Identification and discrimination of Theileria annulata by polymerase chain reaction-restriction fragment length polymorphism

Research (Published online: 14-04-2022)

15. Identification and discrimination of Theileria annulata by polymerase chain reaction-restriction fragment length polymorphism

Abdelfattah Selim and Hanem Khater

Veterinary World, 15(4): 925-929

ABSTRACT

Background and Aim: Theileria annulata infection is a tick-borne disease affecting ruminants in the tropical and subtropical regions causing severe economic losses. This study aimed to characterize circulating T. annulata isolates from four governorates (administrative districts) north and south of Egypt using polymerase chain reaction (PCR)-restriction fragment length polymorphism.

Materials and Methods: Fifty samples were collected from the four governorates of Egypt and were examined by a PCR assay based on the heat shock protein 70 gene. The amplified product was subsequently digested using two restriction enzymes, Taq I and Alu I, to determine which pattern of T. annulata strains was involved.

Results: The findings revealed that one distinct pattern was observed for T. annulata isolates in the northern governorates and another one in the southern governorates. The Taq I enzyme produced three fragments (100, 175, and 270 bp), and the Alu I enzyme produced four fragments (60, 90, 125, and 270 bp).

Conclusion: This study determined the presence of two distinct circulating genotypes of T. annulata among cattle in Egypt based on PCR-RFLP using the HSP 70 gene. More studies are needed in different parts of the country to investigate the virulence and strain variance of T. annulata in cattle.

Keywords: cattle, Egypt, genotype, polymerase chain reaction-restriction fragment length polymorphism, Theileria annulata.

Prevalence and risk factors associated with tropical theileriosis in Egyptian dairy cattle

Research (Published online: 14-04-2022)

14. Prevalence and risk factors associated with tropical theileriosis in Egyptian dairy cattle

Abdelfattah Selim, William Weir and Hanem Khater

Veterinary World, 15(4): 919-924

ABSTRACT

Background and Aim: Theileria annulata is the most prevalent piroplasm infecting cattle across Egypt. Theileriosis is transmitted by ixodid ticks of the genus Hyalomma. This study aimed to estimate the prevalence of theileriosis in cattle and their associated risk factors for infection.

Materials and Methods: A total of 570 blood samples were collected from cattle from five governorates (administrative districts) in Egypt and examined using a polymerase chain reaction assay to estimate the prevalence of tropical theileriosis and assess the associated risk factors.

Results: The overall prevalence rate was 16.49%, with the Alexandria district having the highest prevalence. The results revealed that the risk of theileriosis was elevated in older cattle (odds ratio [OR]=8.9, 95% confidence interval [CI]: 3.6- 21.9), especially in summers (OR=3.07, 95% CI: 1.4-6.3). Cattle heavily infested with ticks were at particular risk (OR=3.05, 95% CI: 2.1-4.5), as were those to which acaricide had not been applied (OR=13.7, 95% CI: 5.6-33.6).

Conclusion: Understanding the risk factors associated with T. annulata infection and regular infection monitoring could reduce infection rates and economic losses and is essential for the implementation of efficient control programs.

Keywords: cattle, Egypt, polymerase chain reaction, risk factors, Theileria annulata.

The effect of bile salt diet supplementation on genes related to fat metabolism in yellow-feathered broilers

Research (Published online: 13-04-2022)

13. The effect of bile salt diet supplementation on genes related to fat metabolism in yellow-feathered broilers

Zhenming Zhang, Baoan Ding, Hailian He, Jingge Wang, Xiongjie Liu, Jiahui Guo, Pengxiang Li and Stephen R. Madigosky

Veterinary World, 15(4): 911-918

ABSTRACT

Background and Aim: As a new feed additive, bile acid (BA) can promote the absorption and transport of lipids and fat-soluble vitamins. In recent years, BAs have been widely used in animal feed to promote fat absorption. Therefore, this study aimed to investigate the effect of bile salt supplementation in the diet of yellow-feathered broilers on messenger RNA (mRNA) expression of sterol regulatory element-binding protein 1 (SREBF1), fatty acid synthase (FAS), acetyl-coenzyme A carboxylase (ACC), and fatty acid transport protein 4 (FATP4).

Materials and Methods: Four hundred and twenty commercial male chicks were randomly divided into seven groups (with four replicates per group and 15 chickens per replicate). They were fed diets supplemented with bile salts at 0, 1.5, 2.5, 3.5, 4.5, 5.5 mg/kg, and 2 mg/kg tylosin for 30 days. Changes in SREBF1, fatty acid transporter 4, FAS, and acetyl- CoA carboxylase genes in intestinal mucosa and liver of yellow-feathered broilers were determined using a quantitative fluorescence polymerase chain reaction.

Results: mRNA expression of SREBF1, FAS, ACC, and FATP4 in the small intestine decreased in chicks fed diets supplemented with 3.5 and 4.5 mg/kg bile salts (p<0.05) compared with the control group on 7 days and 14 d. The mRNA expressions of SREBF1, FAS, ACC, and FATP4 in liver tissue decreased in chicks fed diets supplemented with 4.5 and 5.5 mg/kg bile salts (p<0.05) compared to the control group on 7 days. The mRNA expression of SREBF1, FAS, ACC, and FATP4 in the liver at 14 days and the small intestine on 21 days also decreased in chicks fed diets supplemented with 4.5 mg/kg bile salts (p<0.05) compared to the control group. When contrasted with the control group on day 21, the mRNA expression of SRWBF1, FAS, ACC, and FATP4 detected in the liver was lower in chicks fed diets supplemented with bile salts (p<0.05).

Conclusion: The dietary supplementation of bile salts at 4.5 mg/kg effectively regulates the expression of fat metabolism genes, such as SREBF1, FAS, ACC, and FATP4 mRNA. At this concentration, bile salts promote fat catabolism, inhibit fat synthesis, and play an essential role in improving the fat deposition of broilers.

Keywords: acetyl-CoA carboxylase, fat metabolism, fatty acid synthase, fatty acid transporter 4, sterol regulatory element-binding transcription factor 1, supplemental dietary bile salts.

Feeding of faba beans (Vicia faba L.) enhances the growth performance of lambs

Research (Published online: 12-04-2022)

12. Feeding of faba beans (Vicia faba L.) enhances the growth performance of lambs

Nawras L. Al Shabuol and Belal S. Obeidat

Veterinary World, 15(4): 906-910

ABSTRACT

Background and Aim: The high price of conventional diet ingredients led livestock producers to search for alternative feed sources such as faba beans (Vicia faba L.; FB). This study aimed to evaluate the effect of feeding FB on the growth performance of lambs.

Materials and Methods: A total of 24 male lambs were distributed randomly into two groups and fed a control diet (CON; n=12) and 200 g/kg FB (FB200; n=12) dietary dry matter (DM). The study lasted for 70 days. The first 7 days were used for acclimatization, followed by 63 days of data collection (i.e., nutrient intake and digestibility and growth performance). A complete randomized design was used for the statistical analysis.

Results: The average initial body weight (BW) (20.54±0.798 kg) was similar between the diet treatment groups. Lambs fed the FB200 diet demonstrated higher (p≤0.008) nutrient intake than lambs fed the CON diet. The FB200 diet tended to improve the digestibility of DM, crude protein, and acid detergent fiber more than the CON diet (p≤0.072). Neutral detergent fiber and ether extract digestibility were higher (p<0.05) in lambs fed the FB200 diet than those fed the CON diet. Next, nitrogen retention increased (p<0.05) in lambs fed the FB200 diet compared with the CON diet. Final BW did not differ (p=0.221) between the two groups. However, the average daily gain was higher (p=0.028) in lambs fed the FB200 diet than in lambs fed the CON diet. Furthermore, the cost of gain decreased more (p=0.04) with the FB200 diet than with the CON diet.

Conclusion: The results obtained in this study demonstrate the feasibility of using FB in feeding growing lambs, as it was shown to improve growth performance and reduce the cost of diet and gain.

Keywords: Awassi lambs, faba beans, growth performance, nutrient intake and digestibility.

Efficacy of celery (Apium graveolens L.) alcoholic extract against systemic methicillin-resistant Staphylococcus aureus infection in rat models

Research (Published online: 12-04-2022)

11. Efficacy of celery (Apium graveolens L.) alcoholic extract against systemic methicillin-resistant Staphylococcus aureus infection in rat models

Yos Adi Prakoso and Agustina Dwi Wijayanti

Veterinary World, 15(4): 898-905

ABSTRACT

Background and Aim: The coronavirus disease-19 (COVID-19) pandemic caused global economic and health problems. The pandemic increased the number of infectious diseases categorized as neglected diseases, such as staphylococcosis, which is caused by methicillin-resistant Staphylococcus aureus (MRSA). Celery is an herb that consist of antioxidants that can potentially act as antimicrobial agents. This study aimed to analyze the efficacy of celery alcoholic extract against systemic MRSA infections in rat models.

Materials and Methods: In this study, 36 male, 6-month-old Sprague-Dawley rats (average weight: 300 g) were used as models. The rats were divided into six groups: Group K– (negative control), Group K+ (infected with MRSA without therapy), Group V (infected with MRSA+100 mg vancomycin per kg body weight [BW]), Group P1 (infected with MRSA+1 mg celery extract per kg BW), Group P2 (infected with MRSA+2 mg celery extract per kg BW), and Group P4 (infected with MRSA+4 mg celery extract per kg BW). The therapy was given once daily for 7 days. Blood and organs were taken on day 7 for hematology, serology, immunohistochemistry, and histopathology.

Results: Results showed that 4 mg celery extract per kg BW promotes the healing of MRSA systemic infections in rat models (p≤0.05). The better prognosis was indicated by the normalization of red blood cell indices, white blood cell, neutrophil and lymphocyte counts, Cluster of differentiation 4+, Cluster of differentiation 8+, and Cyclooxygenase-2 expression and absence of severe tissue damage. Celery extracts inhibited MRSA growth in the blood samples.

Conclusion: It can be concluded that celery alcoholic extract can potentially be used as an antimicrobial agent against systemic MRSA infections. A clinical study regarding the efficacy of celery extract must be conducted to ensure its potency against MRSA infections in humans.

Keywords: celery, efficacy, healing, methicillin-resistant Staphylococcus aureus, systemic infection.

Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt

Research (Published online: 11-04-2022)

10. Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt

Shereen S. El-Mohandes, Rasha H. Eid, Ahmad M. Allam, Hala A. A. Abou-Zeina and Mohamed K. Elbayoumy

Veterinary World, 15(4): 890-897

ABSTRACT

Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have become a serious public health hazard worldwide. This importance is derived from the increase of new variants, particularly blaTEM, blaSHV, and blaCTX-M genes. This study aimed to examine ESBL-producing Escherichia coli isolated from different governorates in Egypt from dairy cows infected with subclinical and clinical mastitis.

Materials and Methods: This study examined 207 milk samples for the resistance of isolates against 14 different antibiotics and ran serological identification of ESBL-producing E. coli isolates with complete antibiotic resistance. Genotypic and sequencing analyses of several resistance genes were conducted using a polymerase chain reaction.

Results: E. coli was identified in cases with subclinical mastitis (80.5%) and clinical mastitis (85.7%). ESBL-producing E. coli was isolated from 38.2% of subclinical mastitic milk compared to 39.3% in clinical cases, where O26:k60, O125:k70, and O25:k11 were the serotypes with complete resistance to antibiotics. ESBL-producing E. coli isolates were resistant to cefotaxime, amoxicillin, cloxacillin, oxacillin, rifampicin, and penicillin in 100% but susceptible to amoxicillin and clavulanic acid in 82.5% of the cases. Results also revealed that 51.25%, 52.5%, 66.25%, 77.5% and 60% of ESBL-producing E. coli isolates were responsive to ciprofloxacin, ofloxacin, norfloxacin, levofloxacin, and gentamycin, respectively. The detected genes were registered in GenBank as MW345819.1 and MW345820.1 for the E. coli blaTEM gene and MW295407 for the E. coli blaSHV gene.

Conclusion: This study found ESBL-producing E. coli in mastitic milk samples from Egyptian dairy farms and confirmed the occurrence and circulation of the main antibiotic genes (blaTEM and blaSHV) in the samples. Regular and thorough surveillance of ESBL-producing E. coli and subsequent preventive actions are essential for preventing the spread of these resistance genes in the future, which could pose serious and catastrophic health risks. Authorities should cling to the concept of One Health to minimize the risk of new varieties.

Keywords: antibiotic resistance, Escherichia coli, extended-spectrum β-lactamases, mastitis.

Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide

Research (Published online: 11-04-2022)

9. Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide

Jatuporn Rattanasrisomporn, Chananphat Tantikositruj, Anyarat Thiptara, Warangkana Kitpipit, Ittidet Wichianrat, Autchara Kayan and Chaiwat Boonkaewwan

Veterinary World, 15(4): 885-889

ABSTRACT

Background and Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the release of pro-inflammatory cytokines from chicken peripheral blood mononuclear cells (PBMCs) following lipopolysaccharide (LPS) stimulation.

Materials and Methods: Blood samples were collected from six Betong chickens. To isolate PBMCs, density gradient centrifugation was utilized. PBMC culture in RPMI1640 with 10% fetal bovine serum was stimulated with various concentrations of LPS (0, 0.01, 0.1, and 1.0 μg/mL). The production of TNF-α, IL-1β, and IL-6 was determined using an enzyme-linked immunosorbent assay.

Results: When the PBMCs were cultured for 24 h with varying doses of LPS, there was no significant variation in cell viability. TNF-α, IL-1β, and IL-6 levels were measured in Betong chicken PBMC. The release of these cytokines increased considerably as LPS concentration (0.01-1 μg/mL) increased (p<0.05).

Conclusion: In vitro studies of the chicken immune response, notably the release of pro-inflammatory cytokines, can be conducted using PBMCs obtained from chicken blood.

Keywords: chicken, interleukin-1β, interleukin-6, peripheral blood mononuclear cell, tumor necrosis factor-α.