Levels of insulin-like growth factor in bovine, goat, and sheep milk in different lactation periods: The etiological factor of cancer in humans

Research (Published online: 10-12-2021)

14. Levels of insulin-like growth factor in bovine, goat, and sheep milk in different lactation periods: The etiological factor of cancer in humans

Marian Simonov, Vasyl Vlizlo, Volodymyr Stybel, Ruslan Peleno, Volodymyr Salata, Taras Matviishyn, Mariia Кhimych and Oleksii Gorobei

International Journal of One Health, 7(2): 246-250

ABSTRACT

Background and Aim: Despite the positive aspects of consuming dairy products, there are also some reservations. Recently, a large number of publications have demonstrated convincing evidence of a relationship between the intake of dairy products and the development of cancer in humans. This study aimed to determine the level of insulin-like growth factor [IGF], which can cause cancer in consumers, in the milk of farm animals (cows, goats, and sheep) obtained at different stages of lactation.

Materials and Methods: Enzyme-linked immunosorbent assay and statistical method were used for data processing. The IGF level in milk was determined using an enzyme-linked immunosorbent assay. Milk was collected from cows of Holstein and Ukrainian Black-and-White dairy breed, goats of Alpine and Saanen breed, and sheep of Tsigai and East Friesian breed. Milk samples in cows were collected at the beginning, on the peak, and at the end of lactation.

Results: The obtained results showed that the highest IGF level was detected in milk obtained at the beginning of lactation, but its level depended on the animal species. More specifically, the highest level of the hormone was typical for goat's milk and for cow's milk of Holstein breed. Slightly lower values were revealed in the milk of cows of Black-and-White Ukrainian breed and sheep. During lactation, the absolute content of IGF in the milk of all studied animal species decreased. It should be noted that the most pronounced decrease in the IGF level in milk during lactation was noted in sheep (25-40 times), and minor changes were observed in goats (12-14 times). The results also showed that there were individual variations in the IGF level in milk in farm animals.

Conclusion: The highest IGF level was noted in goat's milk, and the lowest level was observed in sheep's milk. Cow's milk, in this regard, had an intermediate IGF level. The level of this particular hormone depends on the period of lactation and the individual characteristics of the animal. It is advisable to include IGF as one of the assessment factors of dairy products' safety because this hormone can cause cancer in consumers of milk and dairy products.

Keywords: cows, goats, insulin-like growth factor, lactation, milk, sheep.

Rift valley fever in Africa with the emerging interest in Libya

Review (Published online: 09-12-2021)

13. Rift valley fever in Africa with the emerging interest in Libya

Abdusalam S. Mahmoud, Osama K. Sawesi, Osama R. El-Waer and Emad M. Bennour

International Journal of One Health, 7(2): 237-245

ABSTRACT

Rift valley fever (RVF) is an acute vector-borne viral zoonotic disease of domestic and wild ruminants. The RVF virus (RVFV) belonging to the Phlebovirus genus of the Bunyaviridae family causes this disease. Studies have shown that mosquitoes are the vectors that transmit RVFV. Specifically, Aedes and Culex mosquito species are among the many vectors of this virus, which affects not only sheep, goats, buffalo, cattle, and camels but also human beings. Since the 30s of the last century, RVF struck Africa, and to a lesser extent, Asian continents, with subsequent episodes of epizootic, epidemic, and sporadic outbreaks. These outbreaks, therefore, resulted in the cumulative loss of thousands of human lives, thereby disrupting the livestock market or only those with seropositive cases. After that outbreak episode, RVF was not reported in Libya until January 13, 2020, where it was reported for the 1st time in a flock of sheep and goats in the southern region of the country. Although insufficient evidence to support RVF clinical cases among the confirmed seropositive animals exists, neither human cases nor death were reported in Libya. Yet, the overtime expansion of RVF kinetics in the Libyan neighborhoods, in addition to the instability and security vacuum experienced in the country, lack of outbreak preparedness, and the availability of suitable climatic and disease vector factors, makes this country a possible future scene candidate for RVF expansion. Urgently, strengthening veterinary services (VS) and laboratory diagnostic capacities, including improvement of monitoring and surveillance activity programs, should be implemented in areas at risk (where imported animals crossing borders from Libyan neighborhoods and competent vectors are found) at national, sub-national, and regional levels. The Libyan government should also implement a tripartite framework (one health approach) among the veterinary public health, public health authority, and environmental sanitation sectors to implement RVF surveillance protocols, along with an active partnership with competent international bodies (OIE, FAO, and WHO). Therefore, this review comprises the most updated data regarding the epidemiological situation of RVF infections and its socioeconomic impacts on African and Asian continents, and also emphasize the emerging interest of RVF in Libya.

Keywords: Africa, epidemiology, Libya, one health, rift valley fever.

Improving the knowledge of high school students about zoonotic diseases from pets in Medellín-Colombia

Research (Published online: 09-12-2021)

3. Improving the knowledge of high school students about zoonotic diseases from pets in Medellín-Colombia

Natalia Uribe Corrales, Karen Velásquez Giraldo, Catalina María Saldarriaga Garcés and Angie Lorena Navarro Giraldo

Veterinary World, 14(12): 3091-3098

ABSTRACT

Background and Aim: The "One Health" concept is a global strategy that recognizes that public health is related to animal health and the environment; however, the role of domestic animals and their involvement in the transmission of zoonoses is often underestimated. The aim of the study was to evaluate and improve the knowledge about zoonotic diseases of domestic animals in high school students from Medellín, Colombia.

Materials and Methods: A quasi-experimental intra-subject study was carried out. This study was conducted with 11th-grade students from four schools in Medellín, Colombia. A structured multiple-choice questionnaire was used from March 2021 to May 2021. The research had two phases, first, "naive" knowledge and learning. Then, descriptive, association, and comparative analysis were carried out using absolute and relative frequencies, Pearson's Chi-square test, and MacNemar's test with a value of p<0.05 considered statistically significant.

Results: A research poll from 315 students of four private schools found that feeding their pets with raw food and leftovers cooked for human consumption were common practices; the results also show a lack of knowledge of their pets' immunization deworming status. It was understood that when the students were able to identify at least two symptoms of zoonoses, one route of its' transmission and two preventive measures, we found that only 12.49% of the polled students had proper knowledge of the disease in domestic animals. After conducting an educational strategy, the findings show a general increase in knowledge, leading us to accept that the academic approach was adequate to statistically increase the recognition of symptoms, routes of transmission and preventive measures (p=0.00).

Conclusion: The use of the theoretical lecture is effective to improve the understanding of the concept of transmission of diseases from animals to humans; in addition, the results show an increase of knowledge in high school students of the related symptoms, transmission routes, and preventive measures of zoonoses diseases in the region.

Keywords: cats, diseases, dogs, education, zoonoses.

Development of a rapid and reliable high-performance liquid chromatography method for determination of water-soluble vitamins in veterinary feed premix

Research (Published online: 08-12-2021)

2. Development of a rapid and reliable high-performance liquid chromatography method for determination of water-soluble vitamins in veterinary feed premix

Md. Zahangir Hosain, S. M. Shariful Islam and Md. Mostofa Kamal

Veterinary World, 14(12): 3084-3090

ABSTRACT

Background and Aim: Determination of trace amounts of vitamins in multi-component feed premix is a troublesome analytical procedure. In this study, a simple and rapid high-performance liquid chromatography (HPLC) method was developed and validated for the concurrent detection and quantitation of four water-soluble vitamins such as thiamine, riboflavin, pyridoxine, and cyanocobalamin in veterinary feed premixes.

Materials and Methods: The chromatographic separation of the vitamins was carried out at 35°C temperature on a reversed-phase C18 column using a gradient pump mode. Mobile phase constituents were solvent (a): 25 mM Potassium dihydrogen phosphate and 5 mM sodium hexanesulfonate in deionized water having pH-4.0 and solvent and (b) 5 mM sodium hexanesulfonate in methanol. Detection was performed with HPLC ultraviolet/visible detection set at 278 and 361 nm wavelength in two different channels. The flow rate was 1.2 mL/min and the total run time was 25 min.

Results: The method was validated according to the International Conference on Harmonization and Food and Drug Administration guidelines and acceptance criteria for system suitability, precision, linearity, and recovery were met in all cases. The relative standard deviation for system suitability and precision was <2% for all vitamins. The linearity of the calibration curves was excellent (R2>0.999) at concentration of 5, 10, 15, 20, 25, and 30 μg/mL for all vitamins. The limits of detection values were 0.0125, 0.0017, 0.0064, and 0.0065 μg/mL for thiamine, riboflavin, pyridoxine, and cyanocobalamin, respectively, and the limits of quantification values were 0.0378, 0.0051, 0.0213, and 0.0198 μg/mL for thiamine, riboflavin, pyridoxine, and cyanocobalamin, respectively. The recovery percentages ranged from 88% to 115%.

Conclusion: The overall parameters of the proposed method met the validation criteria and this method could be a highly desirable technique for routine analysis of water-soluble vitamins in veterinary feed premix.

Keywords: method development, veterinary feed-premix, water-soluble vitamin.

Antioxidant activity and protective effect of propolis against carbon tetrachloride-induced liver and kidney injury by modulation of oxidative parameters

Research (Published online: 08-12-2021)

1. Antioxidant activity and protective effect of propolis against carbon tetrachloride-induced liver and kidney injury by modulation of oxidative parameters

Redouan El-Haskoury, Noori Al-Waili, Zeineb Kamoun, Mohamed Makni, Ahmed Al-Waili and Badiaa Lyoussi

Veterinary World, 14(12): 3076-3083

ABSTRACT

Background and Aim: Propolis has a protective effect against cellular damage caused by toxic agents such as drugs, metals, xenobiotics, and chemicals. The aim of this study was to investigate the antioxidant activity and the effect of ethanolic extract of propolis on carbon tetrachloride (CCl4)-induced oxidative stress on kidney and liver injury in rat.

Materials and Methods: The study quantified phenol, flavone, and flavonol in propolis and assessed antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and molybdate. The investigators used four groups of rats to study the effect of propolis on CCl4-induced toxicity. Propolis extract was given orally (500 mg/kg) for 12 days, and CCl4 (1 mL/kg) was administered intraperitoneally on day 5 of the experiment. Blood and tissue samples of the liver and kidney were collected on day 13 to measure biochemical and oxidative parameters. The parameters included malondialdehyde (MDA), protein carbonyl formation (PCO), advanced oxidation protein products (AOPP), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and ascorbic acid (AA). Biochemical parameters included liver enzymes, blood urea (BU), creatinine, and uric acid (UA).

Results: CCl4 decreased antioxidant agents, including CAT, GPx, GSH, and AA in the liver and kidney tissues. The oxidative agents' levels, including MDA, PCO, and AOPP, increased by CCl4 compared to the control group. CCl4 increased liver enzymes, UA, BU, and creatinine in the blood samples. Propolis significantly alleviated liver and kidney function, improved antioxidant parameters, and decreased levels of oxidative agents.

Conclusion: The data showed for the 1st time that Moroccan propolis has a protective effect against CCl4-induced kidney and liver toxicity by maintaining the activity of the antioxidant defense system, which was most likely due to its antioxidant activity.

Keywords: antioxidant, carbon tetrachloride, kidney, liver, propolis, toxicity.

Prevalence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics, Surabaya, Indonesia

Research (Published online: 07-12-2021)

12. Prevalence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics, Surabaya, Indonesia

Luviana Kristianingtyas, Mustofa Helmi Effendi, Adiana Mutamsari Witaningrum, Dhandy Koesoemo Wardhana and Emmanuel Nnabuike Ugbo

International Journal of One Health, 7(2): 232-236

ABSTRACT

Background and Aim: The practice of keeping animals as pets is becoming increasingly common. The upsurge of extended-spectrum β-lactamase (ESBL)-producing organisms of animal origin is a health threat globally. This study aimed to identify the presence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics in Surabaya, Indonesia.

Materials and Methods: A total of 85 rectal swab samples were collected from companion dogs at five animal clinics in different regions of Surabaya, Indonesia. The presence of E. coli was identified from the samples using standard methods, followed by antibiotic sensitivity testing. The resistant isolates were examined for the presence of ESBL using the double-disk synergy test method. The phenotypically identified ESBL-producing E. coli was further confirmed with an automated system using Vitek-2.

Results: The rectal swab samples (n=85) tested were 100% positive for E. coli isolates. Eight (9.41%) out of the 85 E. coli obtained from rectal swabs were extended-spectrum β-lactamase producers. All eight ESBL-producing E. coli were identified by automated Vitek-2 confirmatory tests.

Conclusion: This study provides insight into the prevalence of ESBL-producing organisms isolated from companion dogs in Indonesia. This work indicates the need for the general public to be more aware of the role of companion animals in disseminating pathogenic organisms, since they serve as potential reservoirs in the spread of antibiotic resistance affecting human health.

Keywords: animal clinics, companion dogs, extended-spectrum β-lactamase, Escherichia coli, human health.

Bovine zoonoses in Cameroon's West region: Knowledge, attitudes, and practices of herdsmen

Research (Published online: 06-12-2021)

11. Bovine zoonoses in Cameroon's West region: Knowledge, attitudes, and practices of herdsmen

Kouengoua Kouengoua Armelle Prudence, Nankam Chimi Roland, Oyetola Wilfried Délé, Njayou Ngapagna Arouna, Alambedji Rianatou Bada and Jean-Pierre Vaillancourt

International Journal of One Health, 7(2): 227-231

ABSTRACT

Background and Aim: Shepherds are in constant contact with animals, and they are potential hosts for the transmission of zoonoses. This study aimed to assess the perceptions of shepherds in Cameroon's West region regarding zoonotic diseases.

Materials and Methods: A cross-sectional study was conducted from August to October 2017, on 218 shepherds. The data were collected using semi-structured interviewer-administered questionnaires. The participants were assessed on their knowledge, attitudes, and practices that might expose them to bovine zoonose transmission, and the data were analyzed with R, version 2.13.0. Descriptive statistics were generated, and the association between socio-demographic characteristics and knowledge was explored using the Chi-square test.

Results: Out of the 218 herdsmen, (205/218) 94% did not have any education, and (195/218) 89.5% did not know the definition of zoonosis. There was a correlation between the level of education and knowledge of the definition of zoonosis: About (162/218) 74.6% cited tuberculosis as a zoonosis, (62/218) 28.4% said the main modes of transmission were by ingestion of contaminated milk, and (66/218) 30.3% by consumption of contaminated meat. Abortions during the third trimester of gestation, combined with the presence of hygroma, were described by (130/218) 59.6% of the participants as brucellosis symptoms. In terms of practices and attitudes, (187/218) 85.8% and (50.14/218) 23.0% of the participants confirmed that they consumed raw milk and raw meat, respectively. Almost all (214/218) 98.1% interviewees stated that blood consumption is forbidden. In case of suspicion of disease, (204/218) 93.6% maintained that they treated sick animals without a veterinary prescription, and (209/218) 95.9% left the carcasses in the wild. After contact with animals, (181/218) 83.3% washed their hands. Finally, (192/218) 88.3% would like to be informed about zoonoses.

Conclusion: The level of knowledge about zoonotic diseases was low; a high percentage of shepherds followed inadequate hygiene practices and expressed concern about zoonoses. As a result, they are at risk of exposure to pathogens. It would be in the interest of public health to develop training programs for shepherds and livestock owners to mitigate zoonotic risks.

Keywords: knowledge, attitudes, and practices survey, shepherds, West region of Cameroon, zoonoses.