine compared to the control group.

Conclusion: Our results contribute to the enrichment of data available about the subject and could be useful for a deeper knowledge of the interaction between this parasite and its hosts. However, more aspects are still unclear, such as the effective response of downstream molecules from the same pathways to the variation of factors observed in this study either assessing how the same factors respond to Toxoplasma gondii infection in other host speciesand further analyses should be performed on other host species.

Keywords: biomarkers, host-parasite interaction, swine, Toxoplasma gondii.

Effect of leptin C528T and leptin C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows

Research (Published online: 08-07-2022)

5. Effect of leptin C528T and leptin C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows

K. M. Dzhulamanov, S. V. Lebedev, Nikolay Gerasimov and V. I. Kolpakov

Veterinary World, 15(7): 1632-1640

ABSTRACT

Background and Aim: The leptin (LEP) gene plays a role in the regulation of the activity required to obtain food, energy metabolism, and fat deposition and affects the body composition of animals. Lipogenesis is an ineffective process. A lot of energy from feed is expended on the synthesis of adipose tissue. This study aimed to determine the effect of LEP C528T and LEP C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows.

Materials and Methods: Heifers (n = 49) and first-calf cows (n = 30) were grouped according to their genotype for LEP C528T and LEP C73T polymorphisms at the age of 24 months. DNA samples were isolated from whole blood. Experimental animals were slaughtered at the age of 24 months, and a chemical analysis of samples of longissimus dorsi muscle and ground beef was performed.

Results: The maximum internal fat weight, back-fat thickness, and fat content of the longissimus dorsi muscle and ground beef were determined in heifers heterozygous for both LEP C528T and LEP C73T polymorphisms. The ranking of genotypes in terms of adipose tissue formation did not change in first-calf cows compared to heifers carrying the same LEP gene variants. Pregnancy had a more significant (p < 0.05–0.001) effect on slaughter parameters and lipogenesis in animals of different genotypes than studied polymorphisms. Heterozygosity of the LEP gene was much more strongly expressed in the carcass grade of heifers. "Prime" grades were assigned to 66.7% of carcasses heterozygous for LEP C528T and "Top Choice" to 58.8% of carcasses with LEP C73T nucleotide substitutions.

Conclusion: LEP C528T and LEP C73T polymorphisms were shown to affect the extent of fat formation in Aberdeen Angus heifers and first-calf cows. Animals with heterozygous genotypes exhibited the maximum development of internal fat, back-fat, and intramuscular fat. Pregnancy had a more significant effect on slaughter parameters and adipose tissue formation than studied polymorphisms. First-calf cows had a significantly lower fat content in carcasses than heifers. These results can aid in the production of efficient mature herds of Aberdeen Angus cattle.

Keywords: Aberdeen Angus, beef cattle, genotyping, leptin, polymorphism.

First report of three novel Bartonella species isolated in rodents and shrews from nine provinces of Thailand

Research (Published online: 08-07-2022)

4. First report of three novel Bartonella species isolated in rodents and shrews from nine provinces of Thailand

Decha Pangjai, Burin Nimsuphan, Wimol Petkanchanapong, Wattanapong Wootta, Maskiet Boonyareth, Wuttikon Rodkvamtook and Sumalee Boonmar

Veterinary World, 15(7): 1624-1631

ABSTRACT

Background and Aim: Bartonella spp. are Gram-negative zoonotic bacteria that are transmitted to humans by several types of animal hosts, including rodents. Several studies have been conducted on the prevalence of Bartonella infections in rodents. However, the risk of rodent-associated Bartonella spp. infection in humans remains unclear. This study aimed to estimate the prevalence and genetic heterogeneity of Bartonella spp. in rodents and shrews from nine provinces of Thailand using culture and molecular techniques.

Materials and Methods: A total of 860 blood samples from rodents and shrews across nine provinces of Thailand were collected from January 2013 to June 2016. Bartonella spp. were isolated from all samples using conventional culture techniques and polymerase chain reaction. Phylogenetic tree analysis was used to align the Bartonella sequences obtained from this study.

Results: The prevalence of Bartonella spp. in rodents and shrews was 11.5% (99/860, 95% confidence interval: 9.38–13.64%). The following nine species of Bartonella were detected: Bartonella tribocorum, Bartonella rattimassiliensis, Bartonella queenslandensis, Bartonella elizabethae, Bartonella chanthaburi spp. nov., Bartonella satun spp. nov., Bartonella coopersplainsensis, Bartonella ranong spp. nov., and Bartonella henselae. The prevalence of Bartonella-positive animals differed significantly among provinces.

Conclusion: To the best of our knowledge, the three novel Bartonella spp. isolated from rodents and shrews across Thailand were detected for the first time in this study. Further studies on the epidemiology of Bartonella infection in rodents and its interaction with human health should be conducted in accordance with the Thai government's "One Health" approach to humans, animals, and the environment.

Keywords: Bartonella spp., phylogenetic analysis, polymerase chain reaction, rodents.

Immunogenicity of a newly developed vaccine against Clostridium perfringens alpha-toxin in rabbits and cattle

Research (Published online: 07-07-2022)

3. Immunogenicity of a newly developed vaccine against Clostridium perfringens alpha-toxin in rabbits and cattle

Mohamed J. Saadh, Feras F. Lafi, Adnan A. Dahadha and Mohamed S. Albannan

Veterinary World, 15(7): 1617-1623

ABSTRACT

Background and Aim: Clostridium perfringens type A is an anaerobic bacterium that produces four major toxins (alpha, beta, epsilon, and iota) that cause various diseases. Most of the important C. perfringens-associated diseases of farm animals are caused by alpha-toxin. This study aimed to produce a vaccine against alpha-toxin using C. perfringens type A (ATCC 13124) and investigate its potency, stability, and safety.

Materials and Methods: The vaccine was formulated of its constituents for 1 h. Each milliliter of the final vaccine product contained alpha toxoid 15 lecithovitellinase activity (Lv) by adding (0.375 mL containing 40 Lv) and approximately 0.2 mL from 3% concentrated aluminum hydroxide gel, <0.001% W/V thiomersal, <0.05% W/V formaldehyde, and nearly 0.425 mL phosphate-buffered saline (pH 7.2). The vaccine efficacy was evaluated in rabbits and cattle by performing potency, stability, and safety tests.

Results: The vaccine produced approximately 8.8 and 4.9 IU/mL neutralizing antibodies in rabbits and cattle, respectively. These concentrations were higher than the lowest concentration recommended by various international protocols and the United States Department of Agriculture by 2.20-fold in rabbits and 1.23-fold in cattle. Interestingly, the formulated vaccine enhanced immune responses by 1.80-fold in rabbits compared with that in cattle; the difference was statistically significant (p < 0.0001). The vaccine was stable for 30 months. In vaccinated rabbits, the body temperature slightly increased temporarily during the first 10 h of vaccination; however, the temperature difference was not statistically significant (p > 0.05).

Conclusion: This study describes a manufacturing process to obtain sufficient amounts of a vaccine against C. perfringens alpha-toxin. The formulated vaccine effectively elicited a higher level of neutralizing antibody response than the international standards. Furthermore, the vaccine was found to be stable, safe, and effective in preventing C. perfringens-related diseases in rabbits and cattle. Further studies are necessary to evaluate the efficacy of this vaccine in other farm animals.

Keywords: alpha-toxin, Clostridium perfringens A, potency, safety, stability, toxoid.